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Exploring the high-temperature tensile behavior of additively manufactured 316L stainless steel with fine equiaxed grains

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Journal of Materials Research and technology 2025年 36卷 9790-9802页

作者： Jong-Soo Bae Kwang-Hyeok Lim Shubham Chandra Xipeng Tan Gi-dong Sim Department of Mechanical Engineering Korea Advanced Institute of Science and Technology 291 Daehak-ro Yuseong-gu Daejeon 34141 Republic of Korea Agency for Defense Development 160 Bugyuseong-daero 488 beon-gil Yuseong-gu Daejeon 34060 Republic of Korea Singapore Centre for 3D Printing School of Mechanical and Aerospace Engineering Nanyang Technological University 639798 Singapore Department of Mechanical Engineering National University of Singapore 9 Engineering Drive 1 Singapore 117575 Singapore Department of Materials Science and Engineering National University of Singapore 9 Engineering Drive 1 Singapore 117575 Singapore

Powder-bed fusion additive manufacturing (PBF-AM) technology has attracted substantial attention as an innovative method for rapidly and accurately fabricating complex, near-net-shape metallic components. While stainless steels produced via PBF-AM generally exhibit excellent mechanical properties at room temperature—due to the unique microstructural characteristics formed under steep thermal gradients and rapid cooling rates—their behavior at elevated temperatures remains underexplored. Here, a comprehensive study is conducted on the mechanical behavior of a fine equiaxed-grained 316L stainless steel (SS) additively manufactured by the electron-beam powder bed fusion (EB-PBF) method. Over temperatures ranging from room temperature to 600 °C, EB-PBF 316L SS exhibits isotropic behavior and remarkable tensile properties (yield strength, ultimate tensile strength, and elongation) compared to conventional wrought 316L SS. Grain boundary strengthening and dispersion strengthening from amorphous silicon oxide nanoparticles collectively account for the reasonably good room-temperature yield strength, and these particles continue to impede dislocation motion at elevated temperatures. The temperature-dependent mechanical properties of EB-PBF 316L SS are examined in the context of evolving deformation mechanisms and microstructural characteristics. Above 200 °C, deformation twinning is suppressed, whereas dynamic strain aging (dSA) arises between 300 °C and 600 °C. despite the presence of dSA, no abrupt drop in ductility is observed, which is attributed to the formation of sub-grains in the strain-free state through dynamic recrystallization during plastic deformation.

关键词： High-temperature tensile properties Electron-beam powder bed fusion 316L stainless steel Fine equiaxed-grain Additive manufacturing

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Interfacial Microstructures and Mechanical Properties of TiC Reinforced GH 3230 Superalloy Manufactured by Laser Metal deposition

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Chinese Journal of Mechanical Engineering 2024年第6期37卷 43-58页

作者： Yan Wang Neng Li Wei Liu Chonglin Jia Yu Wu Chao Gao Huaping Xiong Division of Welding and Plastic Forming 3D Printing Research and Engineering Technology CenterBeijing Institute of Aeronautical MaterialsBeijing 100095China Science and Technology on Advanced High Temperature Structural Materials Laboratory Beijing Institute of Aeronautical MaterialsBeijing 100095China

GH 3230 superalloy is a solution strengthening nickel-based superalloy and it is commonly used for fabricating hot components with the service temperature of above 900℃.In order to further improve high-temperature performance,nickel-based alloy matrix composites(NMCs)were ***,it is known that laser additive manufacturing is an optional method for fabricating nickel-based ***,the research on ceramic-reinforced GH 3230 fabricated by laser metal deposition(LMd)are highly *** aim of this study is to develop TiC ceramic particle reinforced GH 3230 composites using laser metal deposition(LMd)method and study the effect of TiC content on their microstructure and tensile *** results showed that TiC particles not only changed the intensity and position of the X-ray diffraction peaks of the alloy matrix but also had a significant effect on the refinement of the cellular ***,it was found that an interfacial layer with sub-micrometer thickness was formed between the TiC ceramic particle and the superalloy matrix,which was identified to be(W,Ti)C_(1-x)phase by the *** terms of the as-built composites,the ultimate tensile strength(UTS)and yield strength(YS)gradually increased,but elongation(EL)decreased with the increase of TiC *** the as-LMded 10 vol.%TiC/GH3230 composites,UTS and EL reached 1077.0 MPa and 12.4%,*** enhancement of the tensile strength for composites was attributed to the combined effect of grain refinement strengthening,Orowan strengthening,dislocation strengthening and loading-bearing strengthening.

关键词： Laser metal deposition GH 3230 superalloy TiC particles Mechanical properties Interfacial layer

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Magnesium alloy implant GZ31K with enhanced corrosion resistance promotes fracture healing in vitro and in vivo systems

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Journal of Magnesium and Alloys 2025年

作者： Yusufu, Yalikun Zhang, Yunyang Liu, Xinyun Jiang, Jiahao Shao, Zhiqiang Sun, Minghui Zhang, Xiaobo Jiang, Qing Division of Sports Medicine and Adult Reconstructive Surgery Department of Orthopedic Surgery Nanjing Drum Tower Hospital Affiliated Hospital of Medical School Nanjing University Jiangsu Nanjing 210008 China State Key Laboratory of Pharmaceutical Biotechnology Nanjing University Jiangsu Nanjing 210008 China Branch of National Clinical Research Center for Orthopedics Sports Medicine and Rehabilitation Jiangsu Nanjing 210008 China Institue of Medical 3D Printing Nanjing University Jiangsu Nanjing 210008 China Jiangsu Engineering Research Center for 3D Bioprinting Jiangsu Nanjing 210008 China Centre for Shared Scientific Research Facilities Nanjing University Jiangsu Nanjing 210023 China School of Stomatology Xuzhou Medical University Jiangsu Xuzhou 221004 China Jiangsu Key Laboratory of Advanced Structural Materials and Application Technology School of Materials Science and Engineering Nanjing Institute of Technology Jiangsu Nanjing 211167 China

Magnesium and its alloys have garnered significant attention as promising materials for bone tissue engineering, owing to their bone-like density and elastic modulus, favorable mechanical properties, biodegradability, biocompatibility, and diverse biological activities. However, rapid degradation, subcutaneous gas formation from H₂ release, and osteolysis caused by elevated Mg concentrations have limited its widespread clinical application. In this study, Mg-3.0Gd-1Zn-0.4Zr (GZ31K) alloy with desirable uniform degradation and stress corrosion resistance under extruded and drawn condition was used as internal fixation implants for fracture healing, while the commercially available WE43 alloy was used as control. Results revealed that GZ31K alloy exhibited refined grain structure, nanoscale distributed stacking faults and superior corrosion resistance compared to WE43 alloy. The corrosion rate of the extruded GZ31K and WE43 alloys are 0.25 mm/year and 0.35 mm/year, meeting the corrosion tolerance threshold for orthopedic implants (<0.5 mm/year). In vitro study demonstrated that GZ31K alloy exhibited pronounced biocompatibilities and osteogenic bioactivities towards rat bone marrow mesenchymal stem cells (rBMSCs) compared with WE43 alloy, as evidenced by the enhanced ALP activity level, mineralized nodule formation and expression of osteogenic-related marker genes. In vivo results confirmed that GZ31K alloy retained its above 87.1% structural integrity for up to 8 weeks post-implantation and exhibited better corrosion resistance compared to the WE43 alloy (80.9%). Besides, the Sprague-dawley rats administrated with GZ31K alloy exhibited greater bone volume, trabecular thickness, satisfactory load-bearing performance and surface degradation behavior at 8 weeks post-fracture healing compared to the Kirschner wire and the WE43 alloy. Taken together, these findings highlighted that GZ31K alloy with slower degradation rate, enhanced structural stability, exceptio

关键词： Bioactive material Biodegradable magnesium alloy degradation Orthopedic implant Osteoporotic fracture healing

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Recent research of peptide-based hydrogel in nervous regeneration

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Bioactive Materials 2024年第10期40卷 503-523页

作者： Chunmei Xie Yueyang Chen Lang Wang Kin Liao Bin Xue Yulong Han Lan Li Qing Jiang State Key Laboratory of Mechanics and Control of Mechanical Structures Nanjing University of Aeronautics and AstronauticsNanjing210016China Advanced Digital and Additive Manufacturing Center Khalifa University of Science and TechnologyPo Box 127788Abu DhabiUnited Arab Emirates National Laboratory of Solid State Microstructures Department of PhysicsNanjing UniversityNanjingChina State Key Laboratory of Pharmaceutical Biotechnology Division of Sports Medicine and Adult Reconstructive SurgeryDepartment of Orthopedic SurgeryDrum Tower Hospital Affiliated to Medical School of Nanjing UniversityNanjingChina Jiangsu Engineering Research Center for 3D Bioprinting NanjingChina Institute of Medical 3D Printing Nanjing UniversityNanjingChina

Neurological disorders exert significantly affect the quality of life for patients,necessitating effective strategies for nerve *** traditional autologous nerve transplantation and emerging therapeutic approaches encounter scientific challenges due to the complex nature of the nervous system and the unsuitability of the surrounding environment for cell *** engineering techniques offer a promising path for *** neural tissue engineering relies on modulating cell differentiation behavior and tissue repair by developing biomaterials that mimic the natural extracellular matrix(ECM)and establish a threedimensional ***-based hydrogels have emerged as a potent option among these biomaterials due to their ability to replicate the structure and complexity of the *** review aims to explore the diverse range of peptide-based hydrogels used in nerve regeneration with a specific focus on dipeptide hydrogels,tripeptide hydrogels,oligopeptide hydrogels,multidomain peptides(MdPs),and amphiphilic peptide hydrogels(PAs).Peptide-based hydrogels offer numerous advantages,including biocompatibility,structural diversity,adjustable mechanical properties,and degradation without adverse ***,hydrogels formed from self-assembled polypeptide nanofibers,derived from amino acids,show promising potential in engineering neural tissues,outperforming conventional materials like alginate,poly(ε-caprolactone),and ***,the simple design and cost-effectiveness of dipeptidebased hydrogels have enabled the creation of various functional supramolecular structures,with significant implications for nervous system *** hydrogels are expected to play a crucial role in future neural tissue engineering *** review aims to highlight the benefits and potential applications of peptidebased hydrogels,contributing to the advancement of neural tissue engineering.

关键词： Self-assembled dipeptide Cyclic dipeptide Hydrogels Nervous regeneration

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Author Correction: Bioprinting of bespoke islet-specific niches to promote maturation of stem cell-derived islets

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Nature communications 2025年第1期16卷 2205页

作者： Myungji Kim Seungyeun Cho dong Gyu Hwang In Kyong Shim Song Cheol Kim Jiwon Jang Jinah Jang Division of Interdisciplinary Bioscience and Bioengineering Pohang University of Science and Technology (POSTECH) Pohang Republic of Korea. Center for 3D Organ Printing and Stem Cells Pohang University of Science and Technology (POSTECH) Pohang Republic of Korea. Asan Institute for Life Science University of Ulsan College of Medicine and Asan Medical Center Seoul Republic of Korea. Division of Hepato-Biliary and Pancreatic Surgery Department of Surgery University of Ulsan College of Medicine and Asan Medical Center Seoul Republic of Korea. Department of Life Sciences Pohang University of Science and Technology (POSTECH) Pohang Republic of Korea. Division of Interdisciplinary Bioscience and Bioengineering Pohang University of Science and Technology (POSTECH) Pohang Republic of Korea. Jinahjang@postech.ac.kr. Center for 3D Organ Printing and Stem Cells Pohang University of Science and Technology (POSTECH) Pohang Republic of Korea. Jinahjang@postech.ac.kr. Department of Life Sciences Pohang University of Science and Technology (POSTECH) Pohang Republic of Korea. Jinahjang@postech.ac.kr. Department of Mechanical Engineering Pohang University of Science and Technology (POSTECH) Pohang Republic of Korea. Jinahjang@postech.ac.kr. Department of Convergence IT Engineering Pohang University of Science and Technology (POSTECH) Pohang Republic of Korea. Jinahjang@postech.ac.kr. Institute for Convergence Research and Education in Advanced Technology Yonsei University Seoul Republic of Korea. Jinahjang@postech.ac.kr.

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Process Windows of Ni625 Alloy Fabricated Using a direct Energy deposition-Based Lasermeister System

SSRN

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SSRN 2024年

作者： Ekubaru, Yusufu Takuya, Nakabayashi Fujiwara, Tomoharu Poorganji, Behrang Development Department Advanced Manufacturing Business Unit Nikon Corporation 201-9 Miizugahara Saitama Kumagaya360-8559 Japan Advanced Technology Research & Development Division Nikon Corporation 471 Nagaodai-cho Sakae-ku Kanagawa Yokohama-city244-8533 Japan Morf 3D Inc. CA90808 United States

Herein, we developed a process window for Ni625 alloy fabricated using a Nikon Lasermeister laser powder direct energy deposition unit. The process map illustrates the relationship between the laser power, scan speed, and effective energy density, established by examining the correlation between the microstructure and mechanical properties. All samples exhibited a bimodal microstructure comprising equiaxed and columnar dendrite grains, and the dendrite arm spacing decreased with increasing scan speed. The tensile behavior of each sample demonstrated minimal variation, and the values were comparable to those reported previously. The ultimate tensile and yield strengths ranged from 1008 ± 2 to 941 ± 9 and 682 ± 11 to 640 ± 7 MPa, respectively. This study highlights the remarkable manufacturability of Ni625 alloy for additive manufacturing across diverse parameter sets, demonstrating that a single ideal process set does not exist for each material and machine. Instead, multiple "recipes" may be employed to achieve similar outcomes. © 2024, The Authors. All rights reserved.

关键词： 3d printing

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Wireless,multimodal sensors for continuous measurement of pressure,temperature,and hydration of patients in wheelchair

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npj Flexible Electronics 2023年第1期7卷 479-493页

作者： Seokjoo Cho Hyeonseok Han Hyunwoo Park Sung-Uk Lee Jae-Hwan Kim Sung Woo Jeon Mengqiu Wang Raudel Avila Zhaoqian Xi Kabseok Ko Minsu Park Jungyup Lee Myungwoo Choi Je-Sang Lee Weon Gi Min Byeong-Ju Lee Soyeong Lee Jungrak Choi Jimin Gu Jaeho Park Min Seong Kim Junseong Ahn Osman Gul Chankyu Han Gihun Lee Seunghwan Kim Kyuyoung Kim Jeonghyun Kim Chang-Mo Kang Jahyun Koo Sung Soo Kwak Sungbong Kim dong Yun Choi Seokwoo Jeon Hyung Jin Sung Yong Bae Park Minkyu Je Young Tae Cho Yong Suk Oh Inkyu Park Department of Mechanical Engineering Korea Advanced Institute of Science and TechnologyDaejeon 34141Republic of Korea School of Electrical Engineering Korea Advanced Institute of Science and TechnologyDaejeon 34141Republic of Korea Advanced 3D Printing Technology Development Division Korea Atomic Energy Research InstituteDaejeon 34057Republic of Korea Department of Electrical and Computer Engineering University of Illinois at Urbana-ChampaignUrbanaIL 61801USA State Key Laboratory of Structural Analysis for Industrial Equipment Department of Engineering MechanicsDalian University of TechnologyDalian 116023P.R.China Department of Mechanical Engineering Northwestern UniversityEvanstonIL 60208USA DUT-BSU Joint Institute Dalian University of TechnologyDalian 116023China Ningbo Institute of Dalian University of Technology Ningbo 315016China Department of Electronics Engineering Kangwon National UniversityChuncheon 24341Republic of Korea Querrey-Simpson Institute for Bioelectronics Northwestern UniversityEvanstonIL 60208USA Sibel Health Inc NilesIL 60714USA Department of Materials Science and Engineering KAIST Institute for The Nanocentury(KINC)Korea Advanced Institute of Science and TechnologyDaejeon 34141Republic of Korea Department of Rehabilitation Medicine Gimhae Hansol Rehabilitation&Convalescent HospitalGimhae 50924Republic of Korea Department of planning and development Gimhae Hansol Rehabilitation&Convalescent HospitalGimhae 50924Republic of Korea Department of rehabilitation medicine Pusan national university hospitalBusan 49241Republic of Korea Department of AI Convergence Network Ajou UniversitySuwon 16499Republic of Korea Department of Electronic Convergence Engineering Kwangwoon UniversitySeoul 01897Republic of Korea Korea Photonics Technology Institute Gwangju 61007Republic of Korea School of Biomedical Engineering Korea UniversitySeoul 02841Republic of Korea Interdisciplinary Program in Precision Public Health Korea UniversitySeoul 02841Republic of Korea Center

Individuals who are unable to walk independently spend most of the day in a *** population is at high risk for developing pressure injuries caused by ***,early diagnosis and prevention of these injuries still remain ***,we introduce battery-free,wireless,multimodal sensors and a movable system for continuous measurement of pressure,temperature,and hydration at skin *** device design includes a crack-activated pressure sensor with nanoscale encapsulations for enhanced sensitivity,a temperature sensor for measuring skin temperature,and a galvanic skin response sensor for measuring skin hydration *** movable system enables power harvesting,and data communication to multiple wireless devices mounted at skin-cushion interfaces of wheelchair users over full body *** evaluations and numerical simulations of the devices,together with clinical trials for wheelchair patients,demonstrate the feasibility and stability of the sensor system for preventing pressure injuries caused by sitting.

关键词： injuries modal mounted

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Achilles tendinopathy treatment via circadian rhythm regulation

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Journal of advanced Research 2024年

作者： Zhang, Yibo Wu, Yizhang Wang, Yong Lu, Jun Lu, Yang Wang, Peng Li, Lan Yan, Wenjin Cai, Hongling Hannah Leigh, Weisbecker Zhang, Lin Bai, Wubin Jiang, Qing Xu, Xingquan State Key Laboratory of Pharmaceutical Biotechnology Division of Sports Medicine and Adult Reconstructive Surgery Department of Orthopedic Surgery Nanjing Drum Tower Hospital Affiliated Hospital of Medical School Nanjing University Nanjing 210008 China Department of Applied Physical Sciences University of North Carolina at Chapel Hill Chapel Hill 27599 NC United States Wide Bandgap Semiconductor Technology Disciplines State Key Laboratory School of Microelectronics Academy of Advanced Interdisciplinary Research Xidian University Xi'an 710071 China Jiangsu Key Laboratory for Biomaterials and Devices State Key of Laboratory Bioeletronics School of Biological Sciences & Medical Engineering Southeast University Nanjing 210096 China Jiangsu Engineering Research Center for 3D Bioprinting Nanjing 210000 China National Laboratory of Solid State Microstructures Collaborative Innovation Center of Advanced Microstructures School of Physics Nanjing University Nanjing 210093 China Institute of Medical 3D Printing Nanjing University Nanjing 210000 China

Introduction: Achilles tendinopathy (AT) is a prevalent musculoskeletal disorder closely linked to oxidative stress. Existing evidence suggests a potential link between circadian clock rhythms and oxidative stress. However, the precise role of the circadian clock in the progression and treatment of AT remains unclear. Objective: The purpose of this study was to investigate the role of the Achilles tendon circadian clock in AT pathology and explore the potential use of biomaterials for modulating the circadian clock in the treatment of AT. Methods: We utilized in vivo and in vitro models to investigate the alterations of the circadian clock within the Achilles tendon during the progression of AT, as well as its impact on disease development. Additionally, we fabricated Nb₂C@CeO₂ composites featuring a Schottky heterojunction for regulating the circadian rhythm and validated its therapeutic efficacy and molecular mechanism of AT through both in vivo and in vitro experiments. Results: The Achilles tendon functioned as a peripheral oscillator with an independent and self-sustained time-keeping system. The rhythm of the Achilles tendon clock was disrupted during the development of AT, as indicated by the decreased amplitude of Bmal1 and Nrf2 rhythm expression. Mechanistically, the knockdown of Bmal1 disrupted the Achilles tendon clock, thereby destroying the Bmal1-Nrf2 axis dependent molecular defense mechanism, and exacerbating the inflammatory response, whereas overexpression of Bmal1 had a protective effect. Nb₂C@CeO₂ composites with Schottky heterojunctions enhance intercellular electrical signaling, boosting Bmal1 expression and mitigating AT's pathological changes. Importantly, enhancing Bmal1 expression during its peak, rather than its trough, was more effective. Conclusion: This study identified the protective role of the circadian clock against oxidative stress and inflammation in the Achilles tendon. Achilles tendon c

关键词： Achilles tendinopathy Bmal1-Nrf2 axis Circadian clock Schottky heterojunction Time of administration

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PARALLEL ANd dISTRIBUTEd TLM COMPUTATION WITH SIGNAL-PROCESSING FOR ELECTROMAGNETIC-FIELd MOdELING

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INTERNATIONAL JOURNAL OF NUMERICAL MOdELLING-ELECTRONIC NETWORKS dEVICES ANd FIELdS 1995年第3-4期8卷 169-185页

作者： SO, PPM ESWARAPPA, C HOEFER, WJR NSERC/MPR Teltech Research Chair in RF Engineering Department of Electrical and Computer Engineering University of Victoria Victoria British Columbia V8W 3P6 Canada Poman So received his B.Sc. degree in computer science and physics from the University of Toronto Toronto Onatario Canada in 1985. He obtained his B.A. Sc. and M.A.Sc. degrees in electrical engineering (summa cum laude) from the University of Ottawa Ottawa Ontario Canada in 1987 and 1989 respectively. Mr So was a research engineer in the University of Ottawa from January 1989 to October 1991 his research interests included CAD techniques of microwave circuits and numerical methods for electromagnetic wave modelling. He specialized in the development of electromagnetic engineering CAD software and successfully implemented a number of electromagnetic wave simulators based on the two-dimensional and three-dimensional transmission-line matrix methods. From August 1990 to February 1991 he accompanied Professor W. J. R. Hoefer to Rome Italy and Sophia Antiopolas France. During that time he implemented a parallel version of the 3D-TLM simulator for the CM-2 Connection Machine using C-Star and X Windows Library. He is a research engineer and a Ph.D. student at the University of Victoria Victoria British Columbia Canada. He ported the 2D- and 3D-TLM simulators to DECmpp 1200 massively parallel computer using MPL C and C++. He also combined OSA90/hope a commercially available microwave CAD program with a 2D-TLM simulation module for geometry optimization using the Datapipe technique of OSA90/hope. Recently he has developed a distributed client-server computing technique for the TLM method this technique could accelerate the TLM simulation by more than an order of magnitude. Channabasappa Eswarappa received the M.Tech. degree in electrical engineering from the Indian Institute of Technology Kanpur India in 1983 and Ph. D. degree from the University of Ottawa Canada in 1990. He worked as an Assistant Executive Engineer and later as

This paper describes the implementation of transmission-line matrix (TLM) method algorithms on a massively parallel computer (dECmpp 12000), the technique of distributed computing in the UNIX environment, and the combination of TLM analysis with Prony's method as well as with autoregressive moving average (ARMA) digital signal processing for electromagnetic field modelling. By combining these advanced computation techniques, typical electromagnetic field modelling of microwave structures by TLM analysis can be accelerated by a few orders of magnitude.

关键词： Mathematical models

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3d extrusion bioprinting

NATURE REVIEWS METHODS PRIMERS

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NATURE REVIEWS METHOdS PRIMERS 2021年第1期1卷 1页

作者： Willson, Joseph Division of Engineering in Medicine Brigham and Women’s Hospital Department of Medicine Harvard Medical School Cambridge MA USA Department of Mechanical Engineering University of Minnesota Minneapolis MN USA Laboratory of Stem Cell Bioengineering Institute of Bioengineering School of Life Sciences and School of Engineering Ecole Polytechnique Fédérale de Lausanne (EPFL) Lausanne Switzerland Institute of Chemical Sciences and Engineering School of Basic Science EPFL Lausanne Switzerland Trinity Centre for Biomedical Engineering Trinity Biomedical Sciences Institute Trinity College Dublin Dublin Ireland Department of Mechanical Manufacturing and Biomedical Engineering School of Engineering Trinity College Dublin Dublin Ireland Advanced Materials and Bioengineering Research Centre (AMBER) Royal College of Surgeons in Ireland and Trinity College Dublin Dublin Ireland Department of Anatomy and Regenerative Medicine Royal College of Surgeons in Ireland Dublin Ireland School of Mechanical and Aerospace Engineering Nanyang Technological University Singapore Singapore Singapore Centre for 3D Printing (SC3DP) School of Mechanical and Aerospace Engineering Nanyang Technological University Singapore Singapore HP-NTU Digital Manufacturing Corporate Lab Nanyang Technological University Singapore Singapore Department of Health Sciences and Technology ETH Zürich Zürich Switzerland Department of Orthopedics University Medical Center Utrecht Utrecht Netherlands Department of Clinical Sciences Faculty of Veterinary Medicine Utrecht University Utrecht Netherlands

This PrimeView on 3d extrusion bioprinting accompanies the Primer by Zhang et al. and highlights the main stages of the 3d extrusion bioprinting process.

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