检索结果-内蒙古大学图书馆

Customizable Automated High Content Image Acquisition and Analysis for Tissue Biopsies

Microscopy Today 2022年第2期30卷 30-38页

作者： Neal, Christopher A Hodge, Jacob G Zamierowski, David S Mellott, Adam J KIDDRC Imaging Core Facility University of Kansas Medical Center Kansas City KS Bioengineering Graduate Program University of Kansas Lawrence KS Department of Plastic Surgery University of Kansas Medical Center Kansas City KS

Advancements in high content image analysis have led to an increase in the adoption of these techniques in basic science and clinical research. High-throughput approaches to imaging and image analysis require minimal user interventions, circumventing the often prohibitively time-consuming and unreliable standard manual analysis. In this study, we demonstrate how high content imaging (HCI) techniques, in combination with high content analysis (HCA), can be paired with more traditional manual analysis to quantify both micro- and macro-level features of biopsied tissue sections. High-resolution, full-color images of stained tissue were acquired and stitched together to reconstruct the entire tissue section, which enabled analyses that required accurate identification of a given region's location within the tissue section. A custom region of interest grid was generated that followed the curvature of the tissue. The composite images were used in two separate analyses: tissue layer thickness as a macro-level approach, and nuclei density as a micro-level approach. Ultimately, the flexibility of the HCI and HCA methodologies used in this study allowed for complex analysis of tissue that would not have been otherwise feasible.

关键词： high content analysis analytical imaging automated analysis microscopy tissue microanalysis

来源：评论

学校读者我要写书评

暂无评论

Generation of multi-tracer PET attenuation maps using zero-shot learning

Generation of multi-tracer PET attenuation maps using zero-s...

引用

IEEE Symposium on Nuclear Science (NSS/MIC)

作者： M. J. Cho H. S. Shim J. S. Lee Interdisciplinary Program of Bioengineering Seoul National University Seoul Republic of Korea Graduate School Integrated Major in Innovative Medical Science Seoul National University Seoul Republic of Korea Department of Nuclear Medicine Seoul National University Seoul Republic of Korea

Attenuation correction is crucial for achieving high accuracy in PET images. Unfortunately, attenuation correction cannot be performed on PET images when transmission or CT data is unavailable. In such cases, an attenuation map is obtained by utilizing additional anatomical or morphological images. In previous studies, specific models for each tracer in PET images were trained to generate attenuation maps. However, this approach is associated with two major limitations. First, computational resources are greatly increased as each tracer requires a separate network for training. Second, PET images with less commonly used tracers usually have a shortage of data, making it challenging to train a network. To address these limitations, we propose a zero-shot learning method for generating CT-derived attenuation maps (µ-CT) from MLAA-derived attenuation maps (µ-MLAA) using only 18 F-FDG images. Unlike tracer-specific methods which require a separate network for each tracer, this method utilizes a single generator network that encodes the structural features of the input image and the style of the reference µCT to generate the attenuation map. We evaluated our method using clinical brain PET/CT scan datasets of three different tracers, which are 18 F-FDG, 18 F-Florbetaben, and 18 F-FP-CIT. The proposed method which was exclusively trained on 18 F-FDG performs better than tracer-specific networks trained on the same tracer across all evaluation metrics. Moreover, the proposed method generates predictions for the tracers 18 F-Florbetaben and 18 F-FP-CIT, which were not included in the training data, that are either highly comparable or not significantly different from those produced by the tracer-specific networks trained on the corresponding tracer data. The results demonstrate the effectiveness of our zero-shot learning approach in generating multi-tracer PET attenuation maps with only 18 F-FDG.

关键词：

来源：评论

学校读者我要写书评

暂无评论

In vivo Detection of NO using Photonic Microring Resonators

In vivo Detection of NO using Photonic Microring Resonators

引用

Conference on Lasers and Electro-Optics (CLEO)

作者： Sakib Hassan Xuan Zhao Christian C. Schreib Guillaume Duret Omid Veiseh Jacob T. Robinson Electrical & Computer Engineering Rice University Houston USA Applied Physics Graduate Program Smalley-Curl Institute Rice University Houston USA Department of Bioengineering Rice University Houston USA

Here we show how photonic microring resonators enable real-time detection of Nitric Oxide (NO) in vivo, which is critical for physiological monitoring particularly during the wound healing process.

ISBN: (数字)9781957171050

ISBN: (纸本)9781665466660

Here we show how photonic microring resonators enable real-time detection of Nitric Oxide (NO) in vivo, which is critical for physiological monitoring particularly during the wound healing process.

关键词： Integrated optics In vivo Optical resonators Wounds Laser modes Lab-on-a-chip Real-time systems

来源：评论

学校读者我要写书评

暂无评论

Detection of proteoforms from single cells by multiplexed ion beam imaging 24

Detection of proteoforms from single cells by multiplexed io...

引用

24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020

作者： Lomeli, Gabriela Bosse, Marc Bendall, Sean C. Angelo, Michael Herr, Amy E. UC Berkeley UCSF Joint Graduate Program in Bioengineering CA United States Department of Bioengineering University of California Berkeley United States Department of Pathology Stanford University StanfordCA United States

ISBN: (纸本)9781733419017

Single-cell electrophoretic assays offer proteoform detection specificity, but target multiplexing can be further enhanced by transitioning from fluorescence to mass spectrometry imaging for readout. We report for the first time multiplexed ion beam imaging (MIBI) of isoelectrically separated proteoforms from single cells (scIEF). MIBI of scIEF enables the detection of proteoforms differing by a single charge unit. Additionally, we demonstrate that MIBI's ability to do multiple depth image acquisition-comparable to z-stack confocal imaging-enables us to disregard surface artefacts that would normally confound quantitation. © 2020 CBMS-0001

关键词： Depth profiling

来源：评论

学校读者我要写书评

暂无评论

Miniscope3D:optimized single-shot miniature 3D fluorescence microscopy

引用

Light(Science & Applications) 2020年第1期9卷 367-379页

作者： Kyrollos Yanny Nick Antipa William Liberti Sam Dehaeck Kristina Monakhova Fanglin Linda Liu Konlin Shen Ren Ng Laura Waller UCB/UCSF Joint Graduate Program in Bioengineering University of CaliforniaBerkeleyCA 94720USA Department of Electrical Engineering&Computer Sciences University of CaliforniaBerkeleyCA 94720USA TIPs Department Universite libre de Bruxelles(ULB)1050 BrusselsBelgium

Miniature fluorescence microscopes are a standard tool in systems ***,widefield miniature microscopes capture only 2D information,and modifications that enable 3D capabilities increase the size and weight and have poor resolution outside a narrow depth ***,we achieve the 3D capability by replacing the tube lens of a conventional 2D Miniscope with an optimized multifocal phase mask at the objective’s aperture *** the phase mask at the aperture stop significantly reduces the size of the device,and varying the focal lengths enables a uniform resolution across a wide depth *** phase mask encodes the 3D fluorescence intensity into a single 2D measurement,and the 3D volume is recovered by solving a sparsity-constrained inverse *** provide methods for designing and fabricating the phase mask and an efficient forward model that accounts for the fieldvarying aberrations in miniature *** demonstrate a prototype that is 17mm tall and weighs 2.5 grams,achieving 2.76μm lateral,and 15μm axial resolution across most of the 900×700×390μm^(3) volume at 40 volumes per *** performance is validated experimentally on resolution targets,dynamic biological samples,and mouse brain *** with existing miniature single-shot volume-capture implementations,our system is smaller and lighter and achieves a more than 2×better lateral and axial resolution throughout a 10×larger usable depth *** microscope design provides single-shot 3D imaging for applications where a compact platform matters,such as volumetric neural imaging in freely moving animals and 3D motion studies of dynamic samples in incubators and lab-on-a-chip devices.

关键词： enable resolution narrow

来源：评论

学校读者我要写书评

暂无评论

Optical attenuators extend dynamic range but alter angular response of planar ultraviolet-C dosimeters

arXiv

引用

arXiv 2021年

作者： Su, Alison Geldert, Alisha Grist, Samantha M. Herr, Amy E. University of California Berkeley-University of California San Francisco Graduate Program in Bioengineering BerkeleyCA94720 United States Department of Bioengineering BerkeleyCA94720 United States

A challenge for sensors used in ultraviolet-C (UV-C) decontamination protocols of N95 respirators is validation that the entire N95 surface receives the minimum acceptable dose. Photochromic indicators (PCIs) can accurately measure UV-C dose on nonplanar surfaces, but often saturate below doses required to decontaminate porous, multilayered textiles such as N95s. Here, we investigate the use of optical attenuators to extend PCI dynamic range while maintaining a near-ideal angular response - critical for accurate measurements when UV-C is uncollimated. Through an analytical model, we show that tuning attenuator refractive index, attenuation coefficient, and thickness can extend dynamic range, but compromises ideal angular response unless the attenuator is an ideal diffuser. To demonstrate this tradeoff empirically, we pair PCIs with model specular (floated borosilicate) and diffuse (polytetrafluoroethylene) attenuators, characterize the angular response, and evaluate on-N95 UV-C dose measurement accuracy of each PCI-attenuator stack in a UV-C decontamination system. While both borosilicate and polytetrafluoroethylene increase PCI dynamic range >4×, both attenuators introduce angle-dependent transmittance, which causes location-dependent underestimation of UV-C dose. The PCI-borosilicate and PCI-polytetrafluoroethylene stacks underreport true on-N95 dose by 1) 14.7% and 3.6%, respectively, on a surface near-normal to the array of source lamps, and 2) 40.8% and 19.8%, respectively, on a steeply sloped location. Overall, we demonstrate that while planar optical attenuators can increase PCI dynamic range, verification of near-ideal angular response is critical for accurate UVC dose measurement. © 2021, CC BY-NC-SA.

关键词： Refractive index

来源：评论

学校读者我要写书评

暂无评论

BPS2025 - Characterization of intrinsically disordered protein-polymer interactions with fluorescence polarization

引用

Biophysical Journal 2025年第3期124卷 164a-164a页

作者： Wesley W. Leung Wenchang Zhou Julea Vlassakis Applied Physics Graduate Program Rice University Houston TX USA Department of Bioengineering Rice University Houston TX USA

来源：评论

学校读者我要写书评

暂无评论

Engineering FRET biosensor for H3K9 acetylation imaging in single living cells

引用

Med-X 2024年第1期2卷 1-16页

作者： Li, Shitian Sun, Chang Harrison, Reed E. S. Cheng, Leonardo Gao, Li Liu, Longwei Wang, Yingxiao Peng, Qin Department of Bioengineering Institute of Engineering in Medicine University of California San Diego USA Biological Sciences Graduate Program University of California San Diego USA Institute of Systems and Physical Biology Shenzhen Bay Laboratory Shenzhen China School of Chemical Engineering and Technology North University of China Taiyuan China Alfred E. Mann Department of Biomedical Engineering University of Southern California Los Angeles USA

Histone acetylation is an important epigenetic modification that governs gene expression, chromatin changes in stress response, and cell fate transition. FRET biosensors have been developed for various epigenetic events to enable spatiotemporal tracking of sub-cellular signaling events. Previously reported histone H3 acetylation biosensor recognizing two acetyl residues lacked specificity. In this study, using a single bromodomain of the BRD4, we have developed a genetically encoded H3K9ac biosensor. We systematically investigated different combinations of the BET family protein as binding domains and performed site-saturated mutagenesis to optimize the biosensor, achieving a dynamic FRET change up to 30% under TSA treatment. With the application of the optimized H3K9ac biosensor, we revealed different basal active chromatin architectures in invasive tumor cells compared to benign tumor cells. Furthermore, we found that H3K9ac level increased dramatically when cancer cells passed through microchannels, which models the physical constraints and mechanical microenvironmental conditions that cancer cells encounter when passing through narrow spaces within the body. This result highlights the chromatin plasticity in response to external mechanical stresses. In summary, our H3K9ac biosensor provides a versatile tool for mechanistic investigation of cell fate transition in cancer and mechanotransduction.

关键词： FRET biosensor H3K9 acetylation Nuclear deformation Cancer cell migration

来源：评论

学校读者我要写书评

暂无评论

Droplet digital quantification of nucleic acids with Cas13a 25

Droplet digital quantification of nucleic acids with Cas13a

引用

25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021

作者： Liu, Frank X. Cui, Johnson Q. Yao, Shuhuai Department of Mechanical and Aerospace Engineering The Hong Kong University of Science and Technology Clear Water Bay Kowloon Hong Kong Bioengineering Graduate Program Department of Chemical and Biological Engineering The Hong Kong University of Science and Technology Clear Water Bay Kowloon Hong Kong

ISBN: (纸本)9781733419031

The isothermal amplification coupled with CRISPR detection has attracted tremendous interest for the sensitive, specific, and qualitative detection of nucleic acids. We report here a Microfluidics Enabled Digital Isothermal CRISPR-cas13a Assay (MEDICA) which leverages the droplet microfluidics technology to enable recombinase polymerase amplification (RPA) and Cas13a trans-cleavage reporting in individual droplet partitions for absolute quantification. The optimal one-pot MEDICA achieved single nucleic acid molecule detection within 30 min. We applied MEDICA to quantify HPV 16 and 18 viral loads in clinical samples, indicating complete accordance with quantitative polymerase chain reaction (qPCR) results. © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.

关键词： Drops

来源：评论

学校读者我要写书评

暂无评论

MICROFLUIDIC PLATFORM FOR MULTI-FREQUENCY VISCOELASTIC PHENOTYPING OF SINGLE CELLS 25

MICROFLUIDIC PLATFORM FOR MULTI-FREQUENCY VISCOELASTIC PHENO...

引用

25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021

作者： Lai, Andre Dong, Alan Lustig, Michael Sohn, Lydia L. UC Berkeley-UC San Francisco Graduate Program in Bioengineering University of California BerkeleyCA United States Electrical Engineering and Computer Sciences University of California BerkeleyCA United States Department of Mechanical Engineering University of California BerkeleyCA United States

ISBN: (纸本)9781733419031

Biomechanics plays an integral role in cancer development and progression. Accurately quantifying the mechanical properties of cells would enable a more comprehensive understanding of cancer, complementing conventional biochemical and genetic analyses. Here, we introduce a microfluidic platform for measuring single cell viscoelasticity at multiple frequencies in a high-throughput and label-free manner. Using the Coulter principal and a unique microfluidic channel geometry, our device records resistance pulses in order to estimate a cell's viscoelastic phenotype. We demonstrate our platform's capabilities by measuring three breast epithelial cell lines. © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.

关键词： Biomechanics

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：