为了提高香椿(Toona Sinensis)叶总黄酮的纯化效率,试验以鄂东地区野生香椿为材料,采用响应面法建立了H-107大孔树脂对香椿叶中黄酮动态吸附和解吸的二次多项数学模型,并验证了模型的有效性。结果表明,H-107大孔树脂对香椿叶总黄酮动态吸附参数为上样液浓度1.60 mg/m L、上样液流速0.5 m L/min、上样液pH 5.0。H-107大孔树脂的最优动态吸附率验证值为98.7%(理论值为99.1%);H-107大孔树脂对香椿叶中黄酮动态解吸率的工艺参数为解吸液体积分数64.88%、解吸液流速0.91 m L/min、解吸液体积99.11 m L、H-107大孔树脂的最优动态解吸率验证值为87.21%(理论值为88.45%)。上述结果表明,H-107大孔树脂对香椿叶中总黄酮有较好的吸附解吸性能。
This study was conducted to extract pachyman to the greatest degree with ultrasonic technology, and to determine the content of pachyman by the phe-nol-sulfuric acid method, so as to choose the optimal technique of ul...
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This study was conducted to extract pachyman to the greatest degree with ultrasonic technology, and to determine the content of pachyman by the phe-nol-sulfuric acid method, so as to choose the optimal technique of ultrasonic extrac-tion of pachyman. Firstly, the ultrasonic power, extraction time and solid-to-liquid ra-tio were investigated by ultrasonic extraction method. Then, on this basis, a respon-sible surface experiment was designed, to select the optimal process conditions ac-cording to the index of pachyman yield. The optimized ultrasonic water extraction conditions were as fol ow： ultrasonic power of 100 W, extraction time of 30 min, and solid-to-liquid ratio at 1：40. The Oxford cup method was used to study the an-timicrobial activity of pachyman. lt was found that pachyman could significantly in-hibit Bacilus subtilis_and Staphylococcus aureus.
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