Phototrophic bacteria and algae are primarily responsible for photosynthesis in aerobic environments, directly harnessing light energy to convert CO₂ into chemical energy. Artificial photosynthesis mimics this process through strategies like the solar-to-fuel cycle, chemically reducing CO₂ into various chemicals. Electrochemical systems, leveraging photovoltaic cells, have been developed to facilitate the transmission of electrons and H₂, generated from water electrolysis, to microbial catalysts for fuel production. Cupriavidus metallidurans CH34, a versatile facultative chemolithotrophic microbe, utilizes Fe-Ni hydrogenase for H₂ dependency. This study delved into the aerobic-autotrophic growth of C. metallidurans under electro-fermentation conditions using a single electrolyzer setup. To demonstrate mimicking photosynthesis based on an electrolyzer, we developed a scalable all-in-one single electrolyzer and applied RppA protein expressed C. metallidurans CH34 strains to confirm malonyl-CoA level measurement. As a result, Single cylinder electrolyzer fermentation appeared to demonstrate the well autotrophic growth, and it produced the malonyl-CoA, which derived chemical as flaviolin was accumulated at 695.9 ± 66.1 mg/L