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Increase of xylitol production rate by controlling redox potential inCandida parapsilosis

Biotechnology and Bioengineering 2000年第4期58卷

作者： deok‐Kun Oh Sang‐Yong Kim Jung‐Hoe Kim Department of Food Science and Technology Woosuk University Cheonju 565‐800 Korea Tong Yang Confectionery Co. R&D Center Seoul 140‐715 Korea Department of Biological Sciences Korea Advanced Institute of Science and Technology Taejon 305‐701 Korea

The effect of redox potential on xylitol production by Candida parapsilosis was investigated. The redox potential was found to be useful for monitoring the dissolved oxygen (dO) level in culture media, especially when the dO level was low. An increase in the agitation speed in a 5 L fermentor resulted in an increased culture redox potential as well as enhanced cell growth. Production of xylitol was maximized at a redox potential of 100 mV. As the initial cell concentration increased from 8 g/L to 30 g/L, the volumetric productivity of xylitol increased from 1.38 g/L · h to 4.62 g/L · h. A two‐stage xylitol production strategy was devised, with stage 1 involving rapid production of cells under well‐aerated conditions, and stage 2 involving cultivation with reduced aeration such that the culture redox potential was 100 mV. Using this technique, a final xylitol concentration of 180 g/L was obtained from a culture medium totally containing 254.5 g/L xylose in a 3,000 L pilot scale fermentor after 77 h fermentation. The volumetric productivity of xylitol during the fermentation was 2.34 g/L · h. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 58:440‐444, 1998.

关键词： xylitol production redox potential dissolved oxygen Candida parapsilosis

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Mass production of thermostable d‐hydantoinase by batch culture of recombinantEscherichia coliwith a constitutive expression system

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Biotechnology and Bioengineering 2000年第4期56卷

作者： dong‐Cheol Lee Geun‐Joong Kim Yoo‐Kyong Cha Cheo‐Young Lee Hak‐Sung Kim Department of Biological Sciences Korea Advanced Institute of Science and Technology 373‐1 Kusong‐Dong Yusong‐Gu Taejon 305‐701 Korea telephone: 82‐42‐869‐2616 fax: 82‐42‐869‐2610 R&D Section Tong‐Suh Petrochemical Corp. LTD. 154 Bukok‐Dong Nam‐Gu Ulsan 680‐110 Korea

d ‐Hydantoinase is an industrial enzyme widely used for the synthesis of optically active d ‐amino acids. A gene encoding thermostable d ‐hydantoinase of Bacillus stearothermophilus Sd‐1 has previously been cloned and constitutively expressed by its native promoter in Escherichia coli XL1‐Blue (Lee et al., 1996b). In this work, we attempted mass production of the d ‐hydantoinase by batch culture of the recombinant E. coli using glycerol as a carbon source. The plasmid content in cells increased in proportion to the culture temperature, which resulted in a two‐ or three‐fold increase of the specific d ‐hydantoinase activity at 37°C compared with that at 30°C. The plasmid was stably maintained over 80 generations. When glycerol was initially added to a concentration of 100 g/L, the final biomass concentration reached about 50 g‐dry cell weight/L in a 50 L‐scale fermentation, resulting in the specific enzyme production of 3.8 × 10 4 unit/g‐dry cell weight in a soluble form. Glycerol‐using batch cultivation of recombinant E. coli was found to be a cost‐effective process for the mass production of industrially useful d ‐hydantoinase. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 56: 449–455, 1997.

关键词： thermostable d‐hydantoinase recombinant E. coli constitutive expression glycerol batch cultivation

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Effect of particles on partial discharge inception in oil immersed insulating system

Effect of particles on partial discharge inception in oil im...

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International Conference on Conduction and Breakdown in dielectric Liquids

作者： H. Kurita O. Usui T. Hasegawa H. Fujii Electromechanical System Department Advanced Technology R&D Center Mitsubishi Electric Corporation Limited Amagasaki Hyogo Japan

The behavior of a spherical metallic particle was observed with a high speed camera at the instance of partial discharge inception in an oil immersed insulating system. The polarity of the metallic particle charge was evaluated by the behavior of the particle and the direction of the electric field. A positively charged particle moves to the pressboard located on a negative electrode to initiate the partial discharge. The charge quantity of the particle can be calculated by the behavior of particles and electric fields. The movement of particle is notable in an AC peak voltage so that the partial discharge initiation can be observed on the peak voltage. A mechanism of partial discharge inception is proposed.

关键词： Partial discharges Petroleum Oil insulation Cameras dielectrics and electrical insulation Electrodes Voltage Glass dielectric liquids Partial discharge measurement

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advanced transportation in the dismantling/recycling factory-development of the flexible transfer system

Advanced transportation in the dismantling/recycling factory...

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International Symposium on Environmentally Conscious design and Inverse Manufacturing (Ecodesign)

作者： Y. Inada H. Yamamoto S. Shibata T. Fukuda N. Kubota Technology R&D Center Mechatronics Technology Section Toyo Engineering Corporation Narashino Chiba Japan Center for Cooperative Research in Advanced Science and Technology University of Nagoya Nagoya Japan Department of Mechanical Engineering Osaka Institute of Technology Osaka Japan

To advance the recycling of end-of-life products, the improvement of disassembling work environments such as automations and human-friendly work environments is needed. On the disassembling processes used in dismantling/recycling factories so far however, there have been many uncertainties due to the nonuniformity of the human work and varied conditions of end-of-life products such as degradation, deformation, corrosion and so on. Especially the difficulty of the prescheduling of a disassembling work due to the nonuniform work time (tact-time) impedes the effective operation in the disassembling process in the dismantling/recycling factor? Thus this paper presents the flexible transfer system that is suitable for end-of-life-products disassembling process to provide adjustable transportation management irrespective of the nonuniform work time.

关键词： Transportation recycling Production facilities Manufacturing systems Humans Environmental economics Uncertainty Corrosion Automation research and development

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Experimental investigation of noise immunity diagnosis for printed circuit boards by bulk current injection test

Experimental investigation of noise immunity diagnosis for p...

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IEEE International Symposium on Electromagnetic Compatibility (EMC)

作者： K. Sasabe K. Yoshida A. Bullivant O. Fujiwara Corporate Quality R&D Centre Matsushita Electric Works Limited Kadoma Osaka Japan EMC Advanced Projects Department ERA Technology Limited Leatherhead Surrey UK Department of Electrical and Computer Engineering Nagoya Institute of Technology Nagoya Japan

A simple method for diagnosing noise immunity of printed circuit boards (PCBs) by the bulk current injection (BCI) test was proposed, which can contribute to the PCB trace designs for common-mode noise. A grading index, which is defined as the ratio of the stray capacitances with and without critical IC of malfunction, was introduced to distinguish PCBs susceptible to the common-mode noise. This proposed method was validated experimentally using four PCBs with the same circuit but different trace designs. It was observed that the noise immunity of PCBs had a good correlation with the values of these grading indices.

关键词： Circuit noise Printed circuits Integrated circuit noise Crosstalk Impedance Immunity testing Capacitance Circuit testing Electromagnetic compatibility Circuit synthesis

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Experiments of backward tracking control for trailer system

Experiments of backward tracking control for trailer system

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IEEE International Conference on robotics and Automation (ICrA)

作者： doh-Hyun Kim Jun-Ho Oh 1st Group R&D Laboratory LG Precision Company Limited Suwon Gyeonggi South Korea Department of Mechanical Engineering Korea Advanced Institute of Science and Technology Taejeon South Korea

Trailer systems consist of a steering tractor and a passive trailer linked together with rigid free joint. The backward driving of a trailer system is more difficult than the forward driving, because the backward driving of the trailer system is unstable. We study the backward tracking control for trailer systems and implement the asymptotically stable tracking controller using the Lyapunov direct method. We perform some experiments using a visual feedback system. The experimental results show stable responses and the effectiveness of our method.

关键词： Control systems Mobile robots Kinematics Open loop systems Trajectory Nonlinear control systems radio control Vehicle driving Charge coupled devices Charge-coupled image sensors

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On the use of LSF intermodel interlacing property for spectral quantization

On the use of LSF intermodel interlacing property for spectr...

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IEEE Workshop on Speech Coding Proceedings

作者： Mi Suk Lee Hong Kook Kim Seung Ho Choi Hwang Soo Lee Department of Electrical Engineering Korea Advanced Institute of Science and Technology Daejeon South Korea AT and T Research Laboratories Florham Park NJ USA Human & Computer Interaction Laboratory Samsung Advanced Institute of Technology Gyeonggi South Korea Central R&D center SK Telecom Daejeon South Korea

The line spectral frequencies (LSFs) extracted from successive analysis orders are interlaced with each other. This intermodel interlacing property gives a new relationship between the closeness of LSFs and their spectral sensitivities, which enables us to propose a weighting function for LSF distortion measurement. By applying the proposed weighting function to an LSF quantizer, we can achieve better performance than when using the conventional heuristic functions. Moreover, the complexity of the proposed weighting function is much lower than that of the optimal weighting function, while their performances are almost the same.

关键词： Quantization Frequency Covariance matrix distortion measurement Linear predictive coding Euclidean distance Weight measurement Polynomials reflection Humans

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Intelligent Monitoring System for Grinding Process by recurrent Fuzzy Inference -Evaluation of Inferred Surface roughness Using degree of Fuzziprsss-

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Journal of robotics and Mechatronics 1999年第5期11卷 423-430页

作者： Kobayashi, Futoshi Arai, Fumihilo Fukuda, Toshio Onoda, Makoto Hottav, Yuzo Department of Micro System Engineering Nagoya University 1 Furo-cho Chikusa-ku Nagoya464-8603 Japan Center for Cooperative Research in Advanced Science & Technology Nagova University 1 Furo-cho Chikusa-ku Nagoya464-8601 Japan Production Machinery R&D Center NTN Corporation 1578 Higashi-Kaizuka Iwata438-8510 Japan

Grinding process is frequently used to produce a smooth surface in the manufacturing system. recently, for using a super-abrasive wheel economically, grinding system needs to measure the surface roughness. However, it is difficult to measure surface roughness in process because it takes a long time to measure it. We have to infer the surface roughness in process by online sensor information and evaluate the reliability of inferred surface roughness. We have proposed recurrent Fuzzy Inference (rFI) for estimating it. But, rFI cannot evaluate the reliability of inferred surface roughness. In this paper, we evaluate the reliability of inferred surface roughness by the degree of fuzziness that shows the lack of difference between the fuzzy set and the fuzzy complement. We show the experimental result of inferring, and evaluating the surface roughness in grinding process. © 1999, Fuji technology Press. All rights reserved.

关键词： Surface roughness

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MILLIPEdE: Easy Parallel Programming in Available distributed Environments

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Software: Practice and Experience 1999年第8期27卷

作者： rOY FrIEdMAN MAXIM GOLdIN AYAL ITZKOVITZ ASSAF SCHUSTEr Department of Computer Science Cornell University Ithaca NY 14850 USA Intel R&D Center Israel Center of Advanced Technologies Haifa Israel. Department of Computer Science The Technion – Israel Institute of Technology Haifa 32000 Israel.

MILLIPEdE is a project aimed at developing a distributed shared memory environment for parallel programming. A major goal of this project is to support easy-to-grasp parallel programming languages that will also make it straightforward to parallelize existing code. Other targets are forward compatibility and availability of both the user programs (hence the shared memory support and the C-like parallel language PArC) and the system itself (which is thus implemented in user-level and using the operating system exported services). Locality of memory references, which implies efficiency and speedups, is maintained by MILLIPEdE} using page and thread migration, through which dynamic load-balancing and weak memory are implemented. ©1997 by John Wiley & Sons, Ltd.

关键词： parallel programming distributed computing distributed shared memory weak memory

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Contributory presentations/posters

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Journal of Biosciences 1999年第1期24卷 33-198页

作者： N. Manoj V. r. Srinivas A. Surolia M. Vijayan K. Suguna r. ravishankar r. Schwarzenbacher K. Zeth diederichs G. M. Kostner A. Gries P. Laggner r. Prassl Madhusudan Pearl Akamine Nguyen-huu Xuong Susan S. Taylor M. Bidva Sagar K. Saikrishnan S. roy K. Purnapatre P. Handa U. Varshney B. K. Biswal N. Sukumar J. K. Mohana rao A. Johnson Vasantha Pattabhi S. Sri Krishna Mira Sastri H. S. Savithri M. r. N. Murthy Bindu Pillai Kannan M. V. Hosur Mukesh Kumar Swati Patwardhan K. K. Kannan B. Padmanabhaa S. Sasaki-Sugio M. Nukaga T. Matsuzaki S. Karthikevan S. Sharma A. K. Sharma M. Paramasivam P. Kumar J. A. Khan S. Yadav A. Srinivasan T. P. Singh S. Gourinath Neelima Alam A. Srintvasan Vikas Chandra Punit Kaur Ch. Betzel S. Ghosh A. K. Bera S. Bhattacharya S. Chakraborty A. K. Pal B. P. Mukhopadhyay I. dey U. Haldar Asok Baneriee Jozef Sevcik Adriana Solovicova K. Sekar M. Sundaralingam N. Genov dong-cai Liang Tao Jiang Ji-ping Zhang Wen-rui Chang Wolfgang Jahnke Marcel Blommers S. C. Panchal r. V. Hosur Bindu Pillay Puniti Mathur S. Srivatsun ratan Mani Joshi N. r. Jaganathan V. S. Chauhan H. S. Atreya S. C. Sahu K. V. r. Chary Girjesh Govil Elisabeth Adjadj Éric Quinjou Nadia Izadi-Pruneyre Yves Blouquit Joël Mispelter Bernadette Heyd Guilhem Lerat Philippe Milnard Michel desmadreil Y. Lin B. d. Nageswara rao Vidva raghunathan Mei H. Chau Prashant Pesais Sudha Srivastava Evans Coutinho Anil Saran Leizl F. Sapico Jayson Gesme Herbert Lijima raymond Paxton Thamarapu Srikrishnan C. r. Grace G. Nagenagowda A. M. Lynn Sudha M. Cowsik Sarata C. Sahu S. Chauhan A. Bhattacharya G. Govil Anil Kumar Maurizio Pellecchia Erik r. P. Zuiderweg Keiichi Kawano Tomoyasu Aizawa Naoki Fujitani Yoichi Hayakawa Atsushi Ohnishi Tadayasu Ohkubo Yasuhiro Kumaki Kunio Hikichi Katsutoshi Nitta V. rani Parvathy r. M. Kini Takumi Koshiba Yoshihiro Kobashigawa Min Yao Makoto demura Astushi Nakagawa Isao Tanaka Kunihiro Kuwajima Jens Linge Seán O. donoghue Michael Nilges G. Chakshusmathi Girish S. ratnaparkhi P. K. Madhu r. Varadarajan C. Tetreau Molecular Biophysics Unit Indian Institute of Science Bangalore India Austrian Academy of Sciences Institute of Biophysics and X-ray Structure Research Graz Austria Faculty of Biology University of Konstanz Germany Institute of Medical Biochemistry University of Graz Austria Howard Huges Medical Institute Department of Chemistry & Biochemistry University of California La Jolla USA Department of Chemistry & Biochemistry University of California San Diego La Jolla USA Molecular Biophysics Unit India Department of Microbiology &Cell Biology Indian Institute of Science Bangalore India Macromolecular Structure Laboratory NCI - Frederick Cancer Research and Development Center ABL - Basic Research Program Frederick USA Department of Crystallography and Biophysics University of Madras Chennai Biochemistry Department Indian Institute of Science Bangalore India Condensed Matter Physics Division B.A.R.C. Trombay Mumbai Condensed Matter Physics Division Bhabha Atomic Research Centre Trombay Mumbai IMCB The University of Tokyo Tokyo Japan Mitsubishi Chemical Corporation Yokohama Research Center Yokohama Japan Faculty of Pharmaceutical Sciences Chiba University Chiba Japan Department of Biophysics All India Institute of Medical Sciences New Delhi India Department of Biophysics All India Institute of Medical sciences New Delhi India Institüt Physiologische Chemie Hamburg Germany Biophysics Department Bose Institute Calcutta India Slovak Academy of Sciences Institute of Molecular Biology Bratislava Slovakia Indian Institute of Science Bioinformatics Centre Bangalore Departments of Chemistry and Biochemistry Biological Macromolecular Structure Center USA Bulgarian Academy of Sciences Institute of Organic Chemistry Sofia Bulgaria Chinese Academy of Sciences Institute of Biophysics Beijing China Novartis Pharma AG Preclinical Research Basel Switzerland Department of Chemical Sciences Tata Institute of Fundamental Research Mumbai India Solid State Physics Div

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