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arXiv 2024年

作者： Ohta, Kazuhiro Ono, Satoshi Information Science and Biomedical Engineering Program Kagoshima University Korimoto 1-21-40 Kagoshima890-0065 Japan

Neural Radiance Field (NeRF), capable of synthesizing high-quality novel viewpoint images, suffers from issues like artifact occurrence due to its fixed sampling points during rendering. This study proposes a method t... 详细信息

关键词： Deep neural networks

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Design and development of a modular perfusion bioreactor prototype using 3D printing 46

Design and development of a modular perfusion bioreactor pro...

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46th Annual International Conference of the IEEE engineering in Medicine and Biology Society, EMBC 2024

作者： Cabrera, Rodrigo A. Gonzales Núñez, María A. Rejas Salas, Rosa M. Silva MacEdo, Estefany A. Martinez-Flores, Joaquin E. Torres-Ayala, Lizardo K. Vela, Paulo Castillo, Denis Pontificia Universidad Catolica Del Peru Biomedical Engineering Program PUCP-UPCH Lima Peru Universidad Peruana Cayetano Heredia Biomedical Engineering Program PUCP-UPCH Lima Peru Universidad Peruana Cayetano Heredia Laboratorio de Ingeniería Biomédica Laboratorios de Investigación y Desarrollo Facultad de Ciencias e Ingeniería Av Honorio Delgado 430 SMP Lima15102 Peru Laboratorios de Investigación y Desarrollo Facultad de Ciencias e Ingeniería Honorio Delgado 430 SMP Lima15102 Peru

ISBN: (纸本)9798350371499

This study outlines the conceptual and design framework for a modular bioreactor intended to advance accessibility in tissue engineering by providing an optimal environment for three-dimensional cell growth and differentiation. By integrating 3D printing technology, we propose an innovative and cost-effective approach to bioreactor fabrication streamline the development and customization of tissue engineering applications. While the actual cell cultivation within the bioreactor has yet to be undertaken, preliminary analyses - including rigorous rheological evaluation and computational simulations - validate the bioreactor's potential to maintain a uniform, contamination-free culture environment. This research sets the stage for future experimental work, aiming to validate the bioreactor's effectiveness in supporting cell development and specialization. The anticipated outcomes promise to mark a significant step toward the clinical translation of bone tissue engineering solutions, embodying a new horizon in regenerative medicine. © 2024 IEEE.

关键词： Cell engineering

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A dual-fluorescence assay for gene delivery vehicle screening in macrophages with an inflammation-inducible reporter construct

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BMC methods 2025年第1期2卷 8页

作者： Allie Ivy Shelby N Bess Shilpi Agrawal Varun Kochar Abbey L Stokes Timothy J Muldoon Christopher E Nelson Department of Biomedical Engineering University of Arkansas 120 John A. White Jr. Engineering Hall Fayetteville AR 72701 USA. Cell and Molecular Biology Program University of Arkansas Fayetteville AR USA.

Background:Macrophages are a promising target for therapeutics in various applications such as regenerative medicine and immunotherapy for cancer. Due to their plastic nature, macrophages can switch from a non-activated state to activated with the smallest environmental change. For macrophages to be effective in their respective applications, screening for phenotypic changes is necessary to elucidate the cell response to different delivery vehicles, vaccines, small molecules, and other stimuli. Methods:We created a sensitive and dynamic high-throughput screening method for macrophages based on the activation of NF-κB. For this reporter, we placed an mRFP1 fluorescence gene under the control of an inflammatory promoter, which recruits NF-κB response elements to promote expression during the inflammatory response in macrophages. We characterized the inflammatory reporter based on key markers of an inflammatory response in macrophages including TNF-α cytokine release and immunostaining for inflammatory and non-inflammatory cell surface markers. We compared gene delivery and inflammation of several clinically relevant viral vehicles and commercially available non-viral vehicles. Statistical analysis between groups was performed with a one-way ANOVA with post-hoc Tukey's test. Results:The reporter macrophages demonstrated a dynamic range after LPS stimulation with an EC50 of 0.61 ng/mL that was highly predictive of TNF-α release. Flow cytometry revealed heterogeneity between groups but confirmed population level shifts in pro-inflammatory markers. Finally, we demonstrated utility of the reporter by showing divergent effects with various leading gene delivery vehicles. Discussion:This screening technique developed here provides a dynamic, high-throughput screening technique for determining inflammatory response by mouse macrophages to specific stimuli. The method presented here provides insight into the inflammatory response in mouse macrophages to different viral and non

关键词： Gene delivery Inflammation Macrophage NF-κB Reporter

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Comparison of changes in Ca2+ concentration in quail and rat cardiomyocytes using Fura-4F

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Journal of Biorheology 2023年第2期37卷 138-144页

作者： Ogura, Yuhei Sugita, Shukei Nakamura, Masanori Ujihara, Yoshihiro Department of Electrical and Mechanical Engineering Graduate School of Engineering Nagoya Institute of Technology Gokiso-cho Showa-ku Aichi Nagoya466-8555 Japan Center of Biomedical Physics and Information Technology Nagoya Institute of Technology Gokiso-cho Showa-ku Aichi Nagoya466-8555 Japan Department of Nanopharmaceutical Sciences Nagoya Institute of Technology Gokiso-cho Showa-ku Aichi Nagoya466-8555 Japan

High cardiac pump function in mammals and birds requires the elaborate control of calcium (Ca2+) concentrations in cardiomyocytes (CMs). Previously, we reported that avian quail CMs have a higher capacity for Ca2+ efflux than mammalian rats, but the decrease in Ca2+ concentration estimated using the fluorescent Ca2+ indicator Fura-2 was slower than that expected from the observation of cell relaxation behavior. In this study, we examined changes in Ca2+ concentration in quail and rat CMs using Fura-4F, which allows for more rapid tracking of changes in Ca2+ concentrations than Fura-2. The results demonstrated that, compared to Fura-2, Fura-4F showed a narrower dynamic range but a more rapid response to changes in Ca2+ concentration. Furthermore, Fura-4F showed that the Ca2+ concentration in quail CMs decreased significantly faster than that in rat CMs after electrical stimulation. These results are qualitatively consistent with our previous study using Fura-2 and therefore reinforce the conclusion that the Ca2+ removal capacity of quail CMs is higher than that of rat CMs. © Japanese Society of Biorheology 2023.

关键词： Birds

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Nuclear deformability of cancer cells with different metastatic potential

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Journal of Biorheology 2023年第2期37卷 56-63页

作者： Ujihara, Yoshihiro Ono, Daichi Ito, Megumi Sugita, Shukei Nakamura, Masanori Department of Electrical and Mechanical Engineering Graduate School of Engineering Nagoya Institute of Technology Gokiso-cho Showa-ku Aichi Nagoya466-8555 Japan Department of Nanopharmaceutical Sciences Nagoya Institute of Technology Gokiso-cho Showa-ku Aichi Nagoya466-8555 Japan Center of Biomedical Physics and Information Technology Nagoya Institute of Technology Gokiso-cho Showa-ku Aichi Nagoya466-8555 Japan

During metastasis cancer cells pass through tiny gaps in blood vessel walls and between cells. Since the nucleus is the largest organelle in the cell and is much stiffer than the perinuclear cytoplasm, it may be a major physical constraint for metastasis. Here, we tested the hypothesis that cancer cells with higher metastatic potential have softer nuclei than those with lower metastatic poten-tial. We performed a compression test on cell nuclei isolated from two metastatic B16 melanoma variants (B16-F1 and B16-F10) with different metastatic potentials. The results demonstrated that the nuclei isolated from B16-F1 cells, a lower metastatic cancer cell line, had a significantly higher Young’s modulus than B16-F10 cells, a higher metastatic cancer cell line. We also performed a compression test on the whole cell, encompassing the cell nucleus. Among similar whole-cell strains, B16-F10 cells showed a larger com-pressive strain in the intracellular nucleus than B16-F1 cells. In contrast, the force exerted on the intracellular nuclei of B16-F1 cells was similar to that of B16-F10 cells in the same whole-cell strain. These results suggest that B16-F10 cells may have adopted a strategy of enhancing the deformability of the cell nucleus to improve their meta-static potential. © Japanese Society of Biorheology 2023.

关键词： Cancer cells

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Evaluation of cytokeratin in lymph node metastasis using magnetic nanoparticles and protein aggregates

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IEEE Transactions on Magnetics 2025年

作者： Yabukami, Shin Murayama, Toru Kaneko, Koki Ban, Amane That, Loi Ton Ozawa, Yohei Okamoto, Hiroshi Kamei, Takashi Tanaka, Miyuki Tanaka, Yoshikazu Abe, Takaaki Tohoku University Graduate School of Biomedical Engineering Sendai980-8579 Japan Tohoku University Graduate School of Engineering Sendai980-8579 Japan Tohoku University Graduate School of Medicine Sendai980-8575 Japan Tohoku University Graduate School of Life Sciences Sendai980-8575 Japan

We aim to apply protein sensors to rapid intraoperative diagnosis of lymph node metastasis. In the present study, cytokeratin, which is a biomarker of lymph node metastasis, was bound to protein G-coated magnetic nanoparticles (FG beads, 180 nm) via primary antibody (Anti-Cytokeratin AE1/AE3 Antibody (Monoclonal Mouse IgG1)). The increase of the protein aggregated the magnetic nanoparticles and enhanced the magnetic signal. We have observed magnetic nanoparticles and protein aggregates by cryo-electron microscopy to obtain evidence for this hypothesis. By adding gold nanoparticles to the antibody, we observed the cross-bridging of magnetic nanoparticles via the antigen. Lymph nodes were obtained from two esophageal cancer patients and one non-cancer patient (corrosive esophagitis). Lymph nodes from cancer patients contain cytokeratin, which promotes the aggregation of magnetic nanoparticles and increases the magnetic signal. Statistically significant differences could be assessed between two cancer and non-cancer patients, suggesting the potential of intraoperative rapid diagnosis. © 2025 IEEE. All rights reserved.

关键词： Magnetic nanoparticles

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From Ancient Remedies to Modern Contraptions: Tracing the Evolution of Biocompatible Materials

From Ancient Remedies to Modern Contraptions: Tracing the Ev...

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International Workshop on engineering Methodologies for Medicine and Sports, EMMS 2024

作者： Marin, Elia Basoli, Valentina Ceramic Physics Laboratory Kyoto Institute of Technology Sakyo-ku Matsugasaki Kyoto606-8585 Japan Department of Dental Medicine Graduate School of Medical Science Kyoto Prefectural University of Medicine Kamigyo-ku Kyoto602-8566 Japan Department Polytechnic of Engineering and Architecture University of Udine Udine33100 Italy Biomedical Research Center Kyoto Institute of Technology Sakyo-ku Matsugasaki Kyoto606-8585 Japan Department of Biomedical Engineering Basel University Allschwil4123 Switzerland

ISBN: (纸本)9783031637544

This conference proceeding focuses on the evolution of the concept of biocompatibility, exploring its journey from ancient times to the present day. Evidence points to the use of biomaterials as far back as 5,000 years ago, showcasing early attempts to address medical challenges. Notably gold, which is biologically inert, emerged as a key biomaterial, utilized since the first millennium BC for fixing skull bones and teeth. But it would not take much longer for humanity to start making use of what we now consider bioactive materials, such as calcium carbonate from seashells as well as dentin and enamel harvested from animal sources or even other humans. Surprisingly, the formal definition of biomaterials remained rather vague until the latter half of the 20th century, with the first, comprehensive and widely accepted definition emerging only in 1991. This definition, which comprised both inert and bioactive substances, also emphasized the importance of improving the patients’ quality of life, something that was generally neglected until at least the end 19th century. This retrospective analysis investigates the historical and biological foundations of biocompatibility, tracing its progression from ancient applications rooted in empirical knowledge to the advanced medical implants of the modern era. By understanding this evolution, we gain insights into the foundations and challenges that have shaped and still shape the field of biomaterials science: "what makes a material biocompatible", "how well will it perform over time" and "how much can still be done to improve its biocompatibility" being just a few examples. But the most difficult challenge, from a human and scientific point of view would probably be to accept that "there is no such a thing as a biocompatible material". © The Author(s), under exclusive license to Springer Nature Switzerland AG 2024.

关键词： Biocompatibility

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Detection of physiological changes in rheumatoid arthritis using time-domain near-infrared imaging: an in silico phantom study 15

Detection of physiological changes in rheumatoid arthritis u...

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Optical Tomography and Spectroscopy of Tissue XV 2023

作者： Ioussoufovitch, Seva Diop, Mamadou School of Biomedical Engineering Collaborative Training Program in Musculoskeletal Health Research Bone & Joint Institute Western University London Canada Dept. of Medical Biophysics Western University London Canada Imaging Program Lawson Health Research Institute London Canada

ISBN: (纸本)9781510658578

Early treatment improves rheumatoid arthritis (RA) prognosis;however, 30% of patients still fail their first treatment and current methods can take as long as 3–6 months to detect treatment failure. Time-domain near-infrared diffuse optical imaging (TD-DOI) has the potential to track gradual changes in RA disease activity and could be a more sensitive technique for RA treatment monitoring. Nevertheless, there has been very little investigation into the relationships between TD-DOI and the specific physiological changes that occur in RA. To this end, this work’s objective was to investigate the effects of RA-associated physiological changes on TD-DOI images in silico. Virtual finger phantoms—derived from an MRI segmentation of a healthy human finger—were used to simulate changes in 5 physiological parameters that are typically affected in RA. Four levels of disease activity were considered for each parameter and each parameter was altered individually;parameter alterations were then translated into changes in phantom geometry and optical properties. MCXLAB was used to simulate the propagation of time-domain light (800 nm) through phantoms’ proximal interphalangeal joints and Poisson noise was added to the TD-DOI data to simulate experimental conditions. Spatiotemporal Fourier decomposition was applied to the TD-DOI data to extract image components, which were grouped into datasets based on the physiological parameter that was altered. Component sensitivity to each physiological parameter was assessed using the ratio between a component’s range and standard deviation within each dataset (range-to-error ratio;RER). Mean RER was highest for changes in synovial membrane and fluid volume, suggesting that this parameter may be a primary source of contrast for monitoring RA treatment response with TD-DOI. © 2023 SPIE.

关键词： Phantoms

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A TROPHOBLAST STEM CELL-BASED MODEL OF THE HUMAN PLACENTAL BARRIER 25

A TROPHOBLAST STEM CELL-BASED MODEL OF THE HUMAN PLACENTAL B...

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25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021

作者： Hori, Takeshi Okae, Hiroaki Kobayashi, Norio Arima, Takahiro Kaji, Hirokazu Graduate School of Engineering Tohoku University Japan Graduate School of Medicine Tohoku University Japan Graduate School of Biomedical Engineering Tohoku University Japan

ISBN: (纸本)9781733419031

An organ-on-a-chip is a promising microfluidic device to assess the effect of chemicals on the human body. In this study, we developed a placental barrier-on-a-chip using a microfluidic device and trophoblast stem (TS) cells derived from the human placenta. Under perfusion cultures, TS cells differentiated and formed a trophoblastic barrier having the bilayer structure specific to the placental villi. This placental barrier model would be a useful tool for predicting the health effects of chemicals on the human fetus. © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.

关键词： Stem cells

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BaTiO_(3)@Au nanoheterostructure suppresses triple-negative breast cancer by persistently disrupting mitochondrial energy metabolism

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Nano Research 2023年第2期16卷 2775-2785页

作者： Yanlin Feng Jianlin Wang Xin Ning Aiyun Li Qing You Wanzhen Su Deping Wang Jianyun Shi Lan Zhou Fangfang Cao Xiaoyuan Chen Jimin Cao Key Laboratory of Cellular Physiology at Shanxi Medical University Ministry of Educationand the Department of PhysiologyShanxi Medical UniversityTaiyuan 030001China Departments of Diagnostic Radiology SurgeryChemical and Biomolecular Engineeringand Biomedical EngineeringYong Loo Lin School of Medicine and Faculty of EngineeringNational University of SingaporeSingapore 119074Singapore Clinical Imaging Research Centre Centre for Translational MedicineYong Loo Lin School of MedicineNational University of SingaporeSingapore 117599Singapore Nanomedicine Translational Research Program NUS Center for NanomedicineYong Loo Lin School of MedicineNational University of SingaporeSingapore 117597Singapore

Abnormal metabolism has become a potential target for highly malignant and invasive triple-negative breast cancer(TNBC)due to its relatively low response to traditional *** existing metabolic interventions demonstrated unsatisfactory therapeutic outcomes and potential systemic toxicity,resulting from the metabolic instability and limited targeting ability of inhibitors as well as complex tumor *** address these limitations,here we developed a robust pyroelectric BaTiO_(3)@Au core–shell nanostructure(BTO@Au)to selectively and persistently block energy generation of tumor *** by near-infrared(NIR)laser,the Au shell could generate heat to activate the BaTiO_(3)core to produce reactive oxygen species(ROS)regardless of the constrained microenvironment,thus prominently inhibits mitochondrial oxidative phosphorylation(OXPHOS)and reduces ATP production to induce TNBC cell *** therapeutic effects have been well demonstrated in vitro and in vivo,paving a new way for the development of metabolic interventions.

关键词： pyroelectric nanoheterostructure reactive oxidative species(ROS) metabolic interventions mitochondrial oxidative phosphorylation(OXPHOS) triple-negative breast cancer(TNBC)

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