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DCL2-dependent regulation of sRNA biogenesis and translocation in soybean

Crop Journal 2025年第3期13卷 818-827页

作者： Kong, Xiangying Lyu, Kaiwen Xu, Ruixin Zhang, Yan Lu, Dongdong Liu, Yongqi Zhai, Jixian Qiu, Lijuan Li, Bosheng Yan, Zhe Key Laboratory of Soybean Molecular Design Breeding Northeast Institute of Geography and Agroecology Chinese Academy of Sciences Jilin Changchun 130000 China University of Chinese Academy of Sciences Beijing 100000 China The National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI)/State Key Laboratory of Crop Gene Resources and Breeding/Key Laboratory of Crop Gene Resource and Germplasm Enhancement (MOA)/Key Laboratory of Grain Crop Genetic Resources Evaluation and Utilization Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 China Shandong Key Laboratory of Precision Molecular Crop Design and Breeding Peking University Institute of Advanced Agricultural Sciences Shandong Laboratory of Advanced Agricultural Sciences in Weifang Shandong Weifang 261325 China Institute of Plant and Food Science Department of Biology School of Life Sciences Southern University of Science and Technology Guangzhou Shenzhen 518000 China

Small RNAs (sRNAs) are essential for regulating plant growth and development, and they possess the notable ability to travel long distances within organisms to regulate target gene expression. Our study examined the dcl2 mutant, a key enzyme in sRNA biogenesis, to determine the role of the DCL2 protein in sRNA synthesis and to identify mobile sRNAs under DCL2 regulation. Through grafting experiments between dcl2 mutants and wild-type soybean plants, coupled with sRNA sequencing, we identified 14,105 sRNAs significantly affected by DCL2 and discovered 375 mobile sRNAs under its regulation. Degradome analysis provided deeper insights into the regulatory effects of these mobile sRNAs on their target genes, enabling us to understand their potential influences on plant development and stress responses. Leveraging the systemic movement of sRNAs from roots to shoots, we propose a novel strategy for manipulating gene expression in aboveground tissues. Overall, our research findings not only deepen our understanding of the complex regulatory networks involving mobile sRNAs regulated by DCL2, but also provide a new strategy for gene regulation, which could have a positive impact on agricultural biotechnology. © 2025 Crop science Society of China and Institute of Crop science, CAAS

关键词： DCL2-dependent small RNA Graft Mobile small RNAs Soybean

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Lipid metabolism analysis in esophageal cancer and associated drug discovery

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Journal of Pharmaceutical analysis 2024年第1期14卷 1-15页

作者： Ruidi Jiao Wei Jiang Kunpeng Xu Qian Luo Luhua Wang Chao Zhao Bionic Sensing and Intelligence Center Institute of Biomedical and Health EngineeringShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong518000China Department of Radiation Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital&Shenzhen HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeShenzhenGuangdong518116China School of Medicine Southern University of Science and TechnologyShenzhenGuangdong518000China University of Chinese Academy of Sciences Beijing100049China Shenzhen Key Laboratory of Precision Diagnosis and Treatment of Depression Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong518000China

Esophageal cancer is an upper gastrointestinal malignancy with a bleak *** is still being explored in depth due to its complex molecular mechanisms of occurrence and *** play a crucial role in cells by participating in energy supply,biofilm formation,and signal transduction processes,and lipid metabolic reprogramming also constitutes a significant characteristic of malignant *** and more studies have found esophageal cancer has obvious lipid metabolism abnormalities throughout its beginning,progress,and treatment *** inhibition of tumor growth and the enhancement of antitumor therapy efficacy can be achieved through the regulation of lipid ***,we reviewed and analyzed the research results and latest findings for lipid metabolism and associated analysis techniques in esophageal cancer,and comprehensively proved the value of lipid metabolic reprogramming in the evolution and treatment resistance of esophageal cancer,as well as its significance in exploring potential therapeutic targets and biomarkers.

关键词： Lipid metabolism Esophageal cancer Progression Treatment resistance New therapeutic targets

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Biosensing analysis based on Au-Cu bimetallic nanoclusters for colorimetric and fluorescent detection of aflatoxin B1 in cereals and milk

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Food science and Human Wellness 2024年

作者： Hui Deng Songlin Liu Xin Deng Xu Wang Ying Xiong Qian Wen Wang Li Yanni Chen Jiali Ren College of Food Science and Engineering， Central South University of Forestry and Technology National Engineering Research Center of Rice and Byproduct Deep Processing， Central South University of Forestry and Technology Hunan Key Laboratory of Forestry Edible Sources Safety and Processing， Central South University of Forestry and Technology Institute of Quality Standard and Monitoring Technology for Agro-products of Guangdong Academy of Agricultural Sciences Key Laboratory of Testing and Evaluation for Agro-product Safety and Quality， Ministry of Agriculture and Rural Affairs

Aflatoxin B1 (AFB1) in food is a threaten to food safety and human health. In this study， a colorimetric and fluorescent dual-mode aptamer sensor was constructed for rapid and accurate detection of AFB1based on polyvinylpyrrolidone-gold-copper bimetallic nanoclusters （PVP-Au/CuNCs） and Mn3O4nanoparticles (Mn3O4NPs). In the presence of AFB1， the AFB1aptamer on the surface of Mn3O4NPs specifically bound to AFB1. The oxidase-like activity of Mn3O4NPs was restored and 3，3′5，5′-tetramethylbenzidine （TMB） was oxidized to oxidized TMB （oxTMB）， which quenched the orange fluorescence of the PVP-Au/CuNCs. With AFB1concentrations increasing， the blue color of oxTMB deepened， the fluorescence quenching efficiency was enhanced. The working range was 0–56 μg L-1with a limit of detection （LOD） of 0.36 μg L-1（colorimetric）， and 2.86–40 μg L-1with a LOD of 0.29 μg L-1（fluorescent）. In addition， the established sensor was successfully applied to determine AFB1in milk， rice， oats， and corn， with a recovery rate of 91.1%–107.5%. This study provides an insight for fabricating a sensitive， efficient， and accurate analytical platform for rapid detection of AFB1in food.

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Effect of Mariculture on Planktonic Protist:A Case Study in a Marine Ranch of Northern Yellow Sea,China

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Journal of Ocean University of China 2024年第6期23卷 1630-1644页

作者： ZHAO Chenchen ZHANG Xiaoxin ZHANG Wenjing XU Ning ZHANG Xiaoli ZHAO Jianmin ZHANG Qianqian JI Daode School of Ocean Yantai UniversityYantai 264003China Key Laboratory of Coastal Environmental Processes and Ecological Remediation Yantai Institute of Coastal Zone ResearchChinese Academy of SciencesYantai 264003China Muping Coastal Environment Research Station Yantai Institute of Coastal Zone ResearchChinese Academy of SciencesYantai 264003China Laboratory for Marine Biology and Biotechnology Qingdao Marine Science and Technology CenterQingdao 266237China University of Chinese Academy of Sciences Beijing 100049China

Mariculture exerts a direct influence on planktonic eukaryotic microorganisms *** studies have focused on phytoplankton,while the heterotrophic and parasitic protistan groups are ***,in Muping Marine Ranch(MR)which is along the littoral zone of northern Yellow Sea of China,environmental DNA methods were employed to investigate the diversity and seasonal dynamics of near shore planktonic protists in response to *** an integrated analysis of functional groups and a comparison to adjacent sea areas,we found a dominance of harmful algae blooms(HAB)species nearshore MR,and a significant reduction in the diversity of planktonic protists including photosynthetic,heterotrophic and parasitic *** nutrient and low salinity were suggested to drive the dominance of HAB species nearshore MR,which in return might inhibit predators,saprophytes and algal *** investigations revealed an Olisthodiscus luteus outbreak in spring,as well as significant seasonal variations in the planktonic protist community,which might be caused by the changes of *** offshore hypoxia reduced the abundance of phytoplankton but increased mixotrophs and animal parasites of the protistan community nearshore *** study highlights the influence of mariculture on the overall community function of planktonic protists,and is helpful to assess the mariculture's impact on water health.

关键词： mariculture protist hypoxia meta-barcoding harmful algae blooms functional group

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Ecological health assessment of Tibetan alpine grasslands in Gannan using remote sensed ecological indicators

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地球空间信息科学学报（英文版） 2025年第1期28卷中插14,246-264页

作者： Zeyu Du Xibin Ji Jane Liu Wenyue Zhao Zhibin He Jiachang Jiang Qiyue Yang Liwen Zhao Jinlong Gao Linze Inland River Basin Research Station Key Laboratory of Ecological Safety and Sustainable Development in Arid LandsNorthwest Institute of Eco-Environment and ResourceChinese Academy of SciencesLanzhouChina University of Chinese Academy of Sciences BeijingChina Linze Inland River Basin Research Station Key Laboratory of Ecological Safety and Sustainable Development in Arid LandsNorthwest Institute of Eco-Environment and ResourceChinese Academy of SciencesLanzhouChina Academy of Carbon Neutrality Fujian Normal UniversityFuzhouChina Department of Geography and Planning University of TorontoTorontoCanada Gansu Grassland Technical Extension Station LanzhouChina College of Pastoral Agriculture Science and Technology Lanzhou UniversityLanzhouChina

Ecosystem health assessments are crucial to protect the ecological environment and ensure the sustainable ecological functions of alpine *** present,few studies evaluating the ecosystem health of the Gannan alpine grassland,China,an ecologically fragile area,based on a remote sensing theoretical framework *** such,this study assessed the ecosystem health of the Gannan alpine grassland based on the Remote Sensing-based Ecological Index(RSEI)and provided a comparative analysis of the RSEI and Gross Primary Productivity(GPP),extending the study of their spatiotemporal patterns and influencing *** results suggested that RSEI and GPP showed strong comparability in an ecological sense,with the RSEI better reflecting changes in ecosystem health of the Gannan alpine grassland than the ***,the health of the Gannan alpine grassland ecosystem was good(RSEI of 0.61-0.76)and a slow,fluctuating upward trend was seen from 2000(RSEI=0.66)to 2020(RSEI=0.72).Notably,the RSEI was high in the south and low in the north of the *** the past 21 years,43.92%of the ecologically healthy grassland in the southwest of Gannan has been degrading,while the poor ecological health of 39.04%of the grasslands in the southeast and northeast *** model test results show that RSEI could reasonably evaluate the ecosystem health of Gannan alpine *** assessment results provide important scientific data and information on health monitoring and targeted ecological restoration efforts in the Gannan region.

关键词： Ecological health Remote Sensing-based Ecological Index(RSEI) gross primary production remote sensing alpine grassland

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Based on non-targeted metabolomics for differential components screening of Rosae Chinensis Flos and Rosae Rugosae Flos and their quality evaluation

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Traditional Medicine Research 2025年第2期10卷 1-15页

作者： Xu Liang Ni-Hui Zhang Zhi-Lai Zhan Guang-Lu Chang Yan Gao Xia Li Wen-Yuan Gao School of Pharmaceutical Science and Technology Faculty of MedicineTianjin UniversityTianjin 300193China State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs China Academy of Chinese Medical SciencesBeijing 100700China Key Laboratory of Modern Chinese Medicine Resources Research Enterprises Tianjin 300402China

Background:Rosa chinensis *** Rosa rugosa *** not only of ornamental value,but also edible flowers and the flower buds have been listed in the Chinese Pharmacopoeia as traditional *** two plants have some differences in efficacy,but the flower buds are easily confused for similar *** addition,large-scale cultivation of ornamental rose flowers may lead to a decrease in the effective components of medicinal ***,it is necessary to study the chemical composition and make quality evaluation of Rosae Chinensis Flos(Yueji)and Rosae Rugosae Flos(Meigui).Methods:In this study,40 batches of samples including Meigui and Yueji from different regions in China were collected to establish high-performance liquid chromatography ***,the fingerprints data was analyzed using principal component analysis,hierarchical cluster analysis,and partial least squares discriminant analysis analysis chemometrics to obtain information on intergroup differences,and non-targeted metabolomic techniques were applied to identify and compare chemical compositions of samples which were chosen from groups with large *** compounds were screened by orthogonal partial least-squares discriminant analysis and S-plot,and finally multi-component quantification was performed to comprehensively evaluate the quality of Yueji and ***:The similarity between the fingerprints of 40 batches roses and the reference print R was 0.73 to 0.93,indicating that there were similarities and differences between the *** principal component analysis and hierarchical cluster analysis of fingerprints data,the samples from different origins and varieties were intuitively divided into four *** least-squares discriminant analysis analysis showed that Meigui and Yueji cluster into two categories and the model was reliable.A total of 89 compounds were identified by high resolution mass spectrometry,mainly were flavonoids and flavon

关键词： Rosa chinensis Jacq. Rosa rugosa Thunb. metabolomics chemometrics multiple component quantification quality evaluation

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Research on the Failure Mechanism of Pipeline Tee Cracking in Slurry Bed Hydrogenation System

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Journal of Failure analysis and Prevention 2025年 1-13页

作者： Liu, Jiyang Cheng, Mao Yan, Shuangsheng Shen, Zhigang Huang, Guogen Zhejiang Academy of Special Equipment Science Hangzhou China Key Laboratory of Special Equipment Safety Testing Technology of Zhejiang Province Hangzhou China China Special Equipment Inspection & Research Institute Beijing China

The slurry bed hydrogenation process is being increasingly and widely utilized, and corrosion leaks frequently occur in the slurry bed residue hydrogenation unit. In response to the cracking phenomenon in a slurry bed residue hydrogenation, this article analyzed the reasons for the cracking failure of the tee from four aspects: nondestructive testing, mechanical performance analysis, microstructure analysis, and stress analysis, based on the experimental perspective and the finite element simulation method. The results indicate that the three-way pipe fitting (tee) underwent severe high-temperature hydrogen damage and hydrogen embrittlement on the inner wall of the pipeline in a high-temperature hydrogen environment, leading to severe decarburization and microcracks at grain boundaries. Cracking occurred at the shoulder of the three-way fitting, where high-temperature hydrogen corrosion and residual stress concentration were most severe. This study will supplement the database of failure cases of slurry bed residue hydrogenation unit, based on which design optimization and failure prevention will be possible.

关键词： Slurry bed reactor Failure analysis Tee cracking Visualization experiment Simulation

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Pan-omics-based characterization and prediction of highly multidrugadapted strains from an outbreak fungal species complex

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The Innovation 2024年第5期5卷 101-109,100页

作者： Xin Fan Lei Chen Min Chen Na Zhang Hong Chang Mingjie He Zhenghao Shen Lanyue Zhang Hao Ding Yuyan Xie Yemei Huang Weixin Ke Meng Xiao Xuelei Zang Heping Xu Wenxia Fang Shaojie Li Cunwei Cao Yingchun Xu Shiguang Shan Wenjuan Wu Changbin Chen Xinying Xue Linqi Wang Department of Infectious Diseases and Clinical Microbiology Beijing Institute of Respiratory Medicine and Beijing Chao-Yang HospitalCapital Medical UniversityBeijing 100020China Beijing Research Center for Respiratory Infectious Diseases Beijing 100020China State Key Laboratory of Mycology Institute of MicrobiologyChinese Academy of SciencesBeijing 100101China Department of Dermatology Shanghai Key Laboratory of Molecular Medical MycologyChangzheng HospitalShanghai 200003China University of Chinese Academy of Sciences Beijing 100049China Key Laboratory of Intelligent Information Processing Institute of Computing TechnologyChinese Academy of SciencesBeijing 100190China Department of Respiratory and Critical Care Emergency and Critical Care Medical CenterBeijing Shijitan HospitalCapital Medical UniversityBeijing 100038China Department of Laboratory Medicine State Key Laboratory of Complex Severe and Rare DiseasesPeking Union Medical College HospitalChinese Academy of Medical SciencesBeijing 100730China Beijing Key Laboratory for Mechanisms Research and Precision Diagnosis of Invasive Fungal Diseases Beijing 100730China Department of Clinical Laboratory First Affiliated Hospital of Xiamen UniversityXiamen 361003China Institute of Biological Science and Technology Guangxi Academy of SciencesNanning 530007China Department of Dermatology The First Affiliated Hospital of Guangxi Medical UniversityNanning 530021China Guangxi Key Laboratory of Mycosis Prevention and Treatment Nanning 530021China Department of Laboratory Medicine Shanghai East HospitalTongji University School of MedicineShanghai 200120China The Unit of Pathogenic Fungal Infection&Host Immunity Shanghai Institute of Immunity and InfectionChinese Academy of SciencesShanghai 200031China Nanjing Advanced Academy of Life and Health Nanjing 211135China Department of Respiratory and Critical Care Shandong Second Medical UniversityWeifang 261035China

Strains from the Cryptococcus gattii species complex(CGSC)have caused the Pacific Northwest cryptococcosis outbreak,the largest cluster of lifethreatening fungal infections in otherwise healthy human hosts known to *** this study,we utilized a pan-phenome-based method to assess the fitness outcomes of CGSC strains under 31 stress conditions,providing a comprehensive overview of 2,821 phenotype-strain associations within this pathogenic *** clustering analysis revealed a strong correlation between distinct types of stress phenotypes in a subset of CGSC strains,suggesting that shared determinants coordinate their adaptations to various ***,a specific group of strains,including the outbreak isolates,exhibited a remarkable ability to adapt to all three of the most commonly used antifungal drugs for treating cryptococcosis(amphotericin B,5-fluorocytosine,and fluconazole).By integrating pan-genomic and pan-transcriptomic analyses,we identified previously unrecognized genes that play crucial roles in conferring multidrug resistance in an outbreak strain with high multidrug *** these genes,we identified biomarkers that enable the accurate prediction of highly multidrug-adapted CGSC strains,achieving maximum accuracy and area under the curve(AUC)of 0.79 and 0.86,respectively,using machine learning ***,we developed a pan-omic approach to identify cryptococcal multidrug resistance determinants and predict highly multidrug-adapted CGSC strains that may pose significant clinical concern.

关键词： multidrug adapted prediction

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Slope Instability analysis of the Qiongdongnan Basin in the Northern Part of the South China Sea:Implications for the Risk Evaluation of Deepwater Drilling

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Journal of Ocean University of China 2023年第2期22卷 393-409页

作者： MA Linwei WU Shiguo LIU Yanrui SUN Jin OUYANG Min LI Qingping QIN Yongpeng WANG Dawei Laboratory of Marine Geophysics and Georesources Institute of Deep-Sea Science and EngineeringChinese Academy of SciencesSanya 572000China University of Chinese Academy of Sciences Beijing 100049China College of Geophysics Chengdu University of TechnologyChengdu 610059China CNOOC China Limited Zhanjiang BranchZhanjiang 524057China State Key Laboratory of Natural Gas Hydrate CNOOC Research Institute Company LimitedBeijing 100028China

The instability of continental slopes damages marine engineering equipment,such as submarine pipelines,resulting in the generation of tsunamis,which endangers the safety of nearshore ***,research on the instability of continental slopes where submarine landslides usually occur is crucial to the risk evaluation of deepwater *** studies were mainly based on simplified 2D and 3D models,which extend the 2D model applied on submarine slopes with complex *** this study,a numerical model with bathymetric data from the Qiongdongnan Basin was ***,3D slope stability analysis and static and dynamic analyses were *** static analysis found two discussions where slopes are most likely to *** the analysis of different seismic forces,the dynamic result showed that an instability area is added to the two positions where the static analysis is *** scatters and transmits seismic waves and controls the accumulation and diffusion of seismic energy.3D calculations and analysis revealed that the direction of slope instability is closely related to terrain inclination,slope,terrain effect,and terrain *** showed that instability situations could not be derived from a single direction or profile *** situations are an important factor in slope stability analysis and are critical to the prediction and evaluation of marine geological disasters.

关键词： South China Sea submarine landslide slope instability topography curvature topography slope granite

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Research Progress of Lithium Plating on Graphite Anode in Lithium-Ion Batteries

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Chinese Journal of Chemistry 2021年第1期39卷 165-173页

作者： Daozhong Hu Lai Chen Jun Tian Yuefeng Su Ning Li Gang Chen Yulu Hu Yueshan Dou Shi Chen Feng Wu Beiing Key Laboratory of Environmental Science and Engineering School of Materials Science and EngineeringBeijing Institute of TechnologyBeijing 100081China Department of Testing Technology China North Vehicle Research InstituteBeijing 100072China Beijing Institute of Technology Chongqing Innovation Center Chongqing 401120China

Lithium plating on graphite anode is triggered by harsh conditions of fast charge and low temperature,which sig-nificantly accelerates SOH(state of health)degradation and may cause safety issues of lithium ion batteries(LIBs).This paper has reviewed recent research progress of lithium plating on graphite ***,we summarize the forming mechanisms of Li plating with(corresponding influence factors,the detect-ing methods and hazard of Li ***,approaches to suppress Li plating are discussed,including anode surface modification,electrolyte composition optimization and development of optimal ***,we con-clude and propose the remaining challenges and prospects in terms of mechanism research,detecting approaches,and suppressing methods of Li *** review highlights the development of Li plating research and plays a guiding rule of further study on Li plating in LIBs.

关键词： Lithium Plating Graphite anode Electron microscopy Low-temperature physics

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