Objective To test the hypothesis that neuronal cdc2 like kinase (Cdk5/p35 nck5a ) plays an important role in neuronal maturation and sprouting Methods Changes kinase activity, expression levels and subcellular...
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Objective To test the hypothesis that neuronal cdc2 like kinase (Cdk5/p35 nck5a ) plays an important role in neuronal maturation and sprouting Methods Changes kinase activity, expression levels and subcellular localizations of Cdk5 and p35 nck5a in the rat hippocampus were studied during kindling progression by Western blot analysis, immunohistochemitry, immunoprecipitation and kinase assay Results Kinase activity in kindling rats was significantly higher than that in normal adult rats The kinase activity at stage 3 was most prominent among all stages of kindling progression The changes in kinase activity coincided with those of p35 nck5a expression in kindling rats In contrast, the expression of Cdk5 was constant throughout the progression of kindling stages However, subcellular localization of Cdk5 dramatically changed in the hippocampal neurons of kindling rats Cdk5 was translocated from axon to soma when kinase activity was high p35 nck5a was always localized in the soma throughout kindling progression Conclusions Neuronal cdc2 like kinase plays an important role in synaptic reorganization, and the translocation of Cdk5 to the soma from the axon may be a novel regulatory mechanism to control kinase activity
OBJECTIVE: To investigate gene expression of three nitric oxide synthase isozymes in injured spinal cord tissue. METHODS: Thirty-six adult SD rats were randomly divided into six groups: a normal group and five injury ...
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OBJECTIVE: To investigate gene expression of three nitric oxide synthase isozymes in injured spinal cord tissue. METHODS: Thirty-six adult SD rats were randomly divided into six groups: a normal group and five injury groups, with six per each group. Animals in the injury groups were sacrificed at 2, 6, 12, 24, 48 h after injury. A compression injury model on the spinal cord was made according to Nystrom B et al and gene expression of the three NOS isozymes were examined by reverse transcription polymerase chain reaction (RT-PCR). RESULTS: Gene expression of nNOS and eNOS were detectable in the normal group and were up-regulated quickly after injury, reaching a maximum at 6 h: (0.633 +/- 0.012) and (1.236 +/- 0.207). Gene expression of iNOS was detectable only in the injury groups and it was gradually up-regulated after injury, reaching a maximum at 24 h: (1.043 +/- 0.049). CONCLUSION: Injury to the spinal cord leads to early up-regulation of cNOS and late up-regulation of iNOS. Different NOS isozymes may play different roles in secondary spinal cord injury.
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