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Large-scale Identification and Time-course Quantification of Ubiquitylation Events During Maize Seedling De-etiolation

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Genomics, Proteomics & Bioinformatics 2019年第6期17卷 603-622页

作者： Yue-Feng Wang Qing Chao Zhe Li Tian-Cong Lu Hai-Yan Zheng Cai-Feng Zhao Zhuo Shen Xiao-Hui Li Bai-Chen Wang Photosynthesis Research Center CAS Key Laboratory of PhotobiologyInstitute of BotanyChinese Academy of SciencesBeijing 100093China Crop Germplasm Resources Institute Jilin Academy of Agricultural SciencesGongzhuling 136100China Precision Scientific(Beijing)Co. Beijing 100085China Advanced Biotechnology and Application Research Center School of Chemistry and Biological EngineeringUniversity of Science and Technology BeijingBeijing 100083China Center for Advanced Biotechnology and Medicine Biological Mass Spectrometry FacilityRutgers UniversityPiscatawayNJ 08855USA College of Life Sciences Graduate University of Chinese Academy of SciencesBeijing 100049China

The ubiquitin system is crucial for the development and fitness of higher ***-etiolation, during which green plants initiate photomorphogenesis and establish autotrophy, is a dramatic and complicated process that is tightly regulated by a massive number of ubiquitylation/de-ubiquitylation events. Here we present site-specific quantitative proteomic data for the ubiquitylomes of de-etiolating seedling leaves of Zea mays L.(exposed to light for 1, 6, or 12 h)achieved through immunoprecipitation-based high-resolution mass spectrometry(MS). Through the integrated analysis of multiple ubiquitylomes, we identified and quantified 1926 unique ubiquitylation sites corresponding to 1053 proteins. We analyzed these sites and found five potential ubiquitylation motifs, KA, AXK, KXG, AK, and TK. Time-course studies revealed that the ubiquitylation levels of 214 sites corresponding to 173 proteins were highly correlated across two replicate MS experiments, and significant alterations in the ubiquitylation levels of 78 sites(fold change >1.5) were detected after de-etiolation for 12 h. The majority of the ubiquitylated sites we identified corresponded to substrates involved in protein and DNA metabolism, such as ribosomes and ***, multiple ubiquitylation sites were detected in proteins whose functions reflect the major physiological changes that occur during plant de-etiolation, such as hormone synthesis/signaling proteins, key C4 photosynthetic enzymes, and light signaling proteins. This study on the ubiquitylome of the maize seedling leaf is the first attempt ever to study the ubiquitylome of a C4 plant and provides the proteomic basis for elucidating the role of ubiquitylation during plant de-etiolation.

关键词： Maize seedling leaf De-etiolation Ubiquitylation C4 photosynthetic enzymes Ribosome

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Pyridinic nitrogen-modulated carbon nanotubes facilitate dual-electron transfer for polymerization-enhanced pollutant removal

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Applied Catalysis B: Environment and Energy 2025年 378卷

作者： Yi Shen Qichao Shen Chao Zhu Yajie Lin Qile Fang Renlan Liu Jun Wang Lun Lu Shuang Song Department of Environment Zhejiang University of Technology Hangzhou 310014 China State Key Laboratory of Green Chemical Synthesis and Conversion Zhejiang University of Technology Hangzhou 310014 China Shaoxing Research Institute Zhejiang University of Technology Shaoxing 312000 China China general consulting & investment co. ltd. Beijing 100073 China Advanced Institute of Natural Sciences Beijing Normal University at Zhuhai Zhuhai 519087 China College of Life and Environmental Science Wenzhou University Wenzhou 325035 China School of Environmental Science and Engineering State Key Laboratory of Separation Membranes and Membrane Processes Tiangong University Tianjin 300387 China Guangzhou Institute of Energy Conversion Chinese Academy of Sciences (CAS) Guangzhou 510640 China

This study explores the regulation of polymerization-driven pollutant degradation through electronic structure modulation of carbon-based catalysts. Nitrogen-doped carbon nanotubes (N-CNTs) with tunable pyridinic N content were synthesized by varying N precursors and applied in peroxydisulfate (PDS)-activated systems to evaluate their direct oxidative transfer process (DOTP) pathway performance. The N-CNT with the highest pyridinic N content (65.1 %) achieved nearly 100 % phenol (PhOH) removal with a rate constant (K obs ) of 0.23 min −1 , 3.8 times higher than pristine CNTs, along with 90.0 % DOTP contribution and a 250 % increase in PDS utilization. Mechanistic analysis revealed that pyridinic N induced polarized electron density, enhanced dipole-driven pollutant adsorption, and significantly lowered the activation energy of the dual-electron transfer DOTP pathway. This work establishes an effective strategy for enhancing polymerization-based degradation via electronic modulation, offering new theoretical and technological insights for the development of efficient and low-carbon water treatment catalysts.

关键词： Carbon nanotube Dual-electron transfer Peroxydisulfate activation Polymerization pathway Pyridine-N doping

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Case study of aeroengine parameter prediction based on MIV and ELM

Case study of aeroengine parameter prediction based on MIV a...

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2019 International Conference on Sensing, Diagnostics, Prognostics, and Control, SDPC 2019

作者： Li, Yingshun Wang, Fuyang Sun, Ximing Yi, Xiaojian Dalian University of Technology Key Laboratory of Intelligent Control and Optimization for Industrial Equipment Ministry of Education !!!Dalian University of Technology @@@School of Control Science and Engineering Dalian China Chinese Academy of Sciences School of Mechatronical Engineering Beijing China

ISBN: (纸本)9781728101996

Aiming at the problems existing in the current prediction methods of aeroengine parameters, such as the difficulty in parameter selection, the slow training speed and the tendency to fall into local optimal solution of traditional BP neural network algorithm, this paper proposes the prediction method of aeroengine performance parameters based on mean influence value (MIV) algorithm and extreme learning machine (ELM). Firstly, we preprocess the sample data. Secondly, screening out the main parameters that affect the predicted parameters by MIV algorithm, attribute reduction is realized, the result of attribute reduction is taken as the input to train an ELM. Finally, using the test samples to do the test. The testing results show that the algorithm is faster and more accurate in parameter prediction. © 2019 IEEE.

关键词： Forecasting

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In-Situ Integration of a Hydrophobic and Fast-Zn2+-Conductive Inorganic Interphase to Stabilize Zn Metal Anodes

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Angewandte Chemie 2023年第27期135卷

作者： Mengyu Liu Wentao Yuan Dr. Guoqiang Ma Kaiyue Qiu Dr. Xueyu Nie Prof. Yongchang Liu Prof. Shigang Shen Prof. Ning Zhang College of Chemistry and Materials Science Key Laboratory of Analytical Science and Technology of Hebei Province Hebei University Baoding 071002 P. R. China Contribution: Conceptualization (equal) Data curation (lead) Formal analysis (lead) Investigation (lead) Methodology (equal) Writing - original draft (equal) Contribution: Data curation (equal) Formal analysis (equal) Investigation (equal) Methodology (equal) Software (lead) Validation (equal) Contribution: Formal analysis (equal) Validation (equal) Contribution: Formal analysis (equal) Beijing Advanced Innovation Center for Materials Genome Engineering Institute for Advanced Materials and Technology State Key Laboratory for Advanced Metals and Materials University of Science and Technology Beijing Beijing 100083 P. R. China Contribution: Conceptualization (equal) Formal analysis (equal) Funding acquisition (supporting) Resources (equal) Supervision (equal) Writing - review & editing (equal) Contribution: Formal analysis (equal) Resources (equal) Supervision (supporting) Contribution: Conceptualization (lead) Formal analysis (equal) Funding acquisition (lead) Investigation (equal) Methodology (equal) Resources (lead) Supervision (lead) Writing - original draft (lead) Writing - review & editing (lead)

The irreversible issues of Zn anode stemming from dendrite growth and water-induced erosion have severely hindered the commercialization of rechargeable aqueous Zn batteries. Herein, a hydrophobic and fast-Zn 2+ -conductive zinc hexacyanoferrate (HB-ZnHCF) interphase layer is in situ integrated on Zn by a rapid room-temperature wet-chemistry method to address these dilemmas. Different from currently proposed hydrophilic inorganic cases, the hydrophobic and compact HB-ZnHCF interphase effectively prevents the access of water molecules to Zn surface, thus avoiding H 2 evolution and Zn corrosion. Moreover, the HB-ZnHCF with large internal ion channels, strong zincophilicity, and high Zn 2+ transference number (0.86) permits fast Zn 2+ transport and enables smooth Zn deposition. Remarkably, the resultant HB-ZnHCF@Zn electrode delivers unprecedented reversibility with 99.88 % Coulombic efficiency over 3000 cycles, realizes long-term cycling over 5800 h (>8 months, 1 mA cm −2 ) and 1000 h (10 mA cm −2 ), and assures the stable operation of full Zn battery with both coin- and pouch-type configurations.

关键词： Aqueous Zn Battery Hydrophobic Interphase Inorganic Interphase Zinc Hexacyanoferrate Zn Metal Anode

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Observational study of intermittent solar jets: p-mode modulation

arXiv

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arXiv 2023年

作者： Cai, Qiuzhuo Ruan, Guiping Zheng, Chenxi Schmieder, Brigitte Guo, Jinhan Chen, Yao Su, Jiangtao Liu, Yang Liu, Jihong Cao, Wenda Shandong Provincial Key Laboratory of Optical Astronomy and Solar-Terrestrial Environment Institute of Space Sciences Shandong University Weihai264209 China Centre for Mathematical Plasma-Astrophysics Department of Mathematics KU Leuven Celestijnenlaan 200B Leuven3001 Belgium LESIA Observatoire de Paris Université PSL CNRS Sorbonne Université Université de Paris 5 place Jules Janssen Meudon92190 France School of Astronomy and Space Science Key Laboratory for Modern Astronomy and Astrophysics Nanjing University Nanjing210023 China National Astronomical Observatories Chinese Academy of Sciences Beijing100101 China School of Astronomy and Space Sciences University of Chinese Academy of Sciences Beijing100049 China Shi Jiazhuang University Shi Jiazhuang050035 China Center for Solar-Terrestrial Research New Jersey Institute of Technology 323 Martin Luther King Blvd. NewarkNJ07102 United States Big Bear Solar Observatory 40386 North Shore Lane Big Bear CityCA92316 United States

Aims. Recurring jets are observed in the solar atmosphere. They can erupt intermittently over a long period of time. By the observation of intermittent jets, we wish to understand what causes the characteristics of the periodic eruptions. Methods. We report intermittent jets observed by the Goode Solar Telescope (GST) with the TiO Broadband Filter Imager (BFI), the Visible Imaging Spectrometer (VIS) in Hα, and the Near-InfraRed Imaging Spectropolarimeter (NIRIS). The analysis was aided and complemented by 1400 Å and 2796 Å data from the Interface Region Imaging Spectrograph (IRIS). These observational instruments allowed us to analyze the temporal characteristics of the jet events. By constructing the Hα Dopplergrams, we found that the plasma first moves upward, but during the second phase of the jet, the plasma flows back. Working with time slice diagrams, we investigated the characteristics of the jet dynamics. Results. The jet continued for up to 4 hours. The time-distance diagram shows that the peak of the jet has clear periodic-eruption characteristics (5 minutes) during 18:00 UT-18:50 UT. We also found a periodic brightening phenomenon (5 minutes) during the jet bursts in the observed bands in the transition region (1400 Å and 2796 Å), which may be a response to intermittent jets in the upper solar atmosphere. The time lag is 3 minutes. Evolutionary images in the TiO band revealed a horizontal movement of the granulation at the location of the jet. By comparison to the quiet region of the Sun, we found that the footpoint of the jet is enhanced at the center of the Hα spectral line profile, without significant changes in the line wings. This suggests prolonged heating at the footpoint of the jet. In the mixed-polarity magnetic field region of the jet, we observed the emergence of magnetic flux, its cancellation, and shear, indicating possible intermittent magnetic reconnection. This is confirmed by the nonlinear force-free field model, which was reconstructed u

关键词： Infrared devices

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Long-term biotic responses and anthropogenic impacts in dryland lake ecosystems of northwestern China over the past two centuries

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Palaeogeography, Palaeoclimatology, Palaeoecology 2025年 675卷

作者： Lixiong Xiang Dongliang Zhang Wenjia Wang Muhammad Farqan Xueqing Song Chuangzi Yan Yuan Li Xuemei Chen Mingrui Qiang Guangjie Chen Ke Zhang Jianbao Liu Xiaohua Gou Erik Jeppesen Xiaozhong Huang Key Laboratory of Western China's Environmental Systems (Ministry of Education) College of Earth and Environmental Sciences Lanzhou University Lanzhou 730000 China National Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands Xinjiang Institute of Ecology and Geography Chinese Academy of Sciences 818 Beijing South Road Urumqi 830011 China Key Laboratory of Desert and Desertification Northwest Institute of Eco-Environment and Resources Chinese Academy of Sciences Lanzhou 730000 China University of Chinese Academy of Sciences Beijing 100049 China School of Geography South China Normal University Guangzhou China Yunnan Key Laboratory of Plateau Geographical Processes and Environmental Changes Faculty of Geography Yunnan Normal University Kunming 650500 China Key Laboratory of Lake and Watershed Science for Water Security Nanjing Institute of Geography and Limnology Chinese Academy of Sciences Nanjing 210008 China College of Urban and Environmental Sciences Peking University Beijing 100871 China State Key Laboratory of Tibetan Plateau Earth System Resources and Environment (TPESRE) Institute of Tibetan Plateau Research Chinese Academy of Sciences Beijing 100101 China Department of Ecoscience and Water Technology Centre/WATEC Aarhus University C.F. Møllers Allé 4-6 Aarhus C 8000 Denmark Sino-Danish Centre for Education and Research (SDC) University of Chinese Academy of Sciences Beijing China Institute for Ecological Research and Pollution Control of Plateau Lakes School of Ecology and Environmental Science Yunnan University Kunming China

In recent decades, lakes in arid central Asia have undergone severe ecological and environmental changes due to accelerated climate warming and intense human activities. However, there have been few studies that integrate multi-community dynamics, long-term historical trends, and drivers of ecological change, limiting our ability to effectively manage the evolution of these freshwater ecosystems. In this paper, we analyze high-resolution biotic proxies (subfossil cladocerans and diatoms) from Bosten Lake and Ailike Lake, northwest China, over the past two centuries, and compare these records with other lakes in the region to reveal changes in lake ecosystems. Our results indicate that natural climate variability was the primary driver of a first transition (∼1910 CE), whereas anthropogenic forcings predominantly drove a second transition (∼1960 CE) in these dryland lakes. After ∼1960 CE, planktonic eutrophic cladoceran species such as Bosmina longirostris and diatom species including Fragilaria cf. crotonensis , Cyclotella atomus , and Cyclotella meneghiniana became more dominant. Redundancy analysis and variance partitioning analysis suggest that changes in community assemblages were mainly driven by nitrogen loading (TN and/or δ 15 N), with additional influences related to lake productivity (Chl-a and TOC) and global warming. Furthermore, another shift in cladoceran composition in Bosten Lake, commencing ∼1990 CE, from large-sized Daphnia to small-sized Bosmina , was likely caused by increased predation pressure as result of the introduction of commercial fish farming. Our findings highlight the importance of understanding the complex interactions of lake ecological shifts with compounded stressors, both climate and anthropogenic forcings, for sustainable water management strategies, enhancing ecological resilience, and protecting freshwater ecosystems from further degradation.

关键词： Anthropogenic effects Cladocera Climate forcing Diatoms Regime shift

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Unraveling bitter peptides in wheat protein hydrolysates

Food Chemistry: Molecular Sciences

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Food Chemistry: Molecular sciences 2025年 10卷

作者： Wenmeng He Shiqin Wang Beibei Wang Mingming Wang Pan Liao Department of Life Science Faculty of Science and Technology Beijing Normal-Hong Kong Baptist University Zhuhai Guangdong China Department of Biology Hong Kong Baptist University Kowloon Tong Hong Kong SAR China School of Life Science and Bioengineering Jining University Jining 273155 China School of Food Science and Technology Ocean University of China 26003 Qingdao China Institute of Systems Medicine and Health Sciences Hong Kong Baptist University Kowloon Tong Hong Kong SAR China State Key Laboratory of Environmental and Biological Analysis Hong Kong Baptist University Hong Kong SAR China State Key Laboratory of Agrobiotechnology The Chinese University of Hong Kong Hong Kong SAR China

Wheat protein hydrolysates (WPHs) hold promise for functional food applications, but papain (EC 3.4.22.2)-mediated hydrolysis often results in undesirable bitterness and low yields of small peptides. To address this, we employed in silico strategies to systematically characterize bitter peptides in papain-derived WPHs and elucidate their molecular interactions with the human bitter taste receptor TAS2R14. Enzymatic hydrolysis of wheat proteins was simulated using the BIOPEP-UWM database, identifying 36 potential bitter peptides. Molecular docking studies revealed four critical binding residues (Asn157, Ile262, Trp89, and Phe247) on TAS2R14 that mediate bitterness perception. Notably, peptides rich in proline (Pro), phenylalanine (Phe), and tryptophan (Trp) exhibited heightened bitterness, while glycine (Gly)- and glutamic acid (Glu)-containing peptides were associated with reduced bitterness. These findings provide a mechanistic basis for mitigating bitterness in WPHs through selective enrichment or exclusion of peptides with specific amino acid profiles. By enabling targeted design of hydrolysis processes, this work advances the application of WPHs as palatable, bioactive ingredients in the food industry.

关键词： TAS2R14 Bitterness Molecular docking In silico ToxinPred ADMET

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Breaking the kinetics-load dilemma in zinc-ion capacitor thick electrodes via structure-interface synergy: Hierarchical nanoarchitectures with dual-site adsorption energetics modulation

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Energy Storage Materials 2025年 79卷

作者： Zhiwei Tian Zixuan Guo Gaigai Duan Yong Huang Weijun Li Xiaoshuai Han Chunmei Zhang Shuijian He Haimei Mao Shaohua Jiang Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources International Innovation Center for Forest Chemicals and Materials College of Materials Science and Engineering Nanjing Forestry University Nanjing 210037 China Institute of Micro/Nano Materials and Devices Ningbo University of Technology Ningbo Zhejiang 315211 China Institute of Materials Science and Devices School of Materials Science and Engineering Suzhou University of Science and Technology Suzhou 215009 China Key Laboratory of Quality Safe Evaluation and Research of Degradable Material State Administration for Market Regulation Hainan Academy of Inspection and Testing Haikou Hainan 570203 China

The thick electrode of zinc ion capacitors (ZICs) is limited by the performance contradiction between fast reaction kinetics and high-quality load. The key to break through this bottleneck is to clarify the multi-physical field coupling mechanism inside the electrode and reveal the synergistic energy storage law of ion cross-scale transport and interface adsorption/desorption. In this study, a “structure-interface” collaborative optimization strategy was proposed: based on the natural wood framework, a three-dimensional interconnected multi-level pore network was constructed by resin to realize the precise matching of pore size and hydrated zinc ions, and N/O diatomic doping was introduced to regulate the surface charge distribution. The WPNK2 electrode exhibits a satisfactory capacity of 5.8 mAh cm –2 and maintains 1.9 mAh cm –2 at 200 mA cm –2 . At the same time, it provides a surprising capacity of 10.9 mAh cm –2 when the load is increased to 82.75 mg cm –2 . Combined with in-situ spectroscopy, DFT, and electrochemical kinetic analysis, the multi-level energy storage mechanism of ion transport and interface adsorption in thick electrodes was revealed. This work provides a full-chain research paradigm of “structural design-interface engineering-mechanism analysis” for solving the “kinetics-load” trade-off problem of thick electrode materials, which will promote the development of high energy density ZICs devices.

关键词： Crystallization kinetics

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Fairness analysis and compensation strategy in the Triangle of Central China driven by water-carbon-ecological footprints

Research Square

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Research Square 2021年

作者： Chen, Yizhong Lu, Hongwei Li, Jing Qiao, Youfeng Yan, Pengdong Ren, Lixia Xia, Jun School of Economics and Management Hebei University of Technology Tianjin300401 China Key Laboratory of Water Cycle and Related Land Surface Processes Institute of Geographic Science and Natural Resources Research Chinese Academy of Sciences Beijing100101 China Hebei Key Laboratory of Environmental Change and Ecological Construction College of Resource and Environment Science Hebei Normal University Shijiazhuang050024 China State Key Laboratory of Hydraulic Engineering Simulation and Safety Tianjin University Tianjin300072 China State Key Laboratory of Water Resources & Hydropower Engineering Sciences Wuhan University Wuhan430000 China Shanxi Institute of Energy Shanxi030600 China

This study proposes water–carbon–ecological footprints to form footprint family indicators for identifying the ecological compensation and regional development equilibrium in the Triangle of Central China (TOCC). The occupation of natural capital stock and flow consumption can be illustrated through a three-dimensional ecological footprint model, and Gini coefficient is integrated into the evaluation framework for fairness measurement from various aspects. Quantificational ecological compensation standards can be given with concerns of ecological resource conversion efficiency and willingness to pay indicators. Results reveal that there exit rising trends in ecological and carbon footprints in the TOCC from 2000 to 2015, while its water footprint presents a fluctuating trend. A majority of average Gini coefficients exceed the warning value (i.e., 0.4) under different footprints, implying a relatively poor overall fairness of regional development. In terms of water footprint, the relatively higher compensation expenses exist in Jingmen, Xiangtan, and Yichun, while Yichang, Zhuzhou, and Fuzhou have higher received compensation values as compared with other cities. When it comes to carbon footprint, Wuhan, Loudi, and Xinyu should pay higher compensation expenses due to their overuse of biological resources. The highest amounts of compensation expense appear in Nanchang and Wuhan from the perspective of ecological footprint. © 2021, CC BY.

关键词： Carbon footprint

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Advanced high-entropy alloys for nanoelectrocatalysts: Characterization, structure design, preparation and applications

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Chemical Engineering Journal 2025年 516卷

作者： Shiyuan Wang Gaigai Duan Xiaoshuai Han Qing Shi Yong Huang Chunmei Zhang Shuijian He Hongliang Zhao Chunxin Ma Shaohua Jiang Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources International Innovation Center for Forest Chemicals and Materials College of Materials Science and Engineering Nanjing Forestry University Nanjing 210037 China Institute of Micro/Nano Materials and Devices Ningbo University of Technology Ningbo 315211 Zhejiang China Institute of Materials Science and Devices School of Materials Science and Engineering Suzhou University of Science and Technology Suzhou 215009 China Key Laboratory of Quality Safe Evaluation and Research of Degradable Material State Administration for Market Regulation Hainan Academy of Inspection and Testing Haikou Hainan 570203 China

High-entropy alloy (HEA) nanomaterials are widely applied in various electrocatalytic fields due to their unique multi-principal element structures and high-entropy effects, which endow them with excellent electrocatalytic properties, including high activity, good stability, and corrosion resistance. In this review, we summarize recent progress in HEA nanomaterials with different dimensions for electrocatalytic applications. The preparation methods and applications of HEA catalysts with various dimensions, as well as their electrocatalytic properties, structures, characterizations, and computational simulations, are discussed in detail. A comprehensive analysis of the performance of HEA nanomaterials with different dimensions in key electrocatalytic reactions, such as the hydrogen evolution reaction (HER), oxygen evolution reaction (OER), and oxygen reduction reaction (ORR) is provided, with comparisons of the effects of different dimensions on electrocatalytic activity. The catalytic mechanisms of HEA nanomaterials with different sizes, including active site formation and reaction pathway regulation, are also thoroughly discussed to better understand their electrocatalytic behavior. Finally, the challenges faced by HEA nanomaterials of various sizes in electrocatalysis are addressed, and future research directions and potential solutions are proposed. Through the comprehensive discussion of these aspects, researchers can gain a deeper understanding of the application of HEA nanomaterials with different sizes in electrocatalysis, thus promoting further research and innovation in this field.

关键词： Electrocatalysis HER High Entropy Alloys Nanomaterials Properties Synthesis

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