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Rapid multiplex pathogen detection using 96-channel microfluidic chip with magnetic bead method

Chinese Chemical Letters 2025年

作者： Enjia Zhang Jiaying Cao Jianxin Cheng Gaozhe Cai Shuyue Jiang Weiwei Xie Chunping Jia Jianlong Zhao Shilun Feng College of Chemistry and Materials Science Shanghai Normal University Shanghai 200234 China State Key Laboratory of Transducer Technology Shanghai Institute of Microsystem and Information Technology Chinese Academy of Sciences Shanghai 200050 China 2020 X-Lab Shanghai Institute of Microsystem and Information Technology Chinese Academy of Sciences Shanghai 200050 China Xiangfu Laboratory Jiashan 314100 China School of Microelectronics Shanghai University Shanghai 200444 China Department of Pharmacy The Second Hospital of Hebei Medical University Shijiazhuang 050000 China Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of Sciences Beijing 100049 China

The implementation of multiple pathogen testing is essential for a rapid response to future outbreaks and for reducing disease transmission. This study introduces a 96-channel microfluidic chip, fabricated through a molding process, which enables the batch detection of pathogens. It explores the rapid lysis and elution processes of pathogens within the microfluidic chips to ensure that nucleic acid extraction, elution, and amplification are completed entirely within the chip. This chip can extract nucleic acids from samples in just 10 min, achieving an extraction efficiency comparable to that of traditional in-tube methods. An oil phase is pre-loaded into the chip to effectively prevent aerosol contamination. This approach allows for the simultaneous detection of 21 common respiratory pathogens, with a detection limit of 10 copies per reaction. Furthermore, applications involving clinical samples demonstrate significant practicality. Compared to many traditional in-tube pathogen detection methods and molecular biology technologies that utilize microfluidic chips, this detection chip not only enables simultaneous detection of multiple pathogens but also demonstrates high sensitivity.

关键词： 96-Channel microfuild chip Multiplex pathogen detection Magnetic bead method Respiratory pathogens

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Atomically Precise Nanometer-Sized Pt Catalysts with an Additional Photothermy Functionality

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Angewandte Chemie 2024年第25期136卷

作者： Runguo Wang Dr. Dong Chen Dr. Liang Fang Dr. Wentao Fan Dr. Qing You Guoqing Bian Yue Zhou Dr. Wanmiao Gu Dr. Chengming Wang Dr. Licheng Bai Prof. Dr. Jin Li Prof. Dr. Haiteng Deng Dr. Lingwen Liao Prof. Dr. Jun Yang Prof. Dr. Zhikun Wu Key Laboratory of Materials Physics Anhui Key Laboratory of Nanomaterials and Nanotechnology CAS Center for Excellence in Nanoscience Institute of Solid State Physics HFIPS Chinese Academy of Sciences Hefei 230031 China Key Laboratory of Precision and Intelligent Chemistry University of Science and Technology of China Hefei 230026 P. R. China Institute of Physical Science and Information Technology Anhui University Hefei 230601 P.R. China State Key Laboratory of Multiphase Complex Systems Institute of Process Engineering Chinese Academy of Sciences Beijing 100190 P. R. China Instruments' Center for Physical Science University of Science and Technology of China Hefei 230026 P. R. China Shenzhen Engineering Center for the Fabrication of Two-Dimensional Atomic Crystals Shenzhen Institutes of Advanced Technology Chinese Academy of Sciences Shenzhen 518055 P. R. China Tsinghua University-Peking University Joint Center for Life Sciences School of Life Sciences Tsinghua University Beijing 100084 P.R.China MOE Key Laboratory of Bioinformatics School of Life Sciences Tsinghua University Beijing 100084 P.R.China

Atomically precise ~1-nm Pt nanoparticles (nanoclusters, NCs) with ambient stability are important in fundamental research and exhibit diverse practical applications (catalysis, biomedicine, etc.). However, synthesizing such materials is challenging. Herein, by employing the mixture ligand protecting strategy, we successfully synthesized the largest organic-ligand-protected (~1-nm) Pt 23 NCs precisely characterized with mass spectrometry and single-crystal X-ray diffraction analyses. Interestingly, natural population analysis and Bader charge calculation indicate an alternate, varying charge -layer distribution in the sandwich-like Pt 23 NC kernel. Pt 23 NCs can catalyze the oxygen reduction reaction under acidic conditions without requiring calcination and other treatments, and the resulting specific and mass activities without further treatment are sevenfold and eightfold higher than those observed for commercial Pt/C catalysts, respectively. Density functional theory and d -band center calculations interpret the high activity. Furthermore, Pt 23 NCs exhibit a photothermal conversion efficiency of 68.4 % under 532-nm laser irradiation and can be used at least for six cycles, thus demonstrating great potential for practical applications.

关键词： Pt nanocluster atomically precise ambient stability ORR photothermy

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Allele-specific genome editing of imprinting genes by preferentially targeting non-methylated loci using Staphylococcus aureus Cas9(SaCas9)

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science Bulletin 2019年第21期64卷 1592-1600页

作者： Yajing Liu Jianan Li Changyang Zhou Bin Meng Yu Wei Guang Yang Zongyang Lu Qingmei Shen Yu Zhang Hui Yang Yunbo Qiao School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China Institute of Biochemistry and Cell Biology Chinese Academy of SciencesShanghai 200031China University of Chinese Academy of Sciences Beijing 100049China Institute of Neuroscience State Key Laboratory of NeuroscienceKey Laboratory of Primate NeurobiologyCAS Center for Excellence in Brain Science and Intelligence TechnologyShanghai Institutes for Biological SciencesChinese Academy of SciencesShanghai 200031China Shanghai Institute for Advanced Immunochemical Studies ShanghaiTech UniversityShanghai 200031China Precise Genome Engineering Center School of Life SciencesCuangzhou UniversityGuangzhou 510006China

Allele-specific DNA methylation is the most important imprinting marker localized to differentially methylated regions(DMRs),and aberrant genomic imprinted DNA methylation is associated with some human diseases,including Prader-Willi syndrome and ***,the development of an effective strategy for the precise editing of allele-specific methylated genes is essential for the functional clarification of imprinting elements and the correction of imprinting disorders in human *** discover a feasible allele-specific genome editing tool based on the CRISPR/Cas system,which is an efficient genetargeting technique in various organisms,we examined the targeting efficiency of Staphylococcus aureus Cas9(SaCas9)and Streptococcus pyogenes Cas9(SpCas9)in response to DNA methylation *** found that the targeting efficiency of SaCas9,but not SpCas9,was enhanced by targeted DNA demethylation using the d Cas9-Tet1 catalytic domain(CD)but suppressed by targeted DNA methylation using Dnmt3l-Dnmt3a-d *** in vitro cleavage assay further demonstrated that SaCas9 nuclease activity was inhibited by 5-methylcytosine(5mC)in a synthesized Cp G-containing *** analysis with Ch IP-Q-PCR demonstrated that the non-methylated sequence targeting of Sa Cas9 depends on the binding preference of SaCas9 to non-methylated *** advantage of this feature of SaCas9,we have successfully obtained non-methylated allele-biased targeted embryos/mice for two imprinting genes,H19 and Snrpn,with relatively high efficiencies of 28.6%and 47.4%,*** results indicate that the targeting efficiency of SaCas9 was strongly reduced by DNA *** using SaCas9,we successfully achieved allele-specific genome editing of imprinting genes by preferentially targeting non-methylated loci.

关键词： Allele-specific DNA methylation SaCas9 Imprinting gene

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Highly oxygenated acorane sesquiterpenes from the marine-sourced Trichoderma harzianum CS-152

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Fitoterapia 2025年 106672页

作者： Yan-He Li Xiao-Ming Li Xin Li Xiao-Shan Shi Sui-Qun Yang Hong-Lei Li Bin-Gui Wang CAS and Shandong Province Key Laboratory of Experimental Marine Biology Institute of Oceanology Chinese Academy of Sciences Nanhai Road 7 Qingdao 266071 China Laboratory for Marine Biology and Biotechnology Qingdao Marine Science and Technology Center Wenhai Road 1 Qingdao 266237 China University of Chinese Academy of Sciences Yuquan Road 19A Beijing 100049 China

Three new highly oxygenated acorane sesquiterpenes, namely, 9,10-epoxidicacoren-2,7,8-triol ( 1 ), 8 S -acoren-2,7,8-triol ( 2 ), and 8 R -acoren-2,7,8-triol ( 3 ), along with four known analogs ( 4 – 7) , were isolated from the deep-sea derived fungus Trichoderma harzianum CS-152. Except for compound 7 , the other compounds lack conjugation and do not exhibit fluorescence under UV light at 254 nm, which posed a challenge in their detection and required careful monitoring during separation. The isolation and purification of these compounds was achieved using traditional silica gel column chromatography. Their chemical structures and relative configurations were elucidated by comprehensive analysis of 1D/2D NMR, HRESIMS, and their absolute configurations were determined by modified Mosher's method. Compounds 1 and 4 displayed inhibitory activities against the aquatic pathogen Aeromonas hydrophila , each with a MIC value 4 μg/mL, while compound 7 showed antibacterial activity against Pseudomonas aeruginosa with a MIC value 4 μg/mL.

关键词： Acorane sesquiterpenes Antibacterial activity Cold-seep Deep sea-derived fungus Trichoderma harzianum

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Enhanced exchange bias in all-oxide heterostructures with cation-ordered ferrimagnetic double-perovskite

npj Spintronics

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npj Spintronics 2024年第1期2卷 1-6页

作者： Xiaofu Qiu Hetian Chen Yuhan Liang Xiaoyu Jiang Jing Ma Di Yi Zelin Wang Tianxiang Nan Yujun Zhang Fangyuan Zhu Zhen Chen State Key Laboratory of New Ceramics and Fine Processing School of Materials Science and Engineering Tsinghua University Beijing 100084 China School of Integrated Circuits Beijing National Research Center for Information Science and Technology Tsinghua University Beijing 100084 China National Center for Electron Microscopy in Beijing School of Materials Science and Engineering Tsinghua University Beijing 100084 China Institute of High Energy Physics Chinese Academy of Sciences Beijing 100049 China Shanghai Synchrotron Radiation Facility Shanghai Advanced Research Institute Chinese Academy of Sciences Shanghai 201204 China Beijing National Laboratory for Condensed Matter Physics Institute of Physics Chinese Academy of Sciences Beijing 100190 China School of Physical Sciences University of Chinese Academy of Sciences Beijing 100049 China

The realization and control of exchange bias (EB) are highly desirable for spintronic applications. All-oxide heterostructures comprised of ferromagnetic and antiferromagnetic/multiferroic oxides provide an ideal platform to enable the electric-field control of EB, promising for energy-efficient memory and logic devices. However, the low block temperature (TB) and small bias field (HEB) hinder further advances towards room-temperature applications. Here, we report an alternative approach to enhance the interface-induced EB by using ferrimagnetic double-perovskite with B-site cation ordering. In heterostructures comprised of double-perovskite Sr2FeReO6(SFRO) and LaFeO3(LFO), a high TB(about 250 K) and large HEBare observed, which is significantly larger than the counterparts with LFO and ferromagnetic oxides. Further analysis suggests that the cation-ordering and ferrimagnetic spin structure of the double-perovskite could contribute significantly to the enhanced exchanged bias when interfacing with G-type antiferromagnets. Our results open a new avenue for developing all-oxides heterostructures for future magnetic technologies.

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Echocardiographic Measurements in Normal Chinese Adults(EMINCA)II focusing on left ventricular and left atrial size and function by three-dimensional echocardiography

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Frontiers of Medicine 2024年第4期18卷 649-663页

作者： Yingbin Wang Yu Zhang Guihua Yao Hong Tang Lixin Chen Lixue Yin Tiangang Zhu Jianjun Yuan Wei Han Jun Yang Xianhong Shu Ya Yang Yulin Wei Yanli Guo Weidong Ren Dongmei Gao Guilin Lu Ji Wu Hongning Yin Yuming Mu Jiawei Tian Lijun Yuan Xiaojing Ma Hongyan Dai Yunchuan Ding Mingyan Ding Qing Zhou Hao Wang Di Xu Mei Zhang Yun Zhang State Key Laboratory for Innovation and Transformation of Luobing Theory Key Laboratory of Cardiovascular Remodeling and Function ResearchChinese Ministry of EducationChinese National Health Commission and Chinese Academy of Medical SciencesDepartment of CardiologyQilu Hospital of Shandong UniversityJinan250012China Department of Cardiology Qilu Hospital of Shandong University(Qingdao)Qingdao266000China Department of Ultrasonography West China HospitalSichuan UniversityChengdu610041China Department of Ultrasonography Shenzhen People’s Hospital/The Second Clinical Medical College of Jinan UniversityShenzhen518020China Department of Ultrasonography Electronic Science and Technology University of ChinaThe Affiliated Sichuan Provincial People’s HospitalChengdu610072China Department of Cardiology Peking University People’s HospitalBeijing100044China Department of Ultrasonography Henan Provincial People’s HospitalZhengzhou463599China Department of Cardiology The First Affiliated Hospital of Harbin Medical UniversityHarbin150007China Department of Echocardiology The First Affiliated Hospital of China Medical UniversityShenyang110001China Department of Echocardiology Zhongshan HospitalFudan UniversityShanghai200032China Department of Echocardiology Beijing Anzhen HospitalCapital Medical UniversityBeijing100029China Department of Cardiology Sun Yat-sen Memorial HospitalSun Yat-sen UniversityGuangzhou510120China Department of Ultrasonography The Southwest Hospital of AMUChongqing400038China Department of Ultrasonography Shengjing Hospital of China Medical UniversityShenyang110136China Department of Ultrasonography China-Japan Union hospital of Jilin UniversityChangchun130033China Department of Ultrasonography First Affiliated HospitalSchool of MedicineShihezi UniversityShihezi832000China Department of Ultrasonography The First Affiliated Hospital of Guangxi Medical UniversityNanning537406China Department of Echocardiology The Second Hospital of Hebei Medical Uni

Current guidelines encourage large studies in a diverse population to establish normal reference ranges for three-dimensional(3D)echocardiography for different ethnic *** study was designed to establish the normal values of 3D-left ventricular(LV)and left atrial(LA)volume and function in a nationwide,population-based cohort of healthy Han Chinese adults.A total of 1117 healthy volunteers aged 18–89 years were enrolled from 28 collaborating laboratories in *** sets of 3D echocardiographic instruments were used,and full-volume echocardiographic images were recorded and transmitted to a core laboratory for image analysis with a vendor-independent off-line ***,866 volunteers(mean age of 48.4 years,402 men)were qualified for final *** parameters exhibited substantial differences between different sex and age groups,even after indexation by body surface *** normal ranges of 3D-LV and 3D-LA volume and function differed from those recommended by the American Society of Echocardiography and the European Association of Cardiovascular Imaging guidelines,presented by the World Alliance Societies of Echocardiography(WASE)study,and from the 2D values in the EMINCA *** normal reference values of 3D echocardiography-derived LV and LA volume and function were established for the first time in healthy Han Chinese *** ranges of 3D-LV and 3D-LA echocardiographic measurements stratified with sex,age,and race should be recommended for clinical applications.

关键词： three-dimensional echocardiography left ventricle left atrium normal reference values Chinese adults

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A comprehensive survey of oracle character recognition: Challenges, datasets, methodology, and beyond

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Pattern Recognition 2026年 169卷

作者： Jing Li Xueke Chi Qiufeng Wang Kaizhu Huang Da-Han Wang Yongge Liu Cheng-Lin Liu College of Science & Technology Ningbo University Ningbo China School of Advanced Technology Xi’an Jiaotong-Liverpool University Suzhou China Duke Kunshan University Suzhou China Fujian Key Laboratory of Pattern Recognition and Image Understanding School of Computer and Information Engineering Xiamen University of Technology Xiamen China Anyang Normal University Anyang China State Key Laboratory of Multimodal Artificial Intelligence Systems Institute of Automation Chinese Academy of Sciences Beijing China

Oracle character recognition — an analysis of ancient Chinese inscriptions found on oracle bones — has become a pivotal field intersecting archaeology, paleography, and historical cultural studies. Traditional methods of oracle character recognition have relied heavily on manual interpretation by experts, which is not only labor-intensive but also limits broader accessibility to the general public. With recent breakthroughs in pattern recognition and deep learning, there is a growing movement toward the automation of oracle character recognition (OrCR), showing considerable promise in tackling the challenges inherent to these ancient scripts. However, a comprehensive understanding of OrCR still remains elusive. Therefore, this paper presents a systematic and structured survey of the current landscape of OrCR research. We commence by identifying and analyzing the key challenges of OrCR. Then, we provide an overview of the primary benchmark datasets and digital resources available for OrCR. A review of contemporary research methodologies follows, in which their respective efficacies, limitations, and applicability to the complex nature of oracle characters are critically highlighted and examined. Additionally, our review extends to ancillary tasks associated with OrCR across diverse disciplines, providing a broad-spectrum analysis of its applications. We conclude with a forward-looking perspective, proposing potential avenues for future investigations that could yield significant advancements in the field.

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Simultaneous optical g, r, i monitoring and IDV periodic analysis for quasar 3C 454.3

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Research in Astronomy and Astrophysics 2019年第10期19卷 43-50页

作者： Jun-Hui Fan Yu-Hai Yuan Hong Wu Feng Wang Jun Tao Min-Feng Gu Center for Astrophysics Guangzhou University Guangzhou 510006 China Astronomy Science and Technology Research Laboratory of Department of Education of Guangdong Province Guangzhou 510006 China Key Laboratory of Optical Astronomy National Astronomical Observatories Chinese Academy of Sciences Beijing 100101 China Shanghai Astronomical Observatory Chinese Academy of Sciences Shanghai 200030 China Key Laboratory for Research in Galaxies and Cosmology Shanghai Astronomical Observatory Chinese Academy of Sciences Shanghai 200030 China

With the 1.26 m National Astronomical Observatory-Guangzhou University Infrared/Optical Telescope(NAGIOT) at Xinglong Station of National Astronomical Observatories, Chinese academy of sciences, we obtained 419 groups of simultaneous observations at g, r and i bands, for the first time,targeting quasar 3 C 454.3 during 15 nights from 2016 October 23 to 2016 December 15. Based on our observations, we investigate the optical variabilities, the relation between brightness and color index, and the periodicity variability. The presented analyses demonstrate that: 1. The maximum variations at the g, r and i bands are △mg|max= 1.015 ± 0.042 mag,△mr|max= 1.188 ± 0.050 mag and △mi|max= 1.305 ± 0.057 mag respectively. 2. During our 15 night monitoring program, intra-day variability was detected on one night(Nov. 2). Also, the brightness increased by A = 15.86% over 50.8 min,then decreased by A = 22.42% over 40.1 min. After a small bright state, its brightness increased again by18.1% over 55 min at the g band. Similar phenomena happened at r and i bands. The intra-day variabilities at the three bands on 2016 November 2 indicate a period of 105 min, which implies a black hole mass of MBH =(0.3 ~ 1.85)× 10^9 M⊙. 3. There is an anti-correlation between color index and magnitude,suggesting the source becomes redder when it brightens.

关键词： galaxies quasars individual (3C 454.3)

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Notice of Violation of IEEE Publication Principles: Tone Mapping Beyond the Classical Receptive Field

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IEEE Transactions on Image Processing 2020年 29卷 4174-4187页

作者： Shao-Bing Gao Min-Jie Tan Zhen He Yong-Jie Li College of Computer Science Sichuan University Chengdu China School of Aeronautics and Astronautics Sichuan University Chengdu China Academy of Military Medical Sciences Beijing China MOE Key Laboratory for NeuroInformation School of Life Science and Technology University of Electronic Science and Technology of China Chengdu China

Notice of Violation of IEEE Publication Principles "Tone Mapping Beyond the Classical Receptive Field," by Shao-Bing Gao, Min-Jie Tan, Zhen He, and Yong-Jie Li in the IEEE Transactions on Image Processing, vol. 29, February 2020, pp. 4174???4187 After careful and considered review of the content of this paper by a duly constituted expert committee, this paper has been found to be in violation of IEEE's Publication Principles. This paper contains significant portions of content from the paper cited below. The original content was copied without attribution (including appropriate references to the original author(s) and/or paper title) and without permission. Due to the nature of this violation, reasonable effort should be made to remove all past references to this paper, and future references should be made to the following paper "Colour Constancy Beyond the Classical Receptive Field," by Arash Akbarinia, and C. Alejandro Parraga, in the IEEE Transactions on Pattern analysis and Machine Intelligence, vol. 40, no. 9,September 2018, pp. 2081???2094 Some neurons in the primary visual cortex (V1) of human visual system (HVS) conduct dynamic center-surround computation, which is thought to contribute to compress the high dynamic range (HDR) scene and preserve the details. We simulate this dynamic receptive field (RF) property of V1 neurons to solve the so-called tone mapping (TM) task in this paper. The novelties of our method are as follows. (1) Cortical processing mechanisms of HVS are modeled to build a local TM operation based on two Gaussian functions whose kernels and weights adapt according to the center-surround contrast, thus reducing halo artifacts and effectively enhancing the local details of bright and dark parts of image. (2) Our method uses an adaptive filter that follows the contrast levels of the image, which is computationally very efficient. (3) The local fusion between the center and surround responses returned by a cortical processing flow and the glo

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Phase coexistence and evolution in sol-gel derived BY-PT-PZ ceramics with significantly enhanced piezoelectricity and high temperature stability

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Journal of Materiomics 2019年第3期5卷 394-403页

作者： Kai Cai Xue Yan Pingye Deng Li Jin Yang Bai Fei Zeng Dong Guo Beijing Center for Physical and Chemical Analysis Beijing100089China School of Materials Science and Engineering Beihang UniversityBeijing100191China Electronic Materials Research Laboratory School of Electronic and Information EngineeringXi'an Jiaotong UniversityXi'an710049China School of Materials Science and Engineering University of Science and Technology BeijngBeijing100083China School of Material Science and Engineering Tsinghua UniversityBeijing100084China Institute of Acoustics Chinese Academy of SciencesBeijing100190China

In this study,high Curie Temperature(T_(c))perovskite ceramics of optimized composition 0.55(0.1BiYbO_(3)-0.9PbTiO_(3))-0.45PbZrO_(3) with unique double orthorhombic main phases were prepared by a modified solgel *** to the usual solid-state prepared sample,the sol-gel derived sample has a 1.6 times higher d_(33) of 325 pC/N,a 2.4 times higher remnant polarization,and a much better high temperature stability with similar depolarization temperature(T_(d))and T_(c).Comprehensive analysis of the xerogel prepared over a wide calcination temperature(T_(cal))range of 300-1000℃ revealed that perovskite structure appeared at only 400℃ and it became the main phase above 500℃.Comparison of XRD refinement results showed that calcination and sintering induced subtle and continuous phase transition,namely,the 400-900℃ calcined powders with coexisted tetragonal(P4mm)and orthorhombic(Pbam)phase changed to a rather stable double orthorhombic(Pmmm and Pbam)main phase in all the differently sintered ceramics,as similar to the 1000C calcined *** stable phase coexistence well explains the enhanced *** results also demonstrate that optimized sol-gel processing can provide high T_(c) ceramics with desirable multi-phase structure and significantly enhanced performance at a lower temperature.

关键词： Piezoelectricity Curie temperature Phase transition Sol-gel Perovskite

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