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17th Annual Conference on Foundations of Nanoscience: Self-Assembled Architectures and Devices, FNANO 2020

作者： Zhao, Mengyu Chen, Yahong Wang, Kexin Streit, Jason K. Fagan, Jeffery A. Tang, Jianshi Zheng, Ming Zhu, Zhi Yang, Chaoyong James Sun, Wei Department of Electronics MOE Key Laboratory for Physics and Chemistry of Nanodevices Peking University Beijing100871 China Collaborative Innovation Center of Chemistry for Energy Materials The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation College of Chemistry and Chemical Engineering Xiamen University Xiamen361005 China Material Science and Engineering Division National Institute of Standards and Technology 100 Bureau Drive GaithersburgMD20899-8542 United States Beijing National Research Center for Information Science and Technology Institute of Microelectronics Tsinghua University Beijing100084 China

Structural DNA nanotechnology encodes the spatial positioning information into the linear sequences of composing single-stranded DNA. Arbitrary geometries, such as tube, cuboid, and polyhedron, could thus be in silico designed and scalable fabricated. The dimensions of self-assembled DNA structures have been scaled up from nanometer to micrometer. Diverse functional nano-materials, including gold nanoparticles, quantum dots, CNTs and oxides, have been assembled onto self-assembled DNA template. However, building highperformance electronics from self-assembled DNA templates is still difficult, owing to the presence of contaminations from bio-lattices and limited dimension scalability. © FNANO 2020 .All rights reserved.

关键词： Semiconductor quantum dots

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Harvesting of Microcystis Flos-Aquae Using Dissolved Air Flotation: The Inhibitory Effect of Carboxyl Groups in Uronic Acid-Containing Carbohydrates

SSRN

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SSRN 2022年

作者： Zhang, Xuezhi Cheng, Shaozhe Zhang, Haiyang Li, Lili Yu, Tongbo Wang, Yongpeng Tan, Daoyong Institute of Hydrobiology Chinese Academy of Sciences South Donghu Road Wuchang District Hubei Province Wuhan430072 China Yuquan Road Shijingshan District Beijing100049 China Key Laboratory of Solid Waste Treatment and Resource Recycle Ministry of Education Southwest University of Science and Technology Sichuan Mianyang621010 China

Harvesting algal biomass reduces nutrients loading in eutrophicated lakes and the protein rich microalgal biomass could be recycled as feedstocks of feed and fertilizer. Due to the complexity of algogenic organic matter (AOM), the key components and functional groups in AOM that inhibit coagulation based microalgal harvesting have not been disclosed thus far. This study quantitatively analyzed the responsive compositions and functional groups of AOM involved in the dissolved air flotation (DAF) harvesting of M. flos-aquae . The results showed that harvesting efficiency significantly deteriorated in the presence of AOM. Stoichiometric analysis of functional groups in carbohydrates, proteins and humic-like substances using model components revealed that carboxyl groups in uronic acids of carbohydrates were the major inhibitors. Carboxyl groups in uronic acid-containing carbohydrates accounted for 76% of the total reduction in carboxyl groups, which was much higher than that in proteins (23%) and humic-like substances (1%). A conceptual model of charge competition was proposed to explain the inhibition mechanism of carboxyl functional groups in uronic acid-containing carbohydrates on microalgal DAF. Strategies such as preventing carboxyl deprotonation by pH reduction and employment of sweeping/bridging polymeric coagulants/flocculants were proposed for the to reduce the inhibitory effect of carboxyl functional groups. © 2022, The Authors. All rights reserved.

关键词： Harvesting

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Quality Evaluation and Origin Traceability of the Imported and Domestic Saffron Spice (Crocus Sativus L.) Products in China Market Using Chemical Composition and Stable Isotope analysis

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SSRN 2022年

作者： Liu, Zhi Gao, Xianmei Yang, Jian Zhao, Duoyong Li, Bin Zhang, Zihong Chen, Piao Wang, Dongguang College of Agriculture and Biotechnology Hunan University of Humanities Science and Technology Loudi417000 China State Key Laboratory Breeding Base of Dao-di Herbs National Resource Center for Chinese Materia Medica China Academy of Chinese Medical Sciences Beijing100700 China Agricultural Products Quality and Safety Risk Assessment Laboratory Ministry of Agriculture and Rural Affairs Urumqi830091 China Agriculture and Biotechnology Innovation and Entrepreneurship Education Center Hunan University of Humanities Science and Technology Loudi417000 China

Saffron spice, as a high value-added but not high-tech processed agricultural product, is easily faked and mislabeled its quality grade and origin for higher market profits and competitiveness. In this work, an analytical strategy combining chemical composition analysis and stable isotope ratio analysis of saffron spices was explored for verifying their quality and origin authenticity in China market. The crocins contents and stable isotope ratios of 70 saffron spice samples collected from five provinces of China and 95 imported products from Iran and Spain were determined together. The contents of crocins may be effective as the indexes of their quality evaluation but not for origin traceability. Relatively, regional difference of stable isotope ratios is significant (p © 2022, The Authors. All rights reserved.

关键词： Agricultural products

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Standard:Human intestinal cancer organoids

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Cell Regeneration 2023年第1期12卷 193-198页

作者： Hanqing Lin Yalong Wang Chunyan Cheng Yuxin Qian Jie Hao Zhen Zhang Weiqi Sheng Linhong Song Chu‑Xia Deng Bing Zhao Jiani Cao Lei Wang Liu Wang Lingmin Liang Wenli Kelly Chen Chunping Yu Zhijian Sun Yingying Yang Changlin Wang Yong Zhang Qiyuan Li Ka Li Aijin Ma Tongbiao Zhao Ye-Guang Chen Guoqiang Hua D1Med Technology(Shanghai)Inc Shanghai 201802China The State Key Laboratory of Membrane Biology Tsinghua‑Peking Center for Life SciencesSchool of Life SciencesTsinghua UniversityBeijing 100084China Guangzhou Laboratory Guangzhou 510005China Guangzhou Institutes of Biomedicine and Health Chinese Academy of SciencesGuangzhou 510530China Guangzhou Hua Yi Regeneration Technology Co. LtdHuangpu DistrictGuangzhou 510700China National Stem Cell Resource Center State Key Laboratory of Stem Cell and Reproductive BiologyInstitute for Stem Cell and RegenerationInstitute of ZoologyChinese Academy of SciencesBeijing 100101China Beijing Institute for Stem Cell and Regenerative Medicine Beijing 100101China University of Chinese Academy of Sciences Beijing 100049China Institute of Clinical Science Zhongshan HospitalFudan UniversityShanghai 200433China Department of Radiation Oncology and Cancer Institute Fudan University Shanghai Cancer Center Fudan UniversityShanghai 200032China Department of Oncology Shanghai Medical CollegeFudan UniversityShanghai 200032China Shanghai Key Laboratory of Radiation Oncology Shanghai 200032China Department of Pathology Fudan University Shanghai Cancer CenterShanghai 200032China Sichuan Academy of Medical Sciences&Sichuan Provincial People’s Hospital Chengdu 610072China Cancer Centre Faculty of Health SciencesUniversity of MacaoMacao 999078SARChina State Key Laboratory of Genetic Engineering School of Life SciencesFudan UniversityShanghai 200438China China Innovation Center of Roche Li Shi Zhen RoadPudongShanghai 201203China Eli Lilly and Company PudongShanghai 201203China K2 Oncology Co. LtdKeChuang StreetBeijing 100176China Qilu Pharmaceutical Co. LtdJinan 250104China China National Institute of Standardization Beijing 100191China Chinese Society for Stem Cell Research Shanghai 200032China HHLIFE Co. IncShenzhen 518040China China National GeneBank Shenzhen 518000China Beijing Technology and Business University Beijing 100048China School of Bas

Intestinal cancer is one of the most frequent and lethal types of *** intestinal cancer using organoids has emerged in the last *** intestinal cancer organoids are physiologically relevant in vitro models,which provides an unprecedented opportunity for fundamental and applied research in colorectal cancer.“Human intestinal cancer organoids”is the first set of guidelines on human intestinal organoids in China,jointly drafted and agreed by the experts from the Chinese Society for Cell Biology and its branch society:the Chinese Society for Stem Cell *** standard specifies terms and definitions,technical requirements,test methods for human intestinal cancer organoids,which apply to the production and quality control during the process of manufacturing and testing of human intestinal cancer *** was released by the Chinese Society for Cell Biology on 24 September *** hope that the publication of this standard will guide institutional establishment,acceptance and execution of proper practocal protocols,and accelerate the international standardization of human intestinal cancer organoids for clinical development and therapeutic applications.

关键词： Human jointly apply

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analysis of typhoon wind hazard in Shenzhen City by Monte-Carlo Simulation

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Journal of Oceanology and Limnology 2019年第6期37卷 1994-2013页

作者： GUO Yunxia HOU Yijun QI Peng Key Laboratory of Ocean Circulation and Waves Institute of OceanologyChinese Academy of SciencesQingdao 266071China University of Chinese Academy of Sciences Beijing 100049China Laboratory for Ocean and Climate Dynamics Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China

As one of the most serious natural disasters,many typhoons affect southeastern China every *** Shenzhen,a coastal city in southeast China as an example,we employed a Monte-Carlo simulation to generate a large number of virtual typhoons for wind hazard *** analyzing 67-year historical typhoons data from 1949 to 2015 using the Best Track Dataset for Tropical Cyclones over the Western North Pacific recorded by the Shanghai Typhoon Institute,China Meteorological Administration(CMASTI),typhoon characteristic parameters were extracted and optimal statistical distributions established for the parameters in relation to *** employed the Monte-Carlo method to sample each distribution to generate the characteristic parameters of virtual *** addition,the Yah Meng(YM)wind field model was introduced,and the sensitivity of the YM model to several parameters *** the YM wind field model,extreme wind speeds were extracted from the virtual *** extreme wind speeds for different return periods were predicted and compared with the current structural code to provide improved wind load information for wind-resistant structural design.

关键词： typhoon hazard analysis Monte-Carlo simulation wind field model extreme wind speed

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How Do Planetary Boundary Layer Schemes Perform in PM2.5-O3Composite Pollution in the beijing-Tianjin-Hebei (BTH)? An In-Depth Mechanism analysis From the Underlying Code

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Journal of Geophysical Research: Atmospheres 2025年第12期130卷

作者： Wenxing Jia Xiaoye Zhang Yi Lin Deying Wang Hong Wang Yaqiang Wang State Key Laboratory of Severe Weather Meteorological Science and Technology & Key Laboratory of Atmospheric Chemistry of CMA CAMS Beijing China Center for Excellence in Regional Atmospheric Environment IUE Chinese Academy of Sciences Xiamen China Key Laboratory for Mesoscale Severe Weather Ministry of Education and School of Atmospheric Sciences Nanjing University Nanjing China

Parameterization schemes undergo continuous updates, with most refinements documented in code implementations, necessitating in-depth code analysis for evaluation. Focusing on PM 2.5 -O 3 composite pollution in the beijing-Tianjin-Hebei region, 10 planetary boundary layer (PBL) schemes were evaluated for their simulation performance. The 2-m temperature ( T 2 ) demonstrates daytime dependence on temperature gradient and transport coefficient, shifting to surface temperature dominance at night. Saturated mixing ratio predominantly governs 2-m relative humidity ( RH 2 ) variations, while 10-m wind speed ( WS 10 ) derives from the first-level wind speed. Vertical profile comparisons reveal degraded simulation accuracy at lower levels, attributed to human activities’ influences within the PBL. The total energy-mass flux scheme exhibited distinct deviations due to failure to recognize the existence of shallow cumulus, consequently suppressing vertical mixing. Turbulent diffusion coefficient ( K ) discrepancies in nonlocal closure schemes primarily stem from PBL height (PBLH) variations, whereas local closure schemes show mixing length dominance (∼63%). Dissipation rate considerations exert stronger impacts at lower levels. For hybrid schemes, K calculated by Monin-Obukhov similarity theory shows enhanced magnitudes during daytime. Statistical analysis indicates the Mellor-Yamada-Janjić scheme's minor T 2 deficiencies become insignificant when weighed against its superior vertical profiles of T , RH , and WS 10 . These insights offer critical references for PBL scheme optimization. The total energy-mass flux scheme is special for not recognizing the presence of shallow cumulus clouds, resulting in little or no vertical mixing The turbulent diffusion coefficient is mainly influenced by the height of the planetary boundary layer (mixing length) for nonlocal (local) schemes The Mellor-Yamada-Janjić scheme is found to be the statistically optimal choice for PM 2.5 -O 3 compos

关键词： boundary layer schemes turbulent diffusion PM2.5-O3 composite pollution underlying code

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Smartphone-controlled Electrochemical Sensor for Copper Detection*

Smartphone-controlled Electrochemical Sensor for Copper Dete...

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IEEE International Conference on Nano/Micro Engineered and Molecular Systems (NEMS)

作者： Yang Li Yu Song Jianhua Tong Chao Bian Shanhong Xia Hanpeng Dong State Key Laboratory of Transducer Technology the Aerospace Information Research Institute (AIR) Chinese Academy of Sciences (CAS) Beijing China Key Laboratory of Sensor Beijing Information Science and Technology University Beijing China

ISBN: (数字)9781728172309

ISBN: (纸本)9781728172316

This paper demonstrated a smartphone- controlled electrochemical sensor for copper ions (Cu 2+ ) detection in waters. The electrochemical sensor is composed of a three-electrode-system and a detecting circuit module. The three-electrode-system is configured as an Au working- electrode, a Pt counter-electrode and an Ag/AgCl reference electrode. Configured under the square wave impulse voltammetry (SWV), the detecting circuit utilizes low-power consuming MCU to carry out constant potential control and weak current detection. Smartphone installed with the customized App can be used to control the operation of the sensor and implement data management in the detection process. The App was developed to realize the function of man-machine interaction and display testing curves of the electroanalytical measurements. Data exchange between the sensor and the smartphone is based on the Bluetooth protocol. A series of standard copper samples were tested to evaluate performance of the sensor, and the experimental results reveal that the sensor exhibits better sensitivity (4.064 μA/μgL -1 ) to Cu 2+ than the original prototype from 0 μg/L to 1000 μg/L.

关键词： Actuators Radio frequency Copper Sensor systems Iron Ions Conferences

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Novel Lockable Fluorescent Lateral Immunoassay Strip with Oriented Modified Antibody for the Rapid Detection of Acetamiprid in Vegetables

SSRN

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SSRN 2024年

作者： Li, Donghan Dong, Haowei Li, Zhengtao Wang, Haifang Sun, Jiashuai Huang, Jingcheng Li, Peisen Zhou, Shuxian Zhai, Shengxi Zhao, Mingxin Sun, Xia Guo, Yemin School of Agricultural Engineering and Food Science Shandong University of Technology No. 266 Xincun Xilu Shandong Zibo255049 China Shandong Provincial Engineering Research Center of Vegetable Safety and Quality Traceability No. 266 Xincun Xilu Shandong Zibo255049 China Zibo City Key Laboratory of Agricultural Product Safety Traceability No. 266 Xincun Xilu Shandong Zibo255049 China Dongzhimen Hospital Beijing University of Chinese Medicine Beijing100700 China Institute of Fruit and Floriculture of Gansu Academy of Agricultural Sciences Gansu Lanzhou730070 China

Time-resolved fluorescent lateral immunoassay strip (TRFLIS) is a reliable and rapid method for detecting acetamiprid (ACE). However, its sensitivity is often affected by structural patterns and stability of the probe. Herein, goat anti-mouse IgG (GaMIgG) was used as a sandwich protein to indirectly link acetamiprid antibody (AAb) to europium oxide time-resolved fluorescent microsphere (TRFM). This allowed for oriented modification of the AAb. Additionally, 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride was innovatively used as a cross-linking agent to enhance the binding of AAb to GaMIgG, which improved the stability of TRFM-GaMIgG-AAb. Under optimal working conditions, TRFLIS exhibited a wide linear response range of 5-700 ng/mL. Its limit of detection (LOD) was 0.62 ng/mL, and the visual LOD was 5 ng/mL. This strategy effectively reduced the dosage of AAb compared with TRFM directly linking AAb. Meanwhile, the TRFLIS showed outstanding specificity and accuracy in ACE detection and was successfully applied to vegetable sample testing. The TRFLIS enables rapid and accurate detection of large-volume samples by combining qualitative and quantitative methods. It has great potential for use in the development of low-cost and high-performance immunochromatographic platforms. © 2024, The Authors. All rights reserved.

关键词： Antibodies

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A Platform Service for Passenger Volume analysis on Massive Smart Card Data in Public Transportation Domain 15th

A Platform Service for Passenger Volume Analysis on Massive ...

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15th EAI International Conference on Collaborative Computing: Networking, Applications and Worksharing, CollaborateCom 2019

作者： Ding, Weilong Wang, Zhe Zhao, Zhuofeng School of Computer Science and Technology North China University of Technology Beijing100144 China Beijing Key Laboratory on Integration and Analysis of Large-Scale Stream Data Beijing100144 China

ISBN: (纸本)9783030301453

In current public transportation of modern cities, the passenger volume analysis counts the bus passengers in multiple perspectives, and it is significant to optimize the bus scheduling and evaluate transportation capacity. On the smart card data of passengers taking buses, traditional solutions have inherent limitations about long processing delay, inaccuracy result and poor scalability. In this paper, the spatio-temporal correlation with business restrictions is considered, and an effective platform service for passenger volumes analyses are proposed on massive smart card. Our service has been applied in practical usage for three types of passenger volume, and holds minute-level latencies on weekly data with nearly linear scalability in extensive conditions. © 2019, ICST Institute for Computer sciences, Social Informatics and Telecommunications Engineering.

关键词： Smart cards

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L-proline feeding for augmented freeze tolerance of Camponotus japonicus Mayr

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science Bulletin 2019年第23期64卷 1795-1804页

作者： Mengjia Dou Yazhou Li Ziqiao Sun Lei Lia Wei Rao C4S Key Laboratory of Cryogenics Technical Institute of Physics and ChemistryChinese Academy of SciencesBeijing 100190China School of Engineering Science University of Chinese Academy of SciencesBeijing 100049China School of Energy and Power Engineering Beihang UniversityBeijing 100191China Beijing Engineering Research Center of Sustainable Energy and Buildings Beijing University of Civil Engineering and ArchitectureBeijing 100044China School of Future Technology University of Chinese Academy of SciencesBeijing 100049China Beijing Key Laboratory of Cryo-Biomedical Engineering Beijing 100190China

The successful cryopreservation of organs is a strong and widespread demand around the world but faces great challenges. The mechanisms of cold tolerance of organisms in nature inspirit researchers to find new solutions for these challenges. Especially, the thermal, mechanical, biological and biophysical changes during the regulation of freezing tolerance process should be studied and coordinated to improve the cryopreservation technique and quality of complex organs. Here the cold tolerance of the Japanese carpenter ants, Camponotus japonicus Mayr, was greatly improved by using optimal protocols and feeding on L-proline-augmented diets for 5 days. When cooling to -27.66 ℃, the survival rate of frozen ants increased from 37.50% ± 1.73% to 83.88% ± 3.67%. Profiling of metabolites identified the concentration of whole-body L-proline of ants increased from 1.78 to 4.64 ng g-1 after 5-day feeding. High Lproline level, together with a low rate of osmotically active water and osmotically inactive water facilitated the prevention of cryoinjury. More importantly, gene analysis showed that the expression of ribosome genes was significantly up-regulated and played an important role in manipulating freezing tolerance. To the best of our knowledge, this is the first study to link genetic variation to the enhancement of ants’ cold tolerance by feeding exogenous cryoprotective compound. It is worth noting that the findings provide the theoretical and technical foundation for the cryopreservation of more complex tissues,organs, and living organisms.

关键词： L-proline Cryopreservation Freeze tolerance Metabolomics Gene expression

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