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High Cell Density Cultivation (HCDC) of E. coli with Intensive Multiple Sequential Batches Together with Fed-Batch at Cell Level (FBC) Superior to the Fed-Batch Technique

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Research Square 2022年

作者： Chamsart, Saethawat Malairuang, Kwanruthai Krajang, Morakot Sukna, Jatuporn Biological Science Program Faculty of Science Burapha University Chonburi 20131 Thailand Department of Biology Faculty of Science Burapha University Chonburi 20131 Thailand Biochemical Engineering Pilot Plant Burapha University Chonburi 20131 Thailand

High cell density cultivation (HCDC) is a technique that was developed and used in the cultivation of various microorganisms. Escherichia coli is the most prominent in the bacterial group and popular at the industrial scale for the production of bio-products. The HCDC conducted with the Fed-Batch modes is complicated. In this study, we developed a batch production medium (BPM), a minimal defined medium for industrial utilization with the potential use of dextrin at high concentrations as the major carbon source. Dextrin prevents cell cultivation system from the Crabtree effect due to its gradual hydrolysis to release glucose and gradual assimilation into E. coli cells like Fed-Batch at Cell Level (FBC). Yeast extract was added into the BPM to enhance cell growth. It was operated using 5-L bioreactors with the aerobic batch cultivation at high concentrations of dextrin, i.e., 90 g/L to achieve high-cell-density concentration at a high production rate superior to the Fed-Batch technique. Further, the Intensive Multiple Sequential Batch (IMFB) technique was developed to achieve a high yield at a high productivity. The maximum values of cell biomass, x;specific growth rate, μ;yield coefficient, Yx/s;productivity, rx;and efficiency (Ef) were 46.31 g/L, 0.54 h-1, 0.55 g/g, 3.86 g/L/h and 98%, respectively. The results showed that cell cultivations and growth kinetics, biochemical engineering mass balances, and design of impeller speeds using fluid dynamics in the 5-L bioreactor of aerobic batch cell cultivation for the production of E. coli cell biomass a high-density concentration using dextrin to control the FBC at a high titer can be used for the scale-up of industrial bioreactors. © 2022, CC BY.

关键词： Escherichia coli

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The Role of Fe(Iv) in the Zero-Valent Iron Biochar Activated Persulfate System for Treatment of Contaminants of Emerging Concern

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SSRN 2024年

作者： Peng, Guilong Gong, Wenwen He, Dandan Wang, Xiao Yan, Yuting Dionysiou, Dionysios D. Blaney, Lee Institute of Quality Standard and Testing Technology Beijing Academy of Agriculture and Forestry Science Beijing100097 China State Key Laboratory of Resource Insects College of Sericulture Textile and Biomass Sciences Southwest University Chongqing400715 China Environmental Engineering and Science Program Department of Chemical and Environmental Engineering University of Cincinnati CincinnatiOH45221 United States University of Maryland Baltimore County Department of Chemical Biochemical and Environmental Engineering BaltimoreMD21250 United States Yibin Academy Southwest University Yibin China

In this paper, Fe(IV) was analyzed in the zero-valent iron biochar (ZVI-BC) activated persulfate (PS) process with methyl phenyl sulfoxide (PMSO) as the probe compound. Without a reaction terminator, PMSO degradation continued during the sample holding time, resulting in significant errors in the kinetics analysis. When 1 mM NaNO2, 1 mM NH2OH, 10 mM Na2S2O3, and 10 mM dimethyl sulfoxide were used as terminators, the analytical interference to PMSO and its oxidation product, methyl phenyl sulfone (PMSO2), was reduced to a negligible level, enabling detection by high performance liquid chromatography. In addition to Fe(IV), free radical (i.e., SO4•–, •OH, O2•–) and nonradical (i.e., 1O2) reactive species were also involved in the ZVI-BC/PS system;however, Fe(IV) was the predominant reactive species. Generally, organic contaminants with electron-donating moieties rapidly reacted with Fe(IV), and the relative contribution of Fe(IV) to overall contaminant degradation decreased as the solution pH was increased. Overall, this study provided new insights into the quantitative analysis of Fe(IV) in the ZVI-BC/PS system and its application to treatment of organic contaminants in solutions with variable water quality. © 2024, The Authors. All rights reserved.

关键词： Sodium compounds

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Comparative genomics based exploration of xenobiotic degradation patterns in Glutamicibacter, Arthrobacter, and Pseudarthrobacter isolated from diverse ecological habitats

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Heliyon 2024年第22期10卷 e40280页

作者： Ghimire, Nisha Kim, Byeollee Han, So-Ra Oh, Tae-Jin Department of Life Science and Biochemical Engineering Graduate School SunMoon University Asan 31460 South Korea Bio Big Data-based Chungnam Smart Clean Research Leader Training Program SunMoon University Asan 31460 South Korea Genome-based BioIT Convergence Institute Asan 31460 South Korea Department of Pharmaceutical Engineering and Biotechnology SunMoon University Asan 31460 South Korea

Xenobiotics pose a substantial threat to environmental integrity by disrupting normal ecosystems. The genus Arthrobacter, known for its metabolic versatility can degrade several xenobiotic compounds. Arthrobacter strains have also undergone frequent taxonomic revisions and reclassifications to strains including Pseudarthrobacter and Glutamicibacter. Here, we present the complete genome sequence of Glutamicibacter protophormiae strain NG4, isolated from a coastal area surrounded by chemical plants. Further, through comparative genomics involving 55 strains from Glutamicibacter, Arthrobacter, and Pseudarthrobacter, we elucidated taxonomic relationships and xenobiotic degradation potential. Our genomics-based findings revealed a generally even distribution of xenobiotic-degrading genes and pathways among the studied strains. Glutamicibacter species emerged as potential candidate for steroid degradation. A significant number of host-specific and environmental isolates predominantly possessed pathways for 4-hydroxybenzoate (4-HB) degradation and only the environmental isolates possessed benzoate degradation pathway. Certain Arthrobacter and Pseudarthrobacter species isolated from the environmental settings were identified as potential degraders of toluene, xylene, and phenanthrene. Notably, most strains contained pathways for azathioprine, capecitabine, and 5-fluorouridine pharmaceutical drug metabolism. Overall, our findings shed light on microbial metabolic diversity among 55 strains isolated from diverse sources and hint the importance of strict environmental monitoring. Further, for the application of the putative xenobiotic degrading strains, experimental validation is required in the future. © 2024

关键词： Arthrobacter Glutamicibacter KEGG pathway Pseudarthrobacter Xenobiotic

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Beyond traditional screening: Unveiling antibiotic potentials of actinomycetes in extreme environments

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Heliyon 2024年第22期10卷 e40371页

作者： Trenozhnikova, Lyudmila P. Baimakhanova, Gul B. Baimakhanova, Baiken B. Balgimbayeva, Assya S. Daugaliyeva, Saule T. Faizulina, Elmira R. Tatarkina, Larisa G. Spankulova, Gulzhan A. Berillo, Dmitriy A. Beutler, John A. LLP Research and Production Center for Microbiology and Virology Republic of Kazakhstan Almaty Kazakhstan Department of Chemistry and Biochemical Engineering Satbayev University Republic of Kazakhstan Almaty Kazakhstan Department of Biochemistry Asfendiyarov Kazakh National Medical University Republic of Kazakhstan Almaty Kazakhstan Molecular Targets Program Center for Cancer Research National Cancer Institute National Institutes of Health Frederick MD United States

Extreme ecosystems are a rich source of specialized metabolites that can overcome multidrug resistance. However, the low efficiency of traditional exploratory research in discovering new antibiotics remains a major limitation. We hypothesized that actinomycetes may have the ability to produce antibiotics in the extremes of a changing natural environment. This study introduces a novel approach to screening natural antibiotic producers from extreme habitats based on the relationship between organisms' adaptive traits and their metabolic activities. The antibacterial and antifungal properties of 667 actinomycete isolates, obtained from 160 samples of Kazakhstan's diverse extreme habitats, were studied under neutral, saline, and alkaline conditions against MRSA, E. coli, C. albicans, and A. niger. Among these isolates, 113 exhibited antibacterial properties, and 109 demonstrated antifungal properties. Notably, one-fifth of the antagonist isolates could produce active substances solely under extreme growth conditions. Fifty-three antagonistic actinomycetes, possessing these characteristics, have been categorized into groups and warrant further investigation as potential producers of new natural antibiotics. Molecular genetic analysis of the selected isolates revealed a high prevalence of Streptomyces and Nocardiopsis strains. Furthermore, 83.4 % of obtained isolates demonstrated the ability to thrive in all studied habitats—neutral, saline, and alkaline. 96.3 % of actinomycetes isolated from extreme environments exhibited adaptation to neutral conditions, highlighting their inherent versatility. Our findings underscore the nearly complete potential (99.7 %) of isolates to overcome the salinity barrier of 3.5 % NaCl, indicating their capacity to inhabit oceanic environments. We assert that actinomycetes should be perceived as a cohesive, globally adaptive group, capable of migrating between changing conditions or remaining stable within them. These studies lay the groundw

关键词： Actinomycetes Adaptability Antibiotics Extreme habitats Novel screening approach

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Controlled release of carbamazepine at therapeutically relevant doses using photocrosslinkable polyanhydrides

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International Journal of Pharmaceutics 2025年 679卷 125728页

作者： Ghaempanah, Hamzeh Penev, Kalin I. Geraili, Armin Burneo, Jorge G Mequanint, Kibret School of Biomedical Engineering the University of Western Ontario London ON Canada Department of Chemical and Biochemical Engineering the University of Western Ontario London ON Canada Epilepsy Program Department of Clinical Neurological Sciences the University of Western Ontario London ON Canada

Epilepsy, one of the most common neurological disorders, is primarily managed with oral antiseizure medications. While effective for many patients, the short half-lives of most anti-epileptic drugs (AEDs) necessitate frequent dosing, leading to poor adherence and compromised seizure control. Controlled-release drug delivery systems offer a promising solution by maintaining consistent drug levels, reducing dosing frequency, and minimizing side effects. Among these systems, photocrosslinkable polyanhydrides are particularly promising due to their unique surface erosion properties across a wide pH range, making them ideal for oral drug delivery applications. These polymers are synthesized from cost-effective starting materials and possess tunable structures. They are also biocompatible, biodegradable, and nontoxic. This study explores the potential of photocrosslinkable polyanhydrides synthesized via thiol-ene ‘click’ chemistry for the controlled release of carbamazepine. Carbamazepine is an antiepileptic drug with poor water solubility and a narrow therapeutic range. Unlike other controlled-release systems, which faced challenges with high drug loading or compromised polymer integrity, here we demonstrate that photocrosslinkable polyanhydrides can achieve carbamazepine loadings of up to 30 wt% while preserving their surface erosion properties. Cylindrical tablets fabricated from these polymers exhibit tailored release profiles, achieving sustained drug release over 5 to 33 h by adjusting the monomer ratios. Additionally, delayed-release systems are demonstrated, allowing controlled onset times ranging from 2 to 10 h by varying the monomer composition. This approach provides a practical and efficient system for high-dose controlled-release carbamazepine delivery, offering a versatile and effective platform for enhanced epilepsy treatment. © 2025 Elsevier B.V.

关键词： Carbamazepine Controlled release tablet Epilepsy Photocrosslinkable polyanhydrides Surface erosion Therapeutic dose drug loading

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Mixing of powders inside a cyclic shear mixing device: A parametric analysis

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Journal of engineering Science and Technology Review 2019年第4期12卷 1-6页

作者： Obregón, Luis Guillermo Valencia, Guillermo Eliecer Forero, Jorge Duarte Research Group on Sustainable Chemical and Biochemical Processes Chemical Engineering Program Universidad del Atlántico Barranquilla Colombia Efficient Energy Management Research Group Mechanical Engineering Program Universidad del Atlántico Barranquilla Colombia

Although the mixture of powders in recent years has been studied with great progress, there are still a lot of parameters to consider that make it difficult to have a complete understanding. In this work, powder mixing was studied inside a simple shear mixing device consisting of a small container with two moving walls and three static walls. The aim is to obtain a basic knowledge of the internal behavior of granular materials in the surface and the height direction of the device as a function of the particle size, filling level and disturbance of the wall velocity. Results reveal that on the surface of the cavity when velocities of the moving wall are about 9 cm/s two zones are created, a high-speed zone and a low-speed zone. The width of the high-speed zone of flow is approximately 9 to 11 times the average particle size. A difference in density of 0.1 g/cm3 has no significant effect on the movement of the particles from the bottom to the top. Reasons point out to the high shear stress applied by the moving walls. © 2019 School of Science, IHU.

关键词： Particle size

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Effect of Lyophilized Peach Incorporation on Yam Cookies Quality: Oxidative Stability, Nutritional Potential and Antioxidant Properties

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SSRN 2024年

作者： Carpes, Solange Teresinha Souza da Silva, Letícia Perin, Ellen Cristina Fontoura, Bruno Henrique Pereira, Edimir Andrade de Camargo, Robson Rosa Bianchin, Mirelli de Oliveira, Jonas Cardoso Citadin, Idemir Department of Chemistry Postgraduate Program in Chemical and Biochemical Process Technology Federal Technological University of Paraná campus Pato Branco PO Box 571 PR Pato BrancoCEP 85503-390 Brazil Department of Agronomy Postgraduate Program in Agronomy Federal Technological University of Paraná campus Pato Branco PO Box 571 PR Pato BrancoCEP 85503-390 Brazil Department of Chemistry Postgraduate Program in Chemical State University of Maringá Av. Colombo 5790 - Zona 7 PR Maringá87020-900 Brazil Department of Food Engineering Federal Technological University of Paraná campus Francisco Beltrão Linha Santa Bárbara s/n PR Francisco Beltrão85601-970 Brazil

This study aimed to increase oxidative stability and enhance yam flour cookies' nutritional and antioxidant properties by incorporating lyophilized peach (LP). The cookies were subjected to two storage conditions: one with exposure to light and one in darkness for 60 days at room temperature. Four formulations were tested: a control (F1), F2 and F3 with 10% and 20% LP, respectively, and F4 with a synthetic antioxidant (SA). The cookies were a good source of protein and fiber. The incorporation of LP into yam cookies exhibited a strong antioxidative effect. Besides, the F3 shows oxidative stability comparable to the SA (F4), measured by TBARS values, under both storage conditions. However, light exposure influenced the retention of phenolics with antioxidant activity, with higher levels observed in cookies stored in darkness. The LP effectively inhibited oxidative processes, demonstrating its potential as an alternative to SA for improving the oxidative stability of yam cookies. © 2024, The Authors. All rights reserved.

关键词： Fruits

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High Potential New Industrial Species Enterobacter xiangfangenesis BUU37 for the Production of Succinic Acid by Fermentation Using Cassava Starch Hydrolysate

Research Square

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Research Square 2022年

作者： Chamsart, Saethawat Ditkunchaimongkol, Nattapoung Rattanapradit, Krongchan Sukna, Jatuporn Malairuang, Kwanruthai Krajang, Morakot Department of Biology Faculty of Science Burapha University Chon Buri20131 Thailand Biological Science Program Faculty of Science Burapha University Chon Buri20131 Thailand Biochemical Engineering Pilot Plant Faculty of Science Burapha University Chon Buri20131 Thailand Environmental Science Program Faculty of Science Burapha University Chon Buri20131 Thailand Department of Biotechnology Faculty of Science Burapha University Chon Buri20131 Thailand

New organic acid-producing bacteria were isolated from buffalo rumens. The 29 of 46 isolates could produce succinic acid (SA), but only the 6 isolates had the potential. In shake flasks culture under CO2 condition, isolate no. 37 produced SA at a concentration of 2.28 g/L, and with a better inoculum, it produced SA at 6.74 g/L with a yield coefficient, Yp/s, productivity, rp, and efficiency, Ef of 0.45 g/g, 0.28 g/L/h and 66.39%, respectively within 24 h. The 16S rRNA gene of isolate 37 was sequenced identified. It is Enterobacter xiangfangensis, later named E. xiangfangensis BUU37 which is a high potential new industrial species. Its suitable N-, C-, and carbonate sources were yeast extract, glucose hydrolysate from cassava starch hydrolysis (CSH), and MgCO3. With the medium PM II-1x of nutrients rich, CSH glucose was used efficiently at 21.46 g/L and conversed to SA at 10.80 g/L with Yp/s of 0.50 g/g, rp of 0.45 g/L/h, and Ef of 76.33%, respectively. The larger-scale productions of SA in the 5-L fermenter, with certain optimum conditions, i.e., pH 6.8, at 39 °C, 0.5 vvm CO2 sparging, agitation, and high-nutrient compositions, gave very good results. The use of PM II-2x medium of 2× nutrients and 2× C-sources of 50 g/L CSH glucose plus 25 g/L molasses and with the impeller speeds at 200-400 rpm, SA was produced at 35.52 g/L with Yp/s of 0.65 g/g, rp of 1.48 g/L/h, and Ef of 99.96%, respectively. Furthermore, with the use of PM II-4x medium with 4× the nutrients and 4× the SCH glucose at 100 g/L plus 40 g/L of molasses (a total sugar conc of 120 g/L) and with the impeller speed at 400-500 rpm, SA was produced at 53.60 g/L with Yp/s of 0.54 g/g, rp of 2.23 g/L/h, and Ef of 82.88%, respectively. The use of PM II-4.8x medium of 4.8× nutrients did not show a significant difference. For the high throughput production, the PM II-4x cultivation was modified as the repeated multiple sequential batches of 3 cycles. The results showed no significant differences of those value

关键词： Fermentation

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Improving quantitation accuracy in isobaric-labeling mass spectrometry experiments with spectral library searching and feature-based peptide-spectrum match filter

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Scientific Reports 2023年第1期13卷 1-10页

作者： Kuo, Tzu-Yun Wang, Jen-Hung Huang, Yung-Wen Sung, Ting-Yi Chen, Ching-Tai Department of Biochemical Science and Technology National Taiwan University Taipei 10617 Taiwan Bioinformatics Program Taiwan International Graduate Program Institute of Statistical Science Academia Sinica Taipei 11529 Taiwan Institute of Information Science Academia Sinica Taipei 11529 Taiwan Institute of Biomedical Informatics National Yang Ming Chiao Tung University Taipei 11221 Taiwan Department of Computer Science and Information Engineering National Taiwan University Taipei 10617 Taiwan Department of Bioinformatics and Biomedical Engineering Asia University Taichung 41354 Taiwan Center for Precision Health Research Asia University Taichung 41354 Taiwan

Isobaric labeling relative quantitation is one of the dominating proteomic quantitation technologies. Traditional quantitation pipelines for isobaric-labeled mass spectrometry data are based on sequence database searching. In this study, we present a novel quantitation pipeline that integrates sequence database searching, spectral library searching, and a feature-based peptide-spectrum-match (PSM) filter using various spectral features for filtering. The combined database and spectral library searching results in larger quantitation coverage, and the filter removes PSMs with larger quantitation errors, retaining those with higher quantitation accuracy. Quantitation results show that the proposed pipeline can improve the overall quantitation accuracy at the PSM and protein levels. To our knowledge, this is the first study that utilizes spectral library searching to improve isobaric labeling-based quantitation. For users to conveniently perform the proposed pipeline, we have implemented the feature-based filter being executable on both Windows and Linux platforms;its executable files, user manual, and sample data sets are freely available at https://***/comics/Software_*** . Furthermore, with the developed filter, the proposed pipeline is fully compatible with the Trans-Proteomic Pipeline. © 2023, Springer Nature Limited.

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A Review of the Use of Trauma Systems Therapy to Treat Refugee Children, Adolescents, and Families

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Journal of Infant, Child, and Adolescent Psychotherapy 2019年第4期18卷 417-431页

作者： Kaplin, Daniel Parente, Kristen Santacroce, Francesca A. St. Francis College United States School/Clinical Psychology program Kean University United States New York University's Biochemical engineering program United States

Children and adolescents within refugee families face adverse childhood experiences emanating from multiple sources. These traumatic events can begin with persecution within their country of origin, during the migration process, and within their re-settled countries. More specifically, these children and their families are more prone to the four core stressors being: traumatic, acculturative, isolative and resettlement stress. Trauma Systems Therapy for Refugees (TST-R) is a promising treatment model to address child and adolescent mental health disparities revolving around direct and vicarious trauma. In TST-R, the treatment team systematically targets the needs of the child, while collaborating with cultural brokers from the refugee community, their school, and family unit. Researchers have used TST-R as an intervention for reducing trauma symptoms in Somali, Somali-Bantu, and Bhutanese children, adolescents, and their families. We review the strengths and limitations of this model. Further research is needed to determine its applicability to other communities within the United States and abroad. © 2019, © 2019 JICAP Foundation, Inc.

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