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Electro osmotically flow of fourth-grade fluid in complex channel with porous medium and lubricated walls: applications in petroleum industry

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Waves in Random and Complex Media 2023年

作者： Nazeer, Mubbashar Pasha, Amjad Ali Hussain, Farooq Adil, Mohammad Mechanical Engineering Program Physical Science and Engineering Division King Abdullah University of Science and Technology Thuwal Saudi Arabia BUITEMS Quetta Pakistan KAUST Clean Combustion Research Center King Abdullah University of Science and Technology Thuwal Saudi Arabia Department of Mathematics Institute of Arts and Sciences Government College University Faisalabad Chiniot Campus Pakistan Aerospace Engineering Department King Abdulaziz University Jeddah Saudi Arabia

This article offers a distinct survey of a multiphase flow through a uniform channel. The electro-osmotic phenomenon is the main source of the flow. Fourth-grade fluid model is used as the base liquid which is suspended with metallic particles to form a bi-phase viscous suspension. Skin friction effects are tackled by employing lubrication effects on the opposite walls of the channel. Porous media is governed by the modified Darcy’s law and the Poisson differential equation is considered for electric double-layer effects. An approximate solution is achieved for the nonlinear fluid flow. The obtained findings are also compared with the existing literature for the limiting case. It is inferred that the shear thickness effects cause the momentum of the bi-phase flow to decline, gradually. However, the motion of the metallic particles is supported by the lubrication effects on the walls of the channel. © 2023 Informa UK Limited, trading as Taylor & Francis Group.

关键词： Porous materials

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Real-Time Probabilistic Reachability Forecasting for Gliders in the Gulf of Mexico

Real-Time Probabilistic Reachability Forecasting for Gliders...

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OCEANS 2024 - Halifax, OCEANS 2024

作者： Mule, E.M. Haley, P.J. Mirabito, C. Dimarco, S.F. Mahmud, S. Dancer, A. Ge, X. Knap, A.H. Liu, Y. Nwankwo, U.C. Glenn, S. Miles, T.N. Aragon, D. Coleman, K. Smith, M. Leber, M. Ramos, R. Storie, J. Stuart, G. Marble, J. Barros, P. Chassignet, E.P. Bower, A. Furey, H.H. De La Cruz, B. Jaimes Shay, L.K. Tenreiro, M. Sanz, E. Pallas Sheinbaum, J. Perez-Brunius, P. Wilson, D. Van Smirren, J. Monreal-Jiménez, R. Salas-De-León, D.A. Tereza, V.K. Contreras Feldman, M. Khadka, M. Lermusiaux, P.F.J. Massachusetts Institute of Technology Department of Mechanical Engineering CambridgeMA United States Texas A&m University Department of Oceanography United States Rutgers University Department of Marine and Coastal Sciences United States Woods Hole Group Inc. United States Fugro Netherlands Department of Earth Ocean and Atmospheric Science Florida State University United States Physical Oceanography Woods Hole Oceanographic Institution United States Rosenstiel School of Marine Atmospheric and Earth Science University of Miami United States Cicese Department of Oceanography Mexico University of the Virgin Islands United States Virgin Islands Ocean Sierra United States Universidad Nacional Autónoma de Mexico Mexico Mexican Institute of Water Technology Mexico Gulf Research Program Nasem United States

ISBN: (纸本)9798331540081

As part of the Mini-Adaptive Sampling Test Run (MASTR) experiment in the Gulf of Mexico (GoM) region from February to April 2024, we demonstrated real-time deterministic and probabilistic reachability analysis and time-optimal path planning to guide a fleet of four ocean gliders. The governing differential equations for reachability analysis and time-optimal path planning were numerically integrated in real-time and forced by currents from our large-ensemble ocean forecasts. We illustrate the real-time deterministic and probabilistic forward reachability analyses, reachability and path planning for glider pickups, time-optimal path planning for gliders in distress, and planning of future glider deployments. Results show that the actual paths of gliders were contained within our reachable set forecasts and in accord with the dynamic reachability fronts. Our time-optimal headings and paths also predicted real glider motions, even for longer-range predictions of weeks to a month duration. Reachability and time-optimal path planning forecasts were successfully employed for glider recovery. They also enabled exploring options for future glider deployments. © 2024 IEEE.

关键词： Gliders

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Real-time Ocean Probabilistic Forecasts, Reachability Analysis, and Adaptive Sampling in the Gulf of Mexico

Real-time Ocean Probabilistic Forecasts, Reachability Analys...

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OCEANS 2024 - Halifax, OCEANS 2024

作者： Lermusiaux, P.F.J. Haley, P.J. Mirabito, C. Mule, E.M. Dimarco, S.F. Dancer, A. Ge, X. Knap, A.H. Liu, Y. Mahmud, S. Nwankwo, U.C. Glenn, S. Miles, T.N. Aragon, D. Coleman, K. Smith, M. Leber, M. Ramos, R. Storie, J. Stuart, G. Marble, J. Barros, P. Chassignet, E.P. Bower, A. Furey, H.H. De La Cruz, B. Jaimes Shay, L.K. Tenreiro, M. Sanz, E. Pallas Sheinbaum, J. Perez-Brunius, P. Wilson, D. Van Smirren, J. Monreal-Jiménez, R. Salas-De-Leon, D.A. Tereza, V.K. Contreras Feldman, M. Khadka, M. Massachusetts Institute of Technology Department of Mechanical Engineering CambridgeMA United States Texas A&m University Department of Oceanography United States Rutgers University Department of Marine and Coastal Sciences United States Woods Hole Group Inc. United States Fugro Netherlands Florida State University Department of Earth Ocean and Atmospheric Science United States Woods Hole Oceanographic Institution Physical Oceanography United States Rosenstiel School of Marine Atmospheric and Earth Science University of Miami United States Cicese Department of Oceanography Mexico University of the Virgin Islands United States Virgin Islands Ocean Sierra United States Universidad Nacional Autónoma de México Mexico Mexican Institute of Water Technology Mexico Nasem Gulf Research Program United States

ISBN: (纸本)9798331540081

The first steps towards integrating autonomous monitoring, probabilistic forecasting, reachability analysis, and adaptive sampling for the Gulf of Mexico were demonstrated in real-time during the collaborative Mini-Adaptive Sampling Test Run (MASTR) ocean experiment, which took place from February to April 2024. The emphasis of this contribution is on the use of the MIT Multidisciplinary Simulation, Estimation, and Assimilation Systems (MSEAS) including Error Subspace Statistical Estimation (ESSE) large-ensemble forecasting and path planning systems to predict ocean fields and uncertainties, forecast reachable sets and optimal paths for gliders, and guide sampling aircraft and ocean vehicles toward the most informative observations. Deterministic and probabilistic ocean forecasts are exemplified and linked to the variability of the Loop Current (LC) and LC Eddies, demonstrating predictive skill by real-time comparisons to independent data. Risk forecasts in terms of probabilities of currents exceeding 1.5 kt were provided. The most informative sampling patterns for Remote Ocean Current Imaging System (ROCIS) flights were forecast using mutual information between surface currents and density anomaly. Finally, we guided four underwater gliders using probabilistic reachability and path-planning forecasts. © 2024 IEEE.

关键词： Gliders

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Diet as an epigenetic factor in inflammatory bowel disease

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World Journal of Gastroenterology 2023年第41期29卷 5618-5629页

作者： Karina Marangoni Gilson Dorneles Daniella Miranda da Silva Letícia Pereira Pinto Carina Rossoni Sabrina Alves Fernandes Egas Moniz School of Health and Science Caparica-AlmadaPortugalCaparica 2820-062Portugal National Institute of Sciences and Technology-Theranostics and Nanobiotechnology Federal University of Uberlandia-MGBrazilUberlândia 38400-902Brazil Corporate Social Responsibility Hospital Moinhos de VentoPorto Alegre 90035-004Brazil Postgraduate Program in Gastroenterology Universidade Federal do Rio Grande do SulPorto Alegre 91540-000Brazil Department of Nutrition Uniasselvi-Group VitruSanta Catarina 89082-262Brazil Postgraduate Program in Hepatology Universidade Federal de Ciências da Saúde de Porto AlegrePorto Alegre 90050-170Brazil Master in Physical Activity and Health Polytechnic Institute of BejaBeja 7800-000Portugal Degree in Nutrition Sciences Lusófona UniversityLisboa 1749-024Portugal

Inflammatory bowel disease(IBD)has as a main characteristic the exacerbation of the immune system against enterocytes,compromising the individual’s intestinal *** inflammatory cascade causes several nutritional deficiencies,which further compromise immunological functioning and,as a result,worsen the *** vicious cycle can be interrupted as the patient’s dietary pattern meets their needs according to their clinical condition,acting directly on the inflammatory process of IBD through the interaction of food,intestinal microbiota,and *** nutritional intervention for IBD has a crucial role in preventing and managing disease *** review addresses epigenetic modifications through dietary compounds as a mechanism for modulating the intestinal microbiota of patients with IBD.

关键词： Inflammatory bowel disease Epigenetics Nutrition Nutrigenetics

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The growing memristor industry

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Nature 2025年第8059期640卷 613-622页

作者： Lanza, Mario Pazos, Sebastian Aguirre, Fernando Sebastian, Abu Le Gallo, Manuel Alam, Syed M. Ikegawa, Sumio Yang, J. Joshua Vianello, Elisa Chang, Meng-Fan Molas, Gabriel Naveh, Ishai Ielmini, Daniele Liu, Ming Roldan, Juan B. Department of Materials Science and Engineering National University of Singapore Singapore Singapore Institute for Functional Intelligent Materials National University of Singapore Singapore Singapore Centre for Advanced 2D Materials (CA2DM) National University of Singapore Singapore Singapore Materials Science and Engineering Program Physical Science and Engineering Division King Abdullah University of Science and Technology (KAUST) Thuwal Saudi Arabia Intrinsic Semiconductor Technologies London United Kingdom IBM Research – Zurich Rüschlikon Switzerland Everspin Technologies Chandler AZ United States Department of Electrical and Computer Engineering University of Southern California Los Angeles CA United States CEA-Leti Université Grenoble Alpes Grenoble France Department of Electrical Engineering National Tsing Hua University Hsinchu Taiwan Weebit Nano Hod Hasharon Israel Dipartimento di Elettronica Informazione e Bioingegneria (DEIB) Politecnico di Milano and *** Milano Italy Key Laboratory of Microelectronic Devices and Integrated Technology Institute of Microelectronics Chinese Academy of Sciences Beijing China Departamento de Electrónica y Tecnología de Computadores Universidad de Granada Facultad de Ciencias Granada Spain

The semiconductor industry is experiencing an accelerated transformation to overcome the scaling limits of the transistor and to adapt to new requirements in terms of data storage and computation, especially driven by artificial intelligence applications and the Internet of Things. In this process, new materials, devices, integration strategies and system architectures are being developed and optimized. Among them, memristive devices and circuits—memristors are two-terminal memory devices that can also mimic some basic bioelectronic functions—offer a potential approach to create more compact, energy-efficient or better-performing systems. The memristor industry is growing quickly, raising abundant capital investment, creating new jobs and placing advanced products in the market. Here we analyse the status and prospects of the memristor industry, focusing on memristor-based products that are already commercially available, prototypes with a high technological readiness level that might affect the market in the near future, and discuss obstacles and pathways to their implementation. © Springer Nature Limited 2025.

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Rhodiola and Salidroside Attenuate Oxidative Stress-Triggered H9c2 Cardiomyoblast Apoptosis Through IGF1R-Induced ERK1/2 Activation

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Environmental Toxicology 2024年第11期39卷 5150-5161页

作者： Ju, I-Ju Tsai, Bruce Chi-Kang Kuo, Wei-Wen Kuo, Chia-Hua Lin, Yueh-Min Hsieh, Dennis Jine-Yuan Pai, Pei-Ying Huang, Shang-En Lu, Shang-Yeh Lee, Shin-Da Huang, Chih-Yang Division of General Medicine Department of Medical Education Tri-Service General Hospital National Defense Medical Center Taipei Taiwan Department of Neurological Surgery Tri-Service General Hospital National Defense Medical Center Taipei Taiwan Cardiovascular and Mitochondrial Related Disease Research Center Hualien Tzu Chi Hospital Buddhist Tzu Chi Medical Foundation Hualien Taiwan Department of Biological Science and Technology College of Life Sciences China Medical University Taichung Taiwan Ph.D. Program for Biotechnology Industry China Medical University Taichung Taiwan School of Pharmacy China Medical University Taichung Taiwan Laboratory of Exercise Biochemistry University of Taipei Taipei Taiwan Institute of Sports Sciences University of Taipei Taipei Taiwan Department of Kinesiology and Health Science College of William and Mary WilliamsburgVA United States School of Physical Education and Sports Science Soochow University Jiangsu Suzhou China Department of Pathology Changhua Christian Hospital Changhua Taiwan School of Medicine Chung Shan Medical University Taichung Taiwan School of Medical Laboratory and Biotechnology Chung Shan Medical University Taichung Taiwan Clinical Laboratory Chung Shan Medical University Hospital Taichung Taiwan Division of Cardiovascular Medicine Department of Internal Medicine China Medical University Hospital Taichung Taiwan School of Medicine College of Medicine China Medical University Taichung Taiwan Graduate Institute of Chinese Medicine China Medical University Taichung Taiwan Graduate Institute of Biomedical Sciences China Medical University Taichung Taiwan School of Rehabilitation Science Shanghai University of Traditional Chinese Medicine Shanghai China School of Rehabilitation Medicine Weifang Medical University Shandong Weifang China Department of Physical Therapy Graduate Institute of Rehabilitation Science China Medical University Taichung Taiwan Department of Medical Research China Medic

Oxidative stress is a pivotal factor in the pathogenesis of various cardiovascular diseases. Rhodiola, a traditional Chinese medicine, is recognized for its potent antioxidant properties. Salidroside, a phenylpropanoid glycoside derived from Rhodiola rosea, has shown remarkable antioxidant capabilities. This study aimed to elucidate the potential protective mechanisms of Rhodiola and salidroside against H2O2-induced cardiac apoptosis in H9c2 cardiomyoblast cells. H9c2 cells were exposed to H2O2 for 4 h, and subsequently treated with Rhodiola or salidroside for 24 h. Cell viability and apoptotic pathways were assessed. The involvement of insulin-like growth factor 1 receptor (IGF1R) and the activation of extracellular regulated protein kinases 1/2 (ERK1/2) were investigated. H2O2 (100 μM) exposure significantly induced cardiac apoptosis in H9c2 cells. However, treatment with Rhodiola (12.5, 25, and 50 μg/mL) and salidroside (0.1, 1, and 10 nM) effectively attenuated H2O2-induced cytotoxicity and apoptosis. This protective effect was associated with IGF1R-activated phosphorylation of ERK1/2, leading to the inhibition of Fas-dependent proteins, HIF-1α, Bax, and Bak expression in H9c2 cells. The images from hematoxylin and eosin staining and immunofluorescence assays also revealed the protective effects of Rhodiola and salidroside in H9c2 cells against oxidative damage. Our findings suggest that Rhodiola and salidroside possess antioxidative properties that mitigate H2O2-induced apoptosis in H9c2 cells. The protective mechanisms involve the activation of IGF1R and subsequent phosphorylation of ERK1/2. These results propose Rhodiola and salidroside as potential therapeutic agents for cardiomyocyte cytotoxicity and apoptosis induced by oxidative stress in heart diseases. Future studies may explore their clinical applications in cardiac health. © 2024 Wiley Periodicals LLC.

关键词： Cell death

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Strong, Mid-IR Driven THz Generation for Nonlinear Spectroscopy

Strong, Mid-IR Driven THz Generation for Nonlinear Spectrosc...

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Conference on Lasers and Electro-Optics (CLEO)

作者： Claudia Gollner Rokas Jutas Dominik Kreil Dmitry N. Dirin Simon C. Boehme Andrius Baltuška Maksym V. Kovalenko Anastasios D. Koulouklidis Vladimir Yu. Fedorov Stelios Tzortzakis Mostafa Shalaby Audrius Pugžlys SLAC National Accelerator Laboratory Stanford Institute for Materials & Energy Sciences Menlo Park CA United States Photonics Institute TU Wien Wien Austria Institute for Theoretical Physics Johannes Kepler University Linz Austria Department of Chemistry and Applied Biosciences Institute of Inorganic Chemistry ETH Zürich Zürich Switzerland Empa-Swiss Federal Laboratories for Materials Science and Technology Dübendorf 12 Switzerland Center for Physical Sciences & Technology Vilnius Lithuania Institute of Electronic Structure and Laser (IESL) Foundation for Research and Technology - Hellas (FORTH) Heraklion Greece Science Program Texas A&M University at Qatar Doha Qatar P.N. Lebedev Physical Institute of the Russian Academy of Sciences Moscow Russia Department of Materials Science and Technology University of Crete Heraklion Greece Techpark Swiss Terahertz Research-Zurich Zurich Switzerland Key Laboratory of Terahertz Optoelectronics Beijing Advnaced Innovation Center of Imaging Technology CNU Bejing China

We report on strong-field THz sources from optical rectification in organic crystals or two-color plasma filaments, driven by intense mid-IR pulses. Non-linear perturbation of semiconductor materials is demonstrated b... 详细信息

ISBN: (纸本)9798350369311

关键词： Spectroscopy Semiconductor materials Semiconductor lasers Perturbation methods Lasers and electrooptics Plasmas Transient analysis Nonlinear optics Electro-optical waveguides Optical pulses

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Extensibility governs the flow-induced alignment of polymers and rod-like colloids

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physical Review Research 2024年第1期6卷 L012042-L012042页

作者： Vincenzo Calabrese Tatiana Porto Santos Carlos G. Lopez Minne Paul Lettinga Simon J. Haward Amy Q. Shen Micro/Bio/Nanofluidics Unit Okinawa Institute of Science and Technology Graduate University 1919-1 Tancha Onna-son Okinawa 904-0495 Japan RWTH Aachen University Institute of Physical Chemistry Landoltweg 2 52074 Aachen Germany Laboratory for Soft Matter and Biophysics KU Leuven Celestijnenlaan 200D B-3001 Leuven Belgium ICS-3 Institut für Weiche Materie Forschungszentrum Jülich D-52425 Jülich Germany

Polymers and rod-like colloids (PaRC) adopt a favorable orientation under sufficiently strong flows. However, how the flow kinematics affect the alignment of such nanostructures according to their extensibility remains unclear. By analyzing the shear- and extension-induced alignment of chemically and structurally different PaRC, we show that extensibility is a key determinant of the structural response to the imposed kinematics. We propose a unified description of the effectiveness of extensional flow, compared to shearing flow, at aligning PaRC of different extensibility.

关键词： Colloids Complex fluids Polymers

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technology Roadmap for Flexible Sensors

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ACS NANO 2023年第6期17卷 5211-5295页

作者： Luo, Yifei Abidian, Mohammad Reza Ahn, Jong-Hyun Akinwande, Deji Andrews, Anne M. Antonietti, Markus Bao, Zhenan Berggren, Magnus Berkey, Christopher A. Bettinger, Christopher John Chen, Jun Chen, Peng Cheng, Wenlong Cheng, Xu Choi, Seon-Jin Chortos, Alex Dagdeviren, Canan Dauskardt, Reinhold H. Di, Chong-an Dickey, Michael D. Duan, Xiangfeng Facchetti, Antonio Fan, Zhiyong Fang, Yin Feng, Jianyou Feng, Xue Gao, Huajian Gao, Wei Gong, Xiwen Guo, Chuan Fei Guo, Xiaojun Hartel, Martin C. He, Zihan Ho, John S. Hu, Youfan Huang, Qiyao Huang, Yu Huo, Fengwei Hussain, Muhammad M. Javey, Ali Jeong, Unyong Jiang, Chen Jiang, Xingyu Kang, Jiheong Karnaushenko, Daniil Khademhosseini, Ali Kim, Dae-Hyeong Kim, Il-Doo Kireev, Dmitry Kong, Lingxuan Lee, Chengkuo Lee, Nae-Eung Lee, Pooi See Lee, Tae-Woo Li, Fengyu Li, Jinxing Liang, Cuiyuan Lim, Chwee Teck Lin, Yuanjing Lipomi, Darren J. Liu, Jia Liu, Kai Liu, Nan Liu, Ren Liu, Yuxin Liu, Yuxuan Liu, Zhiyuan Liu, Zhuangjian Loh, Xian Jun Lu, Nanshu Lv, Zhisheng Magdassi, Shlomo Malliaras, George G. Matsuhisa, Naoji Nathan, Arokia Niu, Simiao Pan, Jieming Pang, Changhyun Pei, Qibing Peng, Huisheng Qi, Dianpeng Ren, Huaying Rogers, John A. Rowe, Aaron Schmidt, Oliver G. Sekitani, Tsuyoshi Seo, Dae-Gyo Shen, Guozhen Sheng, Xing Shi, Qiongfeng Someya, Takao Song, Yanlin Stavrinidou, Eleni Su, Meng Sun, Xuemei Takei, Kuniharu Tao, Xiao-Ming Tee, Benjamin C. K. Thean, Aaron Voon-Yew Trung, Tran Quang Wan, Changjin Wang, Huiliang Wang, Joseph Wang, Ming Wang, Sihong Wang, Ting Wang, Zhong Lin Weiss, Paul S. Wen, Hanqi Xu, Sheng Xu, Tailin Yan, Hongping Yan, Xuzhou Yang, Hui Yang, Le Yang, Shuaijian Yin, Lan Yu, Cunjiang Yu, Guihua Yu, Jing Yu, Shu-Hong Yu, Xinge Zamburg, Evgeny Zhang, Haixia Zhang, Xiangyu Zhang, Xiaosheng Zhang, Xueji Zhang, Yihui Zhang, Yu Zhao, Siyuan Zhao, Xuanhe Zheng, Yuanjin Zheng, Yu-Qing Zheng, Zijian Zhou, Tao Zhu, Bowen Zhu, Ming Zhu, Rong Zhu, Yangzhi Zhu, Yong Zou, Guijin Chen, Xiaodong 08-03 Innovis Singapore 138634 Republic of Singapore Innovative Centre for Flexible Devices (iFLEX) School of Materials Science and Engineering Nanyang Technological University Singapore 639798 Singapore Department of Biomedical Engineering University of Houston Houston Texas 77024 United States School of Electrical and Electronic Engineering Yonsei University Seoul 03722 Republic of Korea Department of Electrical and Computer Engineering The University of Texas at Austin Austin Texas 78712 United States Microelectronics Research Center The University of Texas at Austin Austin Texas 78758 United States Department of Chemistry and Biochemistry California NanoSystems Institute and Department of Psychiatry and Biobehavioral Sciences Semel Institute for Neuroscience and Human Behavior and Hatos Center for Neuropharmacology University of California Los Angeles Los Angeles California 90095 United States Colloid Chemistry Department Max Planck Institute of Colloids and Interfaces 14476 Potsdam Germany Department of Chemical Engineering Stanford University Stanford California 94305 United States Laboratory of Organic Electronics Department of Science and Technology Campus Norrköping Linköping University 83 Linköping Sweden Wallenberg Initiative Materials Science for Sustainability (WISE) and Wallenberg Wood Science Center (WWSC) SE-100 44 Stockholm Sweden Department of Materials Science and Engineering Stanford University Stanford California 94301 United States Department of Biomedical Engineering and Department of Materials Science and Engineering Carnegie Mellon University Pittsburgh Pennsylvania 15213 United States Department of Bioengineering University of California Los Angeles Los Angeles California 90095 United States School of Chemistry Chemical Engineering and Biotechnology Nanyang Technological University Singapore 637457 Singapore Nanobionics Group Department of Chemical and Biological Engineering Monash University Clayton Australia 3800 Monash

Humans rely increasingly on sensors to address grand challenges and to improve quality of life in the era of digitalization and big data. For ubiquitous sensing, flexible sensors are developed to overcome the limitations of conventional rigid counterparts. Despite rapid advancement in bench-side research over the last decade, the market adoption of flexible sensors remains limited. To ease and to expedite their deployment, here, we identify bottlenecks hindering the maturation of flexible sensors and propose promising solutions. We first analyze continued...challenges in achieving satisfactory sensing performance for real-world applications and then summarize issues in compatible sensor-biology interfaces, followed by brief discussions on powering and connecting sensor networks. Issues en route to commercialization and for sustainable growth of the sector are also analyzed, highlighting environmental concerns and emphasizing nontechnical issues such as business, regulatory, and ethical considerations. Additionally, we look at future intelligent flexible sensors. In proposing a comprehensive roadmap, we hope to steer research efforts towards common goals and to guide coordinated development strategies from disparate communities. Through such collaborative

关键词： soft materials mechanics engineering flexible electronics conformable sensors bioelectronics human-machine interfaces body area sensor networks technology translation sustainable electronics

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Instant multicolor super-resolution microscopy with deep convolutional neural network

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biophysics Reports 2021年第4期7卷 304-312页

作者： Songyue Wang Chang Qiao Amin Jiang Di Li Dong Li National Laboratory of Biomacromolecules CAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of Sciences College of Life Sciences University of Chinese Academy of Sciences Department of Automation Tsinghua University Hefei National Laboratory for Physical Sciences at Microscale and School of Life Sciences University of Science and Technology of China Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory)

Multicolor super-resolution(SR) microscopy plays a critical role in cell biology research and can visualize the interactions between different organelles and the cytoskeleton within a single cell. However,more color channels bring about a heavier budget for imaging and sample preparation, and the use of fluorescent dyes of higher emission wavelengths leads to a worse spatial resolution. Recently, deep convolutional neural networks(CNNs) have shown a compelling capability in cell segmentation, superresolution reconstruction, image restoration, and many other aspects. Taking advantage of CNN's strong representational ability, we devised a deep CNN-based instant multicolor super-resolution imaging method termed IMC-SR and demonstrated that it could be used to separate different biological components labeled with the same fluorophore, and generate multicolor images from a single superresolution image in silico. By IMC-SR, we achieved fast three-color live-cell super-resolution imaging with ～100 nm resolution over a long temporal duration, revealing the complicated interactions between multiple organelles and the cytoskeleton in a single COS-7 cell.

关键词： Multicolor imaging Super-resolution Convolutional neural network

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