Spondyloarthritis (SpA) correlates with elevated serum secretory-IgA (sSIgA). Retrotranscytosis, mediated by CD71 and Dectin-1, is linked to high sSIgA levels. This research investigated retrotranscytosis in the gut o...
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Immunosenescence triggers increased morbidity in geriatrics; therefore, therapeutic intervention is required. This study aims to find out the effect of mango mistletoes/Dendrophthoe pentandra (MM/Dp) towards CD4/CD8 r...
Immunosenescence triggers increased morbidity in geriatrics; therefore, therapeutic intervention is required. This study aims to find out the effect of mango mistletoes/Dendrophthoe pentandra (MM/Dp) towards CD4/CD8 ratio, CD4+CD28- percentage, CD8+CD28− percentage, and IL-2 level on old female mice. The mango mistletoes leaves were extracted using ethanol 96%, and then administered orally once a day for 30 days (150, 300, and 600 mg/kgBW). CD4/CD8 ratio, and percentage of CD4+CD28- and CD8+CD28− were examined using flowcytometry on the spleen sample. ELISA was performed on the blood sample extracted from the heart to study the IL-2 level. MM/Dp could lower the percentage of CD4+CD28- significantly (p=0.001) (300 and 600 mg/kgBW), but statistically insignificant (p=0.121) in reducing the percentage of CD8+CD28- (but has positive trend). Although MM/Dp was insignificant in increasing the IL-2 level (p=0.124), there was an increase in trend at a dose of 600 mg/kgBW (2.53 pg/ml) compared to the control group (2.02 pg/ml). The administration of various doses had a strong correlation (r=0.496) with the decreasing CD4/CD8 ratio. MM/Dp has potentials in inhibiting immunosenescence that is signified by the decreasing percentage of CD4+CD28− and CD8+CD28-, as well as increasing the IL-2 level.
Molecular docking is a bioinformatics tool used to study and analyze ligand-receptor interactions. Centella asiatica (CA) is known as an herbal medicine for many diseases. Madecassic acid, madecassoside, asiatic acid,...
Molecular docking is a bioinformatics tool used to study and analyze ligand-receptor interactions. Centella asiatica (CA) is known as an herbal medicine for many diseases. Madecassic acid, madecassoside, asiatic acid, and asiaticoside are compounded from CA potentially as antiaging and wound healing agents. This study aimed to evaluate the potential of CA for anti-aging and wound healing through molecular docking. The docking was carried out on proteins associated with the antioxidant, collagen degradation pathway, and inflammatory processes. The molecular docking study used Glide, ADME/T using QikProp, and binding free energy calculations employed Prime/MM-GBSA module and Schrodinger suite 2020-2. The molecular docking and MM-GBSA showed specific interactions and high affinity within the binding pocket of the ligand. The in silico ADME/T properties were predicted that the compounds may follow the criteria for oral and topical active drugs. The theoretical calculation derived from ADME/T and molecular docking provided in-depth information into the structural basis, specific bonding, and nonbonding interactions governing the inhibition of antioxidant, collagen degradation pathway, and inflammatory processes. Taken together, these findings provide a basis for the recommendation of compounds from CA as high-affinity ligands and drug candidates for anti-aging and wound healing.
Stunting is a growing problem caused by chronic inadequacy of nutrition intake and chronic inflammation, mainly during the first 1000 days of life. This study aims to evaluate the incidence of stunting in terms of non...
Stunting is a growing problem caused by chronic inadequacy of nutrition intake and chronic inflammation, mainly during the first 1000 days of life. This study aims to evaluate the incidence of stunting in terms of non-exclusive breastfeeding, infectious diseases, and sanitation in Pujon subdistrict, Malang, East Java, Indonesia. This study was an observational analytic study with the case-control design carried out in 10th - 21st March 2020 in Pujon subdistrict, Malang district, East Java. This study involved 76 pairs of mothers and children age 24 - 59 months (39 cases and 37 control samples). Data were collected by questionnaire, while the statistical analysis used Chi-square test followed by the analysis of odds ratio (OR) with 95% CI. The results show that the risk factors for stunting in toddlers due to a non-exclusive history of breastfeeding (OR = 2.995), history of infectious diseases such as fever (OR = 3.059), Acute Respiratory Infection (OR = 4.452), diarrhea (OR = 7.083), and waste disposal facilities (OR = 4.000) sewage water disposal facilities (OR = 5.739). It can be concluded that non-exclusive breastfeeding, infection disease, and sanitation such as waste and sewage water disposal facilities are risk factors for stunting children aged 24 - 59 months in Pujon subdistrict, Malang, East Java, Indonesia.
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