间变型弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)是一种罕见的非特指型DLBCL,组织形态学常为窦性或者弥漫生长。该文报道1例,其左侧腋窝淋巴结具有大量多形性的中心母细胞样伴间变特征的细胞和HRS样细胞呈结节状或滤泡...
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间变型弥漫大B细胞淋巴瘤(diffuse large B-cell lymphoma,DLBCL)是一种罕见的非特指型DLBCL,组织形态学常为窦性或者弥漫生长。该文报道1例,其左侧腋窝淋巴结具有大量多形性的中心母细胞样伴间变特征的细胞和HRS样细胞呈结节状或滤泡生发中心样生长,符合DLBCL,非特殊类型,间变型。结合相关文献探讨其临床病理学及分子遗传学特征,以提高临床和病理医师对该肿瘤的认识。
目的拟通过单细胞转录组测序技术(single-cell RNA sequencing,scRNA-seq)解析移植前体外培养人胸腺组织切片的残余细胞类型和功能,并利用培养上清液分子标志物的水平特征建立胸腺组织切片质量评估方法。方法收集2023年5月至2024年1月在重庆医科大学附属儿童医院心胸外科接受心脏外科手术的18例先天心脏病患者废弃的胸腺制备成胸腺组织切片。体外培养14 d后,通过scRNA-seq鉴定残余的细胞类型,联合基因本体论(gene ontology,GO)和京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)富集分析残余细胞的功能,并查阅胸腺组织切片培养的相关文献筛选出指示胸腺细胞功能的分子标志物。采用ELISA检测上清液分子标志物的蛋白水平变化,绘制受试者工作特征曲线(receiver operating characteristic curve,ROC),以曲线下面积(area under the curve,AUC)判定上清液分子标志物对胸腺组织切片质量的评估价值。将分子标志物判定为质量检测合格与质量检测不合格的胸腺组织切片移植至6~8周龄Balb/c-nude雄性小鼠(体质量14~17 g)皮下(对照组不移植胸腺组织切片),通过流式细胞术和组织学检测分析移植后免疫重建效果。结果(1)scRNA-seq数据显示,胸腺组织切片中含有11种细胞,主要细胞为上皮细胞、成纤维细胞和T细胞。GO和KEGG富集分析显示,上皮细胞与趋化作用、上皮细胞发育、细胞基质粘附、紧密连接等条目相关;成纤维细胞主要与细胞外基质组织、上皮细胞增殖、负向调控细胞迁移、肌动蛋白细胞骨架调节等条目相关;T细胞主要与T细胞分化、T细胞活化的调控、T细胞凋亡、T细胞受体信号等条目相关。(2)筛选出CCL19、CCL21、CXCL12、CXCL16、IL16、SELL作为指示胸腺细胞功能的分子标志物,与第1天相比,CCL19、CCL21、CXCL12和CXCL16蛋白分泌量伴随体外培养显著增加(P<0.05),IL16和L-selectin(SELL表达分泌的蛋白)蛋白分泌量伴随体外培养显著下降(P<0.05)。联合IL16和L-selectin生成预测因子Pre1评估体外培养1 d的胸腺组织切片质量的价值最高,ROC曲线下面积为0.883。联合CCL19、CCL21、CXCL12、CXCL16生成预测因子Pre2评估体外培养14 d的胸腺组织切片质量的价值最高,ROC曲线下面积为0.948。(3)裸鼠移植实验证实:与对照组相比,质量检测合格的胸腺组织切片能在体内发育成胸腺结构,并有效提升裸鼠外周血中T细胞的比例(P<0.01),而质量检测不合格的胸腺组织切片移植到裸鼠体内无法重建裸鼠的T细胞发育。结论移植前胸腺组织切片残留的组成细胞主要为上皮细胞、成纤维细胞和T细胞;IL16和L-selectin可作为判定胸腺原料质量的潜在指标。CCL19、CCL21、CXCL12和CXCL16可有效评估胸腺组织切片成品的质量。
铁死亡是一种新发现的程序性细胞死亡形式,其特征是铁过载及脂质过氧化。越来越多的证据表明,铁死亡与妊娠相关疾病的发生密切相关有关,而抑制铁死亡对于妊娠相关疾病的治疗具有一定作用。本综述总结了铁死亡发生的分子机制及铁死亡在妊娠相关疾病中的最新研究进展,期望对妊娠相关疾病的诊治带来新的思路。Ferroptosis is a newly discovered form of programmed cell death characterized by iron overload and lipid peroxidation. Accumulating evidence suggests a significant association between ferroptosis and the pathogenesis of pregnancy-related disorders. Studies have shown that inhibiting ferroptosis may offer therapeutic benefits in managing these conditions. This review aims to elucidate molecular mechanisms underlying ferroptosis and to summarize recent advancements in understanding its role in pregnancy-related diseases, thereby providing novel insights for the diagnosis and treatment of such disorders.
目的:探讨我国老年人健康行为对慢性病共病的影响并利用关联规则探索共病模式,为制定健康相关行为预防策略提供参考依据。方法:基于中国老年健康影响因素跟踪调查(CLHLS)中10,747名65岁老年人的相关数据,利用多因素logistic回归模型分析健康生活相关行为对慢性病共病的影响;通过关联规则分析慢性病共病组合。结果:研究对象 ≥ 65岁共计10,381名,慢性病共病患病率为36.04%。基于睡眠质量、睡眠时间、健康饮食、无吸烟史、无饮酒史、锻炼以及户外社交活动等7个健康生活相关行为变量的得分,分为健康行为组别人群3023人(29.12%)和不健康行为组别7358人(70.88%)。多因素logistic回归分析结果显示,年龄组别为65~74岁(OR = 1.16, 95%CI: 1.02~1.32)和75~84岁人群(OR = 1.56, 95%CI: 1.40~1.74)、户籍为城市(OR = 2.50, 95%CI: 2.26~2.75)人群是慢性病共病的危险因素。男性、学历组别为文盲和小学、正常BMI以及健康行为是慢性病共病的保护因素。不同健康行为组别的共病组合存在差异。健康生活方式与慢性病共病低风险相关,从健康行为上对老年人加以引导和宣传并加强常见共病模式慢性病的筛查与预防不失为有效降低慢性病共病的途径。Objective: To explore the influence of health behaviors on chronic disease comorbidities among elderly in China and the comorbidity patterns using association rules, to provide a reference for formulating health-related behavior prevention strategies. Methods: Based on the data of 10,747 65-year-old elderly people in the Chinese Longitudinal Healthy Longevity Survey (CLHLS), a multivariate logistic regression model was used to analyze the influence of healthy life-related behaviors on chronic disease comorbidities. The combination of chronic diseases comorbidities was analyzed by association rules. Results: There were 10,381 subjects ≥ 65 years old, and the prevalence of chronic diseases was 36.04%. Based on the scores of 7 behavioral variables related to a healthy life, including sleep quality, sleep time, healthy diet, no smoking history, no drinking history, exercise, and outdoor social activities, the population was divided into 3023 people (29.12%) in the healthy behavior group and 7358 people (70.88%) in the unhealthy behavior group. Multivariate logistic regression analysis showed that the age group of 65~74 years old (OR = 1.16, 95%CI: 1.02~1.32) and 75~84 years old population (OR = 1.56, 95%CI: 1.40~1.74), and the household registration of urban (OR = 2.50, 95%CI: 2.26~2.75) were risk factors for chronic disease comorbidity. Male, educational group illiteracy and primary school, normal BMI, and healthy
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