为了解释多发性硬化(multiple sclerosis,MS)的临床和病理之间的矛盾,引入了功能MRI检查,其中磁共振波谱(proton magnetic resonance spectroscopy,1^H-MRS)检查为其中最重要的技术之一。1^H-MRS对MS脑内病灶提供了一种无创性测量方法,...
详细信息
为了解释多发性硬化(multiple sclerosis,MS)的临床和病理之间的矛盾,引入了功能MRI检查,其中磁共振波谱(proton magnetic resonance spectroscopy,1^H-MRS)检查为其中最重要的技术之一。1^H-MRS对MS脑内病灶提供了一种无创性测量方法,波谱数据分别代表了MS特有的相关事件如脱髓鞘、炎性反应、轴索或神经元的破坏。1^H-MRS研究将MS病变从白质延伸到了灰质,[第一段]
目的通过观察细胞外信号传导激酶(extracelluar signal regulated protein kinase,ERK)及其磷酸化产物p-ERK1/2在耐药性癫患者脑组织中的表达,探讨其在癫发生、发展中的作用。方法免疫组织化学及Western blot法检测32例耐药性颞叶癫...
详细信息
目的通过观察细胞外信号传导激酶(extracelluar signal regulated protein kinase,ERK)及其磷酸化产物p-ERK1/2在耐药性癫患者脑组织中的表达,探讨其在癫发生、发展中的作用。方法免疫组织化学及Western blot法检测32例耐药性颞叶癫(drug-resitstance of temporal lobeepilepsy,DR-TLE)患者(颞叶24例,海马8例)手术标本中ERK1、ERK2、p-ERK1/2(thr202/thr204)的表达,并与12例对照组(颞叶9例,海马3例)进行比较。结果ERK1、ERK2、p-ERK在颞叶中的神经元和胶质细胞中均有表达,且DR-TLE中表达(0.2266±0.0613、0.2097±0.0183和0.1924±0.0054)高于对照组(0.1840±0.0023、0.1974±0.0056和0.1825±0.0063,P<0.05),Western blot分析发现同样的结果。结论ERK及其磷酸化产物在DR-TLE中表达上调,提示ERK信号传导途径可能在DR-TLE发生、发展中起一定作用。
Objective Nitric oxide （NO） was speculated to play an Minocycline, a tetracycline derivative, reduced inflammation important role in the pathophysiology of cerebral ischemia. and protected against cerebral ischemia....
详细信息
Objective Nitric oxide （NO） was speculated to play an Minocycline, a tetracycline derivative, reduced inflammation important role in the pathophysiology of cerebral ischemia. and protected against cerebral ischemia. To study the neuroprotection mechanism of minocycline for vascular dementia, the influences of minocycline on expressions of inducible nitric oxide synthase （iNOS） and endothelial nitric oxide synthase （eNOS） were observed in the brains of Wistar rats. Methods The vascular dementia rat model was established by permanent bilateral common carotid arteries occlusion （BCCAO）. Wistar rats were divideded into 3 groups randomly： sham-operation group （S group）, vascular dementia model group （M group）, and minocycline treatment group （MT group）. The behaviour was tested with Morris water maze and open-field task. Expressions of iNOS and eNOS were measured by immunohistochemistry and reverse transcriptase-polymerase chain reaction （RT-PCR）. The optical density value was measured by imaging analysis. Percentage of positive ceils with iNOS and eNOS expression was analyzed with optical microscope. Results Minocycline attenuated cognitive impairment. Inducible NOS was significantly down-regulated in MT group, compared with that in M group （P 〈 0.01）, while eNOS was significantly up-regulated, compared with that in M group （P 〈 0.01）. The expressions of iNOS and eNOS in M and MT groups were higher than those in S group （P 〈 0.01）. Conclusion Minocycline can down-regulate the expression of iNOS and up-regulate the expression of eNOS in vascular dementia, which restrains apoptosis and oxidative stress to protect neural function.
暂无评论