Microscale electroporation devices are mostly restricted to in vitro experiments(i.e.,microchannel and microcapillary).Novel fiber-based microprobes enable in vivo microscale electroporation and arbitrarily select the...
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Microscale electroporation devices are mostly restricted to in vitro experiments(i.e.,microchannel and microcapillary).Novel fiber-based microprobes enable in vivo microscale electroporation and arbitrarily select the cell groups of interest to *** developed a flexible,fiber-based microscale electroporation device through a thermal drawing process and femtosecond laser micromachining *** fiber consists of four copper electrodes(80μm),one microfluidic channel(30μm),and has an overall diameter of 400μ*** dimensions of the exposed electrodes and channel were customizable through a delicate femtosecond laser *** feasibility of the fiber probe was validated through numerical simulations and in vitro *** reversible and irreversible microscale electroporation was observed in a 3D collagen scaffold(seeded with U251 human glioma cells)using fluorescent *** ablation regions were estimated by performing the covariance error ellipse method and compared with the numerical *** computational and experimental results of the working fiber-based microprobe suggest the feasibility of in vivo microscale electroporation in space-sensitive areas,such as the deep brain.
In this research, LiDAR sensor technology introduces a new representation of point cloud data for tasks in 3D object recognition. Point clouds provide rich information that can be utilized to predict the orientation p...
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We report the application of widefield birefringence microscopy for 3D rendering of sparse myelinated fibers in human brain gray matter. This inexpensive and simple method offers the potential to inform studies of the...
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We demonstrate mode-division multiplexing at visible wavelengths (473 nm) for the first time using adiabatic mode couplers. We measure less than -15 dB and -20 dB crosstalk for TE2 and TE3 higher-order mode couplers, ...
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We demonstrate mode-division multiplexing at visible wavelengths (473 nm) for the first time using adiabatic mode couplers. We measure less than -15 dB and -20 dB crosstalk for TE2 and TE3 higher-order mode couplers, ...
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Fluorescence Lifetime Imaging (FLIM) is a powerful technique that measures the decay time of fluorophores present in tissue samples alluding to their constituent molecules. FLIM has gained popularity in biomedical ima...
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ISBN:
(纸本)9781510669659
Fluorescence Lifetime Imaging (FLIM) is a powerful technique that measures the decay time of fluorophores present in tissue samples alluding to their constituent molecules. FLIM has gained popularity in biomedical imaging for applications such as detecting cancerous tumors, surgical guidance, etc. However, conventional FLIM systems are limited by a reduced number of spectral bands and long acquisition time. Moreover, the large footprint, complexity, and cost of the instrumentation make it difficult for clinical applications. In this paper, we demonstrate a reconstruction-based hyperspectral detector that can resolve decay time and intensities in broad spectral ranges while providing high sensitivity, high gain, and fast response time. The hyperspectral detector is comprised of an array of efficient, ultrafast avalanche photodetectors integrated with nanophotonic structures. We utilize different nanostructures in the detectors to modulate light-matter interactions in spectral channels. This allows us to computationally reconstruct the spectral profile of the incoming fluorescence spectrum without the need for additional filters or dispersive optics. Also, the nanophotonic structures enhance efficiency (by a factor of 2 to 10 over different wavelengths) while providing fast response time. An innovative detector design has been employed to reduce the breakdown of the avalanche photodetectors to-7.8V while maintaining high gain (∼50) across the spectral range. Therefore, enabling low light detection with a high signal-To-noise ratio for FLIM applications. Added spectral channels would provide valuable information about tissue materials, morphology, and disease diagnosis. Such innovative hyperspectral sensors can now be integrated on-chip capable of miniaturizing the FLIM system and making it a commercially viable tool for clinical use. This technology has the potential to revolutionize the current FLIM system with improved detection capabilities opening doors for new ho
People with brain or spinal cord-related paralysis often need to rely on others for basic tasks, limiting their independence. A potential solution is brain-machine interfaces (BMIs), which could allow them to voluntar...
People with brain or spinal cord-related paralysis often need to rely on others for basic tasks, limiting their independence. A potential solution is brain-machine interfaces (BMIs), which could allow them to voluntarily control external devices (e.g., robotic arm) by decoding brain activity to movement commands. In the past decade, deep-learning decoders have achieved state-of-the-art results in most BMI applications, ranging from speech production to finger control. However, the'black-box' nature of deep-learning decoders could lead to unexpected behaviors, resulting in major safety concerns in real-world physical control scenarios. In these applications, explainable but lower-performing decoders, such as the Kalman filter (KF), remain the norm. In this study, we designed a BMI decoder based on KalmanNet, an extension of the KF that augments its operation with recurrent neural networks to compute the Kalman gain. This results in a varying "trust" that shifts between inputs and dynamics. We used this algorithm to predict finger movements from the brain activity of two monkeys. We compared KalmanNet results offline (pre-recorded data, n = 13 days) and online (real-time predictions, n = 5 days) with a simple KF and two recent deep-learning algorithms: tcFNN (non-ReFIT version) and LSTM. KalmanNet achieved comparable or better results than other deep learning models in offline and online modes, relying on the dynamical model for stopping while depending more on neural inputs for initiating movements. We further validated this mechanism by implementing a heteroscedastic KF that used the same strategy, and it also approached state-of-the-art performance while remaining in the explainable domain of standard KFs. However, we also see two downsides to KalmanNet. KalmanNet shares the limited generalization ability of existing deep-learning decoders, and its usage of the KF as an inductive bias limits its performance in the presence of unseen noise distributions. Despite thi
OBJECTIVES: Alzheimer's Disease (AD) is one of the most common neurodegenerative diseases, resulting in progressive cognitive decline, and so accurate and timely AD diagnosis is of critical importance. To this end...
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OBJECTIVES: Alzheimer's Disease (AD) is one of the most common neurodegenerative diseases, resulting in progressive cognitive decline, and so accurate and timely AD diagnosis is of critical importance. To this end, various medical technologies and computer-aided diagnosis (CAD), ranging from biosensors and raw signals to medical imaging, have been used to provide information about the state of AD. In this survey, we aim to provide a review on CAD systems for automated AD detection, focusing on different data types: namely, signals and sensors, medical imaging, and electronic medical records (EMR). METHODS: We explored the literature on automated AD detection from 2022-2023. Specifically, we focused on various data resources and reviewed several preprocessing and learning methodologies applied to each data type, as well as evaluation metrics for model performance evaluation. Further, we focused on challenges, future perspectives, and recommendations regarding automated AD diagnosis. RESULTS: Compared to other modalities, medical imaging was the most common data type. The prominent modality was Magnetic Resonance Imaging (MRI). In contrast, studies based on EMR data type were marginal because EMR is mostly used for AD prediction rather than detection. Several challenges were identified: data scarcity and bias, imbalanced datasets, missing information, anonymization, lack of standardization, and explainability. CONCLUSION: Despite recent developments in automated AD detection, improving the trustworthiness and performance of these models, and combining different data types will improve usability and reliability of CAD tools for early AD detection in the clinical practice. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution License, permitting unrestricted use, distribution, and reproduction so long as the original work is properly cited. (https://***/licenses/by/4.0/).
This paper presents the algorithm and very-large-scale integration (VLSI) architecture of a high-throughput and highly efficient independent component analysis (ICA) processor for self-interference cancellation (SIC) ...
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An additional deposition step was added to a multi-step electron beam lithographic fabrication process to unlock the height dimension as an accessible parameter for resonators comprising unit cells of quasi-bound stat...
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An additional deposition step was added to a multi-step electron beam lithographic fabrication process to unlock the height dimension as an accessible parameter for resonators comprising unit cells of quasi-bound states in the continuum metasurfaces,which is essential for the geometric design of intrinsically chiral structures.
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