肝细胞癌(hepatocellular carcinoma,HCC)侵袭转移的机制问题一直是医学界近年来研究的重点。研究发现,HCC中癌细胞的扩散可能与非可控性炎症形成、上皮-间质转化(epithelial-mesenchymal transition,EMT)、免疫逃逸以及微血管生成等过程密不可分。大量研究证明激活蛋白1(activating protein 1,AP-1)是哺乳动物体内重要的转录因子,当机体受到内源性或外源性刺激后可促使其过度激活。过度激活的AP-1在DNA结合域、转录调控域、寡聚化位点和核定位区等结构的紧密配合下促进细胞增殖、分化、凋亡以及癌性转化。本文就AP-1在HCC侵袭转移中的调控作用及其治疗策略的研究进展进行综述。
研究生教育是我国培养高素质人才的重要环节。在医学院校,分子生物学实验课程作为衔接理论知识与实际应用的纽带,对研究生的临床实践、科学研究能力的培养具有重要意义。我们在多年教学经验的基础上,在研究生实验课程中引入了PBL(problem based learning)教学模式,以提出科学问题的方式,将实验中涉及到的质粒DNA提取、核酸电泳、蛋白印迹、细胞转染等分子生物学实验技术进行整合,增加了学生学习的热情,同时锻炼并培养了学生的提出问题、解决问题的科研思维能力。
Objective To investigate the neuroprotective action of astragaloside Ⅳ(AS-Ⅳ) on spatial learning and memory impairment induced by amyloid-beta 1-42(Aβ1-42) in rats and elucidate its underlying molecular *** Adult-m...
详细信息
Objective To investigate the neuroprotective action of astragaloside Ⅳ(AS-Ⅳ) on spatial learning and memory impairment induced by amyloid-beta 1-42(Aβ1-42) in rats and elucidate its underlying molecular *** Adult-male Sprague-Dawley rats(230-250 g) were divided into six groups randomly: control, Aβ1-42, AS-Ⅳ, Aβ1-42 plus 5 mg/kg·d AS-Ⅳ, Aβ1-42 plus 25 mg/kg·d AS-Ⅳ, and Aβ1-42 plus 50 mg/kg·d AS-Ⅳ groups. Aβ1-42 were delivered by intracerebroventricular injection under the guidance of a brain stereotaxic apparatus. The Morris water maze test(hidden platform test, probe trials, visible platform test) was performed one week after Aβ1-42 injection to obtain the ability of rat spatial learning and memory. AS-Ⅳ(5, 25 and 50 mg/kg·d) was administrated intraperitoneally once per day from the 8 th day after Aβ1-42 injection for 5 consecutive days. Average escape latencies, distances for searching for the platform under water and the percentage of total time elapsed and distance swam in the right quadrant after removing platform were determined by behavior softwaresystem. The vision and swim speeds of rats were also determined to exclude the effect of these factors on the parameters of learning and memory. After behavioral tests, the rats were sacrificed immediately by decapitation. Hippocampus were collected. The enzyme activities of superoxide dismutase(SOD), glutathione peroxidase(GSH-px) and catalase(CAT) in the hippocampus obtained from different-treated rat brain were measured by following the manufacturer’s instructions. The levels of interleukin-1 beta(IL-1β) and tumor necrosis factor-alpha(TNF-α) in tissue lysates were assayed with *** The water maze test results indicated that chronic treatments with AS-Ⅳ effectively protected the rats from Aβ1-42-induced spatial learning and memory impairment. Furthermore, the activities of SOD, GSH-px and CAT decreased by Aβ1-42 were also restored by AS-Ⅳ treatment in the hippocampus of rats. In addition,
暂无评论