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Allele-specific genome editing of imprinting genes by preferentially targeting non-methylated loci using Staphylococcus aureus Cas9(SaCas9)

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science Bulletin 2019年第21期64卷 1592-1600页

作者： Yajing Liu Jianan Li Changyang Zhou Bin Meng Yu Wei Guang Yang Zongyang Lu Qingmei Shen Yu Zhang Hui Yang Yunbo Qiao School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China Institute of Biochemistry and Cell Biology Chinese Academy of SciencesShanghai 200031China University of Chinese Academy of Sciences Beijing 100049China Institute of Neuroscience State Key Laboratory of NeuroscienceKey Laboratory of Primate NeurobiologyCAS Center for Excellence in Brain Science and Intelligence TechnologyShanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200031China Shanghai Institute for Advanced Immunochemical Studies ShanghaiTech UniversityShanghai 200031China Precise Genome Engineering Center School of Life SciencesCuangzhou UniversityGuangzhou 510006China

Allele-specific DNA methylation is the most important imprinting marker localized to differentially methylated regions(DMRs),and aberrant genomic imprinted DNA methylation is associated with some human diseases,including Prader-Willi syndrome and ***,the development of an effective strategy for the precise editing of allele-specific methylated genes is essential for the functional clarification of imprinting elements and the correction of imprinting disorders in human *** discover a feasible allele-specific genome editing tool based on the CRISPR/Cas system,which is an efficient genetargeting technique in various organisms,we examined the targeting efficiency of Staphylococcus aureus Cas9(SaCas9)and Streptococcus pyogenes Cas9(SpCas9)in response to DNA methylation *** found that the targeting efficiency of SaCas9,but not SpCas9,was enhanced by targeted DNA demethylation using the d Cas9-Tet1 catalytic domain(CD)but suppressed by targeted DNA methylation using Dnmt3l-Dnmt3a-d *** in vitro cleavage assay further demonstrated that SaCas9 nuclease activity was inhibited by 5-methylcytosine(5mC)in a synthesized Cp G-containing *** analysis with Ch IP-Q-PCR demonstrated that the non-methylated sequence targeting of Sa Cas9 depends on the binding preference of SaCas9 to non-methylated *** advantage of this feature of SaCas9,we have successfully obtained non-methylated allele-biased targeted embryos/mice for two imprinting genes,H19 and Snrpn,with relatively high efficiencies of 28.6%and 47.4%,*** results indicate that the targeting efficiency of SaCas9 was strongly reduced by DNA *** using SaCas9,we successfully achieved allele-specific genome editing of imprinting genes by preferentially targeting non-methylated loci.

关键词： Allele-specific DNA methylation SaCas9 Imprinting gene

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Highly oxygenated acorane sesquiterpenes from the marine-sourced Trichoderma harzianum CS-152

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Fitoterapia 2025年 185卷 106672页

作者： Yan-He Li Xiao-Ming Li Xin Li Xiao-Shan Shi Sui-Qun Yang Hong-Lei Li Bin-Gui Wang CAS and Shandong Province Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences Nanhai Road 7 Qingdao 266071 China Laboratory for Marine Biology and Biotechnology Qingdao Marine Science and Technology Center Wenhai Road 1 Qingdao 266237 China University of Chinese Academy of Sciences Yuquan Road 19A Beijing 100049 China. Laboratory for Marine Biology and Biotechnology Qingdao Marine Science and Technology Center Wenhai Road 1 Qingdao 266237 China. Laboratory for Marine Biology and Biotechnology Qingdao Marine Science and Technology Center Wenhai Road 1 Qingdao 266237 China. Electronic address: lihonglei@***.

Three new highly oxygenated acorane sesquiterpenes, namely, 9,10-epoxidicacoren-2,7,8-triol (1), 8S-acoren-2,7,8-triol (2), and 8R-acoren-2,7,8-triol (3), along with four known analogs (4-7), were isolated from the deep-sea derived fungus Trichoderma harzianum CS-152. Except for compound 7, the other compounds lack conjugation and do not exhibit fluorescence under UV light at 254 nm, which posed a challenge in their detection and required careful monitoring during separation. The isolation and purification of these compounds was achieved using traditional silica gel column chromatography. Their chemical structures and relative configurations were elucidated by comprehensive analysis of 1D/2D NMR, HRESIMS, and their absolute configurations were determined by modified Mosher's method. Compounds 1 and 4 displayed inhibitory activities against the aquatic pathogen Aeromonas hydrophila, each with a MIC value 4 μg/mL, while compound 7 showed antibacterial activity against Pseudomonas aeruginosa with a MIC value 4 μg/mL.

关键词： Acorane sesquiterpenes Antibacterial activity Cold-seep Deep sea-derived fungus Trichoderma harzianum

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Enhanced exchange bias in all-oxide heterostructures with cation-ordered ferrimagnetic double-perovskite

npj Spintronics

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npj Spintronics 2024年第1期2卷 1-6页

作者： Xiaofu Qiu Hetian Chen Yuhan Liang Xiaoyu Jiang Jing Ma Di Yi Zelin Wang Tianxiang Nan Yujun Zhang Fangyuan Zhu Zhen Chen State Key Laboratory of New Ceramics and Fine Processing School of Materials Science and Engineering Tsinghua University Beijing 100084 China School of Integrated Circuits Beijing National Research Center for Information Science and Technology Tsinghua University Beijing 100084 China National Center for Electron Microscopy in Beijing School of Materials Science and Engineering Tsinghua University Beijing 100084 China Institute of High Energy Physics Chinese Academy of Sciences Beijing 100049 China Shanghai Synchrotron Radiation Facility Shanghai Advanced Research Institute Chinese Academy of Sciences Shanghai 201204 China Beijing National Laboratory for Condensed Matter Physics Institute of Physics Chinese Academy of Sciences Beijing 100190 China School of Physical Sciences University of Chinese Academy of Sciences Beijing 100049 China

The realization and control of exchange bias (EB) are highly desirable for spintronic applications. All-oxide heterostructures comprised of ferromagnetic and antiferromagnetic/multiferroic oxides provide an ideal platform to enable the electric-field control of EB, promising for energy-efficient memory and logic devices. However, the low block temperature (TB) and small bias field (HEB) hinder further advances towards room-temperature applications. Here, we report an alternative approach to enhance the interface-induced EB by using ferrimagnetic double-perovskite with B-site cation ordering. In heterostructures comprised of double-perovskite Sr2FeReO6(SFRO) and LaFeO3(LFO), a high TB(about 250 K) and large HEBare observed, which is significantly larger than the counterparts with LFO and ferromagnetic oxides. Further analysis suggests that the cation-ordering and ferrimagnetic spin structure of the double-perovskite could contribute significantly to the enhanced exchanged bias when interfacing with G-type antiferromagnets. Our results open a new avenue for developing all-oxides heterostructures for future magnetic technologies.

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In-situ recycling of waste ozonation catalysts into N-doped graphitic carbon-alumina structures induces a non-radical pathway towards water purification

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Water Research 2025年

作者： Min Li Xiaoguang Jin Zhouyang Li Yu Fan Yutang Zeng Liya Fu Changyong Wu College of Water Sciences Beijing Normal University Beijing 100875 China Research Center of Water Pollution Control Technology Chinese Research Academy of Environment Sciences Beijing 100012 China Sinopec Research Institute of Petroleum Engineering Co. Ltd Beijing 102206 China Key Laboratory of Environmental Biotechnology Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China Department of Environmental Science and Engineering Fudan University Shanghai 200433 China State Key Laboratory for Ecological Security of Regions and Cities Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China

With the widespread application of catalytic ozonation in the advanced treatment of industrial wastewater, the increasing of waste ozonation catalysts has posed substantial environmental and economic challenges. Although waste catalysts represent an attractive resource, environmentally friendly and efficient recycling strategies remain elusive due to their complex structures and compositions. Herein, we propose a one-step pyrolysis strategy to ingeniously recycle waste catalysts into composite catalysts (Cu-Mn/NGC-Al 2 O 3 ) for industrial wastewater treatment. This procedure involves the in-situ graphitization of extracellular polymeric substances from the waste catalysts onto the pristine Al 2 O 3 substrates, and trace amounts of copper and manganese undergo redox-reactive recycling to form nanoparticles. Waste catalyst-derived Cu-Mn/NGC-Al 2 O 3 demonstrates recovery of 80.83% activity of the original fresh catalysts, along with excellent stability. Unlike the radical oxidation of fresh catalysts, we identify that Cu-Mn/NGC-Al 2 O 3 configuration induces a non-radical pathway based on singlet oxygen with a contribution of 84.09%. Life cycle assessment and economic analysis indicate that recycling waste catalysts to Cu-Mn/NGC-Al 2 O 3 achieves a 93.89% reduction in carbon emissions and saves approximately 58.58% in costs. This study promotes the recycling of waste catalysts and maximizes the utilization of resources from waste catalysts, achieving the goal of the low-carbon transition.

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Simultaneous optical g, r, i monitoring and IDV periodic analysis for quasar 3C 454.3

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Research in Astronomy and Astrophysics 2019年第10期19卷 43-50页

作者： Jun-Hui Fan Yu-Hai Yuan Hong Wu Feng Wang Jun Tao Min-Feng Gu Center for Astrophysics Guangzhou University Guangzhou 510006 China Astronomy Science and Technology Research Laboratory of Department of Education of Guangdong Province Guangzhou 510006 China Key Laboratory of Optical Astronomy National Astronomical Observatories Chinese Academy of Sciences Beijing 100101 China Shanghai Astronomical Observatory Chinese Academy of Sciences Shanghai 200030 China Key Laboratory for Research in Galaxies and Cosmology Shanghai Astronomical Observatory Chinese Academy of Sciences Shanghai 200030 China

With the 1.26 m National Astronomical Observatory-Guangzhou University Infrared/Optical Telescope(NAGIOT) at Xinglong Station of National Astronomical Observatories, Chinese academy of sciences, we obtained 419 groups of simultaneous observations at g, r and i bands, for the first time,targeting quasar 3 C 454.3 during 15 nights from 2016 October 23 to 2016 December 15. Based on our observations, we investigate the optical variabilities, the relation between brightness and color index, and the periodicity variability. The presented analyses demonstrate that: 1. The maximum variations at the g, r and i bands are △mg|max= 1.015 ± 0.042 mag,△mr|max= 1.188 ± 0.050 mag and △mi|max= 1.305 ± 0.057 mag respectively. 2. During our 15 night monitoring program, intra-day variability was detected on one night(Nov. 2). Also, the brightness increased by A = 15.86% over 50.8 min,then decreased by A = 22.42% over 40.1 min. After a small bright state, its brightness increased again by18.1% over 55 min at the g band. Similar phenomena happened at r and i bands. The intra-day variabilities at the three bands on 2016 November 2 indicate a period of 105 min, which implies a black hole mass of MBH =(0.3 ~ 1.85)× 10^9 M⊙. 3. There is an anti-correlation between color index and magnitude,suggesting the source becomes redder when it brightens.

关键词： galaxies quasars individual (3C 454.3)

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Notice of Violation of IEEE Publication Principles: Tone Mapping Beyond the Classical Receptive Field

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IEEE Transactions on Image Processing 2020年 29卷 4174-4187页

作者： Shao-Bing Gao Min-Jie Tan Zhen He Yong-Jie Li College of Computer Science Sichuan University Chengdu China School of Aeronautics and Astronautics Sichuan University Chengdu China Academy of Military Medical Sciences Beijing China MOE Key Laboratory for NeuroInformation School of Life Science and Technology University of Electronic Science and Technology of China Chengdu China

Notice of Violation of IEEE Publication Principles "Tone Mapping Beyond the Classical Receptive Field," by Shao-Bing Gao, Min-Jie Tan, Zhen He, and Yong-Jie Li in the IEEE Transactions on Image Processing, vol. 29, February 2020, pp. 4174???4187 After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE's Publication Principles. This paper contains significant portions of content from the paper cited below. The original content was copied without attribution (including appropriate references to the original author(s) and/or paper title) and without permission. Due to the nature of this violation, reasonable effort should be made to remove all past references to this paper, and future references should be made to the following paper "Colour Constancy Beyond the Classical Receptive Field," by Arash Akbarinia, and C. Alejandro Parraga, in the IEEE Transactions on Pattern analysis and Machine Intelligence, vol. 40, no. 9,September 2018, pp. 2081???2094 Some neurons in the primary visual cortex (V1) of human visual system (HVS) conduct dynamic center-surround computation, which is thought to contribute to compress the high dynamic range (HDR) scene and preserve the details. We simulate this dynamic receptive field (RF) property of V1 neurons to solve the so-called tone mapping (TM) task in this paper. The novelties of our method are as follows. (1) Cortical processing mechanisms of HVS are modeled to build a local TM operation based on two Gaussian functions whose kernels and weights adapt according to the center-surround contrast, thus reducing halo artifacts and effectively enhancing the local details of bright and dark parts of image. (2) Our method uses an adaptive filter that follows the contrast levels of the image, which is computationally very efficient. (3) The local fusion between the center and surround responses returned by a cortical processing flow and the glo

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Phase coexistence and evolution in sol-gel derived BY-PT-PZ ceramics with significantly enhanced piezoelectricity and high temperature stability

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Journal of Materiomics 2019年第3期5卷 394-403页

作者： Kai Cai Xue Yan Pingye Deng Li Jin Yang Bai Fei Zeng Dong Guo Beijing Center for Physical and Chemical Analysis Beijing100089China School of Materials Science and Engineering Beihang UniversityBeijing100191China Electronic Materials Research Laboratory School of Electronic and Information EngineeringXi'an Jiaotong UniversityXi'an710049China School of Materials Science and Engineering University of Science and Technology BeijngBeijing100083China School of Material Science and Engineering Tsinghua UniversityBeijing100084China Institute of Acoustics Chinese Academy of SciencesBeijing100190China

In this study,high Curie Temperature(T_(c))perovskite ceramics of optimized composition 0.55(0.1BiYbO_(3)-0.9PbTiO_(3))-0.45PbZrO_(3) with unique double orthorhombic main phases were prepared by a modified solgel *** to the usual solid-state prepared sample,the sol-gel derived sample has a 1.6 times higher d_(33) of 325 pC/N,a 2.4 times higher remnant polarization,and a much better high temperature stability with similar depolarization temperature(T_(d))and T_(c).Comprehensive analysis of the xerogel prepared over a wide calcination temperature(T_(cal))range of 300-1000℃ revealed that perovskite structure appeared at only 400℃ and it became the main phase above 500℃.Comparison of XRD refinement results showed that calcination and sintering induced subtle and continuous phase transition,namely,the 400-900℃ calcined powders with coexisted tetragonal(P4mm)and orthorhombic(Pbam)phase changed to a rather stable double orthorhombic(Pmmm and Pbam)main phase in all the differently sintered ceramics,as similar to the 1000C calcined *** stable phase coexistence well explains the enhanced *** results also demonstrate that optimized sol-gel processing can provide high T_(c) ceramics with desirable multi-phase structure and significantly enhanced performance at a lower temperature.

关键词： Piezoelectricity Curie temperature Phase transition Sol-gel Perovskite

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Deep learning based sferics recognition for AMT data processing in the dead band

arXiv

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arXiv 2022年

作者： Jiang, Enhua Chen, Rujun Wu, Xinming Liu, Jianxin Zhu, Debin Pitiya, Regean Liu, Weiqiang Changsha410083 China Key Laboratory of Non-ferrous Resources and Geological Hazard Detection Central South University Changsha410083 China School of Geoscience and Info-physics Central South University South Lushan Road Changsha410083 China School of Earth and Space Sciences University of Science and Technology of China Hefei230026 China Chinese Academy of Geological Sciences Beijing100037 China

The main signals from the field sources of audio-magnetotellurics (AMT) sounding generated by global lightning activities are called sferics. In data analysis, the absence of long-term and high-amplitude natural field signals will result in a lack of energy in the AMT dead-band, which may cause unreliable impedance estimate. The collected data itself is redundant, and most of the data segments are non-signal segments containing noise. To achieve reliable AMT data interpretation, professional and rigorous preprocessing methods such as cropping or selection are required. In order to efficiently extract AMT main signals, we propose a deep convolutional neural network (CNN) to automatically recognize sferic signals from time series. We train the CNN by using field time series data with different signal to noise rations (S/Ns) that were acquired from different regions in mainland China. To solve the potential overfitting problem due to the limited number of sferic labels, we propose a training strategy that randomly generates training samples (with random data augmentations) while optimizing the CNN model parameters. We stop the process of training and data generation until the training loss converges. In addition, we use a weighted binary cross-entropy loss function to solve the sample imbalance problem to better optimize the network, use multiple reasonable metrics to evaluate network performance, and carry out ablation experiments to optimally choose the model hyperparameters. Extensive field data applications show that our trained CNN can robustly recognize sferic signals from noisy time series for subsequent impedance estimation. The computed results show that our method can significantly improve S/N and effectively solve the problem of lack of energy in AMT dead-band. We obtained a smoother and more reasonable apparent resistivity-phase curves and depolarized phase tensor in the dead-band. corrected the estimation error of sudden drop/abrupt change of high-frequenc

关键词： Magnetotellurics

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Development of colorimetric immunochromatographic assay based on different morphologies of gold nanoparticles for the detection of triadimefon

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Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy 2025年 343卷 126544页

作者： Dong, Sa Zhang, Yu Shi, Qiuyun Zhu, Zixin Wang, Yulong Luo, Ruifeng Feng, Jianguo College of Plant Protection Yangzhou University Jiangsu Yangzhou 225009 China Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding Base Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality Ministry of Agriculture Institute of Food Safety and Nutrition Jiangsu Academy of Agricultural Sciences Jiangsu Nanjing 210014 China Food Quality Supervision and Testing Center of Ministry of Agriculture Xinjiang Academy of Agricultural and Reclamation Science Xinjiang Shihezi 832000 China

Triadimefon (TDF) is a highly effective, low toxicity, broad-spectrum triazole fungicide with strong systemic absorption. Excessive residues in tobacco and vegetables can affect human health. Therefore, it is urgent to establish a simple, rapid, and sensitive detection method for residual analysis of TDF in tobacco and vegetables. In this study, colloidal gold nanoparticles (Au NPs), gold nanoflowers (Au NFs), and gold nanorods (Au NRs) were synthesized and combined with anti-TDF monoclonal antibodies (mAb) to prepare probes for immunochromatographic strips (ICS). The lowest visual detection limits (vLODs) of the test strips using AuNPs-mAb, AuNFs-mAb, and AuNRs-mAb as probes were 7.8, 15.6, and 15.6 ng/mL, respectively. The three test strips showed no significant cross reactivity with the four structural analogues of tebuconazole, hexaconazole, myclobutanil, and difenoconazole, indicating that all three test strips have good specificity. To assess the presence of 0 and 62.5 ng/mL of TDF, ten test strips were used, and the results showed perfect accuracy with no false positives or false negatives. In addition, three test strips (same batch) were utilized to detect TDF (0 and 62.5 ng/mL) after storage for three months, and the results were still accurate, indicating that all three kinds of test strips have good repeatability and stability. The addition and recovery tests of TDF were conducted on cucumber and tobacco leaf samples (0, 2.5, 17.5, and 65 μg/kg), and the test results revealed that the strips performed with high accuracy. This study established three colorimetric test strips based on three different morphologies of gold nanoparticles, which possess the benefits of quick processing times, high cost-effectiveness, and minimal technical requirements, making them suitable for rapid on-site qualitative detection of TDF. © 2025 Elsevier B.V.

关键词： Gold nanoflowers Gold nanoparticles Gold nanorods Immunochromatographic strip Monoclonal antibody Triadimefon

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Effect of long-acting polyethylene glycol recombinant human growth hormone dosages in pediatric practice:A pooled analysis of two phase IV randomized controlled trials

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Chinese Medical Journal 2024年第17期137卷 2125-2127页

作者： Ling Hou Xuefeng Chen Hongwei Du Jianping Zhang Xinran Cheng Jiayan Pan Hui Pan Wei Liao Xiaoping Luo Junfen Fu on Behalf of the 045 Study Group Department of Pediatrics Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhanHubei 430030China Department of Endocrinology Children’s Hospital of Zhejiang University School of MedicineNational Clinical Research Center for Child HealthZhejiang University School of MedicineHangzhouZhejiang 310052China Department of Paediatrics First Hospital of Jilin UniversityChangchunJilin 130021China Department of Pediatrics Ningbo Women&Children’s HospitalNingboZhejiang 315012China Department of Pediatric Endocrine Genetics and Metabolism Chengdu Women’s and Children’s Center HospitalChengduSichuan 610074China Department of Pediatrics Wuhu First People’s HospitalWuhuAnhui 241000China Department of Endocrinology Key Laboratory of Endocrinology of National Health CommissionState Key Laboratory of Complex Severe and Rare DiseasesPeking Union Medical College HospitalChinese Academy of Medical Science and Peking Union Medical CollegeBeijing 100730China Department of Pediatrics The First Affiliated Hospital of Army Medical University(Third Military Medical University)Chongqing 400038China 不详

To the Editor:Growth hormone deficiency(GHD)impairs growth and development,affecting approximately 1 in 4000 children worldwide.[1]Recombinant human growth hormone(rhGH)has been standard practice since 1985.[2]However,compliance has been challenged by the need for frequent *** glycol rhGH(PEG-rhGH)was developed,improving protein stability and extending *** studies suggested that a weekly dosage of 0.20 mg/kg of PEG-rhGH offers improved growth rates and increased insulin-like growth factor-1 standard deviation score(IGF-1 SDS)without novel toxicities.[3]Here,we present a pooled analysis of two phase IV trials evaluating the efficacy and safety of various PEG-rhGH dosages in GHD.

关键词： dosage analysis glycol

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