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Extracellular vesicle 2022年 1卷 100016页

作者： Sierra A Walker Irina Davidovich Yubo Yang Andrew Lai Jenifer Pendiuk Goncalves Vatsal Deliwala Sara Busatto Shane Shapiro Na'ama Koifman Carlos Salomon Yeshayahu Talmon Joy Wolfram Department of Biochemistry and Molecular Biology Department of Physiology and Biomedical Engineering Department of Transplantation Mayo Clinic Jacksonville FL 32224 USA. Department of Chemical Engineering and the Russell Berrie Nanotechnology Institute (RBNI) Technion-Israel Institute of Technology Haifa 3200003 Israel. Exosome Biology Laboratory Centre for Clinical Diagnostics University of Queensland Centre for Clinical Research Royal Brisbane and Women's Hospital Faculty of Medicine The University of Queensland Brisbane QLD 4006 Australia. Australian Institute for Bioengineering and Nanotechnology The University of Queensland Brisbane QLD 4072 Australia. School of Chemical Engineering The University of Queensland Brisbane QLD Australia. Vascular Biology Program Boston Children's Hospital Boston MA 02115 United States. Department of Surgery Harvard Medical School Boston MA 02115 United States. Department of Orthopedic Surgery Mayo Clinic Jacksonville FL 32224 USA. Center of Microscopy and Microanalysis The University of Queensland Brisbane QLD 4072 Australia. Departamento de Investigación Postgrado y Educación Continua (DIPEC) Facultad de Ciencias de la Salud Universidad del Alba Santiago 8320000 Chile.

Advancements in extracellular vesicle (EV) studies necessitate the development of optimized storage conditions to ensure preservation of physical and biochemical characteristics. In this study, the most common buffer for EV storage (phosphate-buffered saline/PBS) was compared to a cryoprotective 5% sucrose solution. The size distribution and concentration of EVs from two different sources changed to a greater extent after -80 °C storage in PBS compared to the sucrose solution. Additionally, molecular surface protrusions and transmembrane proteins were more prevalent in EVs stored in the sucrose solution compared to those stored in PBS. This study demonstrates, for the first time, that distinct ring-like molecular complexes and cristae-like folded membranous structures are visible upon EV degradation. Taken together, the size, concentration, molecular surface extensions, and transmembrane proteins of EVs varied substantially based on the buffer used for -80 °C storage, suggesting that biocompatible cryoprotectants, such as sucrose, should be considered for EV studies.

关键词： Cryoprotection exosome extracellular vesicle storage sucrose

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The applications of smart mobile device for detecting balance dysfunction in individuals with down syndrome

The applications of smart mobile device for detecting balanc...

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作者： Hsieh, Tsung-Hsun Peng, Chih-Wei Chen, Kai-Yun Huang, Ying-Zu Lin, Yi-Huang Zhong, Wei-Zong Liang, Jen-I Zhao, Jingpu Cheng, Chun-Yu Chang, Ya-Ju Cheng, Chih-Hsiu Chuang, Yu-Fen Healthy Aging Research Center School of Physical Therapy Graduate Institute of Rehabilitation Science Chang Gung University College of Medicine Taoyuan Taiwan Neuroscience Research Center Chang Gung Memorial Hospital Linkou Taoyuan Taiwan Department of Physical Medicine and Rehabilitation School of Medicine College of Medicine Taipei Medical University Taipei Taiwan School of Biomedical Engineering College of Biomedical Engineering Taipei Medical University Taipei Taiwan Graduate Institute of Neural Regenerative Medicine College of Medical Science and Technology Taipei Medical University Taipei Taiwan Department of Neurology Chang Gung Memorial Hospital College of Medicine Chang Gung University Taipei Taiwan Department of Rehabilitation Second People's Hospital of ShenZhen ShenZhen China Department of Physical Medicine and Rehabilitation Taipei Medical University Hospital Taipei Taiwan Department of Physical Medicine and Rehabilitation Chang Gung Memorial Hospital Linkou Taoyuan Taiwan

Falls are a major health concern leading cause of fatal and non-fatal injuries for neurological disorders. Balance dysfunction is one of the common factors to determine fall risk in neurological patients. Preventative measures may help to reduce the incidence and severity of falls for detecting balance function and fall risk factors. However, the objective measures for balance require expensive equipment with the assessment of clinical expertise. A main gap remains in the evaluation method to objectively characterize the balance functions in individuals with high risk of falling. With the development of wearable and mobile devices, recent advances in smart mobile devices may provide a potential opportunity to manage the gap in the detailed quantification of balance impairments. The purpose of this study is to identify whether the biomechanical data measured by the mobile device is reliable to characterize the posture stability in various balance test conditions. A total of 39 children with Down syndrome completed four balancetesting tasks under altered base of support and vision. Simultaneous biomechanical measurements were gathered from the iPod and force plate analysis system during functional balance testing. The force plate and mobile system provided similar patterns of stability across groups. Correlation (r2) between two systems for path length, 95% ellipse area, peakto- peak, standard deviation and mean ranged from 0.60 to 0.99. We expect that the smart mobile device can provide reliable and accurate information to quantify the postural stability in individuals with elderly people or neurological disorders. The objectivity, portability and easy use of such mobile device make it ideal to apply in clinical environments for detecting balance functions and reducing the risk of falls in Down syndrome or other neurological patients. © 2018 World Scientific Publishing Co. Pte Ltd. All rights reserved.

关键词： System stability

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Relationship between slip angle in ramp test and coefficient of friction values at shoe-floor interface measured with cart-type friction measurement device

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Journal of Biomechanical Science and engineering 2018年第1期13卷 17-00389-17-00389页

作者： Yamaguchi, Takeshi Yamada, Ryo Warita, Iori Shibata, Kei Ohnishi, Akihito Sugama, Atsushi Hinoshita, Mamoru Sakauchi, Koichi Matsukawa, Seiko Hokkirigawa, Kazuo Graduate School of Engineering Tohoku University Japan Graduate School of Biomedical Engineering Tohoku University Aramaki-Aza-Aoba 6-6-01 Aoba-ku Sendai Miyagi980-8579 Japan Umezono 1-4-6 Kiyose Tokyo204-0024 Japan C.I. TAKIRON Corporation Japan Nagano 405 Yasutomi-cho Himeji Hyogo671-2421 Japan

The cart-type friction measurement device developed by the authors facilitates measurement of both the static coefficient of friction (SCOF) and the dynamic coefficient of friction (DCOF) between the shoe and the floor simultaneously, as well as measurement with variation in sliding velocity. However, whether slip-resistance evaluation using this cart-type friction measurement device corresponds to the actual slip and fall risks is unclear. To investigate the validity of evaluation of slip resistance between the shoe and the floor by using the SCOF and DCOF values measured with a cart-type friction measurement device, we aimed to investigate the relationship between the slip angle in a ramp test and the coefficient of friction (COF) values between the test safety shoe and the 10 test floor sheets contaminated with a glycerol solution. The results indicate that the SCOF values and the DCOF values corresponding to sliding velocity lower than 0.3 m/s are highly correlated with the slip angle in the ramp test, which suggests that the cart-type friction measurement device can simulate the slip between the shoe and the floor in the ramp test under such sliding velocity conditions. Because the ramp test has been used widely to assess the slip resistance of floors and because the slip angle is highly correlated to the risk of slip-induced falls during level walking, the results suggest that the cart-type friction measurement device is valid and effective for assessing the slip resistance between the shoe and the floor. This study provides new information about the evaluation of slip resistance and indicates that the cart-type friction measurement device will contribute toward the prevention of slip-induced fall accidents. © 2018 The Japan Society of Mechanical Engineers.

关键词： Floors

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Rapid bacterial detection using a microfluidic system integrating membrane filtration and surface-enhanced Raman scattering 22

Rapid bacterial detection using a microfluidic system integr...

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22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018

作者： Chang, Kai-Wei Cheng, Ho-Wen Shiue, Jessie Wang, Juan-Kai Wang, Yuh-Lin Huang, Nien-Tsu Graduate Institute of Biomedical Electronics and Bioinformatics National Taiwan University Taiwan Institute of Atomic and Molecular Sciences Academia Sinica Taiwan Institute of Physics Academia Sinica Taiwan Center for Condensed Matter Science National Taiwan University Taiwan Center for Atomic Initiative for New Materials National Taiwan University Taiwan Department of Physics National Taiwan University Taiwan Department of Electrical Engineering National Taiwan University Taiwan

ISBN: (纸本)9781510897571

A bacterial detection method has been developed using a microfluidic system integrating membrane filtration and surface-enhanced Raman scattering (SERS). Based on its highly-sensitive and label-free features, SERS has been reported for detecting various bacteria by analyzing their secreted metabolites. However, a prolonged (>16 hours) culturing time is usually required due to the low concentration of bacteria (1-100 CFU/mL) in patients’ biological samples. To address this problem and simplify the sample pre-treatment process, we demonstrate a microfluidic system with membrane filtration and SERS detection to achieve high throughput (~1 mL/min) bacterial enrichment and real-time, label-free bacterial detection. Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved.

关键词： Bacteria

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Near-Infrared Light Increases Functional Connectivity with a Non-thermal Mechanism

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Cerebral cortex communications 2020年第1期1卷 tgaa004页

作者： Grzegorz M Dmochowski Ahmed Duke Shereen Destiny Berisha Jacek P Dmochowski Princess Margaret Cancer Centre Toronto ON M5G 1L7 Canada. Advanced Science Research Center Graduate Center of the City University of New York New York NY 10031 USA. Department of Biomedical Engineering City College of New York New York NY 10031 USA.

Although techniques for noninvasive brain stimulation are under intense investigation, an approach that has received limited attention is transcranial photobiomodulation (tPBM), the delivery of near-infrared light to the brain with a laser or light-emitting diode directed at the scalp. Here we employed functional magnetic resonance imaging to measure the blood-oxygenation-level-dependent signal in = 20 healthy human participants while concurrently stimulating their right frontal pole with a near-infrared laser. Functional connectivity with the illuminated region increased by up to 15% during stimulation, with a quarter of all connections experiencing a significant increase. The time course of connectivity exhibited a sharp rise approximately 1 min after illumination onset. Brain-wide connectivity increases were also observed, with connections involving the stimulated hemisphere showing a significantly larger increase than those in the contralateral hemisphere. We subsequently employed magnetic resonance thermometry to measure brain temperature during tPBM (separate cohort, = 20) and found no significant temperature differences between active and sham stimulation. Our findings suggest that near-infrared light synchronizes brain activity with a nonthermal mechanism, underscoring the promise of tPBM as a new technique for stimulating brain function.

关键词： fMRI functional connectivity low-level laser therapy neuromodulation photobiomodulation

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Modeling Alzheimer's disease progression using deep recurrent neural networks

Modeling Alzheimer's disease progression using deep recurren...

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2018 International Workshop on Pattern Recognition in Neuroimaging, PRNI 2018

作者： Nguyen, Minh Sun, Nanbo Alexander, Daniel C. Feng, Jiashi Thomas Yeo, B.T. Clinical Imaging Research Centre Singapore Institute for Neurotechnology National University of Singapore Singapore Singapore Department of Electrical and Computer Engineering National University of Singapore Singapore Singapore Centre for Medical Image Computing Department of Computer Science University College London London United Kingdom Martinos Center for Biomedical Imaging Massachusetts General Hospital CharlestownMA United States Centre for Cognitive Neuroscience Duke-NUS Medical School Singapore Singapore NUS Graduate School for Integrative Sciences and Engineering National University of Singapore Singapore Singapore

ISBN: (纸本)9781538668597

Multi-step prediction, predicting values multiple steps ahead, is a difficult problem because of the accumulation of error after successive predictions. Recurrent neural networks (RNN) have been applied successfully to multi-step prediction problems such as language modeling where training data are abundant and regular. However, applying RNN to problems in healthcare is still challenging as RNN requires large, feature complete training data in which intervals between time points are fixed which are not reflective of healthcare data. To overcome these problems, we propose strategies to train RNN using missing data which involve using the RNN to fill in the incomplete or missing time points online. The strategies enables the efficient use of training data as well as the adoption of RNN in problems where the variables in a multivariate time series are recorded at irregular intervals or at different frequencies, where there are a lot of missing data. We train RNN using the Alzheimer's Disease Neuroimaging Initiative (ADNI) database to model the monthly progression of Alzheimer's disease for seven years into the future which is a multi-step prediction problem. We show that RNN outperforms baseline models. © 2018 IEEE.

关键词： Recurrent neural networks

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Metatranscriptomics uncovers diurnal functional shifts in bacterial transgenes with profound metabolic effects

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Cell Host & Microbe 2025年

作者： Stephany Flores Ramos Nicole Siguenza Wuling Zhong Ipsita Mohanty Amulya Lingaraju R. Alexander Richter Smruthi Karthikeyan April L. Lukowski Qiyun Zhu Wilhan D.G. Nunes Jasmine Zemlin Zhenjiang Zech Xu Jeff Hasty Pieter C. Dorrestein Satchidananda Panda Rob Knight Amir Zarrinpar Division of Gastroenterology University of California San Diego La Jolla CA USA Biomedical Sciences Graduate Program University of California San Diego La Jolla CA USA Department of Pediatrics University of California San Diego La Jolla CA USA Skaggs School of Pharmacy and Pharmaceutical Sciences University of California San Diego La Jolla CA USA Division of Engineering and Applied Science California Institute of Technology Pasadena CA USA Scripps Institution of Oceanography University of California San Diego La Jolla CA USA School of Life Sciences Arizona State University Tempe AZ USA Biodesign Center for Fundamental and Applied Microbiomics Arizona State University Tempe AZ USA Collaborative Mass Spectrometry Innovation Center Skaggs School of Pharmacy and Pharmaceutical Sciences University of California San Diego La Jolla CA USA Key Laboratory of Food Science and Technology Nanchang University Nanchang China Shu Chien-Gene Lay Department of Bioengineering University of California San Diego La Jolla CA USA Center for Microbiome Innovation University of California San Diego La Jolla CA USA Synthetic Biology Institute University of California San Diego La Jolla CA USA Salk Institute for Biological Studies La Jolla CA USA Department of Computer Science and Engineering University of California San Diego La Jolla CA USA Halıcıoğlu Data Science Institute University of California San Diego La Jolla CA USA Moores Cancer Center University of California San Diego La Jolla CA USA Division of Gastroenterology Jennifer Moreno Department of Veterans Affairs Medical Center La Jolla CA USA Institute of Diabetes and Metabolic Health University of California San Diego La Jolla CA USA

Diurnal rhythmicity in the gut maintains gut integrity, circadian rhythms, and metabolic homeostasis. However, existing studies focus on microbial composition rather than transcriptional activity. To understand microbial functional dynamics, we characterize diurnal fluctuations in the mouse cecal metatranscriptome and metagenome under high-fat diet and time-restricted feeding (TRF). We show that metatranscriptomics uncovers TRF-induced time-dependent microbial functional shifts that are undetectable with metagenomics alone. We also found bile salt hydrolase ( bsh ) from Dubosiella newyorkensis exhibits diurnal expression in the TRF group. engineering this bsh , along with other candidates, into a native E. coli chassis reveals distinct differences in deconjugation and amidation activities, underscoring functional specificity. In vivo , a D. newyorkensis bsh improves insulin sensitivity, glucose tolerance, and body composition, suggesting a direct role in TRF metabolic benefits. This study highlights how coupling metatranscriptomics with engineered bacterial systems is a powerful approach for uncovering time-dependent bacterial functions related to health and disease.

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Neoantigen-augmented iPSC cancer vaccine combined with radiotherapy promotes antitumor immunity in poorly immunogenic cancers

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NPJ vaccines 2024年第1期9卷 95页

作者： Kevin Chih-Yang Huang William Tzu-Liang Chen Jia-Yi Chen Chien-Yueh Lee Chia-Hsin Wu Chia-Ying Lai Pei-Chen Yang Ji-An Liang An-Cheng Shiau K S Clifford Chao Tao-Wei Ke Department of Biomedical Imaging and Radiological Science China Medical University Taichung 406040 Taiwan ROC. chihyang0425@mail.cmu.edu.tw. Translation Research Core China Medical University Hospital China Medical University Taichung 404327 Taiwan ROC. chihyang0425@mail.cmu.edu.tw. Cancer Biology and Precision Therapeutics Center China Medical University Taichung 406040 Taiwan ROC. chihyang0425@mail.cmu.edu.tw. Department of Surgery School of Medicine China Medical University Taichung 406040 Taiwan ROC. Department of Colorectal Surgery China Medical University HsinChu Hospital China Medical University HsinChu 302 Taiwan ROC. Department of Colorectal Surgery China Medical University Hospital China Medical University Taichung 404327 Taiwan ROC. Proton Therapy and Science Center China Medical University Hospital China Medical University Taichung 404327 Taiwan ROC. Innovation Frontier Institute of Research for Science and Technology National Taipei University of Technology Taipei 106344 Taiwan ROC. Department of Electrical Engineering National Taipei University of Technology Taipei 106344 Taiwan ROC. Department of Biomedical Engineering China Medical University Taichung 406040 Taiwan ROC. Bioinformatics and Biostatistics Core Centers of Genomic and Precision Medicine National Taiwan University Taipei 10055 Taiwan ROC. Department of Biomedical Imaging and Radiological Science China Medical University Taichung 406040 Taiwan ROC. Department of Radiation Oncology China Medical University Hospital China Medical University Taichung 404327 Taiwan ROC. Department of Radiotherapy School of Medicine China Medical University Taichung 406040 Taiwan ROC. Proton Therapy and Science Center China Medical University Hospital China Medical University Taichung 404327 Taiwan ROC. d94032@mail.cmuh.org.tw. Department of Radiation Oncology China Medical University Hospital China Medical University Taichung 404327 Taiwan ROC. d94032@mail.cmuh.org.tw.

Although irradiated induced-pluripotent stem cells (iPSCs) as a prophylactic cancer vaccine elicit an antitumor immune response, the therapeutic efficacy of iPSC-based cancer vaccines is not promising due to their insufficient antigenicity and the immunosuppressive tumor microenvironment. Here, we found that neoantigen-engineered iPSC cancer vaccines can trigger neoantigen-specific T cell responses to eradicate cancer cells and increase the therapeutic efficacy of RT in poorly immunogenic colorectal cancer (CRC) and triple-negative breast cancer (TNBC). We generated neoantigen-augmented iPSCs (NA-iPSCs) by engineering AAV2 vector carrying murine neoantigens and evaluated their therapeutic efficacy in combination with radiotherapy. After administration of NA-iPSC cancer vaccine and radiotherapy, we found that ~60% of tumor-bearing mice achieved a complete response in microsatellite-stable CRC model. Furthermore, splenocytes from mice treated with NA-iPSC plus RT produced high levels of IFNγ secretion in response to neoantigens and had a greater cytotoxicity to cancer cells, suggesting that the NA-iPSC vaccine combined with radiotherapy elicited a superior neoantigen-specific T-cell response to eradicate cancer cells. The superior therapeutic efficacy of NA-iPSCs engineered by mouse TNBC neoantigens was also observed in the syngeneic immunocompetent TNBC mouse model. We found that the risk of spontaneous lung and liver metastasis was dramatically decreased by NA-iPSCs plus RT in the TNBC animal model. Altogether, these results indicated that autologous iPSC cancer vaccines engineered by neoantigens can elicit a high neoantigen-specific T-cell response, promote tumor regression, and reduce the risk of distant metastasis in combination with local radiotherapy.

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Corrigendum to Functionalized collagen-silver nanocomposites for evaluation of the biocompatibility and vascular differentiation capacity of mesenchymal stem cells Volume 624 (2021), 126814

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Colloids and Surfaces A: Physicochemical and engineering Aspects 2022年 648卷

作者： Kai-Bo Chang Chiung-Chyi Shen Shan-hui Hsu Chang Ming Tang Yi-Chin Yang Sin-Ying Liu Tian Ren Ku Mei-Lang Kung Hsien-Hsu Hsieh Huey-Shan Hung Graduate Institute of Biomedical Science China Medical University Taichung 40402 Taiwan ROC Neurological Institute Head of Department of Neurosurgery Taichung Veterans General Hospital Taichung 407204 Taiwan ROC Department of Physical Therapy Hung Kuang University Taichung Taiwan ROC Basic Medical Education Center Central Taiwan University of Science and Technology Taichung 406053 Taiwan ROC Institute of Polymer Science and Engineering National Taiwan University Taipei 10617 Taiwan ROC Collage of Oral Medicine Chung Shan Medical University Taichung 55015 Taiwan ROC Department of Medical Education and Research Kaohsiung Veterans General Hospital Kaohsiung 43302 Taiwan ROC Blood Bank Taichung Veterans General Hospital Taichung 407204 Taiwan ROC Translational Medicine Research China Medical University Hospital Taichung 40402 Taiwan ROC

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Guidelines and definitions for research on epithelial-mesenchymal transition (vol 21, pg 341, 2020)

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NATURE REVIEWS MOLECULAR CELL BIOLOGY 2021年第12期22卷 834-834页

作者： Yang, Jing Antin, Parker Berx, Geert Blanpain, Cedric Brabletz, Thomas Bronner, Marianne Campbell, Kyra Cano, Amparo Casanova, Jordi Christofori, Gerhard Dedhar, Shoukat Derynck, Rik Ford, Heide L. Fuxe, Jonas Garcia de Herreros, Antonio Goodall, Gregory J. Hadjantonakis, Anna-Katerina Huang, Ruby Y. J. Kalcheim, Chaya Kalluri, Raghu Kang, Yibin Khew-Goodall, Yeesim Levine, Herbert Liu, Jinsong Longmore, Gregory D. Mani, Sendurai A. Massague, Joan Mayor, Roberto McClay, David Mostov, Keith E. Newgreen, Donald F. Nieto, M. Angela Puisieux, Alain Runyan, Raymond Savagner, Pierre Stanger, Ben Stemmler, Marc P. Takahashi, Yoshiko Takeichi, Masatoshi Theveneau, Eric Thiery, Jean Paul Thompson, Erik W. Weinberg, Robert A. Williams, Elizabeth D. Xing, Jianhua Zhou, Binhua P. Sheng, Guojun Departments of Pharmacology and Pediatrics Moores Cancer Center University of California San Diego La Jolla CA USA Department of Cellular and Molecular Medicine University of Arizona Tucson AZ USA Molecular and Cellular Oncology Lab Department of Biomedical Molecular Biology Ghent University Cancer Research Institute Ghent (CRIG) VIB Center for Inflammation Research Ghent Belgium Laboratory of Stem Cells and Cancer Université Libre de Bruxelles Bruxelles Belgium Department of Experimental Medicine 1 Nikolaus-Fiebiger-Center for Molecular Medicine Friedrich-Alexander-University Erlangen-Nürnberg Erlangen Germany Division of Biology and Biological Engineering California Institute of Technology Pasadena CA USA Department of Biomedical Science and Bateson Centre University of Sheffield Sheffield UK Departamento de Bioquímica Universidad Autónoma de Madrid (UAM) Instituto de Investigaciones Biomédicas ‘Alberto Sols’ (CSIC-UAM) IdiPAZ & Centro de Investigación Biomédica en Red de Cáncer (CIBERONC) Madrid Spain Institute for Research in Biomedicine (IRB Barcelona) Barcelona Institute of Science and Technology/Institut de Biologia Molecular de Barcelona (IBMB-CSIC) Barcelona Spain Department of Biomedicine University of Basel Basel Switzerland Department of Biochemistry and Molecular Biology University of British Columbia and British Columbia Cancer Research Centre Vancouver BC Canada Departments of Cell and Tissue Biology and Anatomy University of California at San Francisco San Francisco CA USA Department of Pharmacology University of Colorado Anschutz Medical Campus Aurora CO USA Department of Laboratory Medicine (LABMED) Division of Pathology Karolinska University Hospital and Department of Microbiology Tumor and Cell Biology (MTC) Karolinska Institutet Stockholm Sweden Programa de Recerca en Càncer Institut Hospital del Mar d’Investigacions Mèdiques (IMIM) and Departament de Ciències Experimentals i de la Salut Universitat Pompeu Fabra Barcelona Spain C

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