检索结果-内蒙古大学图书馆

Evaluation of abilities of Pleurotus pulmonarius crude extract as a natural food additive in roasted chilli paste

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] 2025年 2025 May 20页

作者： Rawita Osit Atchara Nuphet Phoem Bachelor of Science Program in Biotechnology Faculty of Science and Technology Songkhla Rajabhat University Muang Songkhla 90000 Thailand. Bachelor of Science Program in Biotechnology Faculty of Science and Technology Songkhla Rajabhat University Muang Songkhla 90000 Thailand. atchara.ph@skru.ac.th.

The purpose of this study was to determine the effectiveness of the crude extracts in controlling Staphylococcus aureus and Bacillus cereus, as well as the shelf life of the roasted chilli paste. Crude extract was obtained from the volva of Indian oyster mushrooms (Pleurotus pulmonarius) and used to replace preservatives in roasted chilli paste. Thirty isolates of S. aureus and B. cereus were inhibited by crude extract through the disc diffusion method. At a concentration of 2,500 µg/ml, the crude extract inhibited 23 (76.67%) and 20 (66.67%) isolates of S. aureus and B. cereus, respectively, with an inhibition zone size of 7-10 mm. The broth microdilution method was employed in this study to determine the Minimum Inhibitory Concentration (MIC) of the crude extract, and it was observed that the extract at a concentration of 1,000 µg/ml was able to inhibit 18 (60.00%) and 16 (53.00%) isolates of S. aureus and B. cereus, respectively. For the purpose of Minimum Bactericidal Concentration (MBC), the crude extract at a concentration of 1,000 µg/ml was found to kill 16 (53.00%) and 14 (46.00%) isolates of S. aureus and B. cereus, respectively. At a concentration of 4,000 µg/ml, the crude extract had an inhibitory effect from hour 6, lowering S. aureus (Sa16 and Sa21) and B. cereus (Bc5 and Bc12) below the limit of detection (less than 2 log cfu/ml) at 24 h. The ability of the crude extract to inhibit both types of bacteria was observed to be unaffected by heat treatment to a temperature of 65ºC for 30 min (pasteurisation) as well as by no heat treatment at all. The phenolic compounds of the crude extract treated by pasteurisation and without heat treatment were higher than that of the crude extract treated by sterilisation at 121ºC for 15 min. The quality and safety of roasted chilli paste were analysed during storage at 25ºC for 8 months. The quality and safety of roasted chilli paste with crude extract were within the acceptability limit of water activity, total viable

关键词： Food spoilage bacteria Indian oyster mushroom Natural food additive Roasted chilli paste Shelf life extension

来源：评论

学校读者我要写书评

暂无评论

Excessive mitochondrial fission and associated extracellular mitochondria mediate cardiac dysfunction in obesity cardiomyopathy

引用

Life Sciences 2025年 373卷

作者： Li, Sin-Jin Tetri, Laura H. Vijayan, Vijith Elezaby, Aly Chiang, Chun-Hsien Lopez, Ivan Ostberg, Nicolai P. Cornell, Timothy T. Chen, Ching-Yi Haileselassie, Bereketeab Bachelor Program of Biotechnology and Food and Nutrition National Taiwan University Taipei 10617 Taiwan Institute of Biotechnology National Taiwan University Taipei 10617 Taiwan Department of Animal Science and Technology National Taiwan University Taipei 10672 Taiwan Department of Pediatrics Stanford University of School of Medicine Stanford 94305 CA United States Department of Anesthesiology Perioperative and Pain Medicine Stanford University School of Medicine Stanford 94305 CA United States Department of Chemical and Systems Biology Stanford University Stanford 94305 CA United States

Aims: Obesity cardiomyopathy (OCM) is associated with mitochondrial dysfunction caused by altered mitochondrial dynamics. Extracellular mitochondria (exMito) are released following tissue injury under various conditions. While the excessive mitochondrial fission-mediated release of exMito as a mechanism for mitochondrial quality control in several inflammatory disorders, its role in OCM remains unclear. The present work aimed to determine if excessive mitochondrial fission and associated exMito mediate the chronic inflammatory response and cardiac remodeling in OCM. Materials and methods: H9c2 cardiomyoblasts were treated with 200 μM palmitate (PA) to induce lipotoxicity. C57BL/6J mice were fed a high-fat diet (HFD) for 12 weeks to induce OCM. P110, a peptide inhibitor of Drp1/Fis1 interaction, was used to evaluate the impact of excessive mitochondrial fission on cardiac mitochondrial function, quality, and quantity of exMito, systemic inflammatory response, and cardiac contractile function in both models of OCM. Key findings: PA induced excessive mitochondrial fission, increased oxidative stress, decreased ATP level, and damaged exMito release in vitro. Exposure of naïve cardiomyoblasts to exMito isolated from PA treated cells resulted in mitochondrial dysfunction and a pro-inflammatory response. In vivo, HFD induced cardiac mitochondrial and contractile dysfunction, exMito release, and a pro-inflammatory response. Inhibition of Drp1/Fis1 interaction with P110 attenuated the observed effects both in vitro and in vivo. Significance: P110 limited lipid-induced mitochondrial dysfunction and decreased exMito release, subsequently improving the inflammatory state and contractile function in our OCM model. Drp1/Fis1 dependent fission and associated exMito release might serve as a therapeutic target for obesity induced cardiomyopathy. © 2025 Elsevier Inc.

关键词： Extracellular mitochondria Fission Lipotoxicity Mitochondrial dynamics Obesity cardiomyopathy P110

来源：评论

学校读者我要写书评

暂无评论

Legislative preparedness for the control of pandemics – using Taiwan as an example

引用

Medico-Legal Journal 2021年第1期89卷 19-22页

作者： Su, Yi-Chen Bachelor Program of Biotechnology and the Department of Law National Chung Hsing University Taichung City Taiwan

At the early stage of an emerging disease, information is often insufficient for governments to determine what actions are necessary to contain its transmission. Taiwanese society was not prepared when the SARS epidemic hit in 2003. After the SARS epidemic, Taiwan began to overhaul its Communicable Disease Control Act authorising the government to act in a murky situation without the fear of violating due process. In hindsight, the new law has contributed a large part to the effective containment of Covid-19 in Taiwan. However, a new issue emerged concerning the conflict between an individual’s freedom of confidential communication and the government’s use of cell phone positioning to monitor self-quarantine. Although Taiwan’s Council of Grand Justices previously resolved the concern over potential breaching of due-process, the legislature may have to strike a balance between public health emergency and the use of an electronic footprint to trace individual activities. © The Author(s) 2020.

关键词： Covid-19, law, self-quarantine, contact tracing, cell phone position

来源：评论

学校读者我要写书评

暂无评论

Spermidine Carbon Quantum Dotsas an Alternative Antimicrobial Agent for Hatcheries Rearing Pacific White Shrimp (Litopenaeus Vannamei)

SSRN

引用

SSRN 2025年

作者： Lin, Hung-Yun Lin, Han-Tso Chiang, Yun-Ru Lu, Ya-Yun Pan, James Chen, Ming-Hsien Chuang, Jie-Cheng Lin, Hou-Ju Kuo, Wan-Ching Lin, Han-Jia Lin, John Han-You Bachelor Degree Program in Marine Biotechnology National Taiwan Ocean University Keelung City Taiwan Center of Excellence for the Oceans National Taiwan Ocean University Keelung City Taiwan Department of Biotechnology Ming Chuan University Taoyuan City Taiwan School of Veterinary Medicine National Taiwan University Taipei City Taiwan Department of Bilingual Taipei Fuhsing Private School Taipei City Taiwan Sheng Long Bio-tech International Company Long An Province Viet Nam Giant Bio Technology Incorporated New Taipei City Taiwan Department of Bioscience and Biotechnology National Taiwan Ocean University Keelung City Taiwan

Bacterial infections often constrain shrimp production in aquaculture installations. This study assessed the efficacy of a novel antibacterial agent, spermidine carbon quantum dots (spd-CQDs), in promoting the survival of Pacific white shrimp (Litopenaeus vannamei) during the hatching phase and early larval stages;in vitro assessments involved determining the bacteriostatic activity of spd-CQDs against Vibrio strains isolated form shrimps farm in Vietnam and Taiwan. An in vivo challenge was conducted by exposing shrimp larvae to the pathogenic Vibrio parahaemolyticus that causes acute hepatopancreatic necrosis disease (VpAHPND), with or without the inclusion of spd-CQDs in their pellet diet. Results indicate that the application of spd-CQDs significantly reduces Vibrio loads in shrimp hatcheries and enhances the survival rate of larvae in the challenge test. The subsequent analysis assessed the effects of administering spd-CQDs in live feed for broodstock, powder feed for shrimp larvae, and in the rearing water, which can maintain aqua environment quality, demonstrating its ability to maintain the quality of the aquaculture environment. Overall, spd-CQDs significantly reduced Vibrio loads and enhanced the larvae survival rate. This suggests that spd-CQDs could be an alternative to conventional antibiotics, paving the way for sustainable shrimp farming. Future research should address its long-term effects and broader applicability across aquaculture systems. © 2025, The Authors. All rights reserved.

关键词： Bactericides

来源：评论

学校读者我要写书评

暂无评论

Highly selective Cu2+ detection with a naphthalimide-functionalised pillararene fluorescent chemosensor

引用

Organic and Biomolecular Chemistry 2023年第4期22卷 745-752页

作者： Chang, Rong Chen, Chan-Yu Gao, Liya Li, Yana Lee, Zui-Harng Zhao, Hongxia Sue, Andrew C.-H. Chang, Kai-Chi College of Chemistry and Chemical Engineering Xiamen University 422 Siming South Rd Siming District Fujian Province Xiamen361005 China Bachelor Degree Program in Marine Biotechnology National Taiwan Ocean University Keelung202 Taiwan School of Pharmaceutical Science and Technology Tianjin University 92 Weijin Rd Nankai District Tianjin300072 China

Ligand 1, a rim-differentiated pillar[5]arene macrocycle modified with five naphthalimide groups through click chemistry, serves as an effective ratiometric fluorescent chemosensor for Cu2+. In contrast to the monomeric naphthalimide control compound 2, which shows only monomer emission, ligand 1 demonstrates dual emission characteristics encompassing both the monomer and excimer of the naphthalimide moieties. The binding properties of ligand 1 toward 15 different metal ions were systematically investigated in CH2Cl2/CH3CN (v/v, 1 : 1) by UV-vis and fluorescence spectroscopy. Remarkably, ligand 1 exhibits exceptional selectivity for Cu2+ ions. Upon complexation with Cu2+, the excimer emission of ligand 1 diminishes, concomitant with an enhancement of its monomer emission. The binding ratio for 1·Cu2+ was determined to be 1 : 1, with an association constant of (3.39 ± 0.40) × 105 M−1 calculated using a nonlinear least-squares curve-fitting method. Furthermore, the limit of detection (LOD) was found to be 185 ± 7 nM. Our results from 1H NMR titration, high-resolution mass spectrometry analysis and density functional theory calculations of 1·Cu2+ suggest synergistic coordination between Cu2+ and the triazole groups on ligand 1. © 2024 The Royal Society of Chemistry.

关键词： Ligands

来源：评论

学校读者我要写书评

暂无评论

Structural Variants and Ultralow Detection Ability for Tryptamine in Two Polymorphs of a Zincophosphite Framework

引用

Inorganic Chemistry 2023年第44期62卷 18150-18156页

作者： Yin, Mu-Chien Wei, Pi-Chen Li, Ying Hsu, Todd Jian, Jia-Yi Chang, Kai-Chi Lu, Ching-Ping Tu, Hsiung-Lin Wang, Chih-Min Department of Bioscience and Biotechnology National Taiwan Ocean University 202 Keelung20224 Taiwan Bachelor Degree Program in Marine Biotechnology National Taiwan Ocean University 202 Keelung20224 Taiwan Department of Environmental Biology and Fisheries Science National Taiwan Ocean University 202 Keelung20224 Taiwan Institute of Chemistry Academia Sinica Taipei11529 Taiwan Genome and Systems Biology Degree Program Academia Sinica and National Taiwan University Taipei11529 Taiwan General Education Center National Taiwan Ocean University 202 Keelung20224 Taiwan

Two organic-inorganic hybrid zinc phosphites incorporating 1,2,4,5-tetrakis(imidazol-1-ylmethyl)benzene (TIMB) molecules were synthesized under hydro(solvo)thermal methods and structurally characterized by single-crystal X-ray diffraction (SCXD). Interestingly, the solvent ratio of water to dimethylformamide induced the formation of a new compound of Zn2(TIMB)0.5(HPO3)2·3H2O (1) and our previously reported structure of Zn2(TIMB)0.5(HPO3)2·H2O (2). Additionally, their dehydrated crystals (1a and 2a) were prepared through heat treatment at 150 °C. SCXD and powder X-ray diffraction showed that all four compounds share the same framework formula of Zn2(TIMB)0.5(HPO3)2 but exhibit a huge difference in their inorganic components and final structures. In 1 and 1a, the inorganic units formed two-dimensional zincophosphite layers, while in 2 and 2a, they formed one-dimensional chains. The inorganic parts of 1 (1a) and 2 (2a) were bridged with TIMB linkers, resulting in 3D structures with rectangular and tubular windows, respectively. Furthermore, 1 was coated on the screen-printed carbon electron as a hybrid material, displaying excellent performance while having a linear relationship with an R2 value of 0.99 within the concentration range of 10-10 to 10-6 mol/L for detecting tryptamine (Try) molecules. Moreover, the results showed that 1 exhibits an ultralow limit of detection of 5.43 × 10-11 mol/L and high specificity toward Try over histamine, ascorbic acid, uric acid, and glucose. The synthesis, structural diversity, stability, and sensing ability are also discussed. © 2023 American Chemical Society.

关键词： Single crystals

来源：评论

学校读者我要写书评

暂无评论

Mesoporous silica nanoparticles with dual-targeting agricultural sources for enhanced cancer treatment via tritherapy

引用

RSC Advances 2023年第28期13卷 19079-19090页

作者： Huang, Yu-Ya Lee, Zui-Harng Chang, Kai-Chi Wu, Zhi-Yuan Lee, Cheng-Chang Tsou, Min-Hsuan Lin, Hsiu-Mei National Taiwan Ocean University Department of Bioscience and Biotechnology No. 2 Beining Rd. Zhongzheng Dist. Keelung City202 Taiwan National Taiwan Ocean University Bachelor Degree Program in Marine Biotechenology Taiwan National Taiwan Ocean University Center of Excellence for the Oceans Taiwan National Taiwan Ocean University Center of Excellence for Ocean Engineering Taiwan

In this study, we introduced dual-targeting folic acid (FA) and hyaluronic acid (HA) modified on the surface of rice husk mesoporous silica nanoparticles (rMSNs). The rMSNs were employed as a drug delivery system loaded with camptothecin (CPT) as a model drug, Eu3+ ions as a photosensitizer for photodynamic therapy (PDT), bismuth (Bi) for photothermal therapy (PTT), and Gd3+ ions for magnetic resonance imaging (MRI) to develop novel nanoparticles, rMSN-EuGd-Bi@CPT-HA-FA, with dual-targeted function and triple therapy for cancer treatment. The results of the cell cytotoxicity experiment showed that the A549 cancer cells had a survival rate of approximately 35% when treated with 200 μg mL−1 of rMSN-EuGd-Bi@CPT-HA-FA under 808 nm irradiation for 15 min. The dual-targeted function and synergistic treatment of CPT, PTT, and PDT were also responsible for the 20% survival rate of the A549 cancer cells treated with 200 μg mL−1 of rMSN-EuGd-Bi@CPT-HA-FA under 808 nm irradiation for 30 min. The results showed that rMSN-EuGd-Bi@CPT-HA-FA can effectively combine chemotherapy (through CPT), PDT, and PTT for cancer treatment. © 2023 The Royal Society of Chemistry.

关键词： Hyaluronic acid

来源：评论

学校读者我要写书评

暂无评论

Potato protein hydrolysate inhibits RANKL-induced osteoclast development by inhibiting osteoclastogenic genes via the NF-κB/MAPKs signaling pathways

引用

Environmental Toxicology 2024年第7期39卷 3991-4003页

作者： Chen, Yi-Ju He, Yen-Hua Lo, Yun-Hsin Yang, Hong-Siang Abomughaid, Mosleh Mohammad Kumar, K. J. Senthil Lin, Wan-Teng Department of Surgery Taichung Veterans General Hospital Taichung Taiwan Department of Animal Science and Biotechnology College of Agriculture and Health Tunghai University Taichung Taiwan Department of Food Science College of Agriculture and Health Tunghai University Taichung Taiwan Department of Hospitality Management College of Agriculture and Health Tunghai University Taichung Taiwan Department of Medical Laboratory Sciences College of Applied Medical Sciences University of Bisha Bisha Saudi Arabia Bachelor Program of Biotechnology and Center for General Education National Chung Hsing University Taichung Taiwan Research and Development Division Utopia Holiday Hotel Corporation Taichung Taiwan

In recent times, there has been growing attention towards exploring the nutritional and functional aspects of potato protein, along with its diverse applications. In the present study, we examined the anti-osteoclast properties of potato protein hydrolysate (PP902) in vitro. Murine macrophages (RAW264.7) were differentiated into osteoclasts by receptor activator of nuclear factor-κB ligand (RANKL), and PP902 was examined for its inhibitory effect. Initially, treatment with PP902 was found to significantly prevent RANKL-induced morphological changes in macrophage cells, as determined by tartrate-resistant acid phosphatase (TRAP) staining analysis. This notion was further supported by F-actin analysis using a confocal microscope. Furthermore, PP902 treatment effectively and dose-dependently down-regulated the expression of RANKL-induced osteoclastogenic marker genes, including TRAP, CTR, RANK, NFATc1, OC-STAMP, and c-Fos. These inhibitory effects were associated with suppressing NF-κB transcriptional activation and subsequent reduced nuclear translocation. The decrease in NF-κB activity resulted from reduced activation of its upstream kinases, including I-κBα and IKKα. Moreover, PP902 significantly inhibited RANKL-induced p38MAPK and ERK1/2 activities. Nevertheless, PP902 treatment prevents RANKL-induced intracellular reactive oxygen species generation via increased HO-1 activity. The combined antioxidant and anti-inflammatory effects of PP902 resulted in significant suppression of osteoclastogenesis, suggesting its potential as an adjuvant therapy for osteoclast-related diseases. © 2024 Wiley Periodicals LLC.

关键词： Genes

来源：评论

学校读者我要写书评

暂无评论

Targeting protein-protein interaction interfaces with antiviral N protein inhibitor in SARS-CoV-2

引用

Biophysical Journal 2024年第4期123卷 478-488页

作者： Hong, Jhen-Yi Lin, Shih-Chao Kehn-Hall, Kylene Zhang, Kai-Min Luo, Shun-Yuan Wu, Hung-Yi Chang, Sui-Yuan Hou, Ming-Hon Institute of Genomics and Bioinformatics and Department of Life Sciences National Chung Hsing University Taichung Taiwan Bachelor Degree Program in Marine Biotechnology College of Life Sciences National Taiwan Ocean University Keelung Taiwan Department of Biomedical Sciences and Pathobiology Virginia-Maryland College of Veterinary Medicine Virginia Polytechnic Institute and State University Blacksburg VA United States Center for Emerging Zoonotic and Arthropod-borne Pathogens Virginia Polytechnic Institute and State University Blacksburg VA United States Department of Chemistry National Chung Hsing University Taichung Taiwan Graduate Institute of Veterinary Pathobiology College of Veterinary Medicine National Chung Hsing University. Taichung Taiwan Department of Clinical Laboratory Sciences and Medical Biotechnology College of Medicine National Taiwan University Taipei Taiwan PhD Program in Medical Biotechnology National Chung Hsing University Taichung Taiwan Biotechnology Center National Chung Hsing University Taichung Taiwan

Coronaviruses not only pose significant global public health threats but also cause extensive damage to livestock-based industries. Previous studies have shown that 5-benzyloxygramine (P3) targets the Middle East respiratory syndrome coronavirus (MERS-CoV) nucleocapsid (N) protein N-terminal domain (N-NTD), inducing non-native protein-protein interactions (PPIs) that impair N protein function. Moreover, P3 exhibits broad-spectrum antiviral activity against CoVs. The sequence similarity of N proteins is relatively low among CoVs, further exhibiting notable variations in the hydrophobic residue responsible for non-native PPIs in the N-NTD. Therefore, to ascertain the mechanism by which P3 demonstrates broad-spectrum anti-CoV activity, we determined the crystal structure of the SARS-CoV-2 N-NTD:P3 complex. We found that P3 was positioned in the dimeric N-NTD via hydrophobic contacts. Compared with the interfaces in MERS-CoV N-NTD, P3 had a reversed orientation in SARS-CoV-2 N-NTD. The Phe residue in the MERS-CoV N-NTD:P3 complex stabilized both P3 moieties. However, in the SARS-CoV-2 N-NTD:P3 complex, the Ile residue formed only one interaction with the P3 benzene ring. Moreover, the pocket in the SARS-CoV-2 N-NTD:P3 complex was more hydrophobic, favoring the insertion of the P3 benzene ring into the complex. Nevertheless, hydrophobic interactions remained the primary stabilizing force in both complexes. These findings suggested that despite the differences in the sequence, P3 can accommodate a hydrophobic pocket in N-NTD to mediate a non-native PPI, enabling its effectiveness against various CoVs. © 2024 Biophysical Society

关键词：

来源：评论

学校读者我要写书评

暂无评论

Antcin A, a phytosterol regulates SARS-CoV-2 spike protein-mediated metabolic alteration in THP-1 cells explored by the ¹H-NMR-based metabolomics approach

引用

Phytotherapy Research 2023年第3期37卷 885-902页

作者： Dakpa, Gyaltsen Senthil Kumar, Kanthasamy Jayabal Tsao, Nai-Wen Wang, Sheng-Yang Molecular and Biological Agricultural Sciences Program Taiwan International Graduate Program Academia Sinica Taipei Taiwan Graduate Institute of Biotechnology National Chung-Hsing University Taichung Taiwan Bachelor Program of Biotechnology National Chung Hsing University Taichung Taiwan Department of Forestry National Chung-Hsing University Taichung Taiwan Agricultural Biotechnology Research Center Academia Sinica Taipei Taiwan

The mechanism of SARS-CoV-2 spike protein-mediated perturbations of metabolic pathways and modulation of antcin A, a steroid-like compound isolated from Taiwanofungus camphoratus, are not studied. Here, we investigated the metabolic alteration by SARS-CoV-2 spike protein and the regulatory effect of antcin A on SARS-CoV-2 spike protein-induced metabolic changes in the Phorbol 12-myristate 13-acetate (PMA)-induced human monocytes (THP-1) using proton nuclear magnetic resonance (¹H-NMR) and MetaboAnalyst 5.0 software. The cytotoxic potential of SARS-CoV-2 spike protein, antcin A, and dexamethasone was assessed by MTT assay. The metabolomic perturbations and their relation to human coronaviruses' receptors were evaluated by qPCR. This study indicated that the altered metabolites mediated by SARS-CoV-2 protein, such as methionine, phosphoenolpyruvic acid, canadine, glutamine, ethanolamine, and phenylalanine, were significantly reversed by antcin A. In addition, antcin A significantly inhibited SARS-CoV-2 spike protein-mediated up-regulation of TLR-4 and ACE2 receptors, while GRP78 inhibition was not statistically significant. This is the first study to use ¹H-NMR to investigate SARS-CoV-2 spike protein-induced metabolomic changes in PMA-induced THP-1 cells. Antcin A significantly reversed metabolomic alters while dexamethasone failed to fix them. Therefore, we believe that antcin A could be a potential candidate for therapeutic agents for viral infections related to a metabolic abnormality. © 2022 John Wiley & Sons Ltd.

关键词： 1H-NMR antcin A metabolomics SARS-CoV-2 spike protein Taiwanofungus camphoratus

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：