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19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015

作者： Rijiravanich, P. Reboud, J. Cooper, J.M. Biochemical Engineering and Pilot Plant Research and Development Unit National Center for Genetic Engineering and Biotechnology NSTDA at KMUTT Thailand Division of Biomedical Engineering School of Engineering University of Glasgow United Kingdom

ISBN: (纸本)9780979806483

Here we demonstrate a simple and fast detection technique for one-pot immunoassay of proteins based on the color change of colloidal gold nanoparticles using surface acoustic waves (SAWs), detected with the naked eye. The simple interfacing of a piezoelectric SAW device and a disposable plastic superstrate enables a one-pot assay with no washing step, providing results in a matter of seconds and using microliter droplets. We applied the technique to the detection of human interferon-g (IFN-γ) and lysozyme, with the aggregation of gold colloids producing a visible change within 1 min. © 15CBMS-0001.

关键词： Gold nanoparticles

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Identification of plant precursor mirnas using structural robustness and secondary structures features 2017

Identification of plant precursor mirnas using structural ro...

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2017 International Conference on Biomedical engineering and Bioinformatics, ICBEB 2017

作者： Anuntakarun, Songtham Wattanapornprom, Warin Lertampaiporn, Supatcha Biological Engineering Program Faculty of Engineering King Mongkut’s University of Technology Thonburi Bangkok Thailand Research and Development Unit Thai Ocean Industries Co.Ltd Samutprakarn Thailand Biochemical Engineering and Pilot Plant Research Development Laboratory National Center for Genetic Engineering and Biotechnology Bangkok Thailand

ISBN: (纸本)9781450352970

This work presents an identification tool for plant precursor miRNAs (pre-miRNAs) using structural robustness and derivative features which can improve performance in discriminating the plant pre-miRNAs from pseudo pre-miRNAs. The classification models were trained with plant pre-miRNAs and pseudo hairpins datasets from plantMiRNAPred web site. The top 20 features were selected from four groups of features including sequence-based features, secondary structure features, base-pair features and a self-containment index score. In particular, the self-containment index score was found to be the highest informative feature among the 20 selected features. Ten-fold cross validation was applied to choose a classifier algorithm with the highest performance being among Support Vector Machine, Random Forest, Decision Tree, Naïve Bayes, K-nearest neighbor, Back-propagation Neural Network, Ripper and RBF network based on the ROC area and accuracy. The results demonstrated that the Random Forest model using 20 selected features achieved 97% accuracy and 94% sensitivity in test sets to discriminating real plant pre-miRNAs from others. © 2017 Association for Computing Machinery.

关键词： Decision trees

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Nitrogen removal of anammox cultures under different enrichment conditions

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Journal of Environmental Science and Health - Part A Toxic/Hazardous Substances and Environmental engineering 2010年第14期45卷 1832-1838页

作者： Saricheewin, Kullaya Sirivithayapakorn, Sanya Noophan, Pongsak Lek Wantawin, Chalermraj Techkarnjanaruk, Somkiet Munakata-Marr, Junko Department of Environmental Engineering Faculty of Engineering Kasetsart University Bangkok Thailand Department of Environmental Engineering Faculty of Engineering King Mongkut's University of Technology Thonburi Bangkok Thailand Biochemical Engineering and Pilot Plant Research and Development Unit National Center for Genetics Engineering and Biotechnology King Mongkut's University of Technology Thonburi Bangkhuntien Bangkok Thailand Environmental Science and Engineering Division Colorado School of Mines Golden CO United States

Anammox bacteria in sludge from an anoxic tank of a municipal wastewater reatment plant at Nongkhaem, Bangkok, Thailand were enriched in two sequencing batch reactors (SBRs;SBR-1 and SBR-2), under different conditions. SBR-1 was open to the atmosphere, while SBR-2 was closed and flushed with a mixture of 95% argon and 5% CO2 during the fill period in order to provide strict anaerobic conditions. The specific nitrogen removal rates of SBR-1 and SBR-2 were 0.43 g N/g VSS-d and 2.59 g N/g VSS-d, respectively. Denaturing gradient gel electrophoresis (DGGE) analyses showed differences in band patterns among the Nongkhaem sludge and the two enrichment cultures. Based on fluorescent in situ hybridization (FISH), the anammox bacteria in both systems were either Candidatus Brocadia anammoxidans" or Candidatus Kuenenia stuttgartiensis". The results from this study demonstrate the potential of alternative anammox systems for nitrogen removal and provide information on the microbial communi ies of anammox cultures under different enrichment conditions. Copyright © Taylor & Francis Group, LLC.

关键词： Nitrogen removal

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Mesoporous hybrid membrane based label free electrochemical immunoassay 7

Mesoporous hybrid membrane based label free electrochemical ...

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7th Biomedical engineering International Conference, BMEiCON 2014

作者： Pamsubsakul, Attasith Rijiravanich, Patsamon Surareungchai, Werasak Biological Engineering Program Faculty of Engineering King Mongkut's University of Technology Thonburi Bangkok Thailand National Center for Genetic Engineering and Biotechnology Biochemical Engineering and Pilot Plant Research and Development Unit King Mongkut's University of Technology Thounburi Bangkok Thailand School of Bioresources and Technology King Mongkut's University of Technology Thonburi Bangkok Thailand

ISBN: (纸本)9781479968015

We demonstrated an application of a mesoporous-silica hybrid membrane (MHM) toward label free electrochemical immunoassay. Mesoporous silica oriented parallel in the columnar pores of a commercial anodic alumina membrane was firstly prepared. The hybrid membrane was further functionalized with antibiodies and followed by interacting with pathogenic samples through immunorecognition event The sensing mechanism relies on the pore blocking from bound bacteria. The blockage could be traced by measuring the decrease in differential pulse voltammetric (DPV) current of ferrocenecarboxylic redox indicator. Herein, we are interested in proof of the concept on characterization of the constructed membrane, testing immunoreactivity on the membrane by ELISA and the detection of the target. The results showed the potential advantage of using this composite nanomaterial for enhancing the sensing response in the application of label free immunoassay. © 2014 IEEE.

关键词： Membranes

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Hydrothermal treatment management of high alumina waste for synthesis of nanomaterials with new morphologies

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InterCeram: International Ceramic Review 2017年第5期66卷 172-179页

作者： Abo-Almaged, H.H. Moustafa, A.F. Ismail, A.M. Amin, S.K. Abadir, M.F. Refractories Ceramic and Building Materials Department Inorganic Chemical Industries and Mineral Resources Division National Research Centre Dokki Giza Egypt Environmental Impact Assessment Unit Environmental Monitoring Laboratory Unit General Administration of Environmental Affairs Beni-Suef Governorate Egypt Industrial Technological Development Sector Ministry of Investment Trade and Industry Egypt Chemical Engineering and Pilot Plant Department Engineering Research Division National Research Centre Dokki Giza Egypt Chemical Engineering Department Faculty of Engineering Cairo University Egypt

Kiln rollers' grind waste powders (KRGW) collected from a ceramic factory are considered a high alumina waste consisting of several phases. The KRGW was treated hydrothermally at 150 °C for different time periods. The hydrothermal method was found to be a very effective method for the management of KRGW in synthesizing new nanomaterials with new morphologies. Raw and treated kiln rollers' grind waste was characterized using XRF, XRD, TEM, BET, DTA, and TGA. TEM of the raw KRGW showed a lamellar crystal structure with different shapes and morphologies. XRD displayed four different phases with a high percentage of mullite and corundum due to the high levels of Al and Si in the raw waste. Treated KRGW exhibited nano-sieves with different morphologies consisting of two predominant phases, namely calcium aluminum oxide and corundum. Results indicated that the hydrothermal process assists phase changes, crystal size, and morphology of the KRGW. This modification is expected to improve over all properties of KRGW for efficient practical applications.

关键词： Crystal structure

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Food engineering at high pressures:A numerical Study of the interaction between fluid dynamics and microbial inactivation

Food Engineering at high pressures:A numerical Study of the ...

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第十四届世界食品科技大会

作者： Panit Kitsubun Antonio Delgado Biochemical Engineering and Pilot Plant Research and Development Unit National Center for Genetic E Insitute of Fluid Mechanics Friedrich-Alexander University Erlangen-Nuremberg Cauerstraβe 4 91058

High pressure technology is being increasingly used for processing biotechnological *** pressure build-up,the work done as a result of volume change is converted into internal energy which manifests itself as temperature *** larger high pressure autoclaves,the temperature distribution is non-uniform,and this poses a major challenge in the assessment of food *** on an in-depth thermo-fluid dynamic analysis,a numerical simulation routine was developed for microbial inactivation kinetics,which was validated for high pressure treatment of various biotechnological substances at 500 MPa.

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Colorimetric Detection of Salmonella Typhimurium Using Enzyme Immobilization on Carbon Nanotubes as Biolabel

Colorimetric Detection of Salmonella Typhimurium Using Enzym...

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International Conference on Chemical, Biological, and Environmental Sciences (ICCBES’14)

作者： Porramate Chumyim Patsamon Rijiravanich Mithran Somasundrum Werasak Surareungchai School of Bioresources and Technology King Mongkut’s University of Technology Biochemical Engineering and Pilot Plant Research and Development Unit National Center for Genetic Engineering and Biotechnology National Sciences and Technology Development Agency at King Mongkut’s University of Technology

We report the colorimetric immunoassay to enhance the detection sensitivity of Salmonella enterica serovar Typhimurium by using nanocomposite as a *** platform that consists of Multiwall Carbon nanotubes(MWNTs) and enzymes layers assembled by tyrosinase(TYR) and poly-cationic polymer, poly allylamine hydrochloride(PAH).The multilayer enzyme surface of MWNTs is fabricated through a stepwise layer-by-layer electrostatic deposition *** results indicated the conjugation of MWNTs and TYR exhibited high sensitivity toward catechol substrate by producing colorimetric product *** system provides low detection limits of ***, down to 10～3 CFU/ml.

关键词： Immunosensor carbon nanotubes tyrosinase colorimetric detection S. Typhimurium

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Nitrogen assimilation in cassava: Implications for carbon metabolism and biomass synthesis 1

Nitrogen assimilation in cassava: Implications for carbon me...

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1st International Conference on Education, Science and Technology, ICON-EST 2018

作者： Siriwat, W. Muhardina, V. Thammarongtham, C. Kalapanulak, S. Saithong, T. Systems Biology and Bioinformatics Research Laboratory Pilot Plant Development and Training Institute King Mongkut's University of Technology Thonburi Bang Khun Thian Bangkok10150 Thailand Department of Agricultural Industrial Technology Faculty of Agricultural Technology University of SerambiMekkah Jl. TeungkuImumLueng Bata Batoh Banda Aceh Aceh23245 Indonesia Biochemical Engineering and Pilot Plant Research and Development Unit National Center for Genetic Engineering and Biotechnology King Mongkut's University of Technology Thonburi Bang KhunThian Bangkok10150 Thailand Bioinformatics and Systems Biology Program School of Bioresources and Technology King Mongkut's University of Technology Thonburi Bang KhunThian Bangkok10150 Thailand

The nitrogen assimilation pathway in cassava was reconstructed by comparative genomics approach to understand the underlying metabolism as well as the interaction between carbon and nitrogen assimilation towards the synthesis of metabolic phenotype. First, theproteins of cassava were annotated via sequence similarity search against genes of 11 template plants obtained from KEGG and PMN databases, employing reciprocal BLASTp(E-value ≤ 1x10-10, identity percentage ≥ 60, and coverage percentage ≥ 80). The template plants comprised well-known plant, starchy crops, nitrogen-fixing crops and crops that are evolutionarily related to cassava and includedArabidopsis thaliana, Oryzasativa, Zea mays, Ricinuscommunis, Solanumtuberosum, Brassica rapa, Cicerarietinum, Jatrophacurcas, Medicagotruncatula, Phaseolus vulgaris and Glycine *** pathway was then curatedwith reactions obtained from the CassavaCyc database to ensure full pathway *** was subsequently validated with cloned sequence of cassava from the GenBank and cassava transcriptome data from literature. The resulting N-assimilation pathway, covering the conversion of nitrate to amino acids (glutamine and glutamate),consists of 14 biochemical reactions corresponding to 59 genes, 73 proteins and 2 transport reactions. At least 92 percent of the identified proteins in the pathway were supported by the transcriptome data. In addition, the proposed N-assimilation pathway contains four additional enzymes, including glutamate synthase, nitrilase, formamidase and carbamoyl phosphate synthasecompared to the existing N-assimilation pathway in CassavaCyc database. Taken together, the N-assimilation pathway herein proposed identified reactions involved in N-assimilation and represents a forward step towards understanding metabolic basis for cassava yield as well as its phenotypic plasticity and adaptation to stress. © Published under licence by IOP Publishing Ltd.

关键词： Genes

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Detection of Salmonella enterica serovar Typhimurium in Milk Sample Using Electrochemical Immunoassay and Enzyme Amplified Labeling

Detection of Salmonella enterica serovar Typhimurium in Milk...

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International Conference on Agricultural, Environmental and Biological Sciences (AEBS-2014, Phuket)

作者： Porramate Chumyim Patsamon Rijiravanich Mithran Somasundrum Werasak Surareungchai School of Bioresources and Technology King Mongkut’s University of Technology Thonburi Biochemical Engineering and Pilot Plant Research and Development Unit National Center for Genetic Engineering and BiotechnologyNational Sciences and Technology Development Agency at King Mongkut’s University of Technology Thonburi

In this study,we present an alternative method to enhance the detection ability of Salmonella detection in milk sample by using an amplified labeling platform and electrochemical *** platform consists of multi-wall carbon nanotubes(MWNTs) and layers of the enzyme Tyrosinase(TYR),which provides an amplification cycle due to the substrate recycling *** demonstrated the immobilization of multi-layered TYR on the surface of MWNTs by using a stepwise layer-by-layer deposition ***-TYR nano-composite exhibits excellent response toward catechol in terms of high sensitivity of 320.8 m A.M-1 with short response time(2s).The labeling was applied for immunoassay detection of Salmonella in milk sample by using an easy-to-use electrochemical technique in micro-well plates coupled with disposable screen-printed electodes(SPE).The results indicate that the system provides low detection limits of Salmonella in milk sample down to <103 CFU/ml..

关键词： Carbon nanotubes Layer-by-layer Enzyme recycling system Salmonella detection

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Evaluation of CSR project from the economic, environmental and social performances monitoring by using the case study of renewable energy for a community project in Thailand 18

Evaluation of CSR project from the economic, environmental a...

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7th International Conference on Informatics, Environment, Energy and Applications, IEEA 2018

作者： Promuppatum, Napatsawan Wangnai, Chinnapong Kongpann, Apichart Boonpramote, Thitisak Chavalparit, Orathai Department of Environmental Engineering Faculty of Engineering Chulalongkorn University Bangkok10330 Thailand Pilot Plant Development and Training Institute King Mongkut's University of Technology Thonburi Bangkok10150 Thailand Energy Community Division Corporate Social Responsibility Department PTT Public Company Limited Bangkok10900 Thailand Department of Mining and Petroleum Faculty of Engineering Chulalongkorn University Bangkok10330 Thailand Research Unit of Environmental Management and Sustainable Industry Chulalongkorn University Bangkok10330 Thailand

ISBN: (纸本)9781450363624

Recently, sustainable development becomes a major global issue for the mankind. In order to meet this criteria, main objectives that composed of three aspects which are environment, economic, and social has to be completely met. Practically, the concept of corporate social responsibility (CSR) is one of a useful tools that can promote the sustainable development effectively in which decent amount of renown companies in Thailand operate and find it quite successful for some degrees. For this study, the goal was to investigate the current situation of renewable energy for a community project in Thailand. The biogas production from swine farms was used as the representative case study to evaluate the economic, environmental and social performance. The key performance indicators (KPI) were used which based on the ISO 2600:2010. The study revealed that the project provided the returns rate on the investment to the public in the form of "Social return on investment" (SROI) to be 6.59 which indicated that the CSR project is worth for the investment. In addition, the net present value (NPV) could also promote the benefits in term of economic for about 3,038,137 THB per year. For the environmental perspective, the biogas from swine farm can cause the reduction in energy/fuel consumption along with the utilization of treated waste sludge as a fertilizer which also gain the carbon credits from GHG emission mitigation. As for the social perspective, the benefit gains were in the form of monetary along with the increasing of employment rate which resulted from the CSR biogas production projects were used to calculate for the economic perspective as the direct economic value which accounted for over 389,149 THB per year. The results of this study can be a useful case study for project owners or related sectors to make the improvement and moving toward the sustainable development later on. © 2018 Association for Computing Machinery.

关键词： Sustainable development

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