Uncertainty quantification (UQ) is a vital step in using mathematical models and simulations to take decisions. The field of cardiac simulation has begun to explore and adopt UQ methods to characterise uncertainty in ...
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Reconstruction of biological and biochemical networks is a crucial step in extracting knowledge and causal information from large biological data sets. This task is particularly challenging when dealing with time-seri...
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Background: Silicone implants are biomaterials that are frequently used in the medical industry due to their physiological inertness and low toxicity. However, capsular contracture remains a concern in long-term trans...
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The human brain is composed of distinct regions that are each associated with particular functions and distinct propensities for the control of neural dynamics. However, the relation between these functions and contro...
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作者:
T C KreipkeG L NieburTissue Mechanics Laboratory
Bioengineering Graduate Program and Department of Aerospace and Mechanical Engineering University of Notre Dame Notre Dame IN USA. Tissue Mechanics Laboratory
Bioengineering Graduate Program and Department of Aerospace and Mechanical Engineering University of Notre Dame Notre Dame IN USA. gniebur@nd.edu.
147 Multidisciplinary Research Building Notre Dame IN 46556 USA. gniebur@nd.edu.
Diurnal rhythmicity in the gut maintains gut integrity, circadian rhythms, and metabolic homeostasis. However, existing studies focus on microbial composition rather than transcriptional activity. To understand microb...
Diurnal rhythmicity in the gut maintains gut integrity, circadian rhythms, and metabolic homeostasis. However, existing studies focus on microbial composition rather than transcriptional activity. To understand microbial functional dynamics, we characterize diurnal fluctuations in the mouse cecal metatranscriptome and metagenome under high-fat diet and time-restricted feeding (TRF). We show that metatranscriptomics uncovers TRF-induced time-dependent microbial functional shifts that are undetectable with metagenomics alone. We also found bile salt hydrolase ( bsh ) from Dubosiella newyorkensis exhibits diurnal expression in the TRF group. Engineering this bsh , along with other candidates, into a native E. coli chassis reveals distinct differences in deconjugation and amidation activities, underscoring functional specificity. In vivo , a D. newyorkensis bsh improves insulin sensitivity, glucose tolerance, and body composition, suggesting a direct role in TRF metabolic benefits. This study highlights how coupling metatranscriptomics with engineered bacterial systems is a powerful approach for uncovering time-dependent bacterial functions related to health and disease.
As colonoscopy is the gold standard screening test for colorectal cancer, studies of applying image processing to colonoscopy video are increasing. When performing colonoscopy or applying image processing to colonosco...
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ISBN:
(纸本)9781538670804;9788993215168
As colonoscopy is the gold standard screening test for colorectal cancer, studies of applying image processing to colonoscopy video are increasing. When performing colonoscopy or applying image processing to colonoscopy video, the presence of a residue in the large intestine is a negative affecting factor. In this reason, the colonoscopist evaluates and records the colon cleanliness after performing colonoscopy. However, these assessments can be influenced by the subjectivity of the colonoscopist. In order to quantify the frequency of residues in the large intestine, we applied image processing and machine learning techniques to the digital images obtained from colonoscopy. Support vector machine technique was applied to distinguish informative frames from colonoscopy videos, and to classify residue frames and the others. The sensitivity, specificity, and accuracy were evaluated for five patients.
N-Acyl lipids are important mediators of several biological processes including immune function and stress response. To enhance the detection of N-acyl lipids with untargeted mass spectrometry-based metabolomics, we c...
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