Objective To establish an artificial somatic-autonomic reflex arc in rats and observe the following distributive changes of neural fibers in the bladder. Methods Adult Sprague-Dawley rats were randomly divided into th...
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Objective To establish an artificial somatic-autonomic reflex arc in rats and observe the following distributive changes of neural fibers in the bladder. Methods Adult Sprague-Dawley rats were randomly divided into three groups： control group, spinal cord injury （SCI） group, and reinnervation group. DiI retrograde tracing was used to verify establishment of the model and to investigate the transport function of the regenerated efferent axons in the new reflex arc. Choline acetyltransferase （CHAT） in the DiI-labeled neurons was detected by immunohistochemistry. Distribution of neural fibers in the bladder was observed by acetylcholine esterase staining. Results DR-labeled neurons distributed mainly in the left ventral horn from L3 to L5, and some of them were also CHAT-positive. The neural fibers in the bladder detrusor reduced remarkably in the SCI group compared with the control （P 〈 0.05）. After establishment of the somatic-autonomic reflex arc in the reinnervation group, the number of ipsilateral fibers in the bladder increased markedly compared with the SCI group （P 〈 0.05）, though still much less than that in the control （P 〈 0.05）. Conclusion The efferent branches of the somatic nerves may grow and replace the parasympathetic preganglionic axons through axonal regeneration. Acetylcholine is still the major neurotransmitter of the new reflex arc. The controllability of detrusor may be promoted when it is reinnervated by the pelvic ganglia efferent somatic motor fibers from the postganglionic axons.
act-Our previous study showed much Id2-immunore-activity in white matter region, ninety percent of Id2 immunoreac-tivity in oligdendrocyte lineage cells in adult rat brain. But whetherit have a function in oligodendro...
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act-Our previous study showed much Id2-immunore-activity in white matter region, ninety percent of Id2 immunoreac-tivity in oligdendrocyte lineage cells in adult rat brain. But whetherit have a function in oligodendrocyte development is unknown,
目前在人和动物已发现有8个信号的转录因子和活化子(signal transducers and activators of transcription,STAT)其中7个在哺乳动物,分别为STAT1,2,3,4,5a,5b,6。研究表明,STAT可参与调节多种信号转导途径,与多种疾病的病理机制相关。...
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目前在人和动物已发现有8个信号的转录因子和活化子(signal transducers and activators of transcription,STAT)其中7个在哺乳动物,分别为STAT1,2,3,4,5a,5b,6。研究表明,STAT可参与调节多种信号转导途径,与多种疾病的病理机制相关。研究多种疾病常选用大鼠作为动物模型,
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