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12th IFAC Symposium on Computer Applications in Biotechnology, CAB 2013

作者： Fricke, Jens Pohlmann, Kristof Vefghi, Ehsan Drews, Mats Scheffler, Ulrich Luttmann, Reiner Research Center of Bioprocess Engineering and Analytical Techniques Hamburg University of Applied Sciences Hamburg Germany

ISBN: (纸本)9783902823588

A designed multi-bioreactor plant, comprising a 5 l cell breeding BIOSTAT® Bplus system and a sixfold 11 BIOSTAT® Qplus system, was equipped with different, interlinked automation structures for systematic process controlling and reliable optimization procedures. Benefits obtained were time saving, assurance and improvement of quality and cost reduction in bioprocess production. In this report the concept for moving beyond the state-of-the-art by creating a fully automated multi-bioreactor plant is explained. The efficiency of the approach is demonstrated by investigation relating the influence of the dissolved oxygen tension pO2 in combination with the methanol concentration cS2M in Malaria vaccine production processes with Pichia pastoris. © IFAC.

关键词： Bioreactors

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Quantitative and sensitive detection of prohibited fish drugs by surface-enhanced Raman scattering

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Chinese Physics B 2018年第2期27卷 640-644页

作者：林世超张鑫赵伟臣陈朝阳杜攀赵永梅吴正龙许海军 Beijing Bioprocess Key Laboratory Beijing University of Chemical Technology College of Science Beijing University of Chemical Technology Engineering Research Center for Semiconductor Integrated Technology Institute of SemiconductorsChinese Academy of Sciences Analytical and Testing Center Beijing Normal University

Rapid and simple detections of two kinds of prohibited fish drugs, crystal violet （CV） and malachite green （MG）, were accomplished by surface-enhanced Raman scattering （SERS）. Based on the optimized Au/cicada wing, the detectable concentration of CV/MG can reach 10-7 M, and the linear logarithmic quantitative relationship curves between log/and logC allows for the determination of the unknown concentration of CV/MG solution. The detection of these two analytes in real environment was also achieved, demonstrating the application potential of SERS in the fast screening of the prohibited fish drugs, which is of great benefit for food safety and environmental monitoring.

关键词： surface-enhanced Raman scattering crystal violet malachite green quantitative detection

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Optimization of recombinant enzyme production with Pichia pastoris in an integrated industrial scale-down production plant

Optimization of recombinant enzyme production with Pichia pa...

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作者： Loegering, K. Mueller, C. Fricke, J. Bertelsen, H.-P. Scheffler, U. Luttmann, R. Research Center of Bioprocess Engineering and Analytical Techniques Hamburg University of Applied Sciences Hamburg Germany

ISBN: (纸本)9783902661708

A scale down version of a multi component production plant, including a cell breeding reactor, a protein production reactor and a disc clarifier for enzyme separation is combined to an Integrated bioprocess. Fully automated and global observable multistage parallel process courses are developed using industrial process control systems and atline measurements for enzyme concentration and enzyme activity. Optimal production conditions were found by application of DoE - Design of Experiment. © 2010 IFAC.

关键词： Enzyme activity

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Controllable design of phosphorus-doped cobalt sulfide for catalytic conversion of cellulose to methyl levulinate: The importance of Co3+/Co2+ ratio

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Renewable Energy 2025年 250卷

作者： Wu, Gang Liu, Shasha Ren, Jie Zhu, Jiawei Yu, Yun Yusup, Suzana Chen, Dengyu Zhang, Shu Huang, Yong Joint International Research Laboratory of Biomass Energy and Materials Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources College of Materials Science and Engineering Nanjing Forestry University Jiangsu Nanjing210037 China College of Intelligent Manufacturing Nanjing Polytechnic Institute Jiangsu Nanjing210044 China Department of Thermal Science and Energy Engineering National Synchrotron Radiation Laboratory University of Science and Technology of China Anhui Hefei230026 China Qingdao Institute of Bioenergy and Bioprocess Technology Chinese Academy of Sciences Shandong Qingdao266101 China Discipline of Chemical Engineering Western Australian School of Mines: Minerals Energy and Chemical Engineering Curtin University GPO Box U1987 PerthWA6845 Australia Sdn Bhd No. 1 Kawasan Institusi Penyelidikan Jalan Ayer Hitam Selangor Kajang43000 Malaysia

The development of efficient and recyclable heterogeneous catalysts for the catalytic conversion of cellulose into high-value platform chemicals has garnered significant attention. Cobalt sulfide, recognized for its earth-abundance and recyclability, has emerged as a promising catalyst for the production of methyl levulinate (ML) from cellulose, albeit with a noted disparity in catalytic performance when compared to conventional homogeneous catalysts. In this study, various phosphorus-doped cobalt sulfides (CoSxP2-x) were employed to enhance the production of ML from cellulose, with a particular focus on the sulfide-phosphorus ratio. Notably, an optimal ML yield of 48.9 wt% and a complete conversion of cellulose were achieved at a sulfide-to-phosphorus ratio of 1:1. Comprehensive characterization of the synthesized CoSxP2-x materials revealed that CoS1.00P1.00 exhibited distinctive properties, such as the presence of ternary acid sites and the lowest Co3+/Co2+ ratio among the series. Density functional theory (DFT) calculations elucidated that the bound electrons within the P-Co bonds were activated by the synergistic effects among Co, P, and S atoms, thereby diminishing the free energy barrier for the adsorption of reaction intermediates. © 2025 Elsevier Ltd

关键词： Reaction intermediates

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Modelling of ethanol production by Saccharomyces cerevisiae from a glucose and maltose mixture

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Biotechnology Letters 1995年第8期17.0卷 791-796页

作者： Lee, Yong-Seok Lee, Woo Gi Chang, Yong Keun Chang, Ho Nam BioProcess Engineering Research Center and Department of Chemical Engineering Korea Advanced Institute of Science and Technology Taejon 305-701 South Korea

A model for ethanol production from a glucose-maltose mixture has been proposed, which includes a term representing the glucose repression effect on maltose consumption. The model parameters were estimated from batch experimental data. Results of sensitivity analysis on the Monod constants for glucose and maltose, and the repression constant, showed that ±10% changes in these three parameters caused no significant effect on data fitting. © 1995 Chapman & Hall.

关键词： Zymomonas-mobilis Yeast Fermentation Mutant Strain Xylose data fitting Constants Sensitivity analysis xylose Yeast ethanol production Fermentation Mutants Maltose Catabolite Repression Zymomonas mobilis

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HSPA2 influences the differentiation and production of immunomodulatory mediators in human immortalized epidermal keratinocyte lines

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Cell Death and Disease 2025年第1期16卷 1-15页

作者： Gogler, Agnieszka Wilk, Agata Małgorzata Sojka, Damian Robert Adamiec-Organiściok, Małgorzata Matysiak, Natalia Kania, Daria Wiecha, Klaudia Małusecka, Ewa Cortez, Alexander Jorge Zamojski, Dawid Marczyk, Michał Mazurek, Agnieszka Maria Oziębło, Sylwia Scieglinska, Dorota Center for Translational Research and Molecular Biology of Cancer Maria Sklodowska-Curie National Research Institute of Oncology Gliwice Branch Wybrzeze Armii Krajowej 15 Gliwice 44-102 Poland Department of Biostatistics and Bioinformatics Maria Sklodowska-Curie National Research Institute of Oncology Gliwice Branch Wybrzeze Armii Krajowej 15 Gliwice 44-102 Poland Department of Systems Biology and Engineering Silesian University of Technology Akademicka 16 Gliwice 44-100 Poland Department of Histology and Cell Pathology Faculty of Medical Sciences in Zabrze Medical University of Silesia in Katowice Jordana 19 Zabrze 41-808 Poland Department of Data Science and Engineering Silesian University of Technology Akademicka 16 Gliwice 44-100 Poland Genetic Laboratory Gyncentrum Sp. z o.o. Sosnowiec 41-208 Poland Yale Cancer Center Yale School of Medicine New Haven CT United States

Chaperone proteins constitute a molecular machinery that controls proteostasis. HSPA2 is a heat shock-non-inducible member of the human HSPA/HSP70 family, which includes several highly homologous chaperone proteins. HSPA2 exhibits a cell type-specific expression pattern in the testis, brain, and multilayered epithelia. It is a crucial male fertility-related factor, but its role in somatic cells is poorly understood. Previously, we found that HSPA2 deficiency can impair epidermal keratinocyte differentiation. In this study, we confirmed the crucial role of HSPA2 in keratinocyte differentiation by investigating immortalized keratinocytes cultured in a reconstructed human epidermis model. Moreover, we uncovered the influence of HSPA2 on immunomodulation. Transcriptomic analysis revealed that the total loss of HSPA2 affected the expression of genes related to keratinocyte differentiation and interleukin- and interferon-mediated signaling. The functional analysis confirmed bidirectional changes associated with the loss of HSPA2. The HSPA2 knockout in HaCaT and Ker-CT keratinocytes, but not HSPA2 overproduction, impaired granular layer development as evidenced by reduced levels of late keratinocyte differentiation markers, filaggrin and involucrin, along with structural abnormalities in the upper epidermal layer. Differentiation defects were accompanied by increased mRNA expression and extracellular secretion of keratinocyte-derived pro-inflammatory IL-6 cytokine and CCL2, CCL8, CXCL1, CXCL6, and CXCL10 chemokines. The loss of HSPA2 also led to increased expression of extracellular HSPA1 and interferon-stimulated genes and secretion of immune cell modulator SLAMF7. Knocking down HSPA1 expression in keratinocytes decreased the secretion of IL-6 and CCL5 release, suggesting extracellular HSPA1’s role in the HSPA2-regulated molecular network. To summarize, we uncovered the complex homeostatic role of HSPA2 in epidermal keratinocytes. Our results suggest that dysfunction in H

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Support vectors based correlation coefficient for gene and sample selection in cancer classification

Support vectors based correlation coefficient for gene and s...

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2010 IEEE Symposium on Computational Intelligence in bioinformatics and Computational Biology, CIBCB 2010

作者： Mundra, Piyushkumar A. Rajapakse, Jagath C. BioInformatics Research Center School of Computer Engineering Nanyang Technological University Singapore Singapore

ISBN: (纸本)9781424467662

Correlation is a very widely used filter criterion for gene selection in cancer classification. However, it uses all the training samples in ranking, which may not be equally important for the classification. Using support vectors, we demonstrate that classical correlation coefficient based gene selection is biased because of the sample points away from classification margin. To remove such bias, we use only the support vectors for computation of correlation coefficient and propose a backward elimination based SVcc-RFE algorithm. The proposed method is tested on several benchmark cancer gene expression datasets and the results show improvement in classification performance compared to other state-of-the-art methods. © IEEE.

关键词： Classification (of information)

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The recovery of poly(3-hydroxybutyrate) by using dispersions of sodium hypochlorite solution and chloroform

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Biotechnology Techniques 1993年第3期7卷 209-212页

作者： Hahn, Sei Kwang Chang, Yong Keun Kim, Beom Soo Lee, Kyung Mi Chang, Ho Nam Department of Chemical Engineering and BioProcess Engineering Research Center KAIST Daeduk Science Town Taejon 305-701 South Korea

To take advantage of both differential digestion by hypochlorite and solvent extraction, we used dispersions of sodium hypochlorite solution and chloroform in the recovery of microbial PHB. The treatment with hypochlorite alone caused such severe degradation and the molecular weight decreased drastically with increasing hypochlorite concentration. However, using the dispersion, the degradation of PHB was markedly diminished owing to the shielding effect of chloroform. In this case, we could obtain PHB of above 97% purity with a number average molecular weight of 1,000,000 comparable to the original molecular weight of 1,200,000. © 1993 Kluwer Academic Publishers.

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High sensitivity technique for translation initiation site detection

High sensitivity technique for translation initiation site d...

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Proceedings of the 2004 IEEE Symposium on Computational Intelligence in bioinformatics and Computational Biology, CIBCB'04

作者： Ho, Loi Sy Rajapakse, Jagath C. Bioinformatics Research Center School of Computer Engineering Nanyang Technological University Singapore 639798

ISBN: (纸本)0780387287

Low-order Markov models are insufficient to represent hidden and complex features surrounding translation initiation sites (TISs). We present a neural network approach for detecting TISs of eukaryotes that combines lower-order models carrying biological knowledge, with non-linearity, to capture higher-order nucleotide correlations at TISs and in the surrounding coding and noncoding regions. The model consists of Markov chain models of low-order, a protein encoding model, neural networks, and a ribosome scanning model. The Markov models capture the local interaction of coding and noncoding regions while the protein encoding model utilizes evolutionary information of proteins to encode the downstream coding, sequence of the ITS. With the incorporation of ribosome scanning model, the present method allows for incorporating the biological contextual information of potential TIS into the neural network based prediction system, thereby increasing number of correct recognitions considerably. A 3-fold cross-validation evaluation on the Pedersen and Nielsen dataset yielded an average 93.8% of sensitivity and 96.9% of specificity, indicating superior accuracy of the present system to the previous highest measures of 88.5% of sensitivity and 96.3% of specificity. ©2004 IEEE.

关键词： Neural networks

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Identification of novel D11 hepatitis B surface antigen subgenotype in Jeddah, Kingdom of Saudi Arabia

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Biotechnology 2015年第3期14卷 119-128页

作者： El Hadad, Sahar Alakilli, Saleha Rabah, Samar Sabir, Jamal Department of Biological Science Faculty of Science King Abdulazi University Jeddah Saudi Arabia Research Center of Genetic Engineering and Bioinformatics VACSERA Cairo Egypt

Hepatitis B virus (HBV) infection remains to be a worldwide health problem. In Saudi Arabia, HBV is the most predominant type of hepatitis followed by hepatitis C and hepatitis A while little is known about the molecular epidemiology of the prevalence of HBV genotype/subgenotype particularly in Jeddah. Serum samples were collected from HBV chronic patients and subjected to HBsAg gene amplification. Sequencing and phylogenetic analysis of the entire HBsAg gene sequences revealed that 11 isolates belonged to HBV/D while 4 isolates were associated with HBV/C. Interestingly, HBV/D subgenotypes identified eight HBV/D present isolates belonged to HBV/D1 while three isolates showed a new cluster supporting by a branch with 99% bootstrap value and 4.3-5.8% nucleotide divergence over the entire HBsAg gene from other known subgenotypes D1 to D10, despite they were appearing more related to HBV/D5. The three strains of the new D subgenotype showed unique amino acid sequences consisting of Thr7non, 75Pro in the preS1 gene, 112Ile, 161Gly in the preS2 gene and 196Glu, 197Ala, 238Ser, 259Cys in the S gene. In addition to three amino acid residues in the S gene (373Ile, 374Ala and 381Thr) were specified S118S isolate. Subsequently, it have been verified that HBV/D1 is the most prevalent HBV subgenotype in Jeddah as well as we proposed a novel subgenotype designated HBV/D11. The identification of HBV/D11 novel subgenotypes in the present study suggested that further studies with a large number of subjects in previously examined and unexamined areas may lead to discovering new HBV strain genotypes and/or subgenotypes circulating in Saudi Arabia. © 2015 Asian Network for Scientific Information.

关键词： Amino acids

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