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Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)

作者： Jose L. Contreras-Vidal Trent J. Bradberry Harshavardhan Agashe Department of Kinesiology Graduate Programs in Bioengineering and Neuroscience & Cognitive Science University of Maryland College Park MD USA Fischell Department of Bioengineering University of Maryland College Park MD USA Graduate Program in Neuroscience & Cognitive Science University of Maryland College Park MD USA

It is generally assumed that noninvasively-acquired neural signals contain an insufficient level of information for decoding or reconstructing detailed kinematics of natural, multi-joint limb movements and hand gestures. Here, we review recent findings from our laboratory at the University of Maryland showing that noninvasive scalp electroencephalography (EEG) or magnetoencephalography (MEG) can be used to continuously decode the kinematics of 2D `center-out' drawing, unconstrained 3D `center-out' reaching and 3D finger gesturing. These findings suggest that these `far-field', extra-cranial neural signals contain rich information about the neural representation of movement at the macroscale, and thus these neural representations provide alternative methods for developing noninvasive brain-machine interfaces with wide-ranging clinical relevance and for understanding functional and pathological brain states at various stages of development and aging.

关键词： Electroencephalography Decoding Fingers Trajectory Scalp Kinematics Neurons

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The effect of contrast enhancement on familiar face recognition with simulating prosthetic vision

The effect of contrast enhancement on familiar face recognit...

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Cairo International Biomedical Engineering Conference, CIBEC

作者： M. H. Chang H. S. Kim K. S. Park Interdisciplinary Program Bioengineering Graduate School Seoul National University South Korea Department of Biomedical Engineering College of Medicine Seoul National University South Korea

Many research teams have studied to make the blind see by stimulating the retina cells using electrical devices. But due to the limited number of electrodes, it needs to complement low resolution with image processing. In this paper, we tried to find the effect of image processing, especially with contrast enhancement (CE) technique. We investigated face recognition performance with three different resolutions (8×8, 12×12, 16×16). Especially test images used here were facial images of subjects' familiar colleagues. Also we compared the performances with those of edge detection(ED) technique in 16×16 resolution. The results showed significant higher accuracy of face recognition with 8×8 resolution when using CE technique. Moreover accuracy with CE was much higher than that with ED.

关键词： Image resolution Face recognition Accuracy Prosthetics Pixel Image edge detection

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Multifunctional Nanoparticles for Targeted Chemophotothermal Treatment of Cancer Cells†

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Angewandte Chemie 2011年第33期123卷

作者： Sun‐Mi Lee Huiyul Park Joung‐Woo Choi Young Nyun Park Chae‐Ok Yun Kyung‐Hwa Yoo National Core Research Center for Nanomedical Technology Yonsei University Seoul 120‐749 (Korea) Graduate Program for Nanomedical Science Yonsei University Seoul 120‐749 (Korea) Department of Pathology Yonsei University College of Medicine Seoul 120‐749 (Korea) Department of Bioengineering Hanyang University Seoul 133‐791 (Korea) Department of Physics Yonsei University Seoul 120‐749 (Korea)

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Vapor-phase facile coatings of nanotextured organic biocompatible films on solid-state substrates

Vapor-phase facile coatings of nanotextured organic biocompa...

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作者： Goyal, Swati Kim, Young-Tae Iqbal, Samir M. Life Technologies Corporation CA 92008 United States Department of Bioengineering Nanotechnology Research and Teaching Facility University of Texas Arlington Arlington TX 76019 United States Department of Electrical Engineering Nanotechnology Research and Teaching Facility University of Texas Arlington Arlington TX 76019 United States Joint Graduate Studies Committee Bioengineering Program University of Texas United States Arlington and University of Texas Southwestern Medical Center at Dallas University of Texas Arlington Arlington TX 76019 United States

Fluorinated coatings of solid surfaces are important for many applications ranging from corrosion resistance to low surface energy biological interfaces. We present a facile approach to coat solid-state surfaces directly from vapor phase without harsh chemical or plasma treatments. The coatings show nanostructures with high surface area, which is important for variety of surface functionalizations, for example, in molecule attachment and cell growth. The novel polymeric nanoporous film is achieved from the reaction and deposition of two molecules. The surface morphology and pore sizes of the coating on solid-state surfaces can be tuned with change in concentration of monomers in a simple reaction chamber. The X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy analyses show organic nature of the coating. Cell growth studies are done to gauge biocompatibility and adsorption of proteins and cells. The approach can be used to coat, functionalize, and treat nano- and microstructures for a variety of applications with minimal chemical footprint.© 2010 IEEE.

关键词： Nanostructures

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Study of temperature effect on single-cell fluid-phase endocytosis using micro cell chips and thermoelectric devices

Study of temperature effect on single-cell fluid-phase endoc...

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14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010

作者： Lin, Ran Chang, Donald C. Lee, Yi-Kuen Bioengineering Graduate Program Hong Kong University of Science and Technology Clear Water Bay Kowloon Hong Kong Dept. of Mechanical Engineering Hong Kong University of Science and Technology Clear Water Bay Kowloon Hong Kong Dept. of Biology. Hong Kong University of Science and Technology Clear Water Bay Kowloon Hong Kong

ISBN: (纸本)9781618390622

In this work, an array-type micro cell chip with micro temperature sensors (iMTS) and a thermoelectric device was fabricated to investigate temperature effect on fluid-phase endocytosis (FPED) of HeLa cells at the single-cell level. Using two different molecular probes, large-scale single-cell FPED data (more than 500 cells) demonstrated the existence of a critical endocytosis temperature of 16-17°C for HeLa cells. Activation energy at higher temperature range (18 kcal/mol). This energy difference suggests that en-docytosis can occur through different mechanisms at different temperatures.

关键词： Temperature

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Theoretical and experimental study of electroporation of red blood cells using MEMS technology

Theoretical and experimental study of electroporation of red...

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IEEE International Conference on Nano/Micro Engineered and Molecular Systems

作者： Deng, Peigang Yin, Guangyao Zhang, Tong-Yi Chang, Donald C. Lee, Yi-Kuen KAUST-HKUST Joint Micro/Nanofluidics Lab. Hong Kong University of Science and Technology Hong Kong Hong Kong Bioengineering Graduate Program Hong Kong University of Science and Technology Hong Kong Hong Kong Dept. of Mechanical Engineering Hong Kong University of Science and Technology Hong Kong Hong Kong Dept. of Biology Hong Kong University of Science and Technology Hong Kong Hong Kong

ISBN: (纸本)9781424465439

A theoretical and experimental study of electroporation (EP) of red blood cells (RBCs) was presented in this paper. With additional strain energy, an energy-based model of an electropore induced on a RBC's membrane at different electric fields was proposed to predict the critical EP electric field strength. In addition, EP experiments with red blood cells at single-cell level was carried out on a micro EP chip. The measured critical EP electric field strengths are in agreement with the numerical predictions. ©2010 IEEE.

关键词： Strain energy

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Effect of sensor configurations on indirect-contact photoplethysmogram measurement system

Effect of sensor configurations on indirect-contact photople...

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Cairo International Biomedical Engineering Conference, CIBEC

作者： Hyun Jae Baek Soo Young Sim Jung Soo Kim Kwang Suk Park Graduate Program in Bioengineering and Advanced Biometric Research Center Seoul National University South Korea Samsung Group Interdisciplinary Program in Medical and Biological Engineering Seoul National University South Korea Department of Biomedical Engineering College of Medicine and Advanced Biometric Research Center Seoul National University South Korea

Indirect-contact photoplethysmography is a new approach to measure photoplethysmogrm over clothing for unconstrained and unconscious monitoring purpose. In this paper, the effect of sensor configurations on indirect-contact photoplehysmography was investigated. Sensor configuration was classified into two groups. One was multiple light sources with centered photo-detector configuration and the other was multiple photo-detectors with centered light source configuration. Total 8 kinds of sensors were tested. Experimental results showed that although both sensor groups have possibility to photoplethysmogram measurement over clothing, sensors with multiple light sources showed better performance when thick clothes were considered. Sensor configuration should be selected with regard to its application considerations such as power consumption or target subjects.

关键词： Light sources Light emitting diodes Photonics Biomedical measurements Cotton Clothing Thickness measurement

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Metabolomics as molecular analysis tool in cell culture engineering

Metabolomics as molecular analysis tool in cell culture engi...

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作者： Vernardis, S.I. Chrysanthopoulos, P.K. Goudar, C.T. Klapa, M.I. Foundation for Research and Technology-Hellas Metabolic Engineering and Systems Biology Laboratory Institute of Chemical Engineering and High-Temperature Chemical Processes Patras GR- 265 04 Greece Cell Culture Development Global Biological Development Bayer HealthCare 800 Dwight Way Berkeley CA 94710 United States Interdepartmental Graduate Program in Informatics in Life Sciences Medical School University of Patras Greece Departments of Chemical and Biomolecular Engineering and Bioengineering University of Maryland College Park MD 20742 United States

ISBN: (纸本)9783902661708

So far, cell culture engineering has used the high-throughput transcriptional and more recently protein profiling techniques attempting to further understand and resolve significant issues regarding the cell culture performance and its optimization. At the metabolic level, flux analysis has been used to monitor changes in the metabolic state that could affect the successful outcome of cell cultures. Metabolomics, referring to the simultaneous quantification of the (relative) concentration of the free small metabolites, is the most recently introduced high-throughput method for the measurement of the metabolic fingerprint of a biological system. Considering the role of metabolism in the context of the overall cellular function, it is easily understandable why quantifying a complete and accurate metabolomic profile is foreseen to have a major positive impact in cell culture engineering research. We will present application of Gas Chromatorgaphy-Mass Spectrometry metabolomics to monitor and analyze mammalian cell culture systems. Specifically, two bioreactor processes will be discussed in which baby hamster kidney cells were cultivated in high-cell density perfusion reactors at both laboratory and manufacturing scales and samples were analyzed over the course of the cultivation. Metabolomics enabled the differentiation between cell cultures based on bioreactor type, and stage of cell growth. Multivariate statistical analysis enabled the identification of the metabolites whose change in concentration was characteristic of the modification in any of these macroscopic parameters. The acquired results support the usefulness of metabolomics as a molecular analysis tool in cell culture engineering. © 2010 IFAC.

关键词： Bioreactors

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Hemodynamic correlates of visuomotor motor adaptation by functional Near Infrared Spectroscopy

Hemodynamic correlates of visuomotor motor adaptation by fun...

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Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)

作者： Rodolphe J. Gentili Cyrus Hadavi Hasan Ayaz Patricia A. Shewokis José L. Contreras-Vidal Department of Kinesiology and Graduate Program in Neuroscience and Cognitive Science University of Maryland College Park MD USA Department of Biology and Mathematics University of Maryland College Park MD USA School of Biomedical Engineering Science and Health Systems Drexel University Philadelphia PA USA College of Nursing and Health Professions and the School of Biomedical Engineering Science and Health Systems Drexel University Philadelphia PA USA Department of Kinesiology Fischell Department of Bioengineering and Graduate Program in Neuroscience and Cognitive Science University of Maryland College Park MD USA

The development of rehabilitation engineering technologies such as the design of smart prosthetics necessitates a deep understanding of brain mechanisms engaged in ecological situations when human interact with new tools and/or environments. Thus, we aimed to investigate potential hemodynamic signatures reflecting the level of cognitive-motor performance and/or the internal or mental states of individuals when learning a novel tool with unknown properties. These markers were derived from functional Near Infrared Spectroscopy (fNIR) signals. Our results indicate an increased level of oxy-hemoglobin in prefrontal sensors associated with enhanced kinematics during early compared with late learning. This is consistent with previous neuroimaging studies that revealed a higher contribution of prefrontal areas during early compare to late adaptation learning. These non-invasive functional hemodynamic markers may play a role in bioengineering applications such as smart neuroprosthesis and brain monitoring where adaptive behavior is important.

关键词： Hemodynamics Kinematics Electroencephalography Induction motors Educational institutions Biomedical engineering Decoding

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Wakefulness estimation only using ballistocardiogram: Nonintrusive method for sleep monitoring

Wakefulness estimation only using ballistocardiogram: Nonint...

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Annual International Conference of the IEEE Engineering in Medicine and Biology Society (EMBC)

作者： Gih Sung Chung Jeong Su Lee Su Hwan Hwang Young Kyu Lim Do-Un Jeong Kwang Suk Park Interdisciplinary Program of Bioengineering Graduate School Seoul National University South Korea Oriental Biomedical Engineering College of Health Science Sangji University South Korea Department of Neuropsychiatry and Division of Sleep Studies and Clinical Research Institute Seoul National University South Korea Department of Biomedical Engineering College of Medicine Seoul National University South Korea

To evaluate sleep quality or autonomic nervous system, many annoying electrodes have be attached to subjects' body. It can disturb comfortable sleep and, moreover, since it is very expensive experiment, continuous sleep monitoring is difficult. Since heart rate reflects the autonomic nervous system, it is highly synchronized with the sympathetic activation during transition from non-REM sleep to wakefulness. When the transition occurred the heart rate abruptly increased clearly distinguished with other changes. By using this physiology, we tried to classify the wakefulness during the whole night sleep. Our final goal is adopting this method to the continuous monitoring in our daily life. electrocardiogram (ECG) is not the suitable. Subjects have to attach the electrodes by themselves in their housing to obtain ECG. In that point of view, we used the ballistocardiogram (BCG) that is the representative method to obtain heart beat nonintrusively. For ten normal subjects, the wakefulness classifications by using the heart rate dynamics were executed. Nine subjects showed substantial agreement with the visually-scored method, polysomnography (PSG), and only one subject showed moderate agreement in Cohen's kappa value.

关键词： Sleep Heart beat Autonomic nervous system Monitoring Electrodes Electronic mail

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