Hypoxia-inducible gene expression is related to interaction between oxygen response/ oxygen regulated elements located in the promoter and/or enhancer and trans-acting factors. 3’Flanking Sequence of erythropoietin (...
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Hypoxia-inducible gene expression is related to interaction between oxygen response/ oxygen regulated elements located in the promoter and/or enhancer and trans-acting factors. 3’Flanking Sequence of erythropoietin (EPO) gene contalns hypoxia inducible enhancer, a 50 bp fragment. The homologous sequence to hypoxia inducible enhancer(HIE) of EPO gene also exists in other hypoxia inducible genes. Hypoxia-inducible factor-1 (HIF-1), isolated from nuclear extract of some cells exposed to hypoxia,is a heterodimer with DNA binding activity and basic-helix-loop-helixPAS domain,and is comPosed of 120kD HIF-1α and 91-94 kD HIF-19. HIF-1 can specifically bind to site l in 3’-HIE of EPO gene induces expression of the gene in response to hypoxia.
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