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European Conference on biomedical Optics, ECBO 2005

作者： Sud, Dhruv Zhong, Wei Mycek, Mary-Ann Dept. of Biomedical Engineering University of Michigan Ann Arbor MI 48109 United States Applied Physics Program University of Michigan Ann Arbor MI 48109 United States Comprehensive Cancer Center University of Michigan Medical School Ann Arbor MI 48109 United States

We developed a controlled-environment FLIM system towards intracellular oxygen sensing, with a resolution of approximately 8 μM, covering the entire physiologic range (0-300 μM). We measured oxygen levels in normal ... 详细信息

ISBN: (纸本)0819458635

关键词： Oxygen

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Computer aided visualizations of 3-dimensional light distributions in tissues for quantitative optical diagnostics

Computer aided visualizations of 3-dimensional light distrib...

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European Conference on biomedical Optics, ECBO 2005

作者： Vishwanath, Karthik Mycek, Mary-Ann Applied Physics Program University of Michigan Ann Arbor MI 48103 United States Dept. of Biomedical Engineering University of Michigan Ann Arbor MI 48109 United States Comprehensive Cancer Center University of Michigan Medical School Ann Arbor MI 48109 United States

Numerical simulations of time-resolved light transport in inhomogeneous tissues reveal quantitative, 3D-distributions of excitation and fluorescent light. Visualizations generated can assist the optimization of endosc... 详细信息

ISBN: (纸本)0819458635

关键词： Visualization

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Fiber optic two-photon fluorescence microendoscopy: Towards brain imaging in freely moving mice

Fiber optic two-photon fluorescence microendoscopy: Towards ...

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2005 Conference on Lasers and Electro-Optics, CLEO

作者： Flusberg, Benjamin A. Jung, Juergen C. Cocker, Eric D. Anderson, Erik P. Schnitzer, Mark J. Department of Applied Physics Stanford University James H. Clark Center for Biomedical Engineering and Sciences Stanford CA 94305-5435 United States Department of Pharmacology Oxford University Mansfield Road Oxford OX1 3QT United Kingdom Department of Biological Sciences Stanford University James H. Clark Center for Biomedical Engineering and Sciences Stanford CA 94305-5435 United States Department of Mechanical Engineering Stanford University James H. Clark Center for Biomedical Engineering and Sciences Stanford CA 94305-5435 United States Department of Electrical Engineering Stanford University James H. Clark Center for Biomedical Engineering and Sciences Stanford CA 94305-5435 United States

We introduce a compact and lightweight (3.7g) two-photon fluorescence microendoscope, which is based on a flexible photonic bandgap fiber and a DC micromotor, and which is designed for brain imaging in freely moving m... 详细信息

ISBN: (纸本)1557527954

关键词： Endoscopy

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Nonlinear Observer Design for an On-line Estimation of the Cerebrospinal Fluid Outflow Resistance

Nonlinear Observer Design for an On-line Estimation of the C...

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IEEE Conference on Decision and Control

作者： A. Shiriaev N. Andersson P.X. Miranda La Hera M. Lilliehook A. Eklund Department of Applied Physics and Electronics Umeå University Sweden Department of Biomedical Engineering and Informatics Umeå University Hospital Umea Sweden

ISBN: (纸本)0780395670

This paper suggests an on-line identification algorithm for estimating a cerebrospinal fluid outflow resistance - one important lumped parameter in the well-known finite-dimensional nonlinear model describing a behaviour of intracranial pressure of humans. The results of on-line tests of the presented algorithm on recorded experimental data are given.

关键词： Immune system Integrated circuit modeling Cranial pressure Testing Equations Parameter estimation physics biomedical engineering biomedical informatics Hospitals

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Collagen content and organization relate to bone nanomechanical properties

Collagen content and organization relate to bone nanomechani...

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2005 Materilas Research Society Spring Meeting

作者： Donnelly, Eve Williams, Rebecca M. Baker, Shefford P. Van der Meulen, Marjolein C. H. Sibley School of Mechanical and Aerospace Engineering Cornell University Ithaca NY United States Department of Applied and Engineering Physics Cornell University Ithaca NY United States Department of Materials Science and Engineering Cornell University Ithaca NY United States Musculoskeletal Integrity Program Hospital for Special Surgery New York NY United States

Cancellous bone plays an important load-bearing role in the skeleton, yet relatively little is known about the microstructure-mechanical property relationships of the tissue at the sub-10 μm level. Cancellous tissue is characterized by a layered microstructure with variable proportions of collagen and mineral. The lamellar material is substantially stiffer than the interlamellar material at the nanomechanical level. However, the microstructural origin of the observed differences in mechanical properties of these structures has not been investigated. In this study, second harmonic generation microscopy was used to examine collagen in human vertebral cancellous bone. At the same location in the tissue, nanoindentation was used to assess the indentation modulus of lamellar and interlamellar bone. The stiff lamellae corresponded to areas of highly ordered, collagen-rich material, while the compliant interlamellar regions corresponded to areas of unoriented or collagen-poor material. The lamellar bone was approximately 30% stiffer and contained approximately 50% more oriented collagen than the interlamellar bone. These observed differences in the mechanical properties and collagen content and organization of lamellar and interlamellar tissue are consistent with previous scanning electron microscopy studies showing greater mineral and collagen content and organization in lamellar bone. Given the well-known coupling between collagen and mineral in bone tissue, the mineral distribution may mirror that of the aligned collagen. However, similar measurements of local variations in mineral content are needed to confirm this hypothesis and may provide additional insights into the tissue nanomechanical behavior. © 2005 Materials Research Society.

关键词： Bone

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Sensing cellular function and molecular activity in vivo using fluorescence lifetime imaging microscopy (FLIM)

Sensing cellular function and molecular activity in vivo usi...

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Novel Optical Instrumentation for biomedical Applications II

作者： Zhong, Wei Sud, Dhruv Wu, Mei Merrick, Karl A. Merajver, Sofia D. Beer, David G. Mycek, Mary-Ann Dept. of Biomedical Engineering University of Michigan Ann Arbor MI 48109 Department of Internal Medicine Medical School University of Michigan Ann Arbor MI 48109 Comprehensive Cancer Center University of Michigan Medical School Ann Arbor MI 48109 Department of Surgery Medical School University of Michigan Ann Arbor MI 48109 Applied Physics Program University of Michigan Ann Arbor MI 48109

In contrast to intensity-based fluorescence microscopy, fluorescence lifetime imaging microscopy (FLIM) bases image contrast on fluorophore excited-state lifetime. This technique is sensitive to the fluorophore's local environment (temperature, ion concentration, dissolved gas concentration, and molecular associations), while being independent of factors impacting fluorescence intensity (fluorophore concentration, photobleaching, scattering, and absorption). We present design features of a novel UV-visible-NIR wide-field time-domain FLIM system with optical sectioning (10 μm), high temporal discrimination (50 ps), and large temporal dynamic range (750 ps - 1 μs), and apply the system to probe cellular metabolic function and detect molecular activity in vivo. © 2005 SPIE and OSA.

关键词： Image processing

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Measurement of intracellular oxygen levels using Fluorescence Lifetime Imaging Microscopy (FLIM)

Measurement of intracellular oxygen levels using Fluorescenc...

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Photon Migration and Diffuse-Light Imaging II

作者： Sud, Dhruv Zhong, Wei Beer, David Mycek, Mary-Ann Dept. of Biomedical Engineering University of Michigan Ann Arbor MI 48109 Thoracic Surgery Section University of Michigan Medical Center Ann Arbor MI 48109 Applied Physics Program University of Michigan Ann Arbor MI 48109 Comprehensive Cancer Center University of Michigan Medical School Ann Arbor MI 48109

Intracellular oxygen levels were measured in vivo under physiological-temperature controlled conditions by monitoring the fluorescence lifetime of the oxygen sensitive dye ruthenium tris(2,2′-dipyridyl) dichloride hexahydrate (RTDP). We employed fluorescence lifetime imaging microscopy (FLIM) and an independent oxygen sensor to calibrate changes in RTDP lifetime with corresponding changes in oxygen level. The FLIM method reproducibly quantified oxygen levels in living human cells cultured from a (Barrett's) adenocarcinoma columnar cell line (SEG-1). Approaches such as those developed here should prove useful for studying oxygen gradients in 3-D biological specimens and for monitoring cellular metabolic status. © 2006 SPIE and OSA.

关键词： Cells

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Fiber optic two-photon fluorescence microendoscopy: towards brain imaging in freely moving mice

Fiber optic two-photon fluorescence microendoscopy: towards ...

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Conference on Lasers and Electro-Optics (CLEO)

作者： B.A. Flusberg J.C. Jung E.D. Cocker E.P. Anderson M.J. Schnitzer Department of Applied Physics James H. Clark Center for Biomedical Engineering and Sciences University of Stanford Stanford CA USA Department of Biological Sciences and Department of Applied Physics James H. Clark for Biomedical Engineering and Sciences University of Stanford Stanford CA USA Department of Pharmacology Oxford University Oxford UK Department of Mechanical Engineering James H. Clark Center for Biomedical Engineering and Sciences University of Stanford Stanford CA USA Department of Electrical Engineering James H. Clark Center for Biomedical Engineering and Sciences University of Stanford Stanford CA USA Department of Biological Sciences and Department of Applied Physics James H. Clark Center for Biomedical Engineering and Sciences University of Stanford Stanford CA USA

We introduce a compact and lightweight (3.7 g) two-photon fluorescence microendoscope, which is based on a flexible photonic bandgap fiber and a DC micromotor, and which is designed for brain imaging in freely moving ... 详细信息

ISBN: (纸本)1557527954

We introduce a compact and lightweight (3.7 g) two-photon fluorescence microendoscope, which is based on a flexible photonic bandgap fiber and a DC micromotor, and which is designed for brain imaging in freely moving mice.

关键词： Optical fibers Fluorescence Brain Optical imaging Mice biomedical engineering Optical microscopy Laser excitation Fiber lasers biomedical optical imaging

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Application of two-photon-absorption photopolymerization to micro-device fabrication

Chung Cheng Ling Hsueh Pao/Journal of Chung Cheng Institute ...

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Chung Cheng Ling Hsueh Pao/Journal of Chung Cheng Institute of Technology 2005年第1期34卷 57-74页

作者： Pan, En-Yea Pu, Nen-Wen Tong, Yuh-Ping Yau, Hon-Fai Department of Applied Physics Chung Cheng Institute of Technology National Defense University Taiwan Medical Engineering Technology Division Biomedical Engineering Center Industrial Technology Research Institute Taiwan Institute of Optical Sciences National Central University Taiwan

In this article we report in detail the fabrication of micro-devices such as micro-rods, micro-wells, micro-ring oscillators, and micro-gratings using two-photon-absorption (TPA) photopolymerization. These micro-devices were written by solidifying liquid ultra-violet sensitive photopolymerizable resins using a focused infrared femtosecond Ti:sapphire laser. Fabrication of high-aspect-ratio, sub-diffraction-limit, sub-micron micro-structures by fast, single-layer writing is achieved using a novel method, which combines the use of a moderate-numerical-aperture objective lens to obtain a much more elongated voxel, and an in-situ ultraviolet pre-exposure step to minimize the required time for subsequent TPA photopolymerization writing.

关键词： High aspect ratio Photopolymerization Two photon absorption

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Gold nanoparticle decoration of DNA on silicon

Gold nanoparticle decoration of DNA on silicon

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2nd Conference on Foundations of Nanoscience: Self-Assembled Architectures and Devices, FNANO 2005

作者： Braun, Gary Inagaki, Katsuhiko Estabrook, R. August Wood, D.K. Levy, Eran Strouse, Geoffrey F. Cleland, A.N. Reich, Norbert O. Department of Chemistry and Biochemistry UC Santa Barbara 93106-9510 United States Biomolecular Science and Engineering Program UC Santa Barbara 93106-9510 United States Department of Physics UC Santa Barbara 93106-9510 United States Department of Applied Physics Hokkaido University Sapporo 060-8628 Japan Department of Chemistry and Biochemistry Florida State University Tallahassee FL 32306-4390 United States

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