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作者： Cho, Sung Hwan Chen, Chun Hao Tsai, Frank S. Godin, Jessica Lo, Yu-Hwa Materials Science and Engineering Program Jacobs School of Engineering University of California San Diego San Diego CA United States Department of Bioengineering Jacobs School of Engineering University of California San Diego San Diego CA United States Department of Electrical and Computer Engineering Jacobs School of Engineering University of California San Diego San Diego CA United States

K562 mammalian cells are sorted using a highly integrated microfabricated fluorescence-activated cell sorter (∝FACS). The sample cells are purified with an enrichment factor of 230 at a high throughput (> 1,000 ce... 详细信息

ISBN: (纸本)9781557528902

关键词： Cytology

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Fluorescence correlation spectroscopy in an optical trap

Fluorescence correlation spectroscopy in an optical trap

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作者： Hu, Y. Junio, J. Cheng, X. Ou-Yang, H.D. Physics Department Lehigh University 16 Memorial Drive East PA 18015 United States Material Science and Engineering Department Lehigh University 16 Memorial Drive East PA 18015 United States Bioengineering Program Lehigh University 16 Memorial Drive East PA 18015 United States

We have combined optical trapping and fluorescence correlation spectroscopy (FCS) to determine the trapping energy and concentration of nanoparticles in suspension by analyzing the elongated dwell time and enhanced co... 详细信息

ISBN: (纸本)9781557528902

关键词： Suspensions (fluids)

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Carbon Nanotube Wiring of Donor–Acceptor Nanograins by Self‐Assembly and Efficient Charge Transport†

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Angewandte Chemie 2011年第20期123卷

作者： Tomokazu Umeyama Noriyasu Tezuka Fumiaki Kawashima Shu Seki Yoshihiro Matano Yoshihide Nakao Tetsuya Shishido Masayuki Nishi Kazuyuki Hirao Heli Lehtivuori Nikolai V. Tkachenko Helge Lemmetyinen Hiroshi Imahori Department of Molecular Engineering Graduate School of Engineering Kyoto University Nishikyo‐ku Kyoto 615‐8510 (Japan) Fax: (+81) 75‐383‐2571 PRESTO (Japan) Science and Technology Agency 4‐1‐8 Honcho Kawaguchi Saitama 332‐0012 (Japan) Department of Applied Chemistry Graduate School of Engineering Osaka University 2‐1 Yamadaoka Suita Osaka 565‐0871 (Japan) Department of Material Chemistry Graduate School of Engineering Kyoto University Nishikyo‐ku Kyoto 615‐8510 (Japan) Department of Chemistry and Bioengineering Tampere University of Technology P.O. Box 541 33101 Tampere (Finland) Institute for Integrated Cell‐Material Sciences (iCeMS) Kyoto University Nishikyo‐ku Kyoto 615‐8510 (Japan) Fukui Institute for Fundamental Chemistry Kyoto University Sakyo‐ku Kyoto 606‐8103 (Japan)

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Mammalian cell sorting using ∞FACS

Mammalian cell sorting using ∞FACS

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Conference on Lasers and Electro-Optics (CLEO)

作者： Sung Hwan Cho Chun Hao Chen Frank S. Tsai Jessica Godin Yu-Hwa Lo Materials Science & Engineering Program Jacobs School of Engineering University of California San Diego La Jolla CA USA Department of Bioengineering Jacobs School of Engineering University of California San Diego La Jolla CA USA Department of Electrical & Computer Engineering Jacobs School of Engineering University of California San Diego La Jolla CA USA

关键词： Sorting Biomedical optical imaging Cells (biology) Optical signal processing Microfluidics Actuators Control systems Jacobian matrices Fluorescence Throughput

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Hemodynamic correlates of visuomotor motor adaptation by functional Near Infrared Spectroscopy

Hemodynamic correlates of visuomotor motor adaptation by fun...

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Annual International Conference of the IEEE engineering in Medicine and Biology Society (EMBC)

作者： Rodolphe J. Gentili Cyrus Hadavi Hasan Ayaz Patricia A. Shewokis José L. Contreras-Vidal Department of Kinesiology and Graduate Program in Neuroscience and Cognitive Science University of Maryland College Park MD USA Department of Biology and Mathematics University of Maryland College Park MD USA School of Biomedical Engineering Science and Health Systems Drexel University Philadelphia PA USA College of Nursing and Health Professions and the School of Biomedical Engineering Science and Health Systems Drexel University Philadelphia PA USA Department of Kinesiology Fischell Department of Bioengineering and Graduate Program in Neuroscience and Cognitive Science University of Maryland College Park MD USA

The development of rehabilitation engineering technologies such as the design of smart prosthetics necessitates a deep understanding of brain mechanisms engaged in ecological situations when human interact with new tools and/or environments. Thus, we aimed to investigate potential hemodynamic signatures reflecting the level of cognitive-motor performance and/or the internal or mental states of individuals when learning a novel tool with unknown properties. These markers were derived from functional Near Infrared Spectroscopy (fNIR) signals. Our results indicate an increased level of oxy-hemoglobin in prefrontal sensors associated with enhanced kinematics during early compared with late learning. This is consistent with previous neuroimaging studies that revealed a higher contribution of prefrontal areas during early compare to late adaptation learning. These non-invasive functional hemodynamic markers may play a role in bioengineering applications such as smart neuroprosthesis and brain monitoring where adaptive behavior is important.

关键词： Hemodynamics Kinematics Electroencephalography Induction motors Educational institutions Biomedical engineering Decoding

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Wakefulness estimation only using ballistocardiogram: Nonintrusive method for sleep monitoring

Wakefulness estimation only using ballistocardiogram: Nonint...

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Annual International Conference of the IEEE engineering in Medicine and Biology Society (EMBC)

作者： Gih Sung Chung Jeong Su Lee Su Hwan Hwang Young Kyu Lim Do-Un Jeong Kwang Suk Park Interdisciplinary Program of Bioengineering Graduate School Seoul National University South Korea Oriental Biomedical Engineering College of Health Science Sangji University South Korea Department of Neuropsychiatry and Division of Sleep Studies and Clinical Research Institute Seoul National University South Korea Department of Biomedical Engineering College of Medicine Seoul National University South Korea

To evaluate sleep quality or autonomic nervous system, many annoying electrodes have be attached to subjects' body. It can disturb comfortable sleep and, moreover, since it is very expensive experiment, continuous sleep monitoring is difficult. Since heart rate reflects the autonomic nervous system, it is highly synchronized with the sympathetic activation during transition from non-REM sleep to wakefulness. When the transition occurred the heart rate abruptly increased clearly distinguished with other changes. By using this physiology, we tried to classify the wakefulness during the whole night sleep. Our final goal is adopting this method to the continuous monitoring in our daily life. electrocardiogram (ECG) is not the suitable. Subjects have to attach the electrodes by themselves in their housing to obtain ECG. In that point of view, we used the ballistocardiogram (BCG) that is the representative method to obtain heart beat nonintrusively. For ten normal subjects, the wakefulness classifications by using the heart rate dynamics were executed. Nine subjects showed substantial agreement with the visually-scored method, polysomnography (PSG), and only one subject showed moderate agreement in Cohen's kappa value.

关键词： Sleep Heart beat Autonomic nervous system Monitoring Electrodes Electronic mail

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Macromol. Biosci. 2/2011

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Macromolecular Bioscience 2011年第2期11卷

作者： Tomohiro Hiraishi Eriko Masuda Daisuke Miyamoto Naoki Kanayama Hideki Abe Mizuo Maeda Bioengineering Laboratory RIKEN Advanced Science Institute 2‐1 Hirosawa Wako‐shi Saitama 351‐0198 Japan Department of Innovative and Engineered Materials Tokyo Institute of Technology 4259 Nagatsuta Midori‐ku Yokohama Kanagawa 226‐8502 Japan Present address: Asahi Kasei Medical Co. Ltd. 6‐2700 Asahi‐cho Nobeoka‐shi Miyazaki 882‐0847 Japan Present address: Graduate School of Pure and Applied Science University of Tsukuba 1‐1‐1 Ten‐noudai Tsukuba‐shi Ibaraki 305‐8573 Japan Chemical Analysis Team RIKEN Advanced Science Institute 2‐1 Hirosawa Wako‐shi Saitama 351‐0198 Japan Present address: Bioplastic Research Team Biomass Utilization Research Group RIKEN Biomass Engineering Program 2‐1 Hirosawa Wako‐shi Saitama 351‐0198 Japan

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Lee et al. reply(Note)

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Nature 2010年第7323期468卷 E4-E5页

作者： Lee, J.H. Durand, R. Gradinaru, V. Zhang, F. Goshen, I. Kim, D.-S. Fenno, L.E. Ramakrishnan, C. Deisseroth, K. a Dept. of Elec. Engineering Psychiatry and Biobehavioral Sciences Bioengineering and Radiology University of California Los Angeles CA 90095 United States b Department of Bioengineering Stanford University Stanford CA 94305 United States c Department of Electrical Engineering Korea Advanced Institute of Science and Technology (KAIST) Daejeon 305-701 South Korea d Department of Anatomy and Neurobiology Boston University School of Medicine Boston MA 02118 United States e Howard Hughes Medical Institute Stanford University Stanford CA 94305 United States f CNC Program Stanford University Stanford CA 94305 United States g Department of Psychiatry and Behavioral Sciences Stanford CA 94305 United States

Replying to N. K. Logothetis 468, 10.1038/nature09532 (2010)This is a welcome opportunity to discuss ofMRI, a technology for testing the causal and global impact of defined cell populations in vivo. The accompanying Comment reviews well-known neuroanatomy, but does seem, in its entirety, to be founded on a suggestion that, after experiments were conducted to drive a defined circuit element and measure resulting BOLD signals, we concluded that no other contributory circuit element was recruited by the driven population. This was not the case, however, as correctly understood by others in the fMRI community and as explained in the paper (for example, 'contributions from additional cells and processes downstream of the defined optically triggered population are expected and indeed represent an important aspect of this approach'). Moreover, the complexity of the brain dictates that such possible contributory mechanisms are more numerous than listed in the Comment, including many other circuit and feedback mechanisms and classes of cells within neural circuitry. As was discussed, this is one of the most important and useful aspects of the ofMRI approach. [ABSTRACT FROM AUTHOR]

关键词： Functional magnetic resonance imaging

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Reforming of jet a fuel via partial oxidation over molybdenum dioxide

Reforming of jet a fuel via partial oxidation over molybdenu...

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Fall Technical Meeting of the Western States Section of the Combustion Institute 2009, WSS/CI 2009

作者： Marin-Flores, Oscar Turba, Timothy Wang, Kang Breit, Joe Ahn, Jeongmin Norton, M. Grant Ha, Su Voiland School of Chemical Engineering and Bioengineering Washington State University P.O. Box 642710 PullmanWA99164-2710 United States School of Material Science and Mechanical Engineering Washington State University P.O. Box 642920 PullmanWA99164-2920 United States System Concept Center Boeing Commercial Airplanes 6600 Merill Creek Pkwy. EverettWA98203 United States

ISBN: (纸本)9781615676507

In the present work, the performance of molybdenum dioxide (MoO2) as catalytic material for the partial oxidation of a Jet A fuel surrogate has been investigated in terms of both catalytic activity and thermodynamic stability. Our findings indicate that the stability window of MoO2 is limited by the formation of Mo carbides under reducing environments and MoO3 in oxidizing atmospheres. However, these phase transitions do not appear to significantly affect the catalytic activity as the mechanism of reaction seems to involve only surface and probably a few subsurface layers. The catalytic activity was determined as a function of the molecular oxygen to carbon molar (O2/C) ratio, which ranged from 0.5 to 1.0. The amount of syngas produced was found to be 21-32% below the equilibrium values for hydrogen, and 3-18% for carbon monoxide. In addition, Mo dioxide was found to display higher resistance to deactivation by coking compared to that of a commercial Ni catalyst used a reference. Finally, MoO2 shows a significant tolerance to sulfur compounds such as benzothiophene, which was not able to completely deactivate the catalyst even at concentrations as high as 3000 ppmw. These properties along with the well-known electrical conductivity of MoO2 make it a promising catalytic material not only for reforming purposes but also as anode material for solid oxide fuel cells.

关键词： Catalyst activity

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Study on the feasibility of bacterial cellulose as tissue engineering scaffold

Study on the feasibility of bacterial cellulose as tissue en...

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2nd International Conference on Multi-Functional materials and Structures, MFMS-2009

作者： Ping, Wang Yi, Shi Yuanyuan, Jia Jingtong, Zheng Zongliang, Wang Yanyan, Chen Yulai, Zhou Otolaryngology-Head and Neck Surgery First Hospital Jilin University Changchun 130021 Jilin Province China Experimental Center of Bioengineering School of Pharmaceutical Sciences Jilin University Changchun 130021 Jilin Province China College of Material Science and Chemical Engineering Tianjin Science and Technology University Tianjin 300457 China State Key Laboratory of Polymer Physics and Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 China

ISBN: (纸本)0878493042

OBJECTIVE: As a kind of nanometer natural material, bacterial cellulose (BC) has been widely used in the field of food processing, paper making, pharmaceutical science, etc. In recent years, it is found that there is a good application potentiality of BC used in the medical science, especially in the field of regeneration medicine. There are some reports about BC being used in tissue engineering. It is of great necessity to investigate its tissue compatibility. In this research, the cell compatibility of BC was studied by using the method of cell-culturing combined with rat adipose- derived stem cells (ADSCs) as the seed cells. material and METHODS: BC membranes were prepared by Key Laboratory of Industrial Microbiology, Ministry of Education, Tianjin University of science and Technology. The average pore size and porosity of it were 0.8-3.5um and 93%, respectively. The ADSCs were isolated from the fat pad located in pars inguinalis of 3-weeks Wistar rats by using type II collagenase and then cultured using DMED medium contained 10% new-born calf serum. The status of cell growth was observed everyday. The cell growth curve was determined by MTT method and stem cell phenotype was identified by flow cytometry. Cells in exponential phase of growth were planted on the surface of BC membrane which had been sterilized beforehand by steam at 121°C for 20 min at cell density of 3.0x105cells/mL. The cell-BC compound was cultured by using DMED medium contained 10% new-born calf serum. The growth status and the biological characterization of ADSCs cultured on the BC were determined at different culture time. RESULTS: Most ADSCs primary cultured in vitro showed fusiform shape when they were cultured for 72 hours. The amount of the cells increased along with the culture time and confluenced at 7 days. The exponential phase of growth of primary cultured ADSCs in vitro appeared when they were cultured for 3 to 9 days. The result of flow cytometry showed that ADSCs could express th

关键词： Stem cells

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