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Evaluation of abilities of Pleurotus pulmonarius crude extract as a natural food additive in roasted chilli paste

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] 2025年 2025 May 20页

作者： Rawita Osit Atchara Nuphet Phoem Bachelor of Science Program in Biotechnology Faculty of Science and Technology Songkhla Rajabhat University Muang Songkhla 90000 Thailand. Bachelor of Science Program in Biotechnology Faculty of Science and Technology Songkhla Rajabhat University Muang Songkhla 90000 Thailand. atchara.ph@skru.ac.th.

The purpose of this study was to determine the effectiveness of the crude extracts in controlling Staphylococcus aureus and Bacillus cereus, as well as the shelf life of the roasted chilli paste. Crude extract was obtained from the volva of Indian oyster mushrooms (Pleurotus pulmonarius) and used to replace preservatives in roasted chilli paste. Thirty isolates of S. aureus and B. cereus were inhibited by crude extract through the disc diffusion method. At a concentration of 2,500 µg/ml, the crude extract inhibited 23 (76.67%) and 20 (66.67%) isolates of S. aureus and B. cereus, respectively, with an inhibition zone size of 7-10 mm. The broth microdilution method was employed in this study to determine the Minimum Inhibitory Concentration (MIC) of the crude extract, and it was observed that the extract at a concentration of 1,000 µg/ml was able to inhibit 18 (60.00%) and 16 (53.00%) isolates of S. aureus and B. cereus, respectively. For the purpose of Minimum Bactericidal Concentration (MBC), the crude extract at a concentration of 1,000 µg/ml was found to kill 16 (53.00%) and 14 (46.00%) isolates of S. aureus and B. cereus, respectively. At a concentration of 4,000 µg/ml, the crude extract had an inhibitory effect from hour 6, lowering S. aureus (Sa16 and Sa21) and B. cereus (Bc5 and Bc12) below the limit of detection (less than 2 log cfu/ml) at 24 h. The ability of the crude extract to inhibit both types of bacteria was observed to be unaffected by heat treatment to a temperature of 65ºC for 30 min (pasteurisation) as well as by no heat treatment at all. The phenolic compounds of the crude extract treated by pasteurisation and without heat treatment were higher than that of the crude extract treated by sterilisation at 121ºC for 15 min. The quality and safety of roasted chilli paste were analysed during storage at 25ºC for 8 months. The quality and safety of roasted chilli paste with crude extract were within the acceptability limit of water activity, total viable

关键词： Food spoilage bacteria Indian oyster mushroom Natural food additive Roasted chilli paste Shelf life extension

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Excessive mitochondrial fission and associated extracellular mitochondria mediate cardiac dysfunction in obesity cardiomyopathy

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Life Sciences 2025年 373卷

作者： Li, Sin-Jin Tetri, Laura H. Vijayan, Vijith Elezaby, Aly Chiang, Chun-Hsien Lopez, Ivan Ostberg, Nicolai P. Cornell, Timothy T. Chen, Ching-Yi Haileselassie, Bereketeab Bachelor Program of Biotechnology and Food and Nutrition National Taiwan University Taipei 10617 Taiwan Institute of Biotechnology National Taiwan University Taipei 10617 Taiwan Department of Animal Science and Technology National Taiwan University Taipei 10672 Taiwan Department of Pediatrics Stanford University of School of Medicine Stanford 94305 CA United States Department of Anesthesiology Perioperative and Pain Medicine Stanford University School of Medicine Stanford 94305 CA United States Department of Chemical and Systems Biology Stanford University Stanford 94305 CA United States

Aims: Obesity cardiomyopathy (OCM) is associated with mitochondrial dysfunction caused by altered mitochondrial dynamics. Extracellular mitochondria (exMito) are released following tissue injury under various conditions. While the excessive mitochondrial fission-mediated release of exMito as a mechanism for mitochondrial quality control in several inflammatory disorders, its role in OCM remains unclear. The present work aimed to determine if excessive mitochondrial fission and associated exMito mediate the chronic inflammatory response and cardiac remodeling in OCM. Materials and methods: H9c2 cardiomyoblasts were treated with 200 μM palmitate (PA) to induce lipotoxicity. C57BL/6J mice were fed a high-fat diet (HFD) for 12 weeks to induce OCM. P110, a peptide inhibitor of Drp1/Fis1 interaction, was used to evaluate the impact of excessive mitochondrial fission on cardiac mitochondrial function, quality, and quantity of exMito, systemic inflammatory response, and cardiac contractile function in both models of OCM. Key findings: PA induced excessive mitochondrial fission, increased oxidative stress, decreased ATP level, and damaged exMito release in vitro. Exposure of naïve cardiomyoblasts to exMito isolated from PA treated cells resulted in mitochondrial dysfunction and a pro-inflammatory response. In vivo, HFD induced cardiac mitochondrial and contractile dysfunction, exMito release, and a pro-inflammatory response. Inhibition of Drp1/Fis1 interaction with P110 attenuated the observed effects both in vitro and in vivo. Significance: P110 limited lipid-induced mitochondrial dysfunction and decreased exMito release, subsequently improving the inflammatory state and contractile function in our OCM model. Drp1/Fis1 dependent fission and associated exMito release might serve as a therapeutic target for obesity induced cardiomyopathy. © 2025 Elsevier Inc.

关键词： Extracellular mitochondria Fission Lipotoxicity Mitochondrial dynamics Obesity cardiomyopathy P110

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Legislative preparedness for the control of pandemics – using Taiwan as an example

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Medico-Legal Journal 2021年第1期89卷 19-22页

作者： Su, Yi-Chen Bachelor Program of Biotechnology and the Department of Law National Chung Hsing University Taichung City Taiwan

At the early stage of an emerging disease, information is often insufficient for governments to determine what actions are necessary to contain its transmission. Taiwanese society was not prepared when the SARS epidemic hit in 2003. After the SARS epidemic, Taiwan began to overhaul its Communicable Disease Control Act authorising the government to act in a murky situation without the fear of violating due process. In hindsight, the new law has contributed a large part to the effective containment of Covid-19 in Taiwan. However, a new issue emerged concerning the conflict between an individual’s freedom of confidential communication and the government’s use of cell phone positioning to monitor self-quarantine. Although Taiwan’s Council of Grand Justices previously resolved the concern over potential breaching of due-process, the legislature may have to strike a balance between public health emergency and the use of an electronic footprint to trace individual activities. © The Author(s) 2020.

关键词： Covid-19, law, self-quarantine, contact tracing, cell phone position

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Antcin A, a phytosterol regulates SARS-CoV-2 spike protein-mediated metabolic alteration in THP-1 cells explored by the ¹H-NMR-based metabolomics approach

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Phytotherapy Research 2023年第3期37卷 885-902页

作者： Dakpa, Gyaltsen Senthil Kumar, Kanthasamy Jayabal Tsao, Nai-Wen Wang, Sheng-Yang Molecular and Biological Agricultural Sciences Program Taiwan International Graduate Program Academia Sinica Taipei Taiwan Graduate Institute of Biotechnology National Chung-Hsing University Taichung Taiwan Bachelor Program of Biotechnology National Chung Hsing University Taichung Taiwan Department of Forestry National Chung-Hsing University Taichung Taiwan Agricultural Biotechnology Research Center Academia Sinica Taipei Taiwan

The mechanism of SARS-CoV-2 spike protein-mediated perturbations of metabolic pathways and modulation of antcin A, a steroid-like compound isolated from Taiwanofungus camphoratus, are not studied. Here, we investigated the metabolic alteration by SARS-CoV-2 spike protein and the regulatory effect of antcin A on SARS-CoV-2 spike protein-induced metabolic changes in the Phorbol 12-myristate 13-acetate (PMA)-induced human monocytes (THP-1) using proton nuclear magnetic resonance (¹H-NMR) and MetaboAnalyst 5.0 software. The cytotoxic potential of SARS-CoV-2 spike protein, antcin A, and dexamethasone was assessed by MTT assay. The metabolomic perturbations and their relation to human coronaviruses' receptors were evaluated by qPCR. This study indicated that the altered metabolites mediated by SARS-CoV-2 protein, such as methionine, phosphoenolpyruvic acid, canadine, glutamine, ethanolamine, and phenylalanine, were significantly reversed by antcin A. In addition, antcin A significantly inhibited SARS-CoV-2 spike protein-mediated up-regulation of TLR-4 and ACE2 receptors, while GRP78 inhibition was not statistically significant. This is the first study to use ¹H-NMR to investigate SARS-CoV-2 spike protein-induced metabolomic changes in PMA-induced THP-1 cells. Antcin A significantly reversed metabolomic alters while dexamethasone failed to fix them. Therefore, we believe that antcin A could be a potential candidate for therapeutic agents for viral infections related to a metabolic abnormality. © 2022 John Wiley & Sons Ltd.

关键词： 1H-NMR antcin A metabolomics SARS-CoV-2 spike protein Taiwanofungus camphoratus

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Spermidine Carbon Quantum Dotsas an Alternative Antimicrobial Agent for Hatcheries Rearing Pacific White Shrimp (Litopenaeus Vannamei)

SSRN

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SSRN 2025年

作者： Lin, Hung-Yun Lin, Han-Tso Chiang, Yun-Ru Lu, Ya-Yun Pan, James Chen, Ming-Hsien Chuang, Jie-Cheng Lin, Hou-Ju Kuo, Wan-Ching Lin, Han-Jia Lin, John Han-You Bachelor Degree Program in Marine Biotechnology National Taiwan Ocean University Keelung City Taiwan Center of Excellence for the Oceans National Taiwan Ocean University Keelung City Taiwan Department of Biotechnology Ming Chuan University Taoyuan City Taiwan School of Veterinary Medicine National Taiwan University Taipei City Taiwan Department of Bilingual Taipei Fuhsing Private School Taipei City Taiwan Sheng Long Bio-tech International Company Long An Province Viet Nam Giant Bio Technology Incorporated New Taipei City Taiwan Department of Bioscience and Biotechnology National Taiwan Ocean University Keelung City Taiwan

Bacterial infections often constrain shrimp production in aquaculture installations. This study assessed the efficacy of a novel antibacterial agent, spermidine carbon quantum dots (spd-CQDs), in promoting the survival of Pacific white shrimp (Litopenaeus vannamei) during the hatching phase and early larval stages;in vitro assessments involved determining the bacteriostatic activity of spd-CQDs against Vibrio strains isolated form shrimps farm in Vietnam and Taiwan. An in vivo challenge was conducted by exposing shrimp larvae to the pathogenic Vibrio parahaemolyticus that causes acute hepatopancreatic necrosis disease (VpAHPND), with or without the inclusion of spd-CQDs in their pellet diet. Results indicate that the application of spd-CQDs significantly reduces Vibrio loads in shrimp hatcheries and enhances the survival rate of larvae in the challenge test. The subsequent analysis assessed the effects of administering spd-CQDs in live feed for broodstock, powder feed for shrimp larvae, and in the rearing water, which can maintain aqua environment quality, demonstrating its ability to maintain the quality of the aquaculture environment. Overall, spd-CQDs significantly reduced Vibrio loads and enhanced the larvae survival rate. This suggests that spd-CQDs could be an alternative to conventional antibiotics, paving the way for sustainable shrimp farming. Future research should address its long-term effects and broader applicability across aquaculture systems. © 2025, The Authors. All rights reserved.

关键词： Bactericides

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Insights in nodule-inhabiting plant growth promoting bacteria and their ability to stimulate Vicia faba growth

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Egyptian Journal of Basic and Applied Sciences 2022年第1期9卷 51-64页

作者： M. Mowafy, Amr S. Agha, Mona A. Haroun, Samia A. Abbas, Mohamed Elbalkini, Mohamed Botany Department Faculty of Science Mansoura University Mansoura Egypt Department of Biological Sciences Faculty of Science New Mansoura University New Mansoura City Egypt Bachelor Student Biotechnology and Its Application Program Faculty of Science Mansoura University Mansoura Egypt

The knowledge of the root nodule microbiome is still beyond the minimum. This study has been designed to isolate and characterize plant growth promoting bacteria and testing their ability to promote plant growth. In addition to Rhizobium MAP7, four other strains have been isolated from Phaseolus vulgaris nodules, and they have been molecularly identified as Bacillus MAP3, Brevibacillus MAP4, Pseudomonas MAP5, and Pseudomonas MAP8. The obtained strains showed the ability to produce IAA, siderophores, and HCN with different capabilities. A pot experiment was conducted to investigate the effect of the obtained strains on the physical growth parameters of Vicia faba. The inoculation with Rhizobium MAP7 along with Brevibacillus MAP4 and Pseudomonas MAP5 led to four times increase in the number of nodules (57/plant) compared with that of the control in addition to a significant increase in the physical growth parameters . Additionally, different nitrogen fractions increased significantly in response to this inoculum (226 and 206 mg/g dry weight total nitrogen for shoot and root respectively compared with 175 and 92.5 for control plants). The obtained strains in this study showed a good potentiality as plant growth promotors that might be tried with further crops on wider scale. © 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group.

关键词： Brevibacillus broad bean co-culture pseudomonas rhizobium siderophores

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Targeting an allosteric site in dynamin-related protein 1 to inhibit Fis1-mediated mitochondrial dysfunction

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Nature Communications 2023年第1期14卷 4356页

作者： Rios, Luis Pokhrel, Suman Li, Sin-Jin Heo, Gwangbeom Haileselassie, Bereketeab Mochly-Rosen, Daria Department of Chemical and Systems Biology Stanford University School of Medicine Stanford CA United States Department of Pediatrics Stanford University School of Medicine Stanford CA United States Bachelor Program of Biotechnology and Food Nutrition National Taiwan University Taipei City Taiwan

The large cytosolic GTPase, dynamin-related protein 1 (Drp1), mediates both physiological and pathological mitochondrial fission. Cell stress triggers Drp1 binding to mitochondrial Fis1 and subsequently, mitochondrial fragmentation, ROS production, metabolic collapse, and cell death. Because Drp1 also mediates physiological fission by binding to mitochondrial Mff, therapeutics that inhibit pathological fission should spare physiological mitochondrial fission. P110, a peptide inhibitor of Drp1-Fis1 interaction, reduces pathology in numerous models of neurodegeneration, ischemia, and sepsis without blocking the physiological functions of Drp1. Since peptides have pharmacokinetic limitations, we set out to identify small molecules that mimic P110’s benefit. We map the P110-binding site to a switch I-adjacent grove (SWAG) on Drp1. Screening for SWAG-binding small molecules identifies SC9, which mimics P110’s benefits in cells and a mouse model of endotoxemia. We suggest that the SWAG-binding small molecules discovered in this study may reduce the burden of Drp1-mediated pathologies and potentially pathologies associated with other members of the GTPase family. © 2023, The Author(s).

关键词： Mitochondria Molecular modelling Small molecules Target validation

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Highly selective Cu2+ detection with a naphthalimide-functionalised pillararene fluorescent chemosensor

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Organic and Biomolecular Chemistry 2023年第4期22卷 745-752页

作者： Chang, Rong Chen, Chan-Yu Gao, Liya Li, Yana Lee, Zui-Harng Zhao, Hongxia Sue, Andrew C.-H. Chang, Kai-Chi College of Chemistry and Chemical Engineering Xiamen University 422 Siming South Rd Siming District Fujian Province Xiamen361005 China Bachelor Degree Program in Marine Biotechnology National Taiwan Ocean University Keelung202 Taiwan School of Pharmaceutical Science and Technology Tianjin University 92 Weijin Rd Nankai District Tianjin300072 China

Ligand 1, a rim-differentiated pillar[5]arene macrocycle modified with five naphthalimide groups through click chemistry, serves as an effective ratiometric fluorescent chemosensor for Cu2+. In contrast to the monomeric naphthalimide control compound 2, which shows only monomer emission, ligand 1 demonstrates dual emission characteristics encompassing both the monomer and excimer of the naphthalimide moieties. The binding properties of ligand 1 toward 15 different metal ions were systematically investigated in CH2Cl2/CH3CN (v/v, 1 : 1) by UV-vis and fluorescence spectroscopy. Remarkably, ligand 1 exhibits exceptional selectivity for Cu2+ ions. Upon complexation with Cu2+, the excimer emission of ligand 1 diminishes, concomitant with an enhancement of its monomer emission. The binding ratio for 1·Cu2+ was determined to be 1 : 1, with an association constant of (3.39 ± 0.40) × 105 M−1 calculated using a nonlinear least-squares curve-fitting method. Furthermore, the limit of detection (LOD) was found to be 185 ± 7 nM. Our results from 1H NMR titration, high-resolution mass spectrometry analysis and density functional theory calculations of 1·Cu2+ suggest synergistic coordination between Cu2+ and the triazole groups on ligand 1. © 2024 The Royal Society of Chemistry.

关键词： Ligands

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Structural Variants and Ultralow Detection Ability for Tryptamine in Two Polymorphs of a Zincophosphite Framework

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Inorganic Chemistry 2023年第44期62卷 18150-18156页

作者： Yin, Mu-Chien Wei, Pi-Chen Li, Ying Hsu, Todd Jian, Jia-Yi Chang, Kai-Chi Lu, Ching-Ping Tu, Hsiung-Lin Wang, Chih-Min Department of Bioscience and Biotechnology National Taiwan Ocean University 202 Keelung20224 Taiwan Bachelor Degree Program in Marine Biotechnology National Taiwan Ocean University 202 Keelung20224 Taiwan Department of Environmental Biology and Fisheries Science National Taiwan Ocean University 202 Keelung20224 Taiwan Institute of Chemistry Academia Sinica Taipei11529 Taiwan Genome and Systems Biology Degree Program Academia Sinica and National Taiwan University Taipei11529 Taiwan General Education Center National Taiwan Ocean University 202 Keelung20224 Taiwan

Two organic-inorganic hybrid zinc phosphites incorporating 1,2,4,5-tetrakis(imidazol-1-ylmethyl)benzene (TIMB) molecules were synthesized under hydro(solvo)thermal methods and structurally characterized by single-crystal X-ray diffraction (SCXD). Interestingly, the solvent ratio of water to dimethylformamide induced the formation of a new compound of Zn2(TIMB)0.5(HPO3)2·3H2O (1) and our previously reported structure of Zn2(TIMB)0.5(HPO3)2·H2O (2). Additionally, their dehydrated crystals (1a and 2a) were prepared through heat treatment at 150 °C. SCXD and powder X-ray diffraction showed that all four compounds share the same framework formula of Zn2(TIMB)0.5(HPO3)2 but exhibit a huge difference in their inorganic components and final structures. In 1 and 1a, the inorganic units formed two-dimensional zincophosphite layers, while in 2 and 2a, they formed one-dimensional chains. The inorganic parts of 1 (1a) and 2 (2a) were bridged with TIMB linkers, resulting in 3D structures with rectangular and tubular windows, respectively. Furthermore, 1 was coated on the screen-printed carbon electron as a hybrid material, displaying excellent performance while having a linear relationship with an R2 value of 0.99 within the concentration range of 10-10 to 10-6 mol/L for detecting tryptamine (Try) molecules. Moreover, the results showed that 1 exhibits an ultralow limit of detection of 5.43 × 10-11 mol/L and high specificity toward Try over histamine, ascorbic acid, uric acid, and glucose. The synthesis, structural diversity, stability, and sensing ability are also discussed. © 2023 American Chemical Society.

关键词： Single crystals

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Mesoporous silica nanoparticles with dual-targeting agricultural sources for enhanced cancer treatment via tritherapy

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RSC Advances 2023年第28期13卷 19079-19090页

作者： Huang, Yu-Ya Lee, Zui-Harng Chang, Kai-Chi Wu, Zhi-Yuan Lee, Cheng-Chang Tsou, Min-Hsuan Lin, Hsiu-Mei National Taiwan Ocean University Department of Bioscience and Biotechnology No. 2 Beining Rd. Zhongzheng Dist. Keelung City202 Taiwan National Taiwan Ocean University Bachelor Degree Program in Marine Biotechenology Taiwan National Taiwan Ocean University Center of Excellence for the Oceans Taiwan National Taiwan Ocean University Center of Excellence for Ocean Engineering Taiwan

In this study, we introduced dual-targeting folic acid (FA) and hyaluronic acid (HA) modified on the surface of rice husk mesoporous silica nanoparticles (rMSNs). The rMSNs were employed as a drug delivery system loaded with camptothecin (CPT) as a model drug, Eu3+ ions as a photosensitizer for photodynamic therapy (PDT), bismuth (Bi) for photothermal therapy (PTT), and Gd3+ ions for magnetic resonance imaging (MRI) to develop novel nanoparticles, rMSN-EuGd-Bi@CPT-HA-FA, with dual-targeted function and triple therapy for cancer treatment. The results of the cell cytotoxicity experiment showed that the A549 cancer cells had a survival rate of approximately 35% when treated with 200 μg mL−1 of rMSN-EuGd-Bi@CPT-HA-FA under 808 nm irradiation for 15 min. The dual-targeted function and synergistic treatment of CPT, PTT, and PDT were also responsible for the 20% survival rate of the A549 cancer cells treated with 200 μg mL−1 of rMSN-EuGd-Bi@CPT-HA-FA under 808 nm irradiation for 30 min. The results showed that rMSN-EuGd-Bi@CPT-HA-FA can effectively combine chemotherapy (through CPT), PDT, and PTT for cancer treatment. © 2023 The Royal Society of Chemistry.

关键词： Hyaluronic acid

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