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Ligand-directed profiling of organelles with internalizing phage libraries

Current Protocols in Protein Science 2015年第1期2015卷 30.4.1-30.4.30页

作者： Dobroff, Andrey S. Range, Roberto Guzman-Roja, Liliana Salmeron, Carolina C. Gelovani, Juri G. Sidman, Richard L. Bologa, Cristian G. Oprea, Tudor I. Brinker, C. Jeffrey Pasqualini, Renata Arap, Wadih Division of Hematology/Oncology Department of Internal Medicine University of New Mexico School of Medicine Albuquerque NM United States Division of Molecular Medicine Department of Internal Medicine University of New Mexico School of Medicine Albuquerque NM United States Cancer Research Program Houston Methodist Research Institute Houston TX United States Department of Biomedical Engineering Wayne State University Detroit MI United States Department of Neurology Beth Israel Deaconess Medical Center Harvard Medical School Boston MA United States Translational Informatics Division Department of Internal Medicine University of New Mexico School of Medicine Albuquerque NM United States Department of Chemical and Nuclear Engineering The University of New Mexico Cancer Center Albuquerque NM United States

Phage display is a resourceful tool to, in an unbiased manner, discover and characterize functional protein-protein interactions, create vaccines, and engineer peptides, antibodies, and other proteins as targeted diagnostic and/or therapeutic agents. Recently, our group has developed a new class of internalizing phage (iPhage) for ligand-directed targeting of organelles and to identify molecular pathways within live cells. This unique technology is suitable for applications ranging from fundamental cell biology to drug development. This unit describes the methods for generating and screening the iPhage display system, and explains how to select and validate candidate internalizing homing peptide. © 2014 by John Wiley & Sons, Inc.

关键词： Intracellular receptors Intracellular targeting Mammalian cells Organelles Penetratin Peptides Phage display Proteomics

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Corrigendum to “Room-temperature cured hydrophobic epoxy/graphene composites as corrosion inhibitor for cold-rolled steel” [Carbon 66 (2014) 144–153]

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Carbon 2015年 82卷 611-611页

作者： Kung-Chin Chang Min-Hsiang Hsu Hsin-I Lu Mei-Chun Lai Pei-Ju Liu Chien-Hua Hsu Wei-Fu Ji Tsao-Li Chuang Yen Wei Jui-Ming Yeh Wei-Ren Liu Department of Chemistry Center for Nanotechnology and Institute of Biomedical Technology at Chung at Yuan Christian University (CYCU) Chung Li 32023 Taiwan ROC Master Program in Nanotechnology and Center for Nanotechnology at CYCU Chung Li 32023 Taiwan ROC Department of Chemistry and Key Lab of Organic Optoelectronic & Molecular Engineering of Ministry of Education Tsinghua University Beijing 100084 China Department of Chemical Engineering CYCU Chung Li 32023 Taiwan ROC

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Use of culture geometry to control hypoxia-induced vascular endothelial growth factor secretion from adipose-derived stem cells: Optimizing a cell-based approach to drive vascular growth

Use of culture geometry to control hypoxia-induced vascular ...

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作者： Skiles, Matthew L. Sahai, Suchit Rucker, Lindsay Blanchette, James O. Biomedical Engineering Program College of Engineering and Computing University of South Carolina 301 Main Street Columbia SC 29208 United States Department of Chemical Engineering College of Engineering and Computing University of South Carolina Columbia SC United States

Adipose-derived stem cells (ADSCs) possess potent angiogenic properties and represent a source for cell-based approaches to delivery of bioactive factors to drive vascularization of tissues. Hypoxic signaling appears to be largely responsible for triggering release of these angiogenic cytokines, including vascular endothelial growth factor (VEGF). Three-dimensional (3D) culture may promote activation of hypoxia-induced pathways, and has furthermore been shown to enhance cell survival by promoting cell-cell interactions while increasing angiogenic potential. However, the development of hypoxia within ADSC spheroids is difficult to characterize. In the present study, we investigated the impact of spheroid size on hypoxia-inducible transcription factor (HIF)-1 activity in spheroid cultures under atmospheric and physiological oxygen conditions using a fluorescent marker. Hypoxia could be induced and modulated by controlling the size of the spheroid;HIF-1 activity increased with spheroid size and with decreasing external oxygen concentration. Furthermore, VEGF secretion was impacted by the hypoxic status of the culture, increasing with elevated HIF-1 activity, up to the point at which viability was compromised. Together, these results suggest the ability to use 3D culture geometry as a means to control output of angiogenic factors from ADSCs, and imply that at a particular environmental oxygen concentration an optimal culture size for cytokine production exists. Consideration of culture geometry and microenvironmental conditions at the implantation site will be important for successful realization of ADSCs as a pro-angiogenic therapy. © Mary Ann Liebert, Inc.

关键词： Geometry

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CBIO-23THE TEAD4-DRIVEN TRANSPORTOME MEDIATES ACTIVATION OF THE OSMOTIC ENGINE TO POTENTIATE GLIOBLASTOMA INVASION

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Neuro-Oncology 2015年第SUPPL_5期17卷 v59–v59页

作者： Martinez-Gutierrez, Juan Carlos Shah, Sagar R. Ruiz-Valls, Alejandro Wong, Bin S. Tippens, Nathaniel D. Konstantopoulos, Konstantinos Quinones-Hinojosa, Alfredo Johns Hopkins University School of Medicine Department of Neurosurgery Baltimore MD USA Johns Hopkins University School of Medicine Department of Biomedical Engineering Baltimore MD USA Johns Hopkins University Department of Chemical and Biomolecular Engineering Baltimore MD USA Department of Biological Statistics and Computational Biology Cornell UniversityDepartment of Biological Statistics and Computational Biology Cornell University Ithaca NY USA Tri-Institutional Training Program in Computational Biology and Medicine New York NY USA Johns Hopkins University School of Medicine Department of Oncology Baltimore MD USA

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Nitrogen and Phosphorus Removal from Wastewater Effluent Using Green Microalga Chlorella vulgaris: Theoretical understanding and model simulation 86

Nitrogen and Phosphorus Removal from Wastewater Effluent Usi...

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86th Annual Water Environment Federation Technical Exhibition and Conference, WEFTEC 2013

作者： Kim, Jinsoo Lee, Joo-Youp Lu, Ting Dunlap, Patrick Shaw, Andrew Barnard, James George, Biju Metz, Deborah Chemical Engineering Program Department of Biomedical Chemical and Environmental Engineering University of Cincinnati CincinnatiOH United States Black and Veatch United States Metropolitan Sewer District of Greater Cincinnati CincinnatiOH United States Greater Cincinnati Waterworks CincinnatiOH United States

ISBN: (纸本)9781510870444

Nutrient removal has become common in wastewater treatment with the goal of improving water quality in receiving streams. Traditional nutrient removal involves using selective nitrifying bacteria and excess oxygen, which consumes a significant amount of energy. Microalgae-derived biodiesel production has received great attention due to their ability to produce triacylglycerols for biodiesel while using solar energy and carbon dioxide (CO2). Based on experiment results, Y (gVSS/gN), μmax (h-1) and Ks (mg/L) were calculated for a Monod equation for algal growth and a process model was developed to compare an algae-based process to a tertiary nitrifying activated sludge process. Model simulations showed that the volume of an algae-based treatment reactor may need to be on the order of 40 times the size of a nitrifying activated sludge process, and that algae-based treatment produces almost 100 times the biomass compared with activated sludge for ammonia removal alone. Finally the paper summarizes the synergistic relationship between nutrient removal and algae growth in municipal wastewater treatment plants. Copyright © 2013 Water Environment Federation. All Rights Reserved.

关键词： Activated sludge process

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A Phosphorescent Molecular “Butterfly” that undergoes a Photoinduced Structural Change allowing Temperature Sensing and White Emission†

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Angewandte Chemie 2014年第41期126卷

作者： Mingu Han Yu Tian Zhao Yuan Lei Zhu Biwu Ma Chemical and Biomedical Engineering FAMU‐FSU College of Engineering(USA) Materials Science Program Florida State University (USA) Department of Chemistry and Biochemistry Florida State University (USA) http://bma.eng.fsu.edu

A butterfly‐like phosphorescent platinum(II) binuclear complex can undergo a molecular structure change in which the Pt–Pt distance shortens upon photoexcitation, which leads to the formation of two distinct excited states and dual emission in the steady state, that is, greenish‐blue emission from the high‐energy excited state at the long Pt–Pt distance and red emission from the low‐energy excited state at the short Pt–Pt distance. This photoinduced molecular structure change has a strong dependence on the molecule’s surrounding environment, allowing its application as self‐referenced luminescent sensor for solid–liquid phase change, viscosity, and temperature, with greenish‐blue emission in solid matrix and rising red emission in molten liquid phase. With proper control of the surrounding media to manipulate the structural change and photophysical properties, a broad white emission can be achieved from this molecular butterfly.

关键词： Duale Emission Metall‐Metall‐Wechselwirkungen Molekulare Sensoren Lichtinduzierte Umwandlungen Platinkomplexe

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Corrigendum to Cardiomyocyte differentiation of pluripotent stem cells with SB203580 analogues correlates with Wnt pathway CK1 inhibition independent of p38 MAPK signaling [J Mol Cell Cardiol 80 (2015) 56–70]

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Journal of Molecular and Cellular Cardiology 2015年 85卷 294-294页

作者： Filip Laco Joo-Leng Low Jasmin Seow Tsung Liang Woo Qixing Zhong Jayasree Seayad Zhenfeng Liu Heming Wei Shaul Reuveny David A. Elliott Christina L.L. Chai Steve K.W. Oh Bioprocessing Technology Institute 20 Biopolis Way Centros #06-01 Singapore 138668 Singapore Institute of Chemical and Engineering Sciences 8 Biomedical Grove Neuros #07-01 Singapore 138665 Singapore Department of Pharmacy Faculty of Science National University of Singapore 18 Science Drive 4 Singapore 117543 Singapore National Heart Research Institute Singapore National Heart Centre Singapore Singapore Cardiovascular & Metabolic Disorders Program DUKE-NUS Graduate Medical School Singapore Singapore Murdoch Childrens Research Institute The Royal Children's Hospital Flemington Road Parkville Melbourne Australia

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Correction: Discovery and characterization of functional modules and pathogenic genes associated with the risk of coronary artery disease

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RSC Advances 2015年第37期5卷 29304-29304页

作者： Wenna Nie Yana Lv Leyu Yan Tianbing Guan Qiang Li Xiaojuan Guo Wenhu Liu Mengpei Feng Guang Xu Xi Chen Haitao Lv Chongqing University Innovative Drug Research Centre School of Chemistry and Chemical Engineering Chongqing 401331 P. R. China Yunnan Branch Institute of Medicinal Plant Development Chinese Academy of Medical Sciences Peking Union Medical College Jinghong 666100 China Key Laboratory of Dai and Southern Medicine of Xishuangbanna Dai Autonomous Prefecture Jinghong 666100 China Institute of Medicinal Plant Development Chinese Academy of Medical Sciences Peking Union Medical College Beijing 100193 China Tissue Repair and Regeneration Program Institute of Health and Biomedical Innovation Queensland University of Technology Brisbane QLD 4059 Australia

Correction for ‘Discovery and characterization of functional modules and pathogenic genes associated with the risk of coronary artery disease’ by Wenna Nie et al., RSC Adv., 2015, 5, 26443–26451.

Correction for ‘Discovery and characterization of functional modules and pathogenic genes associated with the risk of coronary artery disease’ by Wenna Nie et al., RSC Adv., 2015, 5, 26443–26451.

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engineering Microorganisms for biomedical and Energy Applications

Engineering Microorganisms for Biomedical and Energy Applica...

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第八届全国微全分析系统学术会议、第三届全国微纳尺度生物分离分析学术会议暨第五届国际微化学与微系统学术会议

作者： I-Ming Hsing Danhui Cheng Department of Chemical and Biomolecular Engineering the Hong Kong University of Science and TechnologyHong Kong SARChina Division of Biomedical Engineering and Bioengineering Graduate Programthe Hong Kong University of Science and Technology Division of Biomedical Engineering and Bioengineering Graduate Program the Hong Kong University of Science and Technology

The availability of technologies to rapidly synthesize and decode genetic sequences has provided new applications for recombinant DNA technology and genetic engineering developed in early *** new genetic engineering approach,also coined as synthetic biology by many scientists,often involves engineering of microorganism through expression of heterologous gene segments in the microbial *** ability to engineer microorganisms empowered with tailor-made characteristics has provided exciting opportunities in different areas of *** instance,drug synthesis and novel drug delivery methods based on engineered bacteria strains have been extensively studied in biomedical ***,various synthetic biology approaches have been proposed to construct novel metabolic pathways in order to achieve higher yield in bioenergy & biofuel production,such as in ethanol fermentation,hydrogen production and bioelectricity generation through a microbial fuel *** this plenary presentation,we will start with an overview of this very exciting technology (*** biology),followed by a detailed discussion of two related projects in my *** the first part,we utilized yeast display technology for developing a new cell-based immunoassay *** the second one,we engineered photosynthetic bacteria to rewire its indigenous electron transfer pathway in pursuit of converting solar energy to ***,for the interests of our microfluidic community,some thoughts and opportunities of studying and utilizing engineered microorganisms in a microfluidic platform will be discussed.

关键词： Synthetic Biology Genetic engineering Yeast Surface Display Photosynthetic Bacteria

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Selective surface functionalization at regions of high local curvature in graphene

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chemical Communications 2013年第7期49卷 677-679页

作者： Wu, Qingzhi Wu, Yaping Hao, Yufeng Geng, Jianxin Charlton, Matthew Chen, Shanshan Ren, Yujie Ji, Hengxing Li, Huifeng Boukhvalov, Danil W. Piner, Richard D. Bielawski, Christopher W. Ruoff, Rodney S. State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Biomedical Materials and Engineering Center Wuhan University of Technology Wuhan 430070 China Department of Mechanical Engineering Department of Materials Science Department of Engineering Program The University of Texas at Austin Austin TX 78712 1 University Station C2200 United States Department of Chemistry and Biochemistry The University of Texas at Austin Austin TX 78712 1 University Station A1590 United States School of Computational Sciences Korea Institute for Advanced Study Seoul 130-722 South Korea ) School of Chemical and Biological Engineering Seoul National University Seoul 151-742 South Korea

Monolayer graphene was deposited on a Si wafer substrate decorated with SiO2 nanoparticles (NPs) and then exposed to aryl radicals that were generated in situ from their diazonium precursors. Using micro-Raman mapping, the aryl radicals were found to selectively react with the regions of graphene that covered the NPs. The enhanced chemical reactivity was attributed to the increased strain energy induced by the local mechanical deformation of the graphene. © The Royal Society of Chemistry.

关键词： GRAPHENE synthesis MONOMOLECULAR films SILICON wafers NANOPARTICLES -- Spectra DIAZONIUM compounds RAMAN effect

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