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Npj imaging 2024年第1期2卷 12页

作者： Yohana C Toner Geoffrey Prévot Mandy M T van Leent Jazz Munitz Roderick Oosterwijk Anna Vera D Verschuur Yuri van Elsas Vedran Peric Rianne J F Maas Anna Ranzenigo Judit Morla-Folch William Wang Martin Umali Anne de Dreu Jessica Chimene Fernandes Nathaniel A T Sullivan Alexander Maier Christian Mason Thomas Reiner Zahi A Fayad Willem J M Mulder Abraham J P Teunissen Carlos Pérez-Medina BioMedical Engineering and Imaging Institute Icahn School of Medicine at Mount Sinai New York NY USA. Department of Diagnostic Molecular and Interventional Radiology Icahn School of Medicine at Mount Sinai New York NY USA. Department of Internal Medicine and Radboud Center for Infectious Diseases Radboud University Medical Center Nijmegen the Netherlands. Cardiovascular Research Institute Icahn School of Medicine at Mount Sinai New York NY USA. Laboratory of Chemical Biology Department of Biomedical Engineering Eindhoven University of Technology Eindhoven The Netherlands. Department of Cardiology and Angiology Heart Center Freiburg University Faculty of Medicine University of Freiburg Freiburg Germany. Department of Radiology Memorial Sloan Kettering Cancer Center New York NY USA. Department of Radiology Weill Cornell Medical College New York NY USA. Chemical Biology Program Memorial Sloan Kettering Cancer Center New York NY USA. Icahn Genomics Institute Icahn School of Medicine at Mount Sinai New York NY USA. Centro Nacional de Investigaciones Cardiovasculares (CNIC) Madrid Spain.

Macrophages are key inflammatory mediators in many pathological conditions, including cardiovascular disease (CVD) and cancer, the leading causes of morbidity and mortality worldwide. This makes macrophage burden a valuable diagnostic marker and several strategies to monitor these cells have been reported. However, such strategies are often high-priced, non-specific, invasive, and/or not quantitative. Here, we developed a positron emission tomography (PET) radiotracer based on apolipoprotein A1 (ApoA1), the main protein component of high-density lipoprotein (HDL), which has an inherent affinity for macrophages. We radiolabeled an ApoA1-mimetic peptide (mA1) with zirconium-89 (Zr) to generate a lipoprotein-avid PET probe (Zr-mA1). We first characterized Zr-mA1's affinity for lipoproteins in vitro by size exclusion chromatography. To study Zr-mA1's in vivo behavior and interaction with endogenous lipoproteins, we performed extensive studies in wildtype C57BL/6 and hypercholesterolemic mice. Subsequently, we used in vivo PET imaging to study macrophages in melanoma and myocardial infarction using mouse models. The tracer's cell specificity was assessed by histology and mass cytometry (CyTOF). Our data show that Zr-mA1 associates with lipoproteins in vitro. This is in line with our in vivo experiments, in which we observed longer Zr-mA1 circulation times in hypercholesterolemic mice compared to C57BL/6 controls. Zr-mA1 displayed a tissue distribution profile similar to ApoA1 and HDL, with high kidney and liver uptake as well as substantial signal in the bone marrow and spleen. The tracer also accumulated in tumors of melanoma-bearing mice and in the ischemic myocardium of infarcted animals. In these sites, CyTOF analyses revealed that Zr-mA1 was predominantly taken up by macrophages. Our results demonstrate that Zr-mA1 associates with lipoproteins and hence accumulates in macrophages in vivo. Zr-mA1's high uptake in these cells makes it a promising radiotracer for non-

关键词： biomedical engineering Imaging techniques and agents Positron-emission tomography

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Extraction of PLA2 and antibacterial activity test of lionfish (Pterois volitans) spine venom

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IOP Conference Series: Earth and Environmental Science 2020年第1期462卷

作者： A N Sommeng M Y A Ramadhan R Larasati M J Ginting M Sahlan H Hermansyah A Wijanarko Department of Chemical Engineering Faculty of Engineering Universitas Indonesia Depok 16424 Indonesia Marine Science Postgraduate Program Faculty of Mathematics and Natural Science Universitas Indonesia Depok 16424 Indonesia Research Centre for Biomedical Engineering Faculty of Engineering Universitas Indonesia Depok 16424 Indonesia

The population of Pterois volitans has caused significant damage to other fish populations and coral reef ecosystems. Population control of P. volitans consumes a considerable cost so that the utilization of these fish needs to be sought to be useful along with controlling the population. This fish is known to contain the enzyme Phospholipase A2 (PLA2), which used as an antibiotic against some bacteria. This study will examine the antibacterial activity of the phospholipase A2 enzyme extracted from P. volitans venom to Escherichia coli, Bacillus subtilis, and Staphylococcus aureus. The method used to isolate the enzyme PLA2 is by using precipitation of ammonium sulfate (AS) and precipitation with ethanol. The results of the precipitation tested with the Lowry protein concentration test, the Marinetti PLA2 activity test, and the identification of the SDS-PAGE protein. The agar diffusion disc method is used to test the antibacterial activity. The results obtained from this research are that 80% ammonium sulfate precipitation method has the highest protein and enzyme activity with a ratio of 1.32 times compared to toxic extract. For antibacterial activity test results, an 80% ammonium sulfate sample may inhibit the activity of S. aureus bacteria but does not affect B. subtilis and E. coli.

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Protein isolation and identification of Pterois volitans spine venom coagulant activity

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IOP Conference Series: Earth and Environmental Science 2020年第1期462卷

作者： A N Sommeng A K Eka M Y A Ramadhan M J Ginting M Sahlan H Hermansyah A Wijanarko Department of Chemical Engineering Faculty of Engineering Universitas Indonesia Depok 16424 Indonesia Marine Science Postgraduate Program Faculty of Mathematics and Natural Science Universitas Indonesia Depok 16424 Indonesia Research Centre for Biomedical Engineering Faculty of Engineering Universitas Indonesia Depok 16424 Indonesia

Pterois volitans, or commonly referred to lionfish, are fish species originating from Indo-Pacific waters but are becoming invasive in other regions such as the Caribbean and Atlantis. Various efforts have been made to reduce the number of lionfish, and one of them is by utilizing the venom on the spine. The venom extraction of P. volitans spines is done mechanically using sonication and centrifugation, and then protein isolation is carried out using salt. Coagulant activity from extract (crude venom) and lionfish venom protein isolate was done by counting PT (prothrombin time) and aPTT (activated partial thromboplastin time) which resulted that the crude venom and protein isolate of lionfish venom can accelerate blood clot (procoagulant) respectively up to 8.5 seconds and 6 seconds. Protein identification was made using LC-MS/MS device. The LC-MS/MS analysis showed that the protein isolate of lionfish venom contains Nomega-nitro-L-arginine methyl ester (L-NAME) compounds known to have procoagulant effects. From a series of tests mentioned, it concluded that P. volitans venom have procoagulant activity and one of the compounds responsible for it is L-NAME

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Protein isolation of Pterois volitans venomous with a heating process for antibacterial activity assay

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IOP Conference Series: Earth and Environmental Science 2020年第1期462卷

作者： A N Sommeng F Riswandha M J Ginting S Pebriani M Sahlan H Hermansyah A Wijanarko Department of Chemical Engineering Faculty of Engineering Universitas Indonesia Depok 16424 Indonesia Marine Science Postgraduate Program Faculty of Mathematics and Natural Science Universitas Indonesia Depok 16424 Indonesia Research Centre for Biomedical Engineering Faculty of Engineering Universitas Indonesia Depok 16424 Indonesia

Pterois volitans is an invasive native predatory fish species in the Indo-Pacific ocean that disrupt the food chain and damage coral reefs which cause ecosystem imbalances. These fish have venomous spines that made them inedible and avoided by a predator. Due to its rapid growth, utilization of this venom can be useful along with controlling the population. Recent studies show the benefits of phospholipase A2, which is a protein compound that contained in the venom has antibacterial activity and expected to be an antibacterial agent. In this study, we used heating and gradual purification to obtain the optimum concentration of Phospholipase A2. The protein isolates were analyzed using the Marinetti method, determination of concentration by Lowry test, identification of protein with SDS-Page, and the antibacterial activity test using agar diffusion. The results of phospholipase A2 obtained from the extract of P. volitans venom by purification method ammonium sulfate at 80% saturation with a heating time of 35 minutes had a specific enzyme activity of 0.0206 units / µg and can inhibit E. coli bacteria 98.81% and Salmonella sp. inhibit 89.28% with a concentration of 3.77 µg / ml.

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Phase-change metal ink with pH-controlled chemical sintering for versatile and scalable fabrication of variable stiffness electronics

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Science advances 2025年第22期11卷 eadv4921页

作者： Simok Lee Gun-Hee Lee Inho Kang Woojin Jeon Semin Kim Yejin Ahn Choong Yeon Kim Do A Kwon Michael D Dickey Steve Park Seongjun Park Jae-Woong Jeong School of Electrical Engineering Korea Advanced Institute of Science and Technology (KAIST) 291 Daehak-ro Yuseong-gu Daejeon 34141 Republic of Korea. Medical Research Center Seoul National University Seoul 03080 Republic of Korea. Departments of Cogno-Mechatronics Engineering and Optics and Mechatronics Engineering Pusan National University Busan 46241 Republic of Korea. Graduate School of Semiconductor Technology Korea Advanced Institute of Science and Technology (KAIST) 291 Daehak-ro Yuseong-gu Daejeon 34141 Republic of Korea. KAIST Information and Electronics Research Institute Daejeon 34141 Republic of Korea. Research Laboratory of Electronics Massachusetts Institute of Technology (MIT) Cambridge MA 02139 USA. Department of Mechanical Engineering Massachusetts Institute of Technology (MIT) Cambridge MA 02139 USA. Department of Chemical and Biomolecular Engineering North Carolina State University (NCSU) Raleigh NC 27606 USA. Department of Materials Science and Engineering Korea Advanced Institute of Science and Technology (KAIST) 291 Daehak-ro Yuseong-gu Daejeon 34141 Republic of Korea. KAIST Institute for NanoCentury Daejeon 34141 Republic of Korea. Department of Biomedical Sciences College of Medicine Seoul National University Seoul 03080 Republic of Korea. School of Transdisciplinary Innovations Seoul National University Seoul 03080 Republic of Korea. Interdisciplinary Program in Bioengineering College of Engineering Seoul National University Seoul 08826 Republic of Korea. Department of Transdisciplinary Medicine Seoul National University Hospital Seoul 03080 Republic of Korea. KAIST Institute for Health Science and Technology Daejeon 34141 Republic of Korea.

Variable stiffness electronics represent the forefront of adaptive technology, integrating rigid and soft electronics in a single system through dynamic mechanical modulation. While gallium's high modulus tuning ratio and rapid phase transitions make it ideal for transformative electronic systems (TES), its liquid-state instability, high surface tension, and unintended phase transitions during processing pose substantial challenges. Here, we introduce STiffness-Adjustable temperature-Responsive ink (STAR ink), a chemically sinterable gallium composite electronic ink designed to overcome these obstacles. STAR ink enables high-resolution (~50 micrometers) circuit patterning, large-scale batch fabrication, and three-dimensional structure coating at room temperature. Through pH-controlled chemical sintering, STAR ink-based TES exhibits exceptional mechanical tunability (tuning ratio: 1465) and electrical conductivity (2.27 × 10 siemens per meter). Demonstrated applications-from multilayered variable stiffness printed circuit boards (PCBs) matching standard PCBs' complexity to body-temperature responsive neural probe-underscore STAR ink's potential for reconfigurable electronics across consumer electronics and biomedical devices.

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Corrigendum to "Elucidating the crosstalk between endothelial-to-mesenchymal transition (EndoMT) and endothelial autophagy in the pathogenesis of atherosclerosis" [Vascular Pharmacology 155 (2024) 107368]

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Vascular pharmacology 2024年 155卷 107373页

作者： Bandana Singh Kui Cui Shahram Eisa-Beygi Bo Zhu Douglas B Cowan Jinjun Shi Chris A Bashur Da-Zhi Wang Zhenguo Liu Joyce Bischoff Hong Chen Vascular Biology Program Department of Surgery Harvard Medical School Boston Children's Hospital Boston MA USA. Department of Anesthesiology Perioperative and Pain Medicine Brigham and Women's Hospital Boston MA USA. Department of Biomedical Chemical Engineering and Science Florida Institute of Technology 150 West University Blvd. Melbourne FL USA. Center for Regenerative Medicine University of South Florida Health Heart Institute Morsani College of Medicine University of South Florida Tampa FL USA. Division of Cardiovascular Medicine Department of Medicine University of Missouri School of Medicine Columbia MO USA. Vascular Biology Program Department of Surgery Harvard Medical School Boston Children's Hospital Boston MA USA. Electronic address: hong.chen@childrens.harvard.edu.

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Sensitivity analysis of gas distribution pipeline risk assessment methodology in Indonesia

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AIP Conference Proceedings 2020年第1期2230卷

作者： Andy Noorsaman Sommeng Fristy Rizcky Nurzaneal Mikael Januardi Ginting Sonya Pebriani Muhamad Sahlan Heri Hermansyah Anondho Wijanarko 1Department of Chemical Engineering Faculty of Engineering Universitas Indonesia Depok Indonesia 2Marine Science Postgraduate Program Faculty of Mathematics and Natural Science Universitas Indonesia Depok Indonesia 3Research Centre for Biomedical Engineering Faculty of Engineering Universitas Indonesia Depok Indonesia

Sensitivity is the effect of changes in any variables on changes in the risk results. The most common Risk Assessment Methodology in Indonesia is Muhlbauer, Modified Muhlbauer & Risk-Based Inspection. This study evaluated six natural gas distribution pipeline segments. In general, there are two objectives, firstly comparing the risk result between Risk Assessment Methodology. The following objectives are to compare the sensitivity. The Risk Assessments methodology is translated into a mathematic model and computed in Monte Carlo based simulation software. The risk value result shows that in high risk & medium risk pipeline segments, all methodology is valid with 24% and 13% deviation, respectively. The sensitivity analysis result shows that Muhlbauer & Modified Muhlbauer’s methodology is sensitive to third party activity. Risk-Based Inspection is sensitive to intrinsic damage mechanisms. This sensitivity data can be adopted to develop a more sensitive Risk Assessment Methodology for gas pipeline in the future.

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The Role of Synthetic Biology in Atmospheric Greenhouse Gas Reduction: Prospects and Challenges

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BioDesign Research 2020年第1期2020卷 1-8页

作者： Charles DeLisi Aristides Patrinos Michael MacCracken Dan Drell George Annas Adam Arkin George Church Robert Cook-Deegan Henry Jacoby Mary Lidstrom Jerry Melillo Ron Milo Keith Paustian John Reilly Richard J.Roberts Daniel Segrè Susan Solomon Dominic Woolf Stan D.Wullschleger Xiaohan Yang Department of Biomedical Engineering and Program in Bioinformatics College of EngineeringBoston UniversityBoston MA 02215USA The NOVIM Group Kohn HallUC Santa BarbaraCA 93106USA Climate Institute WashingtonDCUSA Department of Energy WashingtonDCUSA Center for Health Law Ethics&Human Rights at the Boston University School of Public HealthSchool of MedicineBoston UniversityUSA Department of Bioengineering University of CaliforniaBerkeley CAUSA Department of Genetics Harvard Medical SchoolCambridge MAUSA School for the Future of Innovation in Society Arizona State UniversityBarrett&O’Connor Washington Center1800 I StreetNWWashingtonDC 20006USA Sloan School of Management MITCambridge MAUSA Department of Chemical Engineering University of WashingtonSeattle WashingtonUSA The Ecosystems Center of the Marine Biological Laboratory in Woods Hole MAUSA Department of Plant and Environmental Sciences Weizmann Institute of ScienceRehovotIsrael Department of Soil and Crop Sciences Colorado State UniversityFort Collins CO 80523USA MIT Joint Program on the Science and Policy of Global Change MITCambridge MAUSA New England Biolabs Beverly MAUSA Department of Biology and Program in Bioinformatics Boston UniversityBoston MA 02215USA Department of Earth Atmospheric and Planetary SciencesMITCambridge MAUSA Soil and Crop Sciences Section School of Integrated Plant SciencesCornell UniversityIthaca NYUSA Environmental Sciences Division Oak Ridge National LaboratoryOak Ridge TNUSA Biosciences Division Oak Ridge National LaboratoryOak RidgeTNUSA

The long atmospheric residence time of CO2 creates an urgent need to add atmospheric carbon drawdown to CO2 regulatory *** and systems biology(SSB),which enables manipulation of cellular phenotypes,offers a powerful approach to amplifying and adding new possibilities to current land management practices aimed at reducing atmospheric *** participants(in attendance:Christina Agapakis,George Annas,Adam Arkin,George Church,Robert Cook-Deegan,Charles DeLisi,Dan Drell,Sheldon Glashow,Steve Hamburg,Henry Jacoby,Henry Kelly,Mark Kon,Todd Kuiken,Mary Lidstrom,Mike MacCracken,June Medford,Jerry Melillo,Ron Milo,Pilar Ossorio,Ari Patrinos,Keith Paustian,Kristala Jones Prather,Kent Redford,David Resnik,John Reilly,Richard ***,Daniel Segre,Susan Solomon,Elizabeth Strychalski,Chris Voigt,Dominic Woolf,Stan Wullschleger,and Xiaohan Yang)identified a range of possibilities by which SSB might help reduce greenhouse gas concentrations and which might also contribute to environmental sustainability and *** include,among other possibilities,engineering plants to convert CO2 produced by respiration into a stable carbonate,designing plants with an increased root-to-shoot ratio,and creating plants with the ability to self-fertilize.A number of serious ecological and societal challenges must,however,be confronted and resolved before any such application can be fully assessed,realized,and deployed.

关键词： Synthetic Roberts enable

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Erratum: Superhydrophobic polyether sulfone (PES) dialysis membrane with enhanced hemocompatibility and reduced human serum protein interactions: Ex vivo, in situ synchrotron imaging, experimental, and computational studies (Separation and Purification Technology (2024) 335, (S1383586623029799), (10.1016/***.2023.126071))

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Separation and Purification Technology 2025年

作者： Kalugin, Denis Bahig, Jumanah Shoker, Ahmed Doan, Huu Kirychuk, Shelley Abdelrasoul, Amira Department of Chemical and Biological Engineering University of Saskatchewan 57 Campus Drive SaskatoonSKS7N 5A9 Canada Division of Biomedical Engineering University of Saskatchewan 57 Campus Drive SaskatoonSKS7N 5A9 Canada Kinesiology University of Saskatchewan 87 Campus Dr SaskatoonSKS7N 5B Canada Saskatchewan Transplant Program St. Paul's Hospital 1702 20th Street West Saskatoon SaskatchewanS7M 0Z9 Canada Nephrology Division College of Medicine University of Saskatchewan 107 Wiggins Rd SaskatoonSKS7N 5E5 Canada Chemical Engineering Department Toronto Metropolitan University 350 Victoria St. TorontoONM5B 2K3 Canada Department of Medicine College of Medicine Canadian Centre for Rural and Agricultural Health University of Saskatchewan 107 Wiggins Rd SaskatoonSKS7N 5E5 Canada National Agricultural Industrial Hygiene Laboratory University of Saskatchewan 104 Clinic Place SaskatoonSKS7N 2Z4 Canada

The captions for the figures have been updated as follows: Fig 4a: AFM image of neat PES membrane [55]. Fig 8a: AFM image of neat PES membrane after interactions with FB [55]. Fig. 9a: DSC peak of neat PES membrane [5...

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Spatiotemporal Control of Morphogen Delivery to Pattern Stem Cell Differentiation in Three-Dimensional Hydrogels

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Current Protocols in Stem Cell Biology 2019年第1期51卷 e97页

作者： Balikov, Daniel A. Lippmann, Ethan S. Bellan, Leon M. Department of Mechanical Engineering Vanderbilt University Nashville TN United States Interdisciplinary Materials Science Program Vanderbilt University Nashville TN United States Department of Biomedical Engineering Vanderbilt University Nashville TN United States Department of Chemical and Biomolecular Engineering Vanderbilt University Nashville TN United States

Morphogens are biological molecules that alter cellular identity and behavior across both space and time. During embryonic development, morphogen spatial localization can be confined to small volumes in a single tissue or permeate throughout an entire organism, and the temporal effects of morphogens can range from fractions of a second to several days. In most cases, morphogens are presented as a gradient to adjacent cells within tissues to pattern cell fate. As such, to appropriately model development and build representative multicellular architectures in vitro, it is vital to recapitulate these gradients during stem cell differentiation. However, the ability to control morphogen presentation within in vitro systems remains challenging. Here, we describe an innovative platform using channels patterned within thick, three-dimensional hydrogels that deliver multiple morphogens to embedded cells, thereby demonstrating exquisite control over both spatial and temporal variations in morphogen presentation. This generalizable approach should have broad utility for researchers interested in patterning in vitro tissue structures. © 2019 by John Wiley & Sons, Inc. © 2019 John Wiley & Sons, Inc.

关键词： differentiation gradient hydrogel morphogen stem cell tissue engineering

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