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Optimization, characterization, and molecular modelling of an acid and organic solvent-tolerant lipase isolated from Monascus pilosus as a potential detergent additive

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International Journal of Biological Macromolecules 2025年 317卷

作者： Mohamed M.M. Ahmed Sherif F. Hammad Ahmed L. Abdel-Mawgood Biotechnology Program Institute of Basic and Applied Science Egypt-Japan University of Science and Technology Alexandria 21934 Egypt Botany Department Faculty of Science Aswan University Aswan 81528 Egypt Department of Pharmaceutical Chemistry Faculty of Pharmacy Helwan University Helwan Cairo 11795 Egypt PharmD Program Egypt-Japan University of Science and Technology (E-JUST) New Borg El-Arab City Alexandria 21934 Egypt

This research aimed to optimize, purify, and biochemically and structurally characterize an acid-stable and organic solvent-tolerant lipase from the fungus Monascus pilosus . Sequence alignment indicated that the fungus in this study exhibits a high similarity of 99.63 % with M. pilosus (NR 163510.1). Maximum lipase production (434.3 U/mL) was observed at pH 3 after five days of incubation at 30 °C. The structure analysis showed that the lipase belongs to the GXSXL family, and the Ser163, Asp224, and His250 residues construct the catalytic triad. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) estimated the molecular weight of lipase to be around 51 kDa. Maximum lipase activity was detected at pH 8 and 50 °C, recording an activity of 625.3 U/mL. This lipase is remarkably stable across a pH range of 1.5 to 9, with a 1.8-fold activity enhancement after 2-h of incubation at pH 1.5. The enzyme displayed considerable stability in chloroform, hexane, toluene, and dimethyl sulfoxide (DMSO), retaining over 85 % residual activity at a 10 % concentration. This novel acid and organic solvent-tolerant lipase can provide a sustainable solution for industrial detergent formulation and other environmentally friendly applications. This is the first report to isolate, purify, and characterize lipase from the genus Monascus .

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Characterization of metabolic and imaging profiles in the diagnosis of succinate dehydrogenase- related hereditary pheochromocytomas and paragangliomas

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Urologic Oncology: Seminars and Original Investigations 2025年第6期43卷 393.e19-393.e25页

作者： Hsueh, Jessica Y. Mendhiratta, Neil Solomon, Julie Miller, Matthew Ryan, Beth Ricketts, Christopher J. Vocke, Cathy D. Crooks, Daniel R. Schmidt, Laura S. Merino, Maria Malayeri, Ashkan A. Linehan, W. Marston Ball, Mark W. Urologic Oncology Branch National Cancer Institute National Institutes of Health Bethesda MD United States Basic Science Program Frederick National Laboratory for Cancer Research Frederick MD United States Laboratory of Pathology Center for Cancer Research National Cancer Institute National Institutes of Health Bethesda MD United States Radiology and Imaging Sciences Clinical Center National Institutes of Health Bethesda MD United States

Purpose: Hereditary pheochromocytomas and paragangliomas can be associated with mutations in the mitochondrial enzyme succinate dehydrogenase. Due to their heterogenous clinical presentation, no clear universal screening guidelines are currently available. We sought to examine the utility of metabolic testing and imaging in diagnosing hereditary pheochromocytomas and paragangliomas. Materials and Methods: Our retrospective study included patients with confirmed familial succinate dehydrogenase mutations and histologically proven pheochromocytomas/paragangliomas or with suggestive metabolic and imaging features. We extracted information on age, race, sex, tumor size, tumor location, presence of metastatic disease and other succinate dehydrogenase-associated manifestations, urine and plasma metabolic testing, and diagnostic imaging. Patients who lacked any diagnostic information were excluded. Results: Our cohort consisted of 9 patients with 13 tumor occurrences from 2003 to 2023. The average age at diagnosis was 31.7 years with a 2.9 cm average tumor size. Three patients developed metastatic disease. Five tumors (38.5%) were biochemically silent, with all tumors detected on imaging. Most of the remaining tumors had a noradrenergic profile, with positive norepinephrine and normetanephrine in plasma and urine. I-123 MIBG was the least sensitive (50%) imaging modality, and Ga-68 DOTATATE PET/CT was the most sensitive (100%). 10 (76.9%) tumors were treated with surgical resection;all metabolic results were subsequently negative, except in patients with metastatic disease. Conclusions: Our investigation adds to the current literature on diagnosing hereditary pheochromocytomas and paragangliomas. We highlight the importance of multimodal screening that consists of both imaging and metabolic screening, especially given the prevalence of biochemically silent tumors. © 2025

关键词： Paraganglioma Pheochromocytoma Succinate dehydrogenase

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Large Negative Magnetoresistance in Antiferromagnetic Gd2Se3

arXiv

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arXiv 2025年

作者： Chhetri, Santosh Karki Acharya, Gokul Graf, David Basnet, Rabindra Rahman, Sumaya Sharma, M.M. Upreti, Dinesh Nabi, Md. Rafique Un Kryvyi, Serhii Sakon, Josh Mortazavi, Mansour Da, Bo Churchill, Hugh Hu, Jin Department of Physics University of Arkansas FayettevilleAR72701 United States National High Magnetic Field Lab TallahasseeFL32310 United States Department of Chemistry & Physics University of Arkansas at Pine Bluff Pine BluffAR71603 United States Material Science and Engineering Program Institute for Nanoscience and Engineering University of Arkansas FayettevilleAR72701 United States Department of Chemistry & Biochemistry University of Arkansas FayettevilleAR72701 United States MonArk NSF Quantum Foundry University of Arkansas FayettevilleAR United States Center for Basic Research on Materials National Institute for Materials Science Ibaraki Tsukuba305-0044 Japan

Rare earth chalcogenides provide a great platform to study exotic quantum phenomena such as superconductivity and charge density waves. Among various interesting properties, the coupling between magnetism and electronic transport has attracted significant attention. Here, we report the investigation of such coupling in α-Gd2Se3 single crystals through magnetic, calorimetric, and transport property measurements. α-Gd2Se3 is found to display an antiferromagnetic ground state below 11 K with metamagnetic spin-flop transitions. The magnetic fluctuations remain strong above the transition temperature. Transport measurements reveal an overall metallic transport behavior with a large negative magnetoresistance of ~ 65% near the magnetic transition temperature, together with positive MR near the field-induced spin-flop transitions, which can be understood in terms of the suppression of spin scattering by the magnetic field. © 2025, CC BY-NC-ND.

关键词： Gadolinium compounds

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Recent advances in electronic devices for monitoring and modulation of brain

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Nano Research 2021年第9期14卷 3070-3095页

作者： Yong Won Kwon Yoon Sun Jun Young-Geun Park Jiuk Jang Jang-Ung Park Nano Science Technology Institute Department of Materials Science and EngineeringYonsei UniversitySeoul 03722Republic of Korea Center for Nanomedicine Institute for Basic Science(IBS)Seoul 03722Republic of Korea Graduate Program of Nano Biomedical Engineering(NanoBME) Advanced Science InstituteYonsei UniversitySeoul 03722Republic of Korea

The brain is actuated by billions of neurons with trillions of interconnections that regulate human *** the mechanisms of these systems that induce sensory reactions and respond to disease remains one of the greatest challenges in science,engineering,and *** advances in nanomaterials and nanotechnologies have led to the extensive research of electronic devices for brain interfaces to better understand the neural activities of the brains complex nervous *** development of sensor devices for monitoring the physiological signals of the brain related to traumatic injury status has accompanied by the progress of electronic neural probes in *** addition,these neurological and stereotactic surgical revolutions hold immense potential for clinical analysis of pharmacological systems within cerebral ***,we review the progress of electronic devices interfacing with brain in terms of the materials,fabrication technologies,and device *** activity can be measured and modulated by brain probes based on newly developed nanofabrication ***,in vivo pathological monitoring of the brain and pharmacological assessment has been developed in miniaturized and wireless *** also consider the key challenges and prospects for further development,and explore the future directions emerging in the latest research.

关键词： neural interfaces neural recording neuromodulation intracranial pressure monitoring microfluidic drug delivery

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Genetics and genomics of prostate cancer

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Asian Journal of Andrology 2013年第3期15卷 309-313页

作者： Michael Dean Hong LOU Cancer and Inflammation Program National Cancer Institute National Institutes of Health Frederick MD 21702 USA Basic Science Program SAIC-Frederick Nationa Cancer Institute National Institutes of Health Frederick MD 21702 USA

Prostate cancer （PCa） is one of the most common malignancies in the world with over 890 000 cases and over 258 000 deaths worldwide each year. Nearly all mortalities from PCa are due to metastatic disease, typically through tumors that evolve to be hormone-refractory or castrate-resistant. Despite intensive epidemiological study, there are few known environmental risk factors, and age and family history are the major determinants. However, there is extreme heterogeneity in PCa incidence worldwide, suggesting that major determining factors have not been described. Genome-wide association studies have been performed and a considerable number of significant, but low-risk loci have been identified. In addition, several groups have analyzed PCa by determination of genomic copy number, fusion gene generation and targeted resequencing of candidate genes, as well as exome and whole genome sequencing. These initial studies have examined both primary and metastatic tumors as well as murine xenografts and identified somatic alterations in TP53and other potential driver genes, and the disturbance of androgen response and cell cycle pathways. It is hoped that continued characterization of risk factors as well as gene mutation and misregulation in tumors will aid in understanding, diagnosing and better treating PCa.

关键词： androgen receptor cancer progression chromatin remodeling metastasis prostate cancer （PCa） risk factor somaticmutation tumor heterogeneity

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Diameters of single-walled carbon nanotubes (SWCNTs) and related nanochemistry and nanobiology

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中国高等学校学术文摘·材料科学 2010年第1期4卷 17-28页

作者： Jie MA Jian-Nong WANG Chung-Jung TSAI Ruth NUSSINOV Buyong MA Shanghai Key Laboratory for Laser Processing and Materials Modification School of Materials Science and EngineeringShanghai Jiao Tong University Shanghai 200240 China Shanghai Key Laboratory for Metallic Functional Materials Key Laboratory for Advanced Civil Engineering Materials Ministry of EducationSchool of Materials Science and Engineering Tongji University Basic Science Program SAIC-Frederick Inc. Center for Cancer Research Nanobiology Program NCI-Frederiek NIH Frederick MD 21702 USA Basic Science Program SAIC-Frederick Inc. Center for Cancer Research Nanobiology Program NCI-Frederiek NIH Frederick MD 21702 USA Sackler Institute of Molecular Medicine Department of Human G

We reviewed and examined recent progresses related to the nanochemistry and nanobiology of signal-walled carbon nanotubes (SWCNTs), focusing on the diameters of SWCNTs and how the diameters affect the interactions of SWCNT with protein and DNA, which underlay more complex biological responses. The diameters of SWCNTs are closely related to the electronic structure and surface chemistry of SWCNTs, and subsequently affect the interaction of SWCNTs with membrane,protein, and DNA. The surfaces of SWCNT with smaller diameters are more polar, and these with large diameters are more hydrophobic. The preference of SWCNT to interact with Trp/Phe/Met residues indicates it is possible that SWCNT may interfere with normal protein-protein interactions. SWCNT-DNA interactions often change DNA conformation. Besides the promising future of using SWCNTs as delivering nanomaterial, thermal therapy, and other biological applications, we should thoroughly examine the possible effects of carbon nanotube on interrupting normal protein-protein interaction network and other genetic effects at the cellular level.

关键词： carbon nanotube (CNT) nanobiology protein DNA toxicity cancer

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Experimental test of an extension of the Rosenzweig-Porter model to mixed integrable-chaotic systems experiencing time-reversal invariance violation

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Chinese Physics B 2024年第12期33卷 203-213页

作者： Xiaodong Zhang Jiongning Che Barbara Dietz Lanzhou Center for Theoretical Physics Key Laboratory of Theoretical Physics of Gansu Provinceand Key Laboratory of Quantum Theory and Applications of the Ministry of EducationLanzhou UniversityLanzhou 730000China Center for Theoretical Physics of Complex Systems Institute for Basic Science(IBS)Daejeon 34126Korea Yangtze Delta Region Institute University of Electronic Science and Technology of ChinaHuzhou 313001China Basic Science Program Korea University of Science and Technology(UST)Daejeon 34113Korea

We report on the theoretical and experimental investigations of the transition of a typical quantum system with mixed regular-integrable classical dynamics to one with violated time-reversal(T)*** measurements are performed with a flat superconducting microwave resonator with circular shape in which chaoticity is induced by using either long antennas or inserting two circular disks into the cavity,and by magnetizing a ferrite disk placed at its center,which leads to violation of T *** propose an extension of the Rosenzweig-Porter(RP)model,which interpolates between mixed regular-chaotic instead of integrable dynamics and fully chaotic dynamics with violated T-invariance,and derive a Wigner-surmise like analytical expression for the corresponding nearest-neighbor spacing *** propose a procedure involving this result and those for the RP model to determine the size of T-invariance violation and chaoticity and validate it employing the experimental eigenfrequency spectra.

关键词： wave chaos quantum chaos quantum billiards microwave billiards random matrix theory microwave experiments

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Many-body flatband localization

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Physical Review B 2020年第4期102卷 041116(R)-041116(R)页

作者： Carlo Danieli Alexei Andreanov Sergej Flach Max Planck Institute for the Physics of Complex Systems Dresden D-01187 Germany Center for Theoretical Physics of Complex Systems Institute for Basic Science (IBS) Daejeon 34126 Korea Basic Science Program (IBS School) Korea University of Science and Technology (UST) Daejeon 34113 Korea New Zealand Institute for Advanced Study Massey University Auckland Private Bag 102904 0632 Auckland New Zealand

We generate translationally invariant systems exhibiting many-body localization from all-bands-flat single-particle lattice Hamiltonians dressed with suitable short-range many-body interactions. This phenomenon, dubbed many-body flatband localization, is based on symmetries of both single-particle and interaction terms in the Hamiltonian, and it holds for any interaction strength. We propose a generator of corresponding Hamiltonians which covers both interacting bosons and fermions for arbitrary lattice dimensions, and we provide explicit examples of such models in one and two lattice dimensions. We also explicitly construct an extensive set of local integrals of motion for this set of models. Our results can be further generalized to long-range interactions as well as to systems lacking translational invariance.

关键词： Many-body localization Disordered systems Hamiltonian systems

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The gut-skin axis in psoriasis: Evidence-based insights from a meta-analysis on probiotics-synbiotics-mediated microbiota interventions

Medicine in Microecology

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Medicine in Microecology 2025年 25卷

作者： Amalia, Nurlinah Wicaksono, Danar Wiyarta, Elvan Rampengan, Derren David Christian Homenta Darmawan, Hari Aditya, Muhammad Reva Athallah, Ariq Fadhil Cahyono, Maulana Bagus Adi Alatas, Aiman Idrus Tallei, Trina Ekawati Tjandrawinata, Raymond Rubianto Bhunia, Arun K. Nurkolis, Fahrul Medical Study Program Faculty of Medicine Universitas Brawijaya East Java Malang Indonesia Master Program of Biomedical Science Faculty of Medicine Universitas Brawijaya East Java Malang Indonesia Medical Research Center of Indonesia East Java Surabaya Indonesia Alumnus Department of Dermatology and Venereology Faculty of Medicine Public Health and Nursing Universitas Gadjah Mada Yogyakarta Indonesia University of Indonesia Hospital Depok 16424 Indonesia Risetku Jakarta South Jakarta 12820 Indonesia Medical Study Program Faculty of Medicine Universitas Sam Ratulangi North Sulawesi Manado Indonesia Dermatovenerology Department Faculty of Medicine Universitas Tarumanagara Jakarta Indonesia Medical Study Program Faculty of Medicine Universitas Airlangga East Java Surabaya Indonesia Program of Clinical Microbiology Residency Faculty of Medicine University of Indonesia Jakarta Indonesia Department of Biology Faculty of Mathematics and Natural Sciences Sam Ratulangi University Manado 95115 Indonesia Center for Pharmaceutical and Nutraceutical Research and Policy Faculty of Biotechnology Atma Jaya Catholic University of Indonesia Jakarta 12930 Indonesia Molecular Food Microbiology Laboratory Department of Food Science Purdue University West Lafayette 47907 IN United States Purdue Institute of Inflammation Immunology and Infectious Diseases Purdue University West Lafayette 47907 IN United States Department of Comparative Pathobiology Purdue University West Lafayette 47907 IN United States Master of Basic Medical Science Faculty of Medicine Universitas Airlangga Surabaya Indonesia State Islamic University of Sunan Kalijaga (UIN Sunan Kalijaga) Yogyakarta 55281 Indonesia

Psoriasis, a chronic immune-mediated skin condition, has been intricately linked to gut microbiota dysbiosis through the gut-skin axis. This meta-analysis synthesizes data from 15 randomized controlled trials encompassing 1,423 participants to evaluate the efficacy of gut microbiota interventions—probiotics and synbiotics—in psoriasis management. The findings reveal that probiotics significantly improved the Psoriasis Area and Severity Index (PASI) (Mean Difference [MD]: −4.05, 95 % CI: −6.73 to −1.38;p < 0.0001) and Dermatology Life Quality Index (DLQI) (MD: −5.74, 95 % CI: −11.45 to −0.03;p = 0.0001), outperforming synbiotics and systemic pharmacological therapies such as anti-TNF-α and anti-interleukin agents. Notably, probiotics demonstrated superior systemic anti-inflammatory (TNF-α and IL-17) and immunomodulatory responses, and enhanced gut barrier integrity. This study highlights probiotics as a promising adjunct or alternative therapy, paving the way for integrative treatment strategies that address psoriasis's multifaceted pathophysiology. Future research should focus on long-term efficacy and molecular mechanisms to optimize outcomes. These findings could redefine therapeutic paradigms, offering a cost-effective and accessible solution for millions of psoriasis patients worldwide. © 2025 The Authors

关键词： Gut microbiota Gut-skin axis Immune modulation Probiotics Psoriasis Synbiotics Systemic inflammation

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Unification certification criteria for professional engineers case study in Colombia, United States, Canada, Guatemala, Honduras, El Salvador

Unification certification criteria for professional engineer...

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2011 IEEE International Conference on Quality and Reliability, ICQR 2011

作者： Bernal, O. A. Vásquez National Distance Open University Bogotá Colombia School of Basic Science Technology and Engineering Industrial Engineer Program Colombia

ISBN: (纸本)9781457706288

Free trade agreements signed and under negotiation between Colombia and the countries of North and Central America, aimed at reducing and eliminating barriers to free movement of professionals to develop businesses that generate profits for each of the parties, this requires the security professional meets minimum requirements in line with international standards. The recognition of certifications, licenses among countries with respect to education and professional experience, standards of conduct and professional ethics is essential to ensure free mobility. This paper aims to develop the document review to unify criteria for certification and recertification of engineering and the impact of the free trade agreements concluded between Colombia, United States, Canada, El Salvador, Guatemala and Honduras. © 2011 IEEE.

关键词： Personnel training

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