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Phasor-FSTM:a new paradigm for multicolor super-resolution imaging of living cells based on fluorescence modulation and lifetime multiplexing

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Light(Science & Applications) 2025年第1期14卷 258-270页

作者： Luwei Wang Yue Chen Jiaqing Guo Xiaoyu Weng Wei Yan Jun Song Tong Ye Junle Qu Center for Biomedical Optics and Photonics&College of Physics and Optoelectronic Engineering Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong ProvinceShenzhen UniversityShenzhen518060China The Photonics Center of Shenzhen University Shenzhen UniversityShenzhen518060China Department of Bioengineering CU-MUSC Bioengineering ProgramClemson UniversityCharlestonSouth Carolina29634USA

Multicolor microscopy and super-resolution optical microscopy are two widely used techniques that greatly enhance the ability to distinguish and resolve structures in cellular *** methods have individually transformed cellular imaging by allowing detailed visualization of cellular and subcellular structures,as well as organelle ***,integrating multicolor and super-resolution microscopy into a single method remains challenging due to issues like spectral overlap,crosstalk,photobleaching,phototoxicity,and technical *** challenges arise from the conflicting requirements of using different fluorophores for multicolor labeling and fluorophores with specific properties for super-resolution *** propose a novel multicolor super-resolution imaging method called phasor-based fluorescence spatiotemporal modulation(Phasor-FSTM).This method uses time-resolved detection to acquire spatiotemporal data from encoded photons,employs phasor analysis to simultaneously separate multiple components,and applies fluorescence modulation to create super-resolution ***-FSTM enables the identification of multiple structural components with greater spatial accuracy on an enhanced laser scanning confocal microscope using a single-wavelength *** demonstrate the capabilities of Phasor-FSTM,we performed two-color to four-color super-resolution imaging at a resolution of~λ/5 and observed the interactions of organelles in live cells during continuous imaging for a duration of over 20 *** method stands out for its simplicity and adaptability,seamlessly fitting into existing laser scanning microscopes without requiring multiple laser lines for excitation,which also provides a new avenue for other super-resolution imaging technologies based on different principles to build multi-color imaging systems with the requirement of a lower budget.

关键词： resolution lifetime simplicity

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Mechanistic modeling of copper corrosions in data center environments

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Building Simulation 2024年第3期17卷 483-492页

作者： Rui Zhang Jianshun Zhang Roger Schmidt Jeremy L.Gilbert Department of Mechanical and Aerospace Engineering College of Engineering and Computer ScienceSyracuse UniversitySyracuseNY13244USA Buildings and Transportation Science Division Oak Ridge National LaboratoryOak RidgeTN37830USA SyracuseCoE-New York Center of Excellence in Environmental and Energy Systems Syracuse University727 E.Washington StreetSyracuseNY13244USA Department of Bioengineering Clemson UniversityClemson-Medical University of South Carolina Bioengineering ProgramCharlestonSC29425USA

Air-side economizers are increasingly used to take advantage of“free-cooling”in data centers with the intent of reducing the carbon footprint of ***,they can introduce outdoor pollutants to indoor environment of data centers and cause corrosion damage to the information technology *** evaluate the reliability of information technology equipment under various thermal and air-pollution conditions,a mechanistic model based on multi-ion transport and chemical reactions was *** model was used to predict Cu corrosion caused by Cl_(2)-containing pollutant *** also accounted for the effects of temperature(25℃and 28℃),relative humidity(50%,75%,and 95%),and *** also identified higher air temperature as a corrosion barrier and higher relative humidity as a corrosion accelerator,which agreed well with the experimental *** average root mean square error of the prediction was 13.7Å.The model can be used to evaluate the thermal guideline for data centers design and operation when Cl_(2)is present based on pre-established acceptable risk of corrosion in data centers’environment.

关键词： chloride pollutant mixture copper corrosion model data center temperature relative humidity synergistic effect

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Development of photoreactive demineralized bone matrix 3D printing colloidal inks for bone tissue engineering

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Regenerative Biomaterials 2023年第1期10卷 1604-1617页

作者： Katie J.Hogan Hayriye Oztatlı Marissa R.Perez Sophia Si Reyhan Umurhan Elysa Jui Ziwen Wang Emily Y.Jiang Sa R.Han Mani Diba K.Jane Grande-Allen Bora Garipcan Antonios G.Mikos Department of Bioengineering Rice UniversityMS-1426500 Main StreetHoustonTX 77030USA Baylor College of Medicine Medical Scientist Training Program HoustonTX 77030USA Institute of Biomedical Engineering Bo�gazic¸i University_Istanbul34684Turkey

Demineralized bone matrix(DBM)has been widely used clinically for dental,craniofacial and skeletal bone repair,as an osteoinductive and osteoconductive material.3D printing(3DP)enables the creation of bone tissue engineering scaffolds with complex geometries and *** methacryloylated gelatin nanoparticles(GNP-MAs)3DP inks have been developed,which display gel-like behavior for high print fidelity and are capable of post-printing photocrosslinking for control of scaffold swelling and ***,novel DBM nanoparticles(DBM-NPs,∼400 nm)were fabricated and characterized prior to incorporation in 3DP *** objectives of this study were to determine how these DBM-NPs would influence the printability of composite colloidal 3DP inks,assess the impact of ultraviolet(UV)crosslinking on 3DP scaffold swelling and degradation and evaluate the osteogenic potential of DBM-NP-containing composite colloidal *** addition of methacryloylated DBM-NPs(DBM-NP-MAs)to composite colloidal inks(100:0,95:5 and 75:25 GNP-MA:DBM-NP-MA)did not significantly impact the rheological properties associated with printability,such as viscosity and shear recovery or *** crosslinking with a UV dosage of 3 J/cm2 directly impacted the rate of 3DP scaffold swelling for all GNP-MA:DBM-NP-MA ratios with an∼40%greater increase in scaffold area and pore area in uncrosslinked versus photocrosslinked scaffolds over 21 days in phosphate-buffered saline(PBS).Likewise,degradation(hydrolytic and enzymatic)over 21 days for all DBM-NP-MA content groups was significantly decreased,∼45%less in PBS and collagenase-containing PBS,in UV-crosslinked versus uncrosslinked *** incorporation of DBM-NP-MAs into scaffolds decreased mass loss compared to GNP-MA-only scaffolds during collagenase *** in vitro osteogenic study with bone marrow-derived mesenchymal stem cells demonstrated osteoconductive properties of 3DP scaffolds for the DBM-NP-MA contents examine

关键词： demineralized bone matrix colloidal hydrogels 3D printing bone tissue engineering

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Transferrin-Mediated Nanophotosensitizer to Enhance Phototherapy with Photoacoustic Imaging Under Hypoxia

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CCS Chemistry 2025年第4期7卷 1127-1141页

作者： Mengyao Yang Yunyoung Nah Donghyeon Oh Xingshu Li Heejeong Kim Sinyoung Park Junha Lim Aibing Chen Chulhong Kim Won Jong Kim Juyoung Yoon College of Chemical and Pharmaceutical Engineering Hebei University of Science and TechnologyShijiazhuang 050018 Department of Chemistry and Nanoscience Ewha Womans UniversitySeoul 03760 Department of Chemistry POSTECH-Catholic Biomedical Engineering InstitutePOSTECHPohang 37673 Department of Electrical Engineering Convergence IT EngineeringMechanical Engineeringand Medical Science and EngineeringMedical Device Innovation CenterPOSTECHPohang 37673 Fujian Provincial Key Laboratory of Cancer Metastasis Chemoprevention and Chemotherapy College of ChemistryFuzhou UniversityFuzhou 350108 School of Interdisciplinary Bioscience and Bioengineering POSTECHPohang 37673 Graduate Program in Innovative Biomaterials Convergence Ewha Womans UniversitySeoul 03760

Various obstacles[poor tissue penetration,hypoxic tumor microenvironment,and reactive oxygen species(ROS)production quenching due to aggregation]obstruct imaging-guided high-efficiency phototherapy targeting ***,nanophthalocyanine(ZnPcN4-TF)is elaborately designed based on a synthetic phthalocyanine derivative(ZnPcN4)and nonimmunogenic transferrin(TF)through multiple noncovalent interactions for photoacoustic(PA)imaging-guided *** conjugating electron-rich amino groups effectively suppressed fluorescence and ROS generation due to the strong photoinduced electron transfer effect,ZnPcN4 became an ideal photothermal and PA imaging ***4-TF not only effectively targeted tumor sites and accumulated there,but also,surprisingly,facilitated the enhancement of ROS production via a type I process in an aggregate compared to ZnPcN4 itself,potentially due to accelerated electron ***,ZnPcN4-TF had a substantially more powerful photothermal effect than ZnPcN4 ***,this design effectively overcomes the obstacles to photodynamic therapy(aggregation-related ROS generation quenching in a hypoxic environment).Furthermore,PA imaging solves the tissue penetration challenge in optical *** study provides a broader base for designing novel photosensitizers to improve phototherapy under hypoxia.

关键词： photoacoustic imaging type I reactive oxygen species photothermal effect tumor accumulation self-assembly

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Enhancing Biomedical engineering Education Through Curricular Innovation: Developing Industry-Ready Engineers

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Biomedical engineering Education 2025年 1-7页

作者： Boyd, Lawrence Bradley, Joe Gray, Marissa Keeling, Novella M. Ochia, Ruth Reuther, Katherine E. Department of Bioengineering Clemson University Clemson USA Department of Bioengineering | Carle Illinois College of Medicine University of Illinois Urbana-Champaign Urbana USA School of Engineering Brown University Providence USA Biomedical Engineering Program University of Colorado Boulder Boulder USA Department of Bioengineering Temple University Philadelphia USA Department of Bioengineering University of Pennsylvania Philadelphia USA Center for Health Devices and Technology University of Pennsylvania Philadelphia USA

Biomedical engineering (BME) curricula are necessarily broad and it has been reported that BME students can experience substantial barriers to their career attainment compared to other engineering majors. Challenges and curricular innovations for addressing industry needs were discussed in workshops held at the BME Education Summit. This event was attended by educators and BME industry representatives. Skills needed by employers included verbal and written communication, mechanical design, engineering competency, teamwork, initiative, and self-motivation. Areas reported to be not well addressed in the BME curriculum included manufacturing, design controls, drafting, regulatory affairs, and quality systems. Specialized master’s programs are one approach to meeting industry needs. Micro-credentialing and certificate programs are another proposed innovation for appropriately training biomedical engineers for careers in industry. Another approach is via formal entrepreneurship and commercialization training. This may include having specific course offerings, assignments, and case studies focused on entrepreneurship and commercialization; having guest speakers and representatives from industry integrated in the classroom; and teaching and mentoring from faculty with expertise in these areas. Specific next steps to align academic program offerings with industry needs include directly developing specialized programs with industry partners, assessing specialty programs by both academic and industrial entities, aligning specialty program outcomes with ABET student outcomes or encouraging graduate accreditation bodies to develop a framework that directly assesses specialty programs. There is a need among the BME community to establish a broad standard for collecting informative data regarding BME-graduate industry readiness from courses, programs, employers, and recent alumni.

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Slit-Wall Differentiation Method for Depth-of-Interaction Measurement for PET

Slit-Wall Differentiation Method for Depth-of-Interaction Me...

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IEEE Symposium on Nuclear Science (NSS/MIC)

作者： D. Lee H. Hemmati M. Yi S.I. Kwon University of California Davis Biomedical Engineering Davis California United States of America Seoul National University Interdisciplinary Program in Bioengineering College of Engineering Seoul Republic of Korea

ISBN: (数字)9798350388152

ISBN: (纸本)9798350388169

In positron emission tomography (PET), accurately measuring the Depth-of-Interaction (DOI) of 511 keV gamma ray interactions is essential for improving spatial resolution in small animal PET, especially at off-center detector positions. DOI resolution can be improved by modifying crystal structures, reflectors, or by using dual readout techniques. Most methods, except for the more electronics-intensive dual side readout, increase light sharing among pixels, thereby reducing photon availability per pixel. This study introduces a novel Slit-Wall Differentiation (SWD) method, designed to restrict light sharing to just four adjacent pixels, thus enhancing DOI accuracy. The SWD method involves placing strategic slits on the adjacent faces of each pixel, controlling light sharing depending on the DOI position. DOI information is calculated from the output patterns of Silicon Photomultiplier (SiPM) arrays. The effectiveness of the proposed method was validated through GATE simulations (vGATE 9.2) using a $3 \times 3$ Lutetium-Yttrium Oxyorthosilicate (LYSO) array with enhanced specular reflectors (ESRs). An LYSO array fabricated based on the proposed configuration was successfully prepared. For practical validation, an experimental setup using a 4 $\times 4$ NUV-HD-MT (Broadcom) SiPM array is also configured.

关键词： Temperature measurement Photomultipliers Semiconductor device measurement Semiconductor detectors Position measurement Silicon Positron emission tomography

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3D bioprinting of engineered exosomes secreted from M2-polarized macrophages through immunomodulatory biomaterial promotes in vivo wound healing and angiogenesis

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Bioactive Materials 2025年第3期45卷 345-362页

作者： Sayan Deb Dutta Jeong Man An Jin Hexiu Aayushi Randhawa Keya Ganguly Tejal V.Patil Thavasyappan Thambi Jangho Kim Yong-kyu Lee Ki-Taek Lim Department of Biosystems Engineering Kangwon National University24341ChuncheonRepublic of Korea Institute of Forest Science Kangwon National University24341ChuncheonRepublic of Korea School of Medicine University of California Davis95817SacramentoUnited States Department of Bioengineering College of EngineeringHanyang University04763SeoulRepublic of Korea Department of Plastic and Traumatic Surgery Capital Medical University100069BeijingChina Interdisciplinary Program in Smart Agriculture Kangwon National University24341ChuncheonRepublic of Korea Graduate School of Biotechnology College of Life SciencesKyung Hee University17104YonginRepublic of Korea Department of Convergence Biosystems Engineering Chonnam National University61186GwangjuRepublic of Korea Department of Chemical and Biological Engineering Korea National University of Transportation27470ChungjuRepublic of Korea

Biomaterial composition and surface charge play a critical role in macrophage polarization,providing a mo-lecular cue for immunomodulation and tissue *** this study,we developed bifunctional hydrogel inks for accelerating M2 macrophage polarization and exosome(Exo)cultivation for wound healing *** this,we first fabricated polyamine-modified three-dimensional(3D)printable hydrogels consisting of algi-nate/gelatin/polydopamine nanospheres(AG/NSPs)to boost M2-exosome(M2-Exo)*** cultivated M2-Exo were finally encapsulated into a biocompatible collagen/decellularized extracellular matrix(COL@d-ECM)bioink for studying angiogenesis and in vivo wound healing *** findings show that 3D-printed AGP hydrogel promoted M2 macrophage polarization by Janus kinase/signal transducer of activation(JAK/STAT),peroxisome proliferator-activated receptor(PPAR)signaling pathways and facilitated the M2-Exo ***,the COL@d-ECM/M2-Exo was found to be biocompatible with skin ***(RNA-Seq)and real-time PCR(qRT-PCR)study revealed that co-culture of fibroblast/keratinocyte/stem cells/endothelial cells in a 3D bioprinted COL@d-ECM/M2-Exo hydrogel upregulated the skin-associated signature biomarkers through various regulatory pathways during epidermis remodeling and downregulated the mitogen-activated protein kinase(MAPK)signaling pathway after 7 *** a subcutaneous wound model,the 3D bioprinted COL@d-ECM/M2-Exo hydrogel displayed robust wound remodeling and hair follicle(HF)induction while reducing canonical pro-inflammatory activation after 14 days,presenting a viable therapeutic strategy for skin-related disorders.

关键词： Exosome Immunomodulation Decellularized extracellular matrix Skin bioprinting Angiogenesis Wound healing

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FPGA-Based Single-Input Time-to-Digital Converter with Rise Time Correction and Sub-2 ps Resolution for Precision Timing Applications

FPGA-Based Single-Input Time-to-Digital Converter with Rise ...

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IEEE Symposium on Nuclear Science (NSS/MIC)

作者： D. Lee M. Yi S.I. Kwon Biomedical Engineering University of California Davis Davis California United States of America Seoul National University Interdisciplinary Program in Bioengineering College of Engineering Seoul Republic of Korea

ISBN: (数字)9798350388152

ISBN: (纸本)9798350388169

In numerous fields, including physics, industry, and medical technology, the precise measurement of extremely short time intervals, such as those below 100 ps, is essential. This research introduces an innovative FPGA-based time-to-digital converter (TDC) implemented on the UltraScale+ platform (VCU118, Xilinx). The UltraScale+ platform improves upon timing resolution by incorporating a CARRY8 primitive cell as a delay element, an improvement over the CARRY4, which was widely used in previous platforms. Through the novel end-of-propagation (EOP) technique, the implemented TDC achieves an average bin size of 1.11 ps, demonstrating significant resource efficiency compared to other reported FPGA-based TDCs. This study details the design, implementation, and empirical validation of the TDC. Additionally, a unique feature of the proposed TDC is its ability to measure the input pulse width and apply rise time correction without requiring additional inputs. To demonstrate its practical applicability, the developed TDC was connected to the output of an ultrafast Cherenkov-Radiator-Integrated (CRI)-MCP-PMT after evaluating its electrical performance. The preliminary coincidence timing resolution of a pair of CRI-MCP-PMTs shows improvements from 107 ps to 73 ps (FWHM) with the proposed rise time correction. These advancements highlight the potential of the TDC not only for next-generation medical applications but also for its transformative impact in applications demanding ultra-precise timing.

关键词： Industries Semiconductor device measurement Microwave integrated circuits Image resolution Semiconductor detectors Pulse measurements Medical services

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Meta-Analysis of the Expansion in the Field of Structural Biology of ABC Transporters

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BioDesign Research 2022年第1期2022卷 124-135页

作者： Soomi Kim Teena Bajaj Cole Chabon Eric Tablante Tatyana Kulchinskaya Tae Seok Moon Ruchika Bajaj Stem Cell Technology Certificate Program City College of San FranciscoUSA Biotechnology Certificate Program City College of San FranciscoUSA Comparative Biochemistry Program University of California BerkeleyUSA Department of Energy Environmental and Chemical EngineeringWashington University in St.LouisUSA Department of Bioengineering and Therapeutics Sciences University of California San FranciscoUSA

ABC transporters are molecular machines which power the solute transport using ATP *** structural biology of ABC transporters has been exploding for the last few years,and this study explores timelines and trends for various attributes such as structural tools,resolution,fold,sources,and group *** study also evidences the significance of mammalian expression systems,advancements in structural biology tools,and the developing interest of group leaders across the world in the remarkably progressing *** field started in 2002 and bloomed in 2016,and COVID years were really productive to the ***,the study explores 337 structures of 58 unique ABC transporters deposited in the PDB database from which P-glycoprotein has the largest number of ***,62%of total structures are determined at the resolution of 3-4Åand 53%of structures belong to fold IV *** progressive advancements in the field,the field is shifting from prokaryotic to eukaryotic sources and X-ray crystallography to cryoelectron *** the nutshell,this study uniquely provides the detailed snapshot of the field of structural biology of ABC transporters with real-time data.

关键词： uniquely really shifting

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Reduction reactions dominate the interactions between Mg alloys and cells:Understanding the mechanisms

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Bioactive Materials 2025年第3期45卷 363-387页

作者： Jua Kim Jeremy L.Gilbert William W.Lv Ping Du Haobo Pan Shenzhen Key Laboratory of Marine Biomaterials CAS-HK Joint Lab of BiomaterialsShenzhen Institute of Advanced TechnologyChinese Academy of Sciences1068 Xueyuan AvenueShenzhen University TownShenzhen518055PR China Syracuse Biomaterials Institute Syracuse University318 Bowne HallSyracuse UniversitySyracuseNY13244USA Clemson-Medical University of South Carolina Bioengineering Program Department of BioengineeringClemson University68 Presidents StCharlestonSC39425USA Department of Orthopaedics and Traumatology Li Ka Shing Faculty of MedicineThe University of Hong KongHong Kong999077PR China Shenzhen Healthemes Biotechnology Co.Ltd Shenzhen518102PR China College of Chemical Engineering Xinjiang Normal UniversityUrumqi830054PR China

Magnesium(Mg)alloys are popular biodegradable metals studied for orthopedic and cardiovascular applications,mainly because Mg ions are essential trace elements known to promote angiogenesis and ***,Mg corrosion consists of oxidation and reduction reactions that produce by-products,such as hydrogen gas,reactive oxygen species,and *** is still unclear how all these by-products and Mg ions concomitantly alter the microenvironment and cell behaviors spatially and *** study shows that Mg corrosion can enhance cell proliferation by reducing intracellular ***,Mg cannot decrease ROS and promote cell proliferation in simulated inflammatory conditions,meaning the microenvironment is ***,cells may respond to Mg ions differently in chronic or acute alkaline pH or oxidative *** on the corrosion rate,Mg modulates HIF1αand many signaling pathways like PI3K/AKT/mTOR,mitophagy,cell cycle,and oxidative ***,this study provides a fundamental insight into the importance of reduction reactions in Mg alloys.

关键词： Magnesium alloys Electrochemical reactions Mg ions Alkaline pH Reactive oxygen species

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