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Molecular Basis for Two Stereoselective Diels–Alderases that Produce Decalin Skeletons**

Angewandte Chemie 2021年第41期133卷

作者： Dr. Keisuke Fujiyama Dr. Naoki Kato Dr. Suyong Re Kiyomi Kinugasa Dr. Kohei Watanabe Dr. Ryo Takita Dr. Toshihiko Nogawa Dr. Tomoya Hino Dr. Hiroyuki Osada Dr. Yuji Sugita Dr. Shunji Takahashi Prof. Shingo Nagano Department of Chemistry and Biotechnology Graduate School of Engineering Tottori University 4-101 Koyama-cho Minami Tottori 680-8552 Japan Current address: Dormancy and Adaptation Research Unit RIKEN Center for Sustainable Resource Science 1-7-22 Suehiro Tsurumi Yokohama Kanagawa 230-0045 Japan Natural Product Biosynthesis Research Unit RIKEN Center for Sustainable Research Science 2-1 Hirosawa Wako Saitama 351-0198 Japan Faculty of Agriculture Setsunan University 45-1 Nagaotoge-cho Hirakata Osaka 573-0101 Japan Laboratory for Biomolecular Function Simulation RIKEN Center for Biosystems Dynamics Research 2-2-3 Minatojima-minami-machi Chuo-ku Kobe Hyogo 650-0047 Japan Artificial Intelligence Center for Health and Biomedical Research National Institutes of Biomedical Innovation Health and Nutrition 7-6-8 Saito-Asagi Ibaraki Osaka 567-0085 Japan Graduate School of Pharmaceutical Sciences The University of Tokyo 7-3-1 Hongo Bunkyo-ku Tokyo 113-0033 Japan Chemical Biology Research Group RIKEN Center for Sustainable Research Science 2-1 Hirosawa Wako Saitama 351-0198 Japan Center for Research on Green Sustainable Chemistry Tottori University 4-101 Koyama-cho Minami Tottori 680-8552 Japan Theoretical Molecular Science Laboratory RIKEN Cluster for Pioneering Research 2-1 Hirosawa Wako Saitama 351-0198 Japan Computational Biophysics Research Team RIKEN Center for Computational Science 7-1-26 Minatojima-minami-machi Chuo-ku Kobe Hyogo 650-0047 Japan

Enzymes catalyzing [4+2] cycloaddition have attracted increasing attention because of their key roles in natural product biosynthesis. Here, we solved the X-ray crystal structures of a pair of decalin synthases, Fsa2 and Phm7, that catalyze intramolecular [4+2] cycloadditions to form enantiomeric decalin scaffolds during biosynthesis of the HIV-1 integrase inhibitor equisetin and its stereochemical opposite, phomasetin. Computational modeling, using molecular dynamics simulations as well as quantum chemical calculations, demonstrates that the reactions proceed through synergetic conformational constraints assuring transition state-like substrates folds and their stabilization by specific protein-substrate interactions. Site-directed mutagenesis experiments verified the binding models. Intriguingly, the flexibility of bound substrates is largely different in two enzymes, suggesting the distinctive mechanism of dynamics regulation behind these stereoselective reactions. The proposed reaction mechanism herein deepens the basic understanding how these enzymes work but also provides a guiding principle to create artificial enzymes.

关键词： [4+2] cycloaddition biosynthesis molecular dynamics natural products stereoselectivity

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Neural Recording: High‐Performance Graphene‐Fiber‐Based Neural Recording Microelectrodes (Adv. Mater. 15/2019)

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Advanced Materials 2019年第15期31卷

作者： Kezhong Wang Christopher L. Frewin Dorna Esrafilzadeh Changchun Yu Caiyun Wang Joseph J. Pancrazio Mario Romero‐Ortega Rouhollah Jalili Gordon Wallace Intelligent Polymer Research Institute ARC Centre of Excellence for Electromaterials Science University of Wollongong Wollongong NSW 2522 Australia Department of Bioengineering University of Texas at Dallas 800 W. Campbell Road Richardson TX 75080 USA Graduate School of Biomedical Engineering University of New South Wales Sydney NSW 2031 Australia School of Chemical Engineering University of New South Wales Sydney NSW 2031 Australia

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Inside Cover: Molecular Basis for Two Stereoselective Diels–Alderases that Produce Decalin Skeletons (Angew. Chem. Int. Ed. 41/2021)

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Angewandte Chemie International Edition 2021年第41期60卷

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Quality of life after total vs distal gastrectomy with Rouxen-Y reconstruction: Use of the Postgastrectomy Syndrome Assessment Scale-45

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World Journal of Gastroenterology 2017年第11期23卷 2068-2076页

作者： Masazumi Takahashi Masanori Terashima Hiroshi Kawahira Eishi Nagai Yoshikazu Uenosono Shinichi Kinami Yasuhiro Nagata Masashi Yoshida Keishiro Aoyagi Yasuhiro Kodera Koji Nakada Division of Gastroenterological Surgery Yokohama Municipal Citizen’s Hospital Division of Gastric Surgery Shizuoka Cancer Center Center for Frontier Medical Engineering Chiba University Department of Surgery and Science Graduate school of medicine Kyushu University Department of Digestive Surgery Kagoshima University Graduate School of Medicine Department of Surgical Oncology Kanazawa Medical School Center for Comprehensive Community Care Education Graduate School of Biomedical Sciences Nagasaki University Department of Surgery International University of Health and Welfare Hospital Department of Surgery Kurume University School of Medicine Department of Gastroenterological Surgery Nagoya University Graduate School of Medicine Department of Laboratory Medicine The Jikei University School of Medicine

AIM To investigate the detrimental impact of loss of reservoir capacity by comparing total gastrectomy(TGRY) and distal gastrectomy with the same Rouxen-Y(DGRY) reconstruction. The study was conducted using an integrated questionnaire, the Postgastrectomy Syndrome Assessment Scale(PGSAS)-45, recently developed by the Japan Postgastrectomy Syndrome Working *** The PGSAS-45 comprises 8 items from the Short Form-8, 15 from the Gastrointestinal Symptom Rating Scale, and 22 newly selected items. Uni-and multivariate analysis was performed on 868 questionnaires completed by patients who underwent either TGRY(n = 393) or DGRY(n = 475) for stage I gastric cancer(52 institutions). Multivariate analysis weighed of six explanatory variables, including the type of gastrectomy(TGRY/DGRY), interval after surgery, age, gender, surgical approach(laparoscopic/open), and whether the celiac branch of the vagus nerve was preserved/divided on the quality of life(QOL).RESULTS The patients who underwent TGRY experienced the poorer QOL compared to DGRY in the 15 of 19 main outcome measures of PGSAS-45. Moreover, multiple regression analysis indicated that the type of gastrectomy, TGRY, most strongly and broadly impaired the postoperative QOL among six explanatory variables. CONCLUSION The results of the present study suggested that TGRY had a certain detrimental impact on the postoperative QOL, and the loss of reservoir capacity could be a major cause.

关键词： Postgastrectomy syndrome Quality of life Gastric cancer Gastrectomy Patient-reported outcome

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Correction to: Decision Tree Based Classification of Abdominal Aortic Aneurysms Using Geometry Quantification Measures

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Annals of biomedical engineering 2019年第1期47卷 332页

作者： Shalin A Parikh Raymond Gomez Mirunalini Thirugnanasambandam Sathyajeeth S Chauhan Victor De Oliveira Satish C Muluk Mark K Eskandari Ender A Finol UTSA/UTHSA Joint Graduate Program in Biomedical Engineering University of Texas at San Antonio One UTSA Circle San Antonio TX 78249 USA. Department of Mechanical Engineering University of Texas at San Antonio One UTSA Circle San Antonio TX 78249 USA. Department of Management Science and Statistics University of Texas at San Antonio One UTSA Circle San Antonio TX 78249 USA. Department of Thoracic & Cardiovascular Surgery Allegheny Health Network Allegheny General Hospital 320 E. North Ave. Pittsburgh PA 15212 USA. Division of Vascular Surgery and Department of Radiology Northwestern University Feinberg School of Medicine 420 E. Superior St. Chicago IL 60611 USA. UTSA/UTHSA Joint Graduate Program in Biomedical Engineering University of Texas at San Antonio One UTSA Circle San Antonio TX 78249 USA. ender.finol@utsa.edu. Department of Mechanical Engineering University of Texas at San Antonio One UTSA Circle San Antonio TX 78249 USA. ender.finol@utsa.edu.

This erratum is to correct the variable name on the left hand side of Eq. (2). The correct variable name is "Diameter" rather than the stated "Area."

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Correction: Complement activation by gold nanoparticles passivated with polyelectrolyte ligands

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RSC advances 2018年第15期8卷 8247页

作者： Quang Huy Quach James Chen Yong Kah Department of Biomedical Engineering Faculty of Engineering National University of Singapore Singapore 117583 Singapore. NUS Graduate School for Integrative Science and Engineering National University of Singapore Singapore biekahj@nus.edu.sg.

[This corrects the article DOI: 10.1039/C7RA13325A.].

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CLEM of Neurons, Tissues and Biofilms immersed in Liquid using The Atmospheric Scanning Electron Microscope (ASEM): Dual Gold-Labeling

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Microscopy and Microanalysis 2018年第S1期24卷 340-341页

作者： Chikara Sato Nassir Memtily Mari Sato Toshiko Yamazawa Takaaki Kinoshita Shoko Nishihara Shinya Sugimoto Biomedical Research Institute National Institute of Industrial Science and Technology (AIST) Tsukuba 305-8566 Japan. Department of Molecular Physiology The Jikei University School of Medicine Tokyo 1058461 Japan. Laboratory of Cell Biology Department of Bioinformatics Graduate School of Engineering Soka University 1-236 Tangi-machi Hachioji Tokyo 192-8577 Japan. Department of Bacteriology The Jikei University School of Medicine Tokyo 1058461 Japan.

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FinnGen provides genetic insights from a well-phenotyped isolated population (vol 613, pg 508, 2023)

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NATURE 2023年第7952期615卷 E19-E19页

作者： Kurki, Mitja I. Karjalainen, Juha Palta, Priit Sipila, Timo P. Kristiansson, Kati Donner, Kati M. Reeve, Mary P. Laivuori, Hannele Aavikko, Mervi Kaunisto, Mari A. Loukola, Anu Lahtela, Elisa Mattsson, Hannele Laiho, Paivi Parolo, Pietro Della Briotta Lehisto, Arto A. Kanai, Masahiro Mars, Nina Ramo, Joel Kiiskinen, Tuomo Heyne, Henrike O. Veerapen, Kumar Rueger, Sina Lemmela, Susanna Zhou, Wei Ruotsalainen, Sanni Parn, Kalle Hiekkalinna, Tero Koskelainen, Sami Paajanen, Teemu Llorens, Vincent Gracia-Tabuenca, Javier Siirtola, Harri Reis, Kadri Elnahas, Abdelrahman G. Sun, Benjamin Foley, Christopher N. Aalto-Setala, Katriina Alasoo, Kaur Arvas, Mikko Auro, Kirsi Biswas, Shameek Bizaki-Vallaskangas, Argyro Carpen, Olli Chen, Chia-Yen Dada, Oluwaseun A. Ding, Zhihao Ehm, Margaret G. Eklund, Kari Farkkila, Martti Finucane, Hilary Ganna, Andrea Ghazal, Awaisa Graham, Robert R. Green, Eric M. Hakanen, Antti Hautalahti, Marco Hedman, Asa K. Hiltunen, Mikko Hinttala, Reetta Hovatta, Iiris Hu, Xinli Huertas-Vazquez, Adriana Huilaja, Laura Hunkapiller, Julie Jacob, Howard Jensen, Jan-Nygaard Joensuu, Heikki John, Sally Julkunen, Valtteri Jung, Marc Junttila, Juhani Kaarniranta, Kai Kahonen, Mika Kajanne, Risto Kallio, Lila Kalviainen, Reetta Kaprio, Jaakko Kerimov, Nurlan Kettunen, Johannes Kilpelainen, Elina Kilpi, Terhi Klinger, Katherine Kosma, Veli-Matti Kuopio, Teijo Kurra, Venla Laisk, Triin Laukkanen, Jari Lawless, Nathan Liu, Aoxing Longerich, Simonne Magi, Reedik Makela, Johanna Makitie, Antti Malarstig, Anders Mannermaa, Arto Maranville, Joseph Matakidou, Athena Meretoja, Tuomo Mozaffari, Sahar V. Niemi, Mari E. K. Niemi, Marianna Niiranen, Teemu O'Donnell, Christopher J. Obeidat, Ma'En Okafo, George Ollila, Hanna M. Palomaki, Antti Palotie, Tuula Partanen, Jukka Paul, Dirk S. Pelkonen, Margit Pendergrass, Rion K. Petrovski, Slave Pitkaranta, Anne Platt, Adam Pulford, David Punkka, Eero Pussinen, Pirkko Raghavan, Neha Rahimov, Fedik Rajpal, Deepak Renaud, Nicole A. Riley-Gillis, Bridget Rodosthenous, Rodostheni Institute for Molecular Medicine Finland (FIMM) Helsinki Institute of Life Science (HiLIFE) University of Helsinki Helsinki Finland Program in Medical and Population Genetics Broad Institute of Harvard and MIT Cambridge MA USA Stanley Center for Psychiatric Research Broad Institute of Harvard and MIT Cambridge MA USA Analytic and Translational Genetics Unit Massachusetts General Hospital Boston MA USA Estonian Genome Centre Institute of Genomics University of Tartu Tartu Estonia Medical and Clinical Genetics University of Helsinki and Helsinki University Hospital Helsinki Finland Department of Obstetrics and Gynecology Tampere University Hospital Tampere Finland Faculty of Medicine and Health Technology Center for Child Adolescent and Maternal Health University of Tampere Tampere Finland Department of Biomedical Informatics Harvard Medical School Boston MA USA Digital Health Center Hasso Plattner Institute for Digital Engineering University of Potsdam Potsdam Potsdam Germany Hasso Plattner Institute for Digital Health at Mount Sinai Department of Genetics and Genomic Sciences Icahn School of Medicine at Mount Sinai New York NY USA Finnish Institute for Health and Welfare (THL) Helsinki Finland Department of Public Health University of Helsinki Helsinki Finland Anesthesia Critical Care and Pain Medicine Massachusetts General Hospital Boston MA USA Abdominal Center Endocrinology Helsinki University Hospital Helsinki Finland Folkhalsan Research Center Helsinki Finland Research Program of Clinical and Molecular Metabolism University of Helsinki Helsinki Finland Biocenter Oulu University of Oulu Oulu Finland Computational Medicine Center for Life Course Health Research Faculty of Medicine University of Oulu Oulu Finland Turku University Hospital and University of Turku Turku Finland University of Turku Turku Finland Helsinki Biobank University of Helsinki and Hospital District of Helsinki and Uusimaa Helsinki Finland TAUCHI Research Center

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Molecular landscape of sex- and modality-specific exercise adaptation in human skeletal muscle through large-scale multi-omics integration

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Cell Reports 2025年第6期44卷 115750页

作者： Jacques, Macsue Landen, Shanie Sharples, Adam P. Garnham, Andrew Schittenhelm, Ralf Steele, Joel Heikkinen, Aino Sillanpää, Elina Ollikainen, Miina Broatch, James Zarekookandeh, Navabeh Hanson, Ola Ekström, Ola Asplund, Olof Lamon, Séverine Alexander, Sarah E. Smith, Cassandra Bauer, Carlie Woessner, Mary N. Levinger, Itamar Teschendorff, Andrew E. Gillberg, Linn Blom, Ida Helge, Jørn Wulff Harvey, Nicholas R. Haupt, Larisa M. Griffiths, Lyn R. Deshmukh, Atul S. Pietiläinen, Kirsi H. Piirilä, Päivi Seaborne, Robert A.E. Klevjer, Marie Bye, Anja Wisløff, Ulrik Jones-Freeman, Bernadette Eynon, Nir Australian Regenerative Medicine Institute Monash University Clayton VIC Australia Institute for Health and Sport (iHeS) Victoria University Footscray VIC Australia Centre for Endocrinology and Metabolism Hudson Institute of Medical Research Melbourne VIC Australia Institute of Physical Performance Norwegian School of Sport Sciences Oslo Norway Monash Proteomics & Metabolomics Platform Monash Biomedicine Discovery Institute Monash University Melbourne VIC Australia Institute for Molecular Medicine Finland (FIMM) Helsinki University Helsinki Finland Faculty of Sport and Health Sciences University of Jyväskylä Jyväskylä Finland Wellbeing Services County of Central Finland Jyväskylä Finland Minerva Foundation Institute for Medical Research Helsinki Finland Department of Clinical Sciences Translational Muscle Research Unit Lund University Diabetes Center Lund University Lund Sweden Institute for Physical Activity and Nutrition (IPAN) School of Exercise and Nutrition Sciences Deakin University 221 Burwood Highway Burwood 3125 VIC Australia Cardiometabolic Health and Exercise Physiology Laboratory Baker Heart and Diabetes Institute 75 Commercial Road Melbourne 3004 VIC Australia Nutrition & Health Innovation Research Institute Edith Cowan University Joondalup WA Australia Medical School The University of Western Australia Perth WA Australia Australian Institute for Musculoskeletal Science (AIMSS) Victoria University Melbourne VIC Australia CAS Key Lab of Computational Biology Shanghai Institute of Nutrition and Health Shanghai China Department of Biomedical Sciences University of Copenhagen Copenhagen Denmark Faculty of Health Sciences and Medicine Bond University Gold Coast QLD Australia Genomics Research Centre Centre for Genomics and Personalised Health School of Biomedical Sciences Queensland University of Technology Brisbane QLD Australia ARC Training Centre for Cell and Tissue Engineering Technologies Queensland University of Technology

We investigated the molecular mechanisms of exercise adaptations in human muscle by integrating genome, methylome, transcriptome, and proteome data from over 1,000 participants (2,340 muscle samples). We identified distinctive signatures associated with maximal oxygen consumption (VO_2max), and multi-omics integration uncovered five key genes as robust exercise markers across layers, with transcription factors functioning as activators, synergizing with DNA methylation to regulate gene expression. Minimal sex differences were observed, while modality-specific analysis highlighted distinct pathways for aerobic and resistance exercise, contrasting with muscle disuse patterns. Finally, we created a webtool, OMAx, featuring our individual omics and integration analysis. These findings provide a comprehensive multi-omics framework for understanding exercise-induced molecular adaptations, offering insights into muscle health, cardiorespiratory fitness, and their roles in aging and disease prevention. © 2025 The Author(s)

关键词： CP: Metabolism DNA methylation epigenetics exercise gene expression Multi Omics proteomic skeletal muscle transcriptomic VO2 max

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Optimization of DC magnetron sputtering deposition process and surface properties of HA-TiO 2 film

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Materials Today: Proceedings 2017年第5期4卷 6372-6380页

作者： P. Premphet M. Prasoetsri D. Boonyawan R. Supruangnet S. Udomsom K. Leksakul Department of Industrial Engineering Faculty of Engineering Chiang Mai University Chiang Mai 50200Thailand Plasma and Beam Physics Research Facility Department of Physics and Materials Science Faculty of Science Chiang Mai University Chiang Mai 50200 Thailand Synchrotron Light Research Institute (Public Organization) 111 University Avenue Nakhon Ratchasima 30000 Thailand Biomedical Engineering Program Faculty of Engineering Chiang Mai University Chiang Mai 50200 Thailand Biomedical Engineering Center Chiang Mai University Chiang Mai 50200 Thailand

Hydroxyapatite (HA) and Titanium (Ti) thin film was fabricated by a pulsed DC magnetron sputtering technique. To synthesize a thin film that compatible with bone cell, optimize parameter of the sputtering process and study of the film properties were carried out. The response surface method (RSM) has been applied in this work and there are 3 considering factors: pressure, power level and a distance between target and substrate. The experiment was conducted by a physical property testing to determine young’s modulus and layer components. The result showed that the differentiation of components and film thickness were related to a variation of a surface coating time. A structure of the film was performed by Atomic Force Microscope (AFM) and X-ray Photoelectron Spectroscopy (XPS). The analysis showed that HA-TiO 2 film was synthesized in sputtering process and the young’s modulus of the film was higher value compared to the bone. Finally, the result of biological property testing proved that HA-TiO 2 film had a bio-compatible property and UV light affect the TiO 2 composition to be a toxic with bone cell.

关键词： Design of experiment HA-Ti thin film Magnetron sputtering X-ray photoelectron spectroscopy UV light

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