Some glucosidase inhibitors show antitumor metastasis and anti-HIV activities and are also clinically useful for the treatment of diabetes,besides andrographolide derivative have been proven to be potent an α-glucosi...
详细信息
Some glucosidase inhibitors show antitumor metastasis and anti-HIV activities and are also clinically useful for the treatment of diabetes,besides andrographolide derivative have been proven to be potent an α-glucosidase *** order to investigate the double effect of glucosidase inhibition activity,a new diterpene lactone derivative 2 was synthesized via reduction of compound 1 which was one of the constitutions of Andrographis Pamicutate(Brum) *** structure of compound 2 was confirmed with X-ray diffraction analysis,NMR,IR,HR-MS and so *** 2 shows a selective α-glucosidase inhibitory activity with inhibition of 49.6% at 100 μmol/L,and the mother 1 has no inhibition activity(100 μmol/L).The results indicate that the double bond can affect the α-glucosidase inhibition activity of andrographolide.
采用高效液相色谱/串联质谱技术建立了快速鉴定Z型盐酸头孢吡肟原料药中E型异构体杂质的新方法。以乙腈-醋酸盐缓冲溶液(4∶96)为流动相经C18柱分离,通过高分辨电喷雾串联质谱在线检测,获得了相关的色谱和质谱信息。在所建立条件下,Z型盐酸头孢吡肟及其E型异构体杂质获得了有效分离,保留时间分别为5.22 m in和14.60 m in,同时它们的二级质谱及裂解方式呈现明显差异。本法能在无对照品情况下,快速、准确地分离鉴定盐酸头孢吡肟原料药中的Z/E异构体。
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