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Bioceramic-mediated chondrocyte hypertrophy promotes calcified cartilage formation for rabbit osteochondral defect repair

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Bioactive Materials 2024年第10期40卷 306-317页

作者： Rachel H.Koh Junhee Kim Jeong-Uk Kim Seunghyun L.Kim Arun Kumar Rajendran Seunghun SLee Heesoo Lee Joo Hyun Kim Ji Hoon Jeong Yongsung Hwang Jong Woo Bae Nathaniel S.Hwang School of Chemical and Biological Engineering Institute of Chemical ProcessesSeoul National UniversitySeoul08826South Korea Interdisciplinary Program in Bioengineering Seoul National UniversitySeoul08826South Korea Department of Biomedical Engineering Dongguk UniversitySeoul10326South Korea Soonchunhyang Institute of Medi-bio Science(SIMS) Soonchunhyang UniversityCheonan31151South Korea Department of Integrated Biomedical Science Soonchunhyang UniversityAsan31538South Korea Department of Orthopaedic Surgery Konkuk University Chungju HospitalKonkuk University School of MedicineChungju27376South Korea BioMAX Institute Seoul National UniversitySeoul08826South Korea

Osteochondral tissue is a highly specialized and complex tissue composed of articular cartilage and subchondral bone that are separated by a calcified cartilage *** or gradient scaffolds,often in conjunction with stem cells and growth factors,have been developed to mimic the respective layers for osteochondral defect *** this study,we designed a hyaline cartilage-hypertrophic cartilage bilayer graft(RGD/RGDW)with ***,we demonstrated that RGD peptide-modified chondroitin sulfate cryogel(RGD group)is chondro-conductive and capable of hyaline cartilage ***,we incorporated whitlockite(WH),a Mg^(2+)-containing calcium phosphate,into RGD cryogel(RGDW group)to induce chondrocyte hypertrophy and form collagen X-rich hypertrophic *** is the first study to use WH to produce hypertrophic ***-laden RGDW cryogel exhibited significantly upregulated expression of hypertrophy markers in vitro and formed ectopic hypertrophic cartilage in vivo,which mineralized into calcified cartilage in bone ***,RGD cryogel and RGDW cryogel were combined into bilayer(RGD/RGDW group)and implanted into rabbit osteochondral defect,where RGD layer supports hyaline cartilage regeneration and bioceramic-containing RGDW layer promotes calcified cartilage *** the RGD group(monolayer)formed hyaline-like neotissue that extends into the subchondral bone,the RGD/RGDW group(bilayer)regenerated hyaline cartilage tissue confined to its respective layer and promoted osseointegration for integrative defect repair.

关键词： Bioceramic Whitlockite Bilayer scaffold Chondrocyte hypertrophy Osteochondral defect repair

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Treatment with metal–organic frameworks (MOFs) elicits secondary metabolite accumulation in Aquilaria crassna (agarwood) callus culture

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Discover Plants 2025年第1期2卷 1-9页

作者： Overmans, Sebastian Alflayyeh, Yazan Gutiérrez, Sergio Aldlaigan, Yousef Lauersen, Kyle J. Bioengineering Program Biological and Environmental Sciences and Engineering Division King Abdullah University of Science and Technology (KAUST) Jeddah Kingdom of Saudi Arabia Plant Tissue Culture & Biotechnology Center Ministry of Environment Water and Agriculture of Saudi Arabia (MEWA) Riyadh Kingdom of Saudi Arabia

Thymelaceaous trees are prized for accumulating fragrant resins composed of hundreds of secondary metabolites in their woody tissues. Slow growth and increasing consumer demand have stretched natural sources of agarwood trees to being endangered and alternative production modes, including silviculture and tissue culture, are currently being investigated. Dedifferentiated tissue culture of agarwood trees provides a means of cell propagation independent of environmental context. However, secondary metabolite accumulation, as found in fragrant resins, occurs largely in response to wounding. Here, we investigated the application of metal-organic frameworks (MOFs) as potential elicitors of secondary metabolite formation in Aquilaria crassna tissue culture. Callus cultures were exposed to five commercially available MOFs (UiO-67, MOF-808, HKUST-1, ZIF-67, and MOF-74) and ethanol extracts were analysed using GC-MS to quantify secondary metabolite accumulation in MOF-exposed cultures compared to untreated cultures. Samples that were exposed to either of the two Zr-based MOFs exhibited metabolite production profiles that were similar to each other, (trans-2-Carboxy-cyclo-hexyl)-acetic acid was reduced in the presence of all MOFs, the Cu-containing HKUST-1 MOF increased palmitic acid levels, and ZIF-67 (at 20 mg/L) was found to elicit the highest accumulation of secondary metabolites with potential fragrance applications. These results demonstrate the possibility of eliciting secondary metabolites from dedifferentiated agarwood tree cell culture and may provide an alternative means of sourcing fragrant specialty chemicals from these plants.

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Materials and devices for flexible and stretchable photodetectors and light-emitting diodes

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Nano Research 2021年第9期14卷 2919-2937页

作者： Jun-Kyul Song Min Sung Kim Seungwon Yoo Ja Hoon Koo Dae-Hyeong Kim Center for Nanoparticle Research Institute for Basic Science(IBS)Seoul 08826Republic of Korea School of Chemical and Biological Engineering and Institute of Chemical Processes Seoul National UniversitySeoul 08826Republic of Korea Interdisciplinary Program for Bioengineering Seoul National UniversitySeoul 08826Republic of Korea Department of Materials Science and Engineering Seoul National UniversitySeoul 08826Republic of Korea

Recently,significant efforts have been directed at overcoming the limitations of conventional rigid optoelectronic devices,particularly their poor mechanical stability under bending,folding,and stretching *** of major approaches for rendering optoelectronic devices mechanically deformable is to replace the conventional electronic/optoelectronic materials with functional nanomaterials or organic materials that are intrinsically flexible/***,advanced device designs and unconventional fabrication methods have also contributed to the development of soft optoelectronic ***,new devices such as bio-inspired curved image sensors,wearable light emitting devices,and deformable bio-integrated optoelectronic devices have been *** this review,recent progress in the development of soft optoelectronic materials and devices is ***,various materials such as nanomaterials,organic materials,and their hybrids that are suitable for developing deformable photodetectors,are ***,the nanomaterials and organic/polymeric materials that are applicable in deformable light-emitting diodes are ***,representative system-level applications of flexible and stretchable photodetectors and light-emitting diodes are reviewed,and future prospects are discussed.

关键词： nanomaterial organic material photodetector light-emitting diode flexible optoelectronics stretchable optoelectronics

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Novel single-cell mtDNA sequencing technology reveals hidden mutations in oocytes, blastoids, and stem cells

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Life Medicine 2023年第4期2卷 1页

作者： Ismail M. Shakir Mo Li Bioscience Program Biological and Environmental Science and Engineering Division King Abdullah University of Science and Technology Thuwal 23955-6900 Kingdom of Saudi Arabia Bioscience Program Biological and Environmental Science and Engineering Division King Abdullah University of Science and Technology Thuwal 23955-6900 Kingdom of Saudi Arabia Bioengineering Program Biological and Environmental Science and Engineering Division King Abdullah University of Science and Technology Thuwal 23955-6900 Kingdom of Saudi Arabia

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DROPLET DIGITAL IMMUNO-PCR FOR PROFILING PROTEIN-SPECIFIC EXTRACELLULAR VESICLES 26

DROPLET DIGITAL IMMUNO-PCR FOR PROFILING PROTEIN-SPECIFIC EX...

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26th International Conference on Miniaturized Systems for Chemistry and Life sciences, MicroTAS 2022

作者： Lix, Chxnchen Lin, Hxixian Yao, Shxhxai Department of Laboratory Medicine Nanfang Hospital Southern Medical University Guangzhou China Department of Mechanical and Aerospace Engineering The Hong Kong University of Science and Technology Clear Water Bay Kowloon Hong Kong Bioengineering Graduate Program Department of Chemical and Biological Engineering The Hong Kong University of Science and Technology Clear Water Bay Kowloon Hong Kong

ISBN: (纸本)9781733419048

We construct a versatile droplet digital immuno-PCR ddiPCR assay which integrates immuno-PCR and droplet digital PCR ddPCR for quantifying protein-specific extracellular vesicle EV subpopulations at the single-vesicle level. We applied the ddiPCR assays for profiling the EV proteins of CD/CD63/CD81, HER2, EpCAM in a breast cancer cohort. The results demonstrated that the counting of multi-subpopulation EVs could significantly distinguish patients with breast tumor at different stages or hepatocellular carcinoma from healthy controls. This study provides a promising strategy to count multi-subpopulation EVs at the single-vesicle level for cancer diagnosis. © 2022 MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life sciences. All rights reserved.

关键词： Drops

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3D bioprinting of engineered exosomes secreted from M2-polarized macrophages through immunomodulatory biomaterial promotes in vivo wound healing and angiogenesis

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Bioactive Materials 2025年第3期45卷 345-362页

作者： Sayan Deb Dutta Jeong Man An Jin Hexiu Aayushi Randhawa Keya Ganguly Tejal V.Patil Thavasyappan Thambi Jangho Kim Yong-kyu Lee Ki-Taek Lim Department of Biosystems Engineering Kangwon National University24341ChuncheonRepublic of Korea Institute of Forest Science Kangwon National University24341ChuncheonRepublic of Korea School of Medicine University of California Davis95817SacramentoUnited States Department of Bioengineering College of EngineeringHanyang University04763SeoulRepublic of Korea Department of Plastic and Traumatic Surgery Capital Medical University100069BeijingChina Interdisciplinary Program in Smart Agriculture Kangwon National University24341ChuncheonRepublic of Korea Graduate School of Biotechnology College of Life SciencesKyung Hee University17104YonginRepublic of Korea Department of Convergence Biosystems Engineering Chonnam National University61186GwangjuRepublic of Korea Department of Chemical and Biological Engineering Korea National University of Transportation27470ChungjuRepublic of Korea

Biomaterial composition and surface charge play a critical role in macrophage polarization,providing a mo-lecular cue for immunomodulation and tissue *** this study,we developed bifunctional hydrogel inks for accelerating M2 macrophage polarization and exosome(Exo)cultivation for wound healing *** this,we first fabricated polyamine-modified three-dimensional(3D)printable hydrogels consisting of algi-nate/gelatin/polydopamine nanospheres(AG/NSPs)to boost M2-exosome(M2-Exo)*** cultivated M2-Exo were finally encapsulated into a biocompatible collagen/decellularized extracellular matrix(COL@d-ECM)bioink for studying angiogenesis and in vivo wound healing *** findings show that 3D-printed AGP hydrogel promoted M2 macrophage polarization by Janus kinase/signal transducer of activation(JAK/STAT),peroxisome proliferator-activated receptor(PPAR)signaling pathways and facilitated the M2-Exo ***,the COL@d-ECM/M2-Exo was found to be biocompatible with skin ***(RNA-Seq)and real-time PCR(qRT-PCR)study revealed that co-culture of fibroblast/keratinocyte/stem cells/endothelial cells in a 3D bioprinted COL@d-ECM/M2-Exo hydrogel upregulated the skin-associated signature biomarkers through various regulatory pathways during epidermis remodeling and downregulated the mitogen-activated protein kinase(MAPK)signaling pathway after 7 *** a subcutaneous wound model,the 3D bioprinted COL@d-ECM/M2-Exo hydrogel displayed robust wound remodeling and hair follicle(HF)induction while reducing canonical pro-inflammatory activation after 14 days,presenting a viable therapeutic strategy for skin-related disorders.

关键词： Exosome Immunomodulation Decellularized extracellular matrix Skin bioprinting Angiogenesis Wound healing

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Update on synthetic biomaterials combined with fibrin derivatives for regenerative medicine:Applications in bone defect treatment:Systematic review

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World Journal of Orthopedics 2025年第5期16卷 93-106页

作者： Bruna Trazzi Pagani Marcelie Priscila de Oliveira Rosso Matheus Bento Medeiros Moscatel Beatriz Flavia de Moraes Trazzi Marcelo Rodrigues da Cunha João Paulo Mardegan Issa Daniela Vieira Buchaim Rogério Leone Buchaim Dentistry School University of MariliaMarilia 17525-902Brazil Department of Physiotherapy Physical Education and BiomedicineEstácio de SáSchool of OurinhosOurinhos 19907-510Brazil Interunit Graduate Program in Bioengineering University of Sao PauloSao Carlos 13566-970Brazil Postgraduate Program in Health Sciences Faculty of Medicine of JundiaíJundiai 13202-550Brazil Department of Basic and Oral Biology Ribeirão Preto School of DentistryUniversity of São PauloRibeirao Preto 14040-904Brazil Medical School University Center of AdamantinaAdamantina 17800-000Brazil Graduate Program in Anatomy of Domestic and Wild Animals Faculty of Veterinary Medicine and Animal ScienceUniversity of Sao PauloSao Paulo 05508-270Brazil Department of Biological Sciences Bauru School of DentistryUniversity of São PauloBauru 17012-901Brazil

BACKGROUND Bone regeneration is a central focus of regenerative medicine,with applications in orthopedics and dentistry,particularly for treating bone defects caused by trauma,infection,or congenital *** biomaterials,often combined with fibrin derivatives,offer promising solutions for bone healing and *** To Explore the increasingly important role of the association of synthetic biomaterials with fibrin in bone *** Search terms included:“synthetic biomaterials AND fibrin sealant”,“hydroxyapatite AND fibrin sealant”,“tricalcium phosphate AND fibrin sealant”,and“synthetic biomaterials AND platelet-rich fibrin(PRF)”,resulting in 67 *** rigorous screening,21 articles met the inclusion *** The reviewed studies assessed biomaterials like hydroxyapatite(HA),β-tricalcium phosphate(β-TCP),and fibrin-based *** findings highlighted the enhanced osteoconductivity and biocompatibility of HA andβ-TCP,especially when combined with fibrin *** composites show significant potential for improving cellular adhesion,promoting osteogenic differentiation,and accelerating bone *** antimicrobial properties and structural support for cell growth of certain biomaterials indicate a promising potential for clinical *** This systematic review emphasizes the growing role of fibrin-based biomaterials in bone regeneration and urges continued research to improve their clinical use for complex bone defects.

关键词： Biomaterials Bone regeneration Fibrin sealant Hydroxyapatite Orthopedics Dentistry Regenerative medicine

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Droplet digital quantification of nucleic acids with Cas13a 25

Droplet digital quantification of nucleic acids with Cas13a

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25th International Conference on Miniaturized Systems for Chemistry and Life sciences, MicroTAS 2021

作者： Liu, Frank X. Cui, Johnson Q. Yao, Shuhuai Department of Mechanical and Aerospace Engineering The Hong Kong University of Science and Technology Clear Water Bay Kowloon Hong Kong Bioengineering Graduate Program Department of Chemical and Biological Engineering The Hong Kong University of Science and Technology Clear Water Bay Kowloon Hong Kong

ISBN: (纸本)9781733419031

The isothermal amplification coupled with CRISPR detection has attracted tremendous interest for the sensitive, specific, and qualitative detection of nucleic acids. We report here a Microfluidics Enabled Digital Isothermal CRISPR-cas13a Assay (MEDICA) which leverages the droplet microfluidics technology to enable recombinase polymerase amplification (RPA) and Cas13a trans-cleavage reporting in individual droplet partitions for absolute quantification. The optimal one-pot MEDICA achieved single nucleic acid molecule detection within 30 min. We applied MEDICA to quantify HPV 16 and 18 viral loads in clinical samples, indicating complete accordance with quantitative polymerase chain reaction (qPCR) results. © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life sciences. All rights reserved.

关键词： Drops

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Cover Feature: Compartmentalized Sesquiterpenoid Biosynthesis and Functionalization in theChlamydomonas reinhardtiiPlastid (ChemBioChem 5/2025)

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ChemBioChem 2025年第5期26卷

作者： Sergio Gutiérrez Sebastian Overmans Gordon B. Wellman Kyle J. Lauersen Bioengineering Program Biological Environmental Sciences and Engineering Division (BESE) King Abdullah University of Science and Technology (KAUST) Thuwal Saudi Arabia

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LoraMap: Harnessing the Power of LoRA Connections

arXiv

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arXiv 2024年

作者： Park, Hyeryun Kwak, Jeongwon Jang, Dongsuk Park, Sumin Choi, Jinwook Interdisciplinary Program for Bioengineering Graduate School Seoul National University Korea Republic of Integrated Major in Innovative Medical Science Graduate School Seoul National University Korea Republic of Department of Biomedical Engineering College of Medicine Seoul National University Korea Republic of Medical Research Center Institute of Medical and Biological Engineering Seoul National University Korea Republic of

Fact-checking techniques can mitigate hallucinations in Large Language Models (LLMs), a prominent issue in specialized domains. As parameter-efficient techniques such as Low-Rank Adaptation (LoRA) can overcome substantial computational overhead, some studies have explored the integration of multiple LoRAs. While previous studies focus on parallel integration, this paper investigates methods to establish connections among multiple LoRAs. We create three reasoning datasets tailored to fact-checking and fine-tune individual LoRAs, allowing them to view and reason from diverse perspectives. Then, we explore strategies for allocating these reasoning LoRAs and introduce LoraMap, an approach to map connections between them. The results of the fact-checking task demonstrate that the performance of LoraMap is superior to LoraHub, an existing method for integrating LoRAs. LoraMap also outperforms with significantly fewer trainable parameters than LoraConcat, which concatenates LoRAs and further fine-tunes them. Copyright © 2024, The Authors. All rights reserved.

关键词： Problem oriented languages

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