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Carbendazim residue in plant-based foods in China:Consecutive surveys from 2011 to 2020

Environmental science and Ecotechnology 2024年第1期17卷 109-118页

作者： Dou Wang Guiling Yang Xiao Yun Ting Luo Hao Guo Liying Pan Wei Du Yanhua Wang Qiang Wang Pu Wang Qinghua Zhang Yun Li Nan Lin State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Laboratory(Hangzhou)for Risk Assessment of Agricultural Products of Ministry of AgricultureInstitute of Agro-product Safety and NutritionZhejiang Academy of Agricultural SciencesHangzhou310021ZhejiangChina College of Urban and Environmental Sciences Peking UniversityBeijing100871China Yunnan Provincial Key Laboratory of Soil Carbon Sequestration and Pollution Control Faculty of Environmental Science&EngineeringKunming University of Science&TechnologyKunming650500China Hubei Key Laboratory of Industrial Fume and Dust Pollution Control School of Environment and HealthJianghan UniversityWuhan430056China State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijing100085China Key Laboratory of Agro-Product Quality and Safety of Ministry of Agriculture Institute of Quality Standards and Testing Technology for Agro-ProductsChinese Academy of Agricultural SciencesBeijing100081China Department of Environmental Health School of Public HealthShanghai Jiao Tong UniversityShanghai200025China

Carbendazim,a widely used fungicide in China,has been found to have reproductive toxicity and mutagenic ***,information on the spatial-temporal variations of carbendazim residues in food in China is ***,we investigated the presence of carbendazim in China's plant-based foods from 2011 to 2020,evaluated the spatial-temporal characteristics,and assessed the associated exposure risks in the Chinese *** results revealed a high detection frequency of carbendazim in fruits(26.4%)and high concentrations in vegetables(~110 mg kg^(-1)),indicating widespread misuse of the *** acute risks of consuming certain vegetables and cereals exceeded the recommended limits by up to 12 and 5 times,*** there has been a decline in carbendazim residue levels in food since the implementation of the Chinese government's action plan for zero growth of pesticide use in 2015,some provinces still exhibited high levels of carbendazim in multiple food categories,which were positively correlated with annual pesticide *** highlight that carbendazim contamination reflects the broader issue of pesticide use in *** emphasizes the need for committed and targeted national policies to reduce carbendazim residues in food and suggests that such measures could also regulate the use of other pesticides,given that pesticide abuse in China is not limited to specific *** call for the re-evaluation of maximum residue limits of carbendazim,particularly in highly consumed foods such as cereals.

关键词： Carbendazim Plant-based foods China Spatial-temporal variations Exposure and risk assessment

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An Adjusted Gray Map Technique for Constructing Large Four-Level Uniform Designs

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Journal of Systems science & Complexity 2023年第1期36卷 433-456页

作者： ELSAWAH A M VISHWAKARMA G K MOHAMED H S FANG Kai-Tai Department of Statistics and Data Science Faculty of Science and TechnologyBeijing Normal University-Hong Kong Baptist University United International CollegeZhuhai 519087China Guangdong Provincial Key Laboratory of Interdisciplinary Research and Application for Data Science BNU-HKBU United International CollegeZhuhai 519087China Department of Mathematics Faculty of ScienceZagazig UniversityZagazig 44519Egypt Department of Mathematics&Computing Indian Institute of Technology DhanbadDhanbad 826004India College of Transportation and Civil Engineering Fujian Agriculture and Forestry UniversityFuzhou 350002China The Key Lab of Random Complex Structures and Data Analysis The Chinese Academy of SciencesBeijing 100190China

A uniform experimental design(UED)is an extremely used powerful and efficient methodology for designing experiments with high-dimensional inputs,limited resources and unknown underlying models.A UED enjoys the following two significant advantages:(i)It is a robust design,since it does not require to specify a model before experimenters conduct their experiments;and(ii)it provides uniformly scatter design points in the experimental domain,thus it gives a good representation of this domain with fewer experimental trials(runs).Many real-life experiments involve hundreds or thousands of active factors and thus large UEDs are *** large UEDs using the existing techniques is an NP-hard problem,an extremely time-consuming heuristic search process and a satisfactory result is not *** paper presents a new effective and easy technique,adjusted Gray map technique(AGMT),for constructing(nearly)UEDs with large numbers of four-level factors and runs by converting designs with s two-level factors and n runs to(nearly)UEDs with 2^(t−1)s four-level factors and 2tn runs for any t≥0 using two simple transformation *** justifications for the uniformity of the resulting four-level designs are given,which provide some necessary and/or sufficient conditions for obtaining(nearly)uniform four-level *** results show that the AGMT is much easier and better than the existing widely used techniques and it can be effectively used to simply generate new recommended large(nearly)UEDs with four-level factors.

关键词： Aberration adjusted Gray map technique Gray map technique Hamming distance moment aberration threshold accepting algorithm uniform design

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Large low-field reversible magnetocaloric effect in K_(3)Gd(PO_(4))_(2) at sub-Kelvin temperature

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Journal of Rare Earths 2024年第6期42卷 1087-1092,I0003页

作者： Haojie Wang Zhaojun Mo Jianjian Gong Heng Tu Guochun Zhang Jun Shen School of Rare Earths University of Science and Technology of ChinaHefei230026China Ganjiang Innovation Academy Chinese Academy of SciencesGanzhou341119China Center for Crystal Research and Development Key Laboratory of Functional Crystals and Laser TechnologyKey Laboratory of CryogenicsTechnical Institute of Physics and ChemistryChinese Academy of SciencesBeijing100190China Department of Energy and PowerEngineering School of Mechanical EngineeringBeijing Institute of TechnologyBeijing100081China

We synthesized a potassium gadolinium phosphate K3Gd(PO_(4))_(2)with monoclinic structure(P2_(1/m))by the high-temperature solid-state reaction *** lattice constants obtained by Rietveld refinements of XRD curve are a=0.7411 nm,b=0.5624 nm,and c=0.9433 *** and magnetocaloric properties of K_(3)Gd(PO_(4))_(2)were investigated in *** magnetic phase transition temperature of K3Gd(PO_(4))2 was determined by ultra-low temperature testing to be 0.58 K.K_(3)Gd(PO_(4))2 exhibits large magnetic entropy changes(-ΔSM).The maximum-ΔSM are 20.2 and 29.4 J/(kg·K)in the magnetic field variation(ΔH)of 1 and 2 T,respectively,which are larger than that of the commercial Gd_(3)Ga_(5)O_(12)(GGG).The maximum adiabatic temperature change(ΔT_(ad))reaches 5.91 K under a magnetic field change(ΔH)of 1 T.K_(3)Gd(PO_(4))_(2)is a potential magnetic refrigeration material for obtaining sub-Kelvin temperatures.

关键词： Ultra-low temperature Magnetocaloric effect Magnetic entropy change Magnetic refrigeration Rare earths

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Mechanistic insights into the orthogonal functionality of an AHL-mediated quorum-sensing circuit in Yersinia pseudotuberculosis

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Synthetic and Systems Biotechnology 2025年第1期10卷 174-184页

作者： Boyu Luo Shanshan Wu Wei Liu Dongdong Zhang Ruicun Liu Tuoyu Liu Zhi Sun Ziqun Wei Mingyu Liu Zhiyuan Shi Niu Huang Yue Teng State Key Laboratory of Pathogen and Biosecurity Academy of Military Medical SciencesBeijing100071China National Institute of Biological Sciences No.7 Science Park RoadZhongguancun Life Science ParkBeijing102206China Laboratory Department in Second Medical Center of PLA General Hospital Beijing100089China Western Medical Branch of PLA General Hospital Beijing100041China Center for Quantitative Biology Peking-Tsinghua Center for Life SciencesAcademy for Advanced Interdisciplinary StudiesPeking UniversityBeijing100871China School of Pharmaceutical Science and Technology Faculty of MedicineTianjin UniversityTianjin300072China Tsinghua Institute of Multidisciplinary Biomedical Research Tsinghua UniversityBeijing102206China

YpsR,a pivotal regulatory protein in the quorum-sensing(QS)of Yersinia pseudotuberculosis(***),is essential for molecular signaling,yet its molecular mechanisms remain poorly ***,this study systemati-cally investigates the interactions between YpsR and acyl-homoserine lactones(AHLs),shedding light on the selective mechanism of YpsR to various AHL *** molecular docking and surface plasmon resonance(SPR)analysis,we confirmed YpsR’s binding affinities,with the strongest observed for 3OC6-HSL,which notably inhibited *** ***,we engineered a whole-cell biosensor based on YpsR-AHL interaction,which exhibited sensitivity to the signal molecule 3OC6-HSL produced by ***,key YpsR resi-dues(S32,Y50,W54,D67)involved in AHL binding were identified and ***,this research elu-cidates the mechanisms of QS signal recognition in ***,providing valuable insights that support the development of diagnostic tools for detecting *** infections.

关键词： Yersinia pseudotuberculosis Quorum sensing Molecular dynamics Genetic circuit Biosensors

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Characterizing strength and location of continental oil shale with drilling process monitoring in Southern Ordos Basin, China

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Journal of Rock Mechanics and Geotechnical Engineering 2025年第6期17卷 3339-3357页

作者： Wu, Siyuan Li, Lihui Li, Xiao Yue, Zhongqi Quentin Department of Civil Engineering The University of Hong Kong Hong Kong Key Laboratory of Shale Gas and Geoengineering Institute of Geology and Geophysics Chinese Academy of Sciences Beijing 100029 China Innovation Academy for Earth Science Chinese Academy of Sciences Beijing 100029 China College of Earth and Planetary Sciences University of Chinese Academy of Sciences Beijing 100049 China School of Science Harbin Institute of Technology Shenzhen 518067 China

The increasing demand for unconventional oil and gas resources, especially oil shale, has highlighted the urgent need to develop rapid and accurate strata characterization methods. This paper is the first case and examines the drilling process monitoring (DPM) method as a digital, accurate, cost-effective method to characterize oil shale reservoirs in the Ordos Basin, China. The digital DPM method provides real-time in situ testing of the relative variation in rock mechanical strength along the drill bit depth. Furthermore, it can give a refined rock quality designation based on the DPM zoning result (RQD(V_DPM)) and a strength-grade characterization at the site. Oil shale has high heterogeneity and low strata strength. The digital results are further compared and verified with manual logging, cored samples, and digital panoramic borehole cameras. The findings highlight the innovative potential of the DPM method in identifying the zones of oil shale reservoir along the drill bit depth. The digital results provide a better understanding of the oil shale in Tongchuan and the potential for future oil shale exploration in other regions. © 2024 Institute of Rock and Soil Mechanics, Chinese academy of sciences

关键词： Constant penetration rate Continental oil shale Digital factual drilling data Drilling process monitoring (DPM) Fracture Time series algorithm

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Isolation and characterization of three pairs of verrucosidin epimers from the marine sediment‑derived fungus Penicillium cyclopium and confguration revision of penicyrone A and related analogues

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Marine Life science & technology 2023年第2期5卷 223-231页

作者： Yan-He Li Attila Mándi Hong-Lei Li Xiao-Ming Li Xin Li Ling-Hong Meng Sui-Qun Yang Xiao-Shan Shi Tibor Kurtán Bin-Gui Wang CAS and Shandong Province Key Laboratory of Experimental Marine Biology Institute of OceanologyChinese Academy of Sciencesand Laboratory of Marine Biology and Biotechnology at the Qingdao National Laboratory for Marine Science and TechnologyQingdao 266071China School of Marine Science University of Chinese Academy of SciencesBeijing 100049China Department of Organic Chemistry University of DebrecenEgyetem Tér 1Debrecen 4032Hungary Center for Ocean Mega-Science Chinese Academy of SciencesQingdao 266071China

Verrucosidins,a methylatedα-pyrone class of polyketides rarely reported upon,have been implicated in one or more neurological *** the signifcance of verrucosidins as neurotoxins,the absolute confgurations of most of the derivatives have not been accurately characterized *** this study,three pairs of C-9 epimeric verrucosidin derivatives,including the known compounds penicyrones A and B(1a/1b)and 9-O-methylpenicyrones A and B(2a/2b),the new compounds 9-O-ethylpenicyrones A and B(3a/3b),together with the related known derivative verrucosidin(4),were isolated and identifed from the culture extract of Penicillium cyclopium SD-413,which was obtained from the marine sediment collected from the East China *** structures were established based on an in-depth analysis of nuclear magnetic resonances(NMR)and mass spectroscopic *** of the absolute confgurations of these compounds was accomplished by Mosher’s method and time-dependent density functional theory(TDDFT)calculations of electronic circular dichroism(ECD)and optical rotation(OR).The confgurational assignment of penicyrone A demonstrated that the previously reported C-6 absolute confguration of verrucosidin derivatives needs to be revised from(6S)to(6R).The 9R/9S epimers of compounds 1–3 were found to exhibit growth inhibition against some pathogenic bacteria,indicating that they have potential as lead compounds for the creation of antimicrobial agents.

关键词： Marine-derived fungus Penicillium cyclopium Verrucosidin derivatives TDDFT-ECD calculations Mosher’s method Antimicrobial activity

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Reveal the mechanism of hepatic oxidative stress in mice induced by photo-oxidation milk using multi-omics analysis techniques

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Journal of Advanced Research 2025年 72卷 53-70页

作者： Tan, Sijia Li, Qiangqiang Guo, Can Chen, Sumeng Kamal-Eldin, Afaf Chen, Gang Key Laboratory of Geriatric Nutrition and Health (Beijing Technology and Business University) Ministry of Education 100048 China Key Laboratory of Agro-Product Quality and Safety Institute of Quality Standards and Testing Technology for Agro-Products Chinese Academy of Agricultural Sciences (CAAS) Beijing 100081 China Institute of Apicultural Research Chinese Academy of Agricultural Sciences (CAAS) Beijing 100093 China China Agricultural University Beijing 100193 China College of Food and Agriculture Department of Food Nutrition and Health (CFA) United Arab Emirates University Al Ain *** United Arab Emirates

Introduction: Photo-oxidation is recognized as a contributor to the deterioration of milk quality, posing potential safety hazards to human health. However, there has been limited investigation into the impact of consuming photo-oxidized milk on health. Objectives: This study employs multi-omics analysis techniques to elucidate the mechanisms by which photo-oxidized milk induces oxidative stress in the liver. Methods: Mouse model was used to determine the effect of the gavage administration of milk with varying degrees of photo-oxidation on the mouse liver. The damage degree was established by measuring serum markers indicative of oxidative stress, and with a subsequent histopathological examination of liver tissues. In addition, comprehensive metabolome, lipidome, and transcriptome analyses were conducted to elucidate the underlying molecular mechanisms of hepatic damage caused by photo-oxidized milk. Results: A significant elevation in the oxidative stress levels and the presence of hepatocellular swelling and inflammation subsequent to the gavage administration of photo-oxidized milk to mice. Significant alterations in the levels of metabolites such as lumichrome, all-trans-retinal, L-valine, phosphatidylglycerol, and phosphatidylcholine within the hepatic tissue of mice. Moreover, photo-oxidized milk exerted a pronounced detrimental impact on the glycerophospholipid metabolism of mice liver. The peroxisome proliferator-activated receptors (PPAR) signaling pathway enrichment appreciated in the animals that consumed photo-oxidized milk further supports the substantial negative influence of photo-oxidized milk on hepatic lipid metabolism. Gene set enrichment and interaction analyses revealed that photo-oxidized milk inhibited the cytochrome P450 pathway in mice, while also affecting other pathways associated with cellular stress response and lipid biosynthesis. Conclusion: This comprehensive study provides significant evidence regarding the potential health risks a

关键词： Hepatotoxicity Mice Milk Multi-omics Oxidative stress Photo-oxidation

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Repressor elements provide insights into tissue development and phenotypes in pigs

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Zoological Research 2024年第6期45卷 1313-1326页

作者： Yue-Dong Zhang Chao Guo Hang Liu Yun Gao Yongjun Tan Longjian Niu Ligang Wang Lixian Wang David MIrwin Chunhui Hou Zhong-Yin Zhou Ya-Ping Zhang Key Laboratory of Genetic Evolution&Animal Models and Yunnan Key Laboratory of Molecular Biology of Domestic Animals Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650223China Kunming College of Life Science University of Chinese Academy of SciencesKunmingYunnan 650204China State Key Laboratory for Conservation and Utilization of Bio-resources in Yunnan Yunnan UniversityKunmingYunnan 650091China School of Life Science Yunnan UniversityKunmingYunnan 650091China School of Life Sciences Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui 230026China State Key Laboratory of Hybrid Rice Hunan Hybrid Rice Research CenterHunan Academy of Agricultural SciencesChangshaHunan 410125China School of Public Health and Emergency Management Southern University of Science and TechnologyShenzhenGuangdong 518055China Shenzhen Key Laboratory of Cardiovascular Health and Precision Medicine Southern University of Science and TechnologyShenzhenGuangdong 518055China Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation of Ministry of Agriculture Institute of Animal ScienceChinese Academy of Agricultural SciencesBeijing 100081China Department of Laboratory Medicine and Pathobiology University of TorontoToronto M5S 1A8Canada Yunnan Key Laboratory of Biodiversity Information KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases Kunming Institute of ZoologyChinese Academy of SciencesKunmingYunnan 650223China Center for Excellence in Animal Evolution and Genetics Chinese Academy of SciencesKunmingYunnan 650223China

Repressor elements significantly influence economically relevant phenotypes in pigs;however,their precise roles and characteristics are inadequately *** the present study,we employed H3K27me3 profiling,assay for transposase-accessible chromatin with highthroughput sequencing(ATAC-seq),and RNA sequencing(RNA-seq)data across six tissues derived from three embryonic layers to identify and map 2034 super repressor elements(SREs) and 22223 typical repressor elements(TREs) in the pig ***,many repressor elements were conserved across mesodermal and ectodermal *** exhibited tight regulation of their target genes,affecting a limited number of genes within a specific genomic region with pronounced effects,while TREs exerted broader but weaker regulation over a wider range of target ***,in neuronal tissues,genes regulated by repressor elements started to be repressed during the differentiation of stem cells into progenitor ***,analysis showed that many repressor elements exhibited cooperative and additive effects on the modulation of KLF4 *** research provides the first comprehensive map of pig repressor elements,serving as an essential reference for future studies on repressor elements.

关键词： Repressor elements Super repressor elements Silencer Regulatory model Tissue development KLF4 Pig

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Structural and spectroscopic insights into fucoxanthin chlorophyll a/c-binding proteins of diatoms in diverse oligomeric states

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Plant Communications 2024年第11期5卷 128-143页

作者： Cuicui Zhou Yue Feng Zhenhua Li Lili Shen Xiaoyi Li Yumei Wang Guangye Han Tingyun Kuang Cheng Liu Jian-Ren Shen Wenda Wang Photosynthesis Research Center Key Laboratory of PhotobiologyInstitute of BotanyChinese Academy of SciencesBeijing 100093P.R.China University of the Chinese Academy of Sciences Beijing 100049P.R.China China National Botanical Garden Beijing 100093P.R.China Academician Workstation of the Agricultural High-Tech Industrial Area of the Yellow River Delta National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali LandDongying 257300P.R.China Beijing National Laboratory for Condensed Matter Physics Institute of PhysicsChinese Academy of ScienceBeijing 100190P.R.China Research Institute for Interdisciplinary Science Graduate School of Natural Science and TechnologyOkayama UniversityOkayama 700-8530Japan These authors contributed equally to this article

Diatoms,a group of prevalent marine algae,contribute significantly to global primary *** substantial biomass is linked to enhanced absorption of blue-green light underwater,facilitated by fucoxanthin chlorophyll(Chl)a/c-binding proteins(FCPs),which exhibit oligomeric diversity across diatom *** mild clear native PAGE analysis of solubilized thylakoid membranes,we displayed monomeric,dimeric,trimeric,tetrameric,and pentameric FCPs in *** spectrometry analysis revealed that each oligomeric FCP has a specific protein composition,and together they constitute a large Lhcf family of FCP *** addition,we resolved the structures of the Thalassiosira pseudonana FCP(Tp-FCP)homotrimer and the Chaetoceros gracilis FCP(Cg-FCP)pentamer by cryoelectron microscopy at 2.73-Åand 2.65-Åresolution,*** distinct pigment compositions and organizations of various oligomeric FCPs affect their blue-green light-harvesting,excitation energy transfer *** with dimeric and trimeric FCPs,the Cg-FCP tetramer and Cg-FCP pentamer exhibit stronger absorption by Chl c,redshifted and broader Chl a fluorescence emission,and more robust circular dichroism signals originating from Chl a-carotenoid *** spectroscopic characteristics indicate that Chl a molecules in the Cg-FCP tetramer and Cg-FCP pentamer are more heterogeneous than in both dimers and the Tp-FCP *** structural and spectroscopic insights provided by this study contribute to a better understanding of the mechanisms that empower diatoms to adapt to fluctuating light environments.

关键词： diatoms fucoxanthin-chlorophyll a/c-binding proteins oligomeric diversity cryoelectron microscopy light harvesting pigment interaction

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Shallow Sea Bathymetry Mapping from Satellite SAR Observations Using Deep Learning 2

Shallow Sea Bathymetry Mapping from Satellite SAR Observatio...

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2nd IEEE International Conference on Signal, Information and Data Processing, ICSIDP 2024

作者： Cui, Yide Wang, Sheng Du, Yanlei Yu, Yang Liu, Guihong Ma, Wentao Yin, Junjun Yang, Xiaofeng Chinese Academy of Sciences Key Laboratory of Remote Sensing and Digital Earth Aerospace Information Research Institute Beijing China Chinese Academy of Sciences National Key Laboratory of Microwave Imaging Aerospace Information Research Institute Beijing China University of Science and Technology Beijing School of Computer and Communication Engineering Beijing China Nanjing University Institute of Space Earth Science Suzhou China

ISBN: (纸本)9798331515669

This study proposes a deep-learning model to map underwater topography from spaceborne synthetic aperture radar (SAR) images. For the model development, 18 high-resolution Sentinel-1 dual-polarization SAR images covering the southern Cape Cod area from 2014 to 2022 are collected, among which twelve images were used for model training, while the rest were used for testing. In order to facilitate the model learning complex and nonlinear relations among various parameters, the model input is designed to consist of 8 variables, which involve radar backscatter coefficient, radar observation geometry, geography information and marine dynamic environmental parameters. The deep learning network consists of a convolutional layer, two residual blocks, and a fully connected layer, facilitating feature extraction and regression prediction. The final model performances in retrieving shallow water depth are shown as follows: For the training set, the model achieved a root mean square error (RMSE) of 1.02 m and a relative error of 4.32%, with the maximum detectable water depth reaching 48.17 m. For testing, the best performance of the model presented with an RMSE of 1.59 m and a relative error of 5.29%, while the worst performance corresponds to an RMSE of 4.53 m with a relative error of 15.61%. Thus, the proposed model has the capacity of detecting the shallow water depth up to 50 meters with high precision. © 2024 IEEE.

关键词： Mean square error

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