Gas vesicles (GVs), nanostructure gas-filled protein-shelled compartments, act as ultrasound contrast agents (UCAs). Recent works report bioengineered GvpC, one of the shell proteins, potentializes interactions with s...
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Purpose: Foot and ankle pathologies are among the most prevalent conditions within the human locomotor system. Imaging examinations are crucial for diagnosing, treating, and achieving satisfactory functional outcomes ...
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Colorectal cancer (CRC) ranks among the most prevalent tumors, particularly affecting men and women, with mouse models and imaging technologies like colonoscopy, endoluminal (eUBM), and transabdominal (tUBM) ultrasoun...
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ISBN:
(数字)9798350349085
ISBN:
(纸本)9798350349092
Colorectal cancer (CRC) ranks among the most prevalent tumors, particularly affecting men and women, with mouse models and imaging technologies like colonoscopy, endoluminal (eUBM), and transabdominal (tUBM) ultrasound biomicroscopy playing pivotal roles in exploring new treatment avenues. The evaluation of colon wall thickness (Wt), a significant marker of tumor presence, is feasible through eUBM or tUBM, contrasting with colonoscopy's limitations. This study scrutinizes the consistency of Wt measurements obtained via eUBM and tUBM in mice, with or without colon tumors induced by azoxymethane (AOM) and dextran sulfate sodium salt (DSS). UBM images were captured using both eUBM and tUBM instruments, with subsequent Wt measurements performed using ImageJ software. Comparison between Wt obtained from eUBM and tUBM images used the One-Way ANOVA, for normal distributed data, or the Kruskal-Wallis test otherwise. Local Ethics Committee for Animal Use in Research approved the work (protocol 014/20). In general, the results indicate a substantial median Wt disparity between control and tumor groups measured by both techniques (p<0.05). Notably, discrepancies in Wt findings between the two methods were observed in the control group (p<0.05), despite the excellent resolution of the images in delineating colon layers.
Gas vesicles (GVs), gas-filled protein-encapsulated nanostructures, synthesized by microorganisms such as Halobacterium salinarum NRC-1 (HALO) and Anabaena flos aquae (ANA) have been reported as potential ultrasound c...
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ISBN:
(数字)9798350349085
ISBN:
(纸本)9798350349092
Gas vesicles (GVs), gas-filled protein-encapsulated nanostructures, synthesized by microorganisms such as Halobacterium salinarum NRC-1 (HALO) and Anabaena flos aquae (ANA) have been reported as potential ultrasound contrast agents (UCA) due to their ability to mechanically vibrate with the same frequency, or harmonics, as the one from the incident ultrasound (US) wave. Despite GVs resonating in frequencies above 21 MHz, they collapse irreversibly when the US incident field pressure exceeds the vesicle collapse pressure. There are few reports on the relationship between the pressure of the acoustic wave incident in the GVs and the corresponding intensity of the backscattered wave signal from the GVs and, therefore, this study was designed to investigate how varying the relative incident US power, at 21MHz, reflects on the intensity of the US backscattered signal and the collapse of HALO and ANA GVs. The mean (±SD) of the grey level intensity for the ROIs, in maximum intensity pixel images, with GVs and PBS, were determined for transmitted acoustic relative power of 1, 5, 10, 20%. ANA-GVs presented a lower signal intensity, but greater resistance to the increase in relative US pressure. In conclusion, the best behavior was for the Halo-GVs at 5% power and at 21MHz frequency.
This article proposes the creation, implementation and development of an integration plugin for ImageJ that makes calls in MATLAB functions for the segmentation UBM imagens, more specific endoluminal (eUBM) e transabd...
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ISBN:
(数字)9798350349085
ISBN:
(纸本)9798350349092
This article proposes the creation, implementation and development of an integration plugin for ImageJ that makes calls in MATLAB functions for the segmentation UBM imagens, more specific endoluminal (eUBM) e transabdominal (tUBM), in the laboratory the images are generating with frequencies between 30-40 MHZ. These images allow view colon layer, identify tumors in colons, to calculate tumor volume and 3D visualization. This integration may to improve the time taken to perform the procedure and the user experience to reduce the number of tasks and change windows, making the experience more fluid and with less risk of errors.
Gas vesicles (GVs), nanostructure gas-filled protein-shelled compartments, act as ultrasound contrast agents (UCAs). Recent works report bioengineered GvpC, one of the shell proteins, potentializes interactions with s...
Gas vesicles (GVs), nanostructure gas-filled protein-shelled compartments, act as ultrasound contrast agents (UCAs). Recent works report bioengineered GvpC, one of the shell proteins, potentializes interactions with specific molecules in tumor microenvironment. An important target of investigation is Cathepsin B (CTSB), an enzyme produced by several tumors. CTSB acts in the tumoral microenvironment to increase tumor growth and invasion. This work objective relied on bioengineering the GVs to promote the GvpC-CTSB interaction with a subsequent increase of ultrasound (US) signal backscattered. GvpC was engineered to contain a known CTSB cleavage sequence re-added in striped GV (ΔGV). Western Blot (WB) test was used to compare the GVwt (wild type) and GV* (GV engendered) before and after 24 hours reaction with CTSB. US images for PBS (negative control), GVtw, ΔGV, GV* and CTSB+GV*, were obtained in 21MHz (5% total power) and regions-of-interest (ROIs) over the final B-mode image were determined. The WB test for GVwt resulted in mean (±SD) GvpC concentrations of 25169 (±8574) and 32873 (±9113) and GV* was 32487 (±6867) and 12763 (±2846), at t0 and t24, respectively. The US image gray level intensities for the ROIs related to the samples of PBS, GVwt, ΔGV, GV* and CTSB+GV* were 55.8 (±17.6), 47.4 (±16.6), 46.1 (±22.8) and 150.2 (±24), respectively. These results reveal an increase in the US signal backscattered from GV* after CTSV reaction. This increase is likely related to a direct GvpC degradation by CTSB, without affecting the GV* structure. In conclusion, high frequency US B-mode images showed the GV* with the potential to act as an UCA sensitive to the CTSB presence in tumoral microenvironment.
Purpose: Ultrasound contrast agent (UCA), used to enhance the contrast in ultrasonography, consists of microbubbles filled with an inert gas and encapsulated with a protein or a lipidic-stabilize membrane. The UCAs ar...
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Colon cancer has a high incidence, without considering skin cancer, being the third most common cancer in the world. Despite this high incidence and high mortality rate, most of the outcomes could be prevented by usin...
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ISBN:
(数字)9781728154480
ISBN:
(纸本)9781728154497
Colon cancer has a high incidence, without considering skin cancer, being the third most common cancer in the world. Despite this high incidence and high mortality rate, most of the outcomes could be prevented by using accurate techniques for early cancer detection to enable early treatment. In this context, it is important to conduct preclinical studies, using animal models of colon diseases, to understand the development of the disease and to test new diagnostic approaches including imaging instrumentation. We have previously used a 3-French ultrasound mini-probe to image the layered structure in the mouse colon, to detect polyps, and to visualize their invasion through the colon wall. The present work aimed to measure the colon wall thickness and perform a numeric differentiation between the thickness of normal and inflamed mouse colon, a crucial step to elucidate the disease progression in colorectal cancer mouse model.
Blood clotting, a process to avoid bleeding, involves vasoconstriction, platelet plug build-up, coagulation, clot retraction and fibrinolysis. During coagulation, blood changes from liquid to gel, and the clot viscoel...
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During coagulation, blood changes from liquid to gel, and the clot viscoelastic properties continue modifying until fibrinolysis. Ultrasound (US) was used in this work to trace the time-changing shear modulus, μ, dur...
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