检索结果-内蒙古大学图书馆

Matrix Biology Plus 2025年 25卷 100167页

作者： Montgomery, Alyssa Westphal, Jennifer Bryan, Andrew E. Harris, Greg M. Department of Biomedical Engineering University of Cincinnati Cincinnati 45221 OH United States Department of Chemical and Environmental Engineering University of Cincinnati Cincinnati 45221 OH United States Neuroscience Graduate Program University of Cincinnati College of Medicine Cincinnati 45267 OH United States Cincinnati VA Medical Center Cincinnati 45220 OH United States

Schwann cells (SCs) hold key roles in axonal function and maintenance in the peripheral nervous system (PNS) and are a critical component to the regeneration process following trauma. Following PNS trauma, SCs respond to both physical and chemical signals to modify phenotype and assist in the regeneration of damaged axons and extracellular matrix (ECM). There is currently a lack of knowledge regarding the SC response to dynamic, temporal changes in the ECM brought on by swelling and the development of scar tissue as part of the body's wound-healing process. Thus, this work seeks to utilize a biocompatible, mechanically tunable biomaterial to mimic changes in the microenvironment following injury and over time. Previously, we have reported that ECM cues such as ligand type and substrate stiffness impact SC phenotype and plasticity, which was demonstrated by SCs on mechanically stable biomaterials. However, to better realize SC potential for plasticity following traumatic injury, a UV-tunable polydimethylsiloxane (PDMS) substrate with dynamically changing stiffness was utilized to mimic changes over time in the microenvironment. The dynamic biomaterial showed an increase in stress fibers, greater YAP expression, and fluctuations in c-Jun production in SCs in comparison to stiff and soft static controls. Utilizing biomaterials to better understand the role between temporal mechanical dynamics and SC phenotype holds a very high potential for developing future PNS therapies. © 2024

关键词： Biomaterials Extracellular matrix Fibrosis Mechanotransduction PNS injury Schwann cell

来源：评论

学校读者我要写书评

暂无评论

Safety,dosimetry,and efficacy of an optimized long-acting somatostatin analog for peptide receptor radionuclide therapy in metastatic neuroendocrine tumors:From preclinical testing to first-in-human study

引用

Acta Pharmaceutica Sinica B 2025年第2期15卷 707-721页

作者： Wei Guo Xuejun Wen Yuhang Chen Tianzhi Zhao Jia Liu Yucen Tao Hao Fu Hongjian Wang Weizhi Xu Yizhen Pang Liang Zhao Jingxiong Huang Pengfei Xu Zhide Guo Weibing Miao Jingjing Zhang Xiaoyuan Chen Haojun Chen Department of Nuclear Medicine and Minnan PET Center Xiamen Key Laboratory of Radiopharmaceuticalsthe First Affiliated Hospital of Xiamen UniversitySchool of MedicineXiamen UniversityXiamen 361102China School of Clinical Medicine Fujian Medical UniversityFuzhou 350005China State Key Laboratory of Molecular Vaccinology and Molecular Diagnostics&Center for Molecular Imaging and Translational Medicine School of Public HealthXiamen UniversityXiamen 361102China Departments of Diagnostic Radiology SurgeryChemical and Biomolecular Engineeringand Biomedical EngineeringYong Loo Lin School of Medicine and College of Design and EngineeringNational University of SingaporeSingapore 138667Singapore Clinical Imaging Research Centre Centre for Translational MedicineYong Loo Lin School of MedicineNational University of SingaporeSingapore 138667Singapore Nanomedicine Translational Research Program NUS Center for NanomedicineYong Loo Lin School of MedicineNational University of SingaporeSingapore 138667Singapore Department of Nuclear Medicine the First Affiliated HospitalFujian Medical UniversityFuzhou 350005China Department of Nuclear Medicine National Regional Medical CenterBinhai Campus of the First Affiliated HospitalFujian Medical UniversityFuzhou 350212China Fujian Key Laboratory of Precision Medicine for Cancer the First Affiliated HospitalFujian Medical UniversityFuzhou 350005China

Peptide receptor radionuclide therapy(PRRT)with radiolabeled SSTR2 agonists is a treatment option that is highly effective in controlling metastatic and progressive neuroendocrine tumors(NETs).Previous studies have shown that an SSTR2 agonist combined with albumin binding moiety Evans blue(denoted as^(177)Lu-EB-TATE)is characterized by a higher tumor uptake and residence time in preclinical models and in patients with metastatic *** study aimed to enhance the in vivo stability,pharmacokinetics,and pharmacodynamics of^(177)Lu-EB-TATE by replacing the maleimide-thiol group with a polyethylene glycol chain,resulting in a novel EB conjugated SSTR2-targeting radiopharmaceutical,^(177)Lu-LNC1010,for *** preclinical studies,^(177)Lu-LNC1010 exhibited good stability and SSTR2-binding affinity in AR42J tumor cells and enhanced uptake and prolonged retention in AR42J tumor ***,we presented the first-in-human dose escalation study of^(177)Lu-LNC1010 in patients with advanced/metastatic NETs.^(177)Lu-LNC1010 was well-tolerated by all patients,with minor adverse effects,and exhibited significant uptake and prolonged retention in tumor lesions,with higher tumor radiation doses than those of^(177)*** PRRT efficacy results showed an 83%disease control rate and a 42%overall response rate after two^(177)Lu-LNC1010 treatment *** encouraging findings warrant further investigations through multicenter,prospective,and randomized controlled trials.

关键词： Radiolabeled somatostatin analog Neuroendocrine tumor Evans blue ^(177)Lu-LNC1010 Peptide receptor radionuclide therapy First-in-human dose escalation study

来源：评论

学校读者我要写书评

暂无评论

Polybasic nanogels for intracellular co-delivery of paclitaxel and carboplatin: a novel approach to ovarian cancer therapy

RSC pharmaceutics

引用

RSC pharmaceutics 2025年第3期2卷 2025 Feb 21页

作者： Angela M Wagner Olivia L Lanier Ani Savk Nicholas A Peppas McKetta Department of Chemical Engineering The University of Texas at Austin Austin TX USA peppas@che.utexas.edu. Institute for Biomaterials Drug Delivery and Regenerative Medicine The University of Texas at Austin Austin TX USA. Department of Biomedical Engineering The University of Texas at Austin Austin TX USA. Department of Chemical and Biological Engineering University of New Mexico Albuquerque NM USA. Department of Biomedical Engineering University of New Mexico Albuquerque NM USA. Cancer Therapeutics Program University of New Mexico Comprehensive Cancer Center Albuquerque NM USA. Department of Surgery and Perioperative Care Dell Medical School University of Texas at Austin Austin TX USA. Department of Pediatrics Dell Medical School University of Texas at Austin Austin TX USA. Division of Molecular Pharmaceutics and Drug Delivery College of Pharmacy The University of Texas at Austin Austin TX USA.

Ovarian cancer is one of the leading causes of cancer-related deaths in women, with limited progress in treatments despite decades of research. Common treatment protocols rely on surgical removal of tumors and chemotherapy drugs, such as paclitaxel and carboplatin, which are capable of reaching cancer cells throughout the body. However, the effectiveness of these drugs is often limited due to toxic reactions in patients, nonspecific drug distribution affecting healthy cells, and the development of treatment resistance. In this study, we introduce a polybasic nanogel system composed of poly(diethylaminoethyl methacrylate--cyclohexyl methacrylate)--poly(ethylene glycol) designed for the targeted co-delivery of paclitaxel and carboplatin directly to ovarian cancer cells. These nanogel systems can respond to the cellular microenvironment to achieve controlled, on-demand drug release, reducing off-target effects and enhancing therapeutic uptake. Additionally, we investigated nanoparticle degradation and controlled drug release as a function of various crosslinkers, including tetraethylene glycol dimethacrylate, bis(2-methacryloyl)oxyethyl disulfide, poly(lactic acid)--poly(ethylene glycol)--poly(lactic acid)dimethacrylate, and polycaprolactone dimethacrylate. Our results, using OVCAR-3 human ovarian cancer cells, demonstrated that this dual-delivery system outperformed free drugs in inducing cancer cell death, representing a promising advance in the field of nanoparticle-based therapies for ovarian cancer. By loading two chemotherapeutic agents into a single, environmentally responsive particle, this approach shows the potential to overcome common resistance mechanisms and achieve more effective tumor suppression. In summary, by delivering chemotherapy more precisely, it may be possible to enhance therapeutic outcomes while minimizing toxicity and nonspecific drug distribution, ultimately improving patient quality of life.

关键词：

来源：评论

学校读者我要写书评

暂无评论

Fucoxanthin induces human melanoma cytotoxicity by thwarting the JAK2/STAT3/BCL-xL signaling axis

引用

Environmental Toxicology 2024年第6期39卷 3356-3366页

作者： Kuo, Min-Yung Dai, Wen-Chyi Chang, Jie-Li Chang, Jo-Shu Lee, Tse-Min Chang, Chia-Che Pediatric Surgery Division Department of Surgery Tungs' Taichung MetroHarbor Hospital Taichung Taiwan Doctoral Program in Biotechnology Industrial Innovation and Management National Chung Hsing University Taichung Taiwan Taichung Municipal Taichung First Senior High School Taichung Taiwan Department of Chemical and Materials Engineering Research Center for Smart Sustainable Circular Economy Tunghai University Taichung Taiwan Department of Life Sciences National Chung Hsing University Taichung Taiwan Department of Marine Biotechnology and Resources National Sun Yat-sen University Kaohsiung Taiwan Graduate Institute of Biomedical Sciences Master Program in Precision Health Doctoral Program in Translational Medicine Rong Hsing Research Center for Translational Medicine The iEGG and Animal Biotechnology Research Center National Chung Hsing University Taichung Taiwan Department of Medical Laboratory Science and Biotechnology Asia University Taichung Taiwan Department of Medical Research China Medical University Hospital Taichung Taiwan Traditional Herbal Medicine Research Center Taipei Medical University Hospital Taipei Taiwan

Melanoma is the most lethal skin malignancy. Fucoxanthin is a marine carotenoid with significant anticancer activities. Intriguingly, Fucoxanthin's impact on human melanoma remains elusive. Signal Transducer and Activator of Transcription 3 (STAT3) represents a promising target in cancer therapy due to its persistent activation in various cancers, including melanoma. Herein, we revealed that Fucoxanthin is cytotoxic to human melanoma cell lines A2758 and A375 while showing limited cytotoxicity to normal human melanocytes. Apoptosis is a primary reason for Fucoxanthin's melanoma cytotoxicity, as the pan-caspase inhibitor z-VAD-fmk drastically abrogated Fucoxanthin-elicited clonogenicity blockage. Besides, Fucoxanthin downregulated tyrosine 705-phosphorylated STAT3 (p-STAT3 (Y705)), either inherently present in melanoma cells or inducible by interleukin 6 (IL-6) stimulation. Notably, ectopic expression of STAT3-C, a dominant-active STAT3 mutant, abolished Fucoxanthin-elicited melanoma cell apoptosis and clonogenicity inhibition, supporting the pivotal role of STAT3 blockage in Fucoxanthin's melanoma cytotoxicity. Moreover, Fucoxanthin lowered BCL-xL levels by blocking STAT3 activation, while ectopic BCL-xL expression rescued melanoma cells from Fucoxanthin-induced killing. Lastly, Fucoxanthin was found to diminish the levels of JAK2 with dual phosphorylation at tyrosine residues 1007 and 1008 in melanoma cells, suggesting that Fucoxanthin impairs STAT3 signaling by blocking JAK2 activation. Collectively, we present the first evidence that Fucoxanthin is cytotoxic selectively against human melanoma cells while sparing normal melanocytes. Mechanistically, Fucoxanthin targets the JAK2/STAT3/BCL-xL antiapoptotic axis to provoke melanoma cell death. This discovery implicates the potential application of Fucoxanthin as a chemopreventive or therapeutic strategy for melanoma management. © 2024 Wiley Periodicals LLC.

关键词： Carotenoids

来源：评论

学校读者我要写书评

暂无评论

Tuning colloidal behavior of Ti3C2 MXene suspension toward designing functional biohybrids

Tuning colloidal behavior of Ti3C2 MXene suspension toward d...

引用

2020 AIChE Annual Meeting

作者： Gholamirad, Farivash Taheri-Qazvini, Nader Department of Chemical Engineering University of South Carolina ColumbiaSC United States Biomedical Engineering Program University of South Carolina ColumbiaSC United States

engineering the assembly of MXene nanosheets with positively charged biopolymers, Combining the functionality of biopolymers with MXene, Expanding the applications of MXene by designing complex structures. © 2020... 详细信息

ISBN: (纸本)9780816911141

关键词： Biopolymers

来源：评论

学校读者我要写书评

暂无评论

Mechanobiology of Collective Cell Migration in 3D Microenvironments

arXiv

引用

arXiv 2022年

作者： Hruska, Alex M. Yang, Haiqian Leggett, Susan E. Guo, Ming Wong, Ian Y. School of Engineering Center for Biomedical Engineering Joint Program in Cancer Biology Brown University ProvidenceRI02912 United States Department of Mechanical Engineering Massachusetts Institute of Technology CambridgeMA02139 United States Department of Chemical and Biological Engineering Princeton University Princeton NJ08540 United States

Tumor cells invade individually or in groups, mediated by mechanical interactions between cells and their surrounding matrix. These multicellular dynamics are reminiscent of leader-follower coordination and epithelial-mesenchymal transitions (EMT) in tissue development, which may occur via dysregulation of associated molecular or physical mechanisms. However, it remains challenging to elucidate such phenotypic heterogeneity and plasticity without precision measurements of single cell behavior. The convergence of technological developments in live cell imaging, biophysical measurements, and 3D biomaterials are highly promising to reveal how tumor cells cooperate in aberrant microenvironments. Here, we highlight new results in collective migration from the perspective of cancer biology and bioengineering. First, we review the biology of collective cell migration. Next, we consider physics-inspired analyses based on order parameters and phase transitions. Further, we examine the interplay of metabolism and heterogeneity in collective migration. We then review the extracellular matrix, and new modalities for mechanical characterization of 3D biomaterials. We also explore epithelial-mesenchymal plasticity and implications for tumor progression. Finally, we speculate on future directions for integrating mechanobiology and cancer cell biology to elucidate collective migration. © 2022, CC BY-NC-ND.

关键词： Cytology

来源：评论

学校读者我要写书评

暂无评论

Machine-learned molecular surface and its application to implicit solvent simulations

arXiv

引用

arXiv 2021年

作者： Wei, Haixin Zhao, Zekai Luo, Ray Departments of Materials Science and Engineering Molecular Biology and Biochemistry Chemical and Biomolecular Engineering and Biomedical Engineering Graduate Program in Chemical and Materials Physics University of California IrvineCA92697 United States

Implicit solvent models, such as Poisson-Boltzmann models, play important roles in computational studies of biomolecules. A vital step in almost all implicit solvent models is to determine the solvent-solute interface, and the solvent excluded surface (SES) is the most widely used interface definition in these models. However, classical algorithms used for computing SES are geometry-based, thus neither suitable for parallel implementations nor convenient for obtaining surface derivatives. To address the limitations, we explored a machine learning strategy to obtain a level-set formulation for the SES. The training process was conducted in three steps, eventually leading to a model with over 95% agreement with the classical SES. Visualization of tested molecular surfaces shows that the machine-learned SES overlaps with the classical SES on almost all situations. We also implemented the machine-learned SES into the Amber/PBSA program to study its performance on reaction field energy calculation. The analysis shows that the two sets of reaction field energies are highly consistent with 1% deviation on average. Given its level-set formulation, we expect the machine-learned SES to be applied in molecular simulations that require either surface derivatives or high efficiency on parallel computing platforms. © 2021, CC BY.

关键词： Solvents

来源：评论

学校读者我要写书评

暂无评论

Advances in transcription factor delivery: Target selection, engineering strategies, and delivery platforms

引用

Journal of Controlled Release 2025年 384卷 113885页

作者： Lee, Yeji Yoo, Seongkyeong Cho, Seongeon Kim, Iljin Kim, In-San KU-KIST Graduate School of Converging Science and Technology Korea University Seoul02841 Korea Republic of Chemical and Biological Integrative Research Center Biomedical Research Institute Korea Institute Science and Technology Seoul02792 Korea Republic of Department of Pharmacology and Program in Biomedical Science and Engineering Inha University College of Medicine Incheon22212 Korea Republic of Research Center for Controlling Intercellular Communication Inha University College of Medicine Incheon22212 Korea Republic of

Recent advances in delivery systems for transcription factors (TFs) have opened new therapeutic opportunities in regenerative medicine, cancer therapy, and genetic disorders. However, effective TF delivery still faces substantial obstacles, including limited cellular uptake, inefficient nuclear translocation, low cargo stability, and insufficient target specificity. Furthermore, artificial TFs have enabled targeted modulation of gene expression, further expanding their therapeutic potential. This review comprehensively discusses current progress in TF delivery methodologies, including direct TF protein delivery using cell-penetrating peptides, and extracellular vesicles, as well as TF gene delivery approaches utilizing both lipid-based nanoparticles and viral strategies. Notably, engineered nanoparticles have emerged as promising platforms due to their precise control over TF delivery, improved specificity, and minimized off-target effects. Despite these significant advancements, major hurdles in delivery efficiency, cargo stability, and overall safety persist. Overcoming these obstacles will be essential to accelerate the clinical translation of TF-based therapeutics for a broad spectrum of diseases. © 2024

关键词： Transcription factors

来源：评论

学校读者我要写书评

暂无评论

Stellate silicon microneedles for rapid point-of-care melanoma exosome isolation and detection via a lateral flow assay

引用

Biosensors and Bioelectronics 2025年 285卷 117560页

作者： Smith, Scott M. Kumari, Abha Marvar, Joseph P. Onukwugha, Nna-Emeka Kang, Yoon-Tae Nagrath, Sunitha Department of Chemical Engineering and Biomedical Engineering Biointerfaces Institute Translational Oncology Program University of Michigan Ann ArborMI48109 United States

Melanoma is the most aggressive type of skin cancer with high mortality rates. Early diagnosis is crucial because it significantly improves treatment outcomes, but conventional methods relying on dermoscopy and lesion biopsy have limitations in accuracy during early stages and are invasive. Liquid biopsies offer a minimally invasive alternative, particularly for routine screening. The abundance of cancer cell-driven extracellular vesicles in interstitial fluid can be utilized for point-of-care cancer diagnostics. Here, we developed a stellate silicon microneedle patch, the ExoPatch, coated with Annexin V functionalized hydrogel to isolate melanoma-specific exosomes. The ExoPatch captures exosomes directly from the skin, followed by dissolution of the hydrogel to release the exosomes, which are then detected using a lateral flow immunoassay specific to melanoma markers (MCAM and MCSP). After validating with cell line derived extracellular vesicles and testing with mouse tissue, the ExoPatch isolated 11.5 times more protein from melanoma tissue compared to healthy tissue. Additionally, the ExoPatch effectively distinguished between melanoma and healthy tissues, with its specificity confirmed through Western Blot and electron microscopy analysis. The ExoPatch with melanoma mouse samples produced a 3.5-fold higher signal in the lateral flow immunoassay compared to that of healthy controls. The ExoPatch presents a promising point-of-care diagnostic tool for melanoma, offering significant advantages in terms of rapidness, minimal invasiveness, and ease of use. It has the potential to enhance early detection and routine monitoring in melanoma patients, ultimately improving patient outcomes by reducing the reliance on traditional, invasive biopsies. © 2025

关键词： Biopsy

来源：评论

学校读者我要写书评

暂无评论

Multi-Aptamer Targeting of Listeria Monocytogenes in Agricultural Waters Using Platinum-Coated Laser Inscribed Graphene Biosensors

SSRN

引用

SSRN 2023年

作者： Cavallaro, Nicholas Xiang, Dong Datta, Shoumen P.A. Gomes, Carmen McLamore, Eric S. Agricultural and Biological Engineering Institute of Food and Agricultural Sciences University of Florida United States School of Chemistry Chemical Engineering and Biotechnology Nanyang Technological University Singapore MIT Auto-ID Labs Department of Mechanical Engineering Massachusetts Institute of Technology United States MDPnP Labs Biomedical Engineering Program Department of Anesthesiology Massachusetts General Hospital Harvard Medical School United States Department of Mechanical Engineering Iowa State University United States Agricultural Sciences Clemson University United States

Fresh produce is often associate with foodborne illnesses. Listeria monocytogenes is a source of fresh produce contamination. L. monocytogenes is among the leading causes of severe foodborne illnesses in the United States, with a 16% mortality rate. The organism can survive in a wide range of conditions and is known to form biofilms on different surfaces, which poses challenges in monitoring and prevention. Tools for rapid monitoring are needed for transient pathogens such as L. monocytogenes to prevent outbreaks. In this manuscript, we demonstrated the feasibility of the multi-aptamer approach for development of label-free aptasensors targeting Listeria monocytogenes in irrigation water for lettuce production. We use in silico modeling and experimental validation studies with surface plasmon resonance to rationally develop mixtures of relevant aptamers (including scrambled aptamers as negative control). Based on this screening, multiple aptamers targeting extracellular structures on intact L. monocytogenes were tethered to platinum-modified laser inscribed graphene electrodes. We show that mixing multiple aptamers with varying affinity improves the diagnostic performance in complex sample matrices (lettuce hydroponic water). In a challenge study, multi-aptamer biosensors showed enhanced sensitivity, higher accuracy (>85%), improved limit of detection (10 CFU/mL), and improved selectivity over single aptamer biosensors. Rapid screening of pathogens in agricultural water is a global need to meet food safety and water quality regulatory needs. Given the diversity of extracellular targets on intact bacteria, this strategy could be further extended by including additional aptamers (both positive and negative control), as well as other mechanisms for account for non-specific binding. © 2023, The Authors. All rights reserved.

关键词： Lettuce

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：