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Development of exosome membrane materialsfused microbubbles for enhanced stability and efficient drug delivery of ultrasound contrast agent

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Acta Pharmaceutica Sinica B 2023年第12期13卷 4983-4998页

作者： Yongho Jang Jeehun Park Pilsu Kim Eun-Joo Park Hyungjin Sun Yujin Baek Jaehun Jung Tai-kyong Song Junsang Doh Hyuncheol Kim Department of Chemical and Biomolecular Engineering Sogang UniversitySeoul 04107Republic of Korea Research Institute of Advanced Materials(RIAM) Institute of Engineering ResearchSeoul National UniversitySeoul 08826Republic of Korea Department of Electronic Engineering Sogang UniversitySeoul 04107Republic of Korea Biomedical Research Institute&Department of Radiology Seoul National University HospitalSeoul 03080Republic of Korea Interdisciplinary Program in Bioengineering Seoul National UniversitySeoul 08826Republic of Korea Department of Materials Science and Engineering Seoul National UniversitySeoul 08826Republic of Korea Institute of Engineering Research Bio-MAX InstituteSeoul National UniversitySeoul 08826Republic of Korea Department of Biomedical Engineering Sogang UniversitySeoul 04107Republic of Korea

Lipid-coated microbubbles are widely used as an ultrasound contrast agent,as well as drug delivery ***,the two main limitations in ultrasound diagnosis and drug delivery using microbubbles are the short half-life in the blood system,and the difficulty of surface modification of microbubbles for active *** exosome,a type of extracellular vesicle,has a preferentially targeting ability for its original *** this study,exosome-fused microbubbles(Exo-MBs)were developed by embedding the exosome membrane proteins into *** a result,the stability of Exo-MBs is improved over the conventional *** the same principle that under the exposure of ultrasound,microbubbles are cavitated and self-assembled into nano-sized particles,and Exo-MBs are self-assembled into exosome membrane proteins-embedded nanoparticles(Exo-NPs).The Exo-NPs showed favorable targeting properties to their original cells.A photosensitizer,chlorin e6,was loaded into Exo-MBs to evaluate therapeutic efficacy as a drug *** higher therapeutic efficacy of photodynamic therapy was confirmed,followed by cancer immunotherapy from immunogenic cell *** have therefore developed a novel ultrasound image-guided drug delivery platform that overcomes the shortcomings of the conventional ultrasound contrast agent and is capable of simultaneous photodynamic therapy and cancer immunotherapy.

关键词： Exosomes Microbubbles Photodynamic therapy Ultrasound Sonoporation Cancer immunotherapy Drug delivery

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Characterization of the complete mitochondrial genome ofChionobathyscus dewitti(Perciformes, Channichthyidae)

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Mitochondrial DNA Part B 2019年第2期4卷 3914-3915页

作者： Sapto Andriyono Md. Jobaidul Alam Soo Rin Lee Seok-Gwan Choi Sangdeok Chung Hyun-Woo Kim Interdisciplinary Program of Biomedical Mechanical and Electrical Engineering Pukyong National University Busan Republic of KoreaFisheries and Marine Faculty Universitas Airlangga Surabaya Indonesia Interdisciplinary Program of Biomedical Mechanical and Electrical Engineering Pukyong National University Busan Republic of Korea National Institute of Fisheries Science (NIFS) Busan Republic of Korea Interdisciplinary Program of Biomedical Mechanical and Electrical Engineering Pukyong National University Busan Republic of KoreaDepartment of Marine Biology Pukyong National University Busan Republic of Korea

The complete mitochondrial genome sequence of the Icefish,Chionobathyscus dewittiwas determined by the Next Generation Sequencing (NGS) analysis. The complete mitogenome was 17,452 bp in length, which encoded the canonical 13 protein-coding genes, 22 tRNAs, two rRNAs, and two non-coding regions. As shown in the other notothenids, translocation of ND6 and an additional non–coding region were identified, which is different from the typical vertebrate mitochondrial genomes. TheC. dewittiwas clustered distinctly from the those in theChinodracoandChaenocephalus, which supported the idea that this species should be classified in the different genus,Chionobathyscusin the family Channichthyidae.

关键词： Icefish next-generation sequencing Chionobathyscus dewitti Antarctic

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Circularly polarized luminescent self-organized helical superstructures:From materials and stimulus-responsiveness to applications

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Aggregate 2021年第6期2卷 60-81页

作者： Yanrong He Siyang Lin Jinbao Guo Quan Li Key Laboratory of Carbon Fibers and Functional Polymers Ministry of Educationand College of Materials Science and EngineeringBeijing University of Chemical TechnologyBeijingChina Institute of Advanced Materials School of Chemistry and Chemical Engineeringand Jiangsu Province Hi-Tech Key Laboratory for Biomedical ResearchSoutheast UniversityNanjingChina Advanced Materials and Liquid Crystal Institute and Chemical Physics Interdisciplinary Program Kent State UniversityKentOhioUSA

Circularly polarized luminescence(CPL)has gained considerable attention in various systems and has rapidly developed into an emerging research *** meet the needs of actual applications in diverse fields,a high luminescence dissymmetry factor(glum)and tunable optical performance of CPL would be the most urgent pursuit for ***,many emerging CPL materials and various strategies have been developed to address these critical *** cholesteric liquid crystals(CLCs),that is,luminescent self-organized helical superstructures,are considered to be ideal candidates for constructing CPL-active materials,as they not only exhibit high glum values,but also enable flexible optical control of *** review mainly summarizes the characteristics of CPL based on CLCs as the bulk phase doped with different emitters,including aggregated induced emission molecules,conventional organic small molecules,polymer emitters,metal-organic complex emitters,and luminescent *** addition,the recent significant progress in stimulus-responsive CPL based on emissive CLCs in terms of several types of stimuli,including light,electricity,temperature,mechanical force,and multiple stimuli is ***,a short perspective on the opportunities and challenges associated with CPL-active materials based on the CLC field is *** review is anticipated to offer new insights and guidelines for developing CLC-based CPL-active materials for broader applications.

关键词： amplifying dissymmetry factor cholesteric liquid crystal circularly polarized luminescence self-organized helical superstructure stimulus-responsiveness

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Appropriate feeding for early juvenile stages of eunicid polychaete Marphysa sanguinea

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Fisheries and Aquatic sciences 2017年第1期20卷 1-9页

作者： Kim, Kyeong Hun Kim, Byoung Kwon Kim, Sung Kyun Phoo, War War Venmathi Maran, B.A. Kim, Chang-Hoon Pukyong National University Interdisciplinary Program of Biomedical Engineering Busan 48513 South Korea Pukyong National University Department of Marine Bio-materials and Aquaculture Busan 48513 South Korea Pukyong National University Fisheries Science and Technology Centre Goseong-gun Gyeongsangnam-do 52957 South Korea

Survival rate (SR) and growth rate (GR) were tested with various feed sources to identify an appropriate feed to improve the productivity in the early life stage of the rock worm Marphysa sanguinea (Montagu, 1813) (Eunicidae: Polychaeta). In addition, feed supply rates were also examined. Three experiments were performed to identify the appropriate feed for the juvenile stages of M. sanguinea. Experiment 1 was done using seven different feed sources and without feed as well for the first 20days of M. sanguinea culture. Decapsulated Artemia and extruded pellet for shrimp were showed with high SR and GR in the experiment 1. Experiment 2 was performed with five different feed sources. Two feeds were selected from experiment 1 in addition to eel feed, mixed micro-algae, and benthic diatom. Four different quantities of each feed were supplied to 3000 individuals of early juvenile stage of M. sanguinea. High quantity of decapsulated Artemia and shrimp feed resulted with a relatively good SR and GR. In experiment 3, we provided 20, 50, and 75mg of decapsulated Artemia and shrimp feed to 3000 individuals of M. sanguinea. Our results demonstrated that after 3months, decapsulated Artemia showed high survival rate and 75mg/3000 inds provided the best quantity of feed in the earlier life stage culture of M. sanguinea. © 2017 The Author(s).

关键词： Decapsulated Artemia Feed Feeding rate Marphysa sanguinea Polychaete

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Application of an elastic biodegradable poly(L-lactide-co-Ε- caprolactone) scaffold for cartilage tissue regeneration

Application of an elastic biodegradable poly(L-lactide-co-Ε...

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作者： Jung, Youngmee Kim, Soo Hyun You, Hee Jin Kim, Sang-Heon Ha Kim, Young Min, Byoung Goo Biomaterials Research Center Korea Institute of Science and Technology P.O. Box 131 Cheongryang Seoul 136-650 Korea Republic of Interdisciplinary Program in Medical and Biological Engineering Major Seoul National University Seoul Korea Republic of Department of Materials Science and Engineering Gwangju Institute of Science and Technology Gwangju Korea Republic of Department of Biomedical Engineering Seoul National University Seoul Korea Republic of

In cartilage tissue regeneration, it is important that an implant inserted into a defect site can maintain its mechanical integrity and endure stress loads from the body, in addition to being biocompatible and able to induce tissue growth. These factors are crucial in the design of scaffolds for cartilage tissue engineering. We developed an elastic biodegradable scaffold from poly(L-lactideco-Ε-caprolactone) (PLCL) for application in cartilage treatment. Biodegradable PLCL co-polymer was synthesized from L-lactide and Ε-caprolactone in the presence of stannous octoate as a catalyst. A highly elastic PLCL scaffold was fabricated by a gel-pressing method with 80% porosity and 300-500 μm pore size. The tensile mechanical and recovery tests were performed in order to examine mechanical and elastic properties of the PLCL scaffold. They could be easily twisted and bent and exhibited almost complete (over 94%) recoverable extension up to breaking point. For examining cartilaginous tissue formation, rabbit chondrocytes were seeded on scaffolds. They were then cultured in vitro for 5 weeks or implanted in nude mice subcutaneously. From in vitro and in vivo tests, the accumulation of extracellular matrix on the constructs showed that chondrogenic differentiation was sustained onto PLCL scaffolds. Histological analysis showed that cells onto PLCL scaffolds formed mature and well-developed cartilaginous tissue, as evidenced by chondrocytes within lacunae. From these results, we are confident that elastic PLCL scaffolds exhibit biocompatibility and as such would provide an environment where cartilage tissue growth is enhanced and facilitated. © 2008 VSP.

关键词： Tissue regeneration

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Cartilaginous tissue formation using human adipose tissue derived stromal cells and TGF-β1 loaded functional nanoparticle-hydrogel-PLCL complex

Cartilaginous tissue formation using human adipose tissue de...

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8th World Biomaterials Congress 2008, WBC 2008

作者： Jung, Youngmee Kim, Soo Hyun Chung, Yong-Il Tae, Giyoong Kim, Sang-Heon Kim, Young Ha Lee, Jong Won Min, Byoung Goo Biomaterials Research Center Korea Institute of Science and Technology Seoul Korea Republic of Interdisciplinary Program in Medical and Biological Engineering Major Seoul National University Seoul Korea Republic of Department of Materials Science and Engineering Gwangju Institute of Science and Technology Gwangju Korea Republic of Department of Plastic Surgery Catholic University of Korea Seoul Korea Republic of Department of Biomedical Engineering Seoul National University Seoul Korea Republic of

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Sleep Stage Scoring by EEG Spectral Information Based on Fuzzy Clustering Only

Sleep Stage Scoring by EEG Spectral Information Based on Fuz...

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The 4th ASRS Congress

作者： Jung-Su Oh Jin Woo Seo Byunghun Choi Do-Un Jeong Kwang-Suk Park Interdisciplinary Program-Biomedical Engineering Major Graduate School Seoul National University Interdisciplinary Program-Biomedical Engineering Major Graduate School Seoul National University Interdisciplinary Program-Biomedical Engineering Major Graduate School Seoul National University Department of Psychiatry and Behavioral Science Seoul National University College of Medicine and Division of Sleep Studies Seoul National University Hospital Department of Biomedical Engineering Seoul National University College of Medicine Seoul Republic of Korea

The quantity and quality of human sleep draw more attention as the society becomes more sophisticated. The unique and common method for evaluating human sleep is polysomnography (PSG) and it generates sleep architecture with functional variables. Sleep stages are nonuniform constituents of sleep architecture and each 30-second epoch should be scored accu-

关键词： EOG EMG Sleep Stage Scoring by EEG Spectral Information Based on Fuzzy Clustering Only Fuzzy

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Fluorescence resonance energy transfer detection of mouse immunoglobulin G and Octamer-4 on protein nanoarray

Fluorescence resonance energy transfer detection of mouse im...

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IEEE/ICME International Conference on Complex Medical engineering, CME

作者： Phat. L. Tran Yee Tchao Jeong-Yeol Yoon Biomedical Engineering Graduate Interdisciplinary Program University of Arizona Tucson Tucson AZ USA Department of Veterinary Science and Microbiology University of Arizona Tucson Tucson AZ USA Department of Agricultural and Biosystems Engineering and Biomedical Engineering Graduate Interdisciplinary Program University of Arizona Tucson Tucson AZ USA

An alternative approach for fabricating a protein array at nanoscale (<100 nm) is suggested with a capability of characterization and/or localization of multiple components on a nanoarray. Basically, fluorescent micro- and nanospheres each conjugated with different proteins are size-dependently self-assembled (SDSA) onto these nanometer wells that were created on the polymethyl methacrylate (PMMA) substrate by electron beam lithography (EBL). Particles of different diameters are added serially, and electrostatically attached to the corresponding wells through electrostatic attraction between the carboxylic groups of the spheres and p-doped silicon substrate underneath the PMMA layer. Target detection utilizes fluorescence resonance energy transfer (FRET) from fluorescent beads to target (mouse immunoglobulin G = mIgG or Octamer-4 = Oct4) and its antibody bound on the beads. The 180 nm blue beads are conjugated with mIgG to capture anti-mIgG-FITC. The 50 nm green and 100 nm yellow-green beads are conjugated with anti-Oct4 to capture Oct4 peptides; where the secondary anti-Oct4 tagged with phycoerythrin via F(ab)2 fragment is then added to function as an indicator of Oct4 detection. These protein-conjugated particles are added serially from the largest to the smallest and the particles are successfully self-assembled to the respective nanometer wells to achieve size-dependent self-assembly. FRET signals are detected through fluorescence and confocal microscopes, and further confirmed by Fluorolog3 spectrofluorometer. Therefore, SDSA is a valuable approach for the fabrication of multiple components array; and FRET is a useful biorecognition technique for the detection of mIgG, Oct4 or other targets of interest.

关键词： Fluorescence Resonance Energy exchange Mice Proteins Self-assembly Immune system Electron beams Lithography Electrostatics

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Characterization of the complete mitochondrial genome ofBothrocara hollandi(Perciformes: Zoarcidae)

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Mitochondrial DNA Part B 2019年第1期4卷 523-524页

作者： Nack-Keun Kim Sapto Andriyono Jobaidul Alam Sung-Tae Kim Kyung Mi Jung Hyun-Woo Kim Interdisciplinary Program of Biomedical Mechanical and Electrical Engineering Pukyong National University Busan Republic of Korea Interdisciplinary Program of Biomedical Mechanical and Electrical Engineering Pukyong National University Busan Republic of KoreaFisheries and Marine Faculty Universitas Airlangga Surabaya Indonesia Dokdo Fisheries Research Center National Institute of Fisheries Science Pohang Republic of Korea Interdisciplinary Program of Biomedical Mechanical and Electrical Engineering Pukyong National University Busan Republic of KoreaDepartment of Marine Biology Pukyong National University Busan Republic of Korea

The full mitogenome ofBothrocara hollandiwas determined by high-throughput sequencing (HTS) platform. The genome was 16,732 bp in length, which included 13 protein-coding genes, 22 tRNAs, two ribosomal RNAs (12S and 16S), and a control region (D-Loop). The overall composition of G + C in the mitogenome was 45.90%, which was lower than A + T contents (54.10%). Except for COX1 (GTG), the other twelve protein-coding genes begin with typical start codon, ATG. The incomplete stop codon (TA − or T–) was identified in five genes, including COXI, COXII, COXIII, ND3, and ND4. The phylogenetic analysis with the currently reported mitogenome sequences showed thatB. hollandiwas most closely related toLycodes tanakae, suggesting that additional mitogenome sequences in genusBothrocaraare required for its clear evolutional relationship.

关键词： High-throughput sequencing Bothrocara hollandi mitogenome phylogenetic eelpout

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PD-1 blockade-unresponsive human tumor-infiltrating CD8^(+)T cells are marked by loss of CD28 expression and rescued by IL-15

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Cellular & Molecular Immunology 2021年第2期18卷 385-397页

作者： Kyung Hwan Kim Hong Kwan Kim Hyung-Don Kim Chang Gon Kim Hoyoung Lee Ji Won Han Seong Jin Choi Seongju Jeong Minwoo Jeon Hyunglae Kim Jiae Koh Bo Mi Ku Su-Hyung Park Myung-Ju Ahn Eui-Cheol Shin Graduate School of Medical Science and Engineering Korea Advanced Institute of Science and TechnologyDaejeonRepublic of Korea Department of Thoracic and Cardiovascular Surgery Samsung Medical CenterSungkyunkwan University School of MedicineSeoulRepublic of Korea Biomedical Science and Engineering Interdisciplinary Program Korea Advanced Institute of Science and TechnologyDaejeonRepublic of Korea Research Institute for Future Medicine Samsung Medical CenterSungkyunkwan University School of MedicineSeoulRepublic of Korea Department of Radiation Oncology Yonsei Cancer CenterYonsei University College of MedicineSeoulRepublic of Korea Division of Hematology-Oncology Department of MedicineSamsung Medical CenterSungkyunkwan University School of MedicineSeoulRepublic of Korea

Blockade of programmed death-1(PD-1)reinvigorates exhausted CD8^(+)T cells,resulting in tumor regression in cancer ***,reinvigoration of exhausted CD8^(+)T cells following PD-1 blockade was shown to be CD28-dependent in mouse ***,we examined the role of CD28 in anti-PD-1 antibody-induced human T cell reinvigoration using tumor-infiltrating CD8^(+)T cells(CD8^(+)TILs)obtained from non-small-cell lung cancer ***-cell analysis demonstrated a distinct expression pattern of CD28 between mouse and human CD8^(+)***,we found that human CD28^(+)CD8^(+)but not CD28^(–)CD8^(+)TILs responded to PD-1 blockade irrespective of B7/CD28 blockade,indicating that CD28 costimulation in human CD8^(+)TILs is dispensable for PD-1 blockade-induced reinvigoration and that loss of CD28 expression serves as a marker of anti-PD-1 antibody-unresponsive CD8^(+)*** and phenotypically,PD-1 blockade-unresponsive human CD28^(–)PD-1^(+)CD8^(+)TILs exhibited characteristics of terminally exhausted CD8^(+)T cells with low TCF1 ***,CD28^(–)PD-1^(+)CD8^(+)TILs had preserved machinery to respond to IL-15,and IL-15 treatment enhanced the proliferation of CD28^(–)PD-1^(+)CD8^(+)TILs as well as CD28^(+)PD-1^(+)CD8^(+)*** together,these results show that loss of CD28 expression is a marker of PD-1 blockade-unresponsive human CD8^(+)TILs with a TCF1–signature and provide mechanistic insights into combining IL-15 with anti-PD-1 antibodies.

关键词： anti-PD-1 T cells TCF1 CD28 IL-15

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