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The effects of aqueous two-phase system on the enrichment of quercetin from Strobilanthes crispus L.

AIP Conference Proceedings 2021年第1期2376卷

作者： Rita Arbianti Linatri Purwati Latifah Fauzul Fadli Amalia Larasati Tania Surya Utami Yuswan Muharam Slamet Bioprocess Engineering Study Program Department of Chemical Engineering University of Indonesia Depok. Indonesia

Enrichment of quercetin from Strobilanthes crispus L. in this study was carried out by ethanol/ammonium sulfate system combined with ultrasonic technology. The optimal partition coefficient (31,179) and recovery (98,043%) of quercetin were obtained in a system composed 33% ethanol (w/w) and 14% (NH4)2SO4 (w/w), 20:1 liquid-to-solid ratio, and extraction temperature of 27°C. Based on concentration and yield, the purity of quercetin increased by 660.88% and 172.89% after involving ATPS, respectively. The use of ATPS obtained 44.717±0,295 mg/L quercetin with a yield of 3.504±0,0231 mg/g.

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Improved performance of stacked microbial desalination cell as oil waste model treatment by adding ion exchange resin and activated carbon

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AIP Conference Proceedings 2021年第1期2376卷

作者： Hasna Aprilia Jelita Ninda Qorina Rita Arbianti Tania Surya Utami Bioprocess Engineering Study Program Department of Chemical Engineering Universitas Indonesia Depok. Indonesia

One way to treat oil waste is a seawater desalination system that uses exoelectorgenic bacteria as an agent for the degradation of organic compounds contained in oil waste. Microbial Desalination Cell (MDC) is a development of Microbial Fuel Cell (MFC), a method that can eliminate salt content in seawater using electricity generated by bacteria from wastewater. Stacked Microbial Desalination Cell (SMDC) is an MDC development where SMDC uses many pairs of ion Exchange Membranes (IEMs) where IEMs are placed between the Anion Exchange Membrane (AEM) and the Cation Exchange Membrane (CEM). This is intended to increase the efficiency of electron transfer. SMDC can also return more energy than other types of MDC so that the cost is more effectively. In this research, using a 2-SMDC reactor configuration with graphite rods as anode, CFC coated with activated carbon as a cathode, potassium permanganate as catholyte, and adding ion exchange resin to salt chamber. Independent variables used in this research were activated carbon mass variations of 0, 2, and 4 g. Parameters that will be obtained are COD, electrical productivity, and pH. The results obtained in this study indicate that the optimum mass variation of activated carbon is 4 g by adding Ion Exchange resin (ratio Resin Na and Cl 1 : 1) with a COD reduction of 57.808% and produces electrical productivity of 0.000561 W/m3 , and the change in pH by 0.24.

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The effect of incubation time variations on the enrichment of linoleic acid in rice bran oil by solid fermentation method using Aspergillus terreus

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AIP Conference Proceedings 2021年第1期2376卷

作者： Agustina Evania Dewi Rita Arbianti Tania Surya Utami Bioprocess Engineering Study Program Department of Chemical Engineering Universitas Indonesia Depok. Indonesia

Rice bran oil contains unsaturated fatty acids which are very potential to prevent and reduce the risk of hypercholesterolemia. Hypercholesterolemia is a condition of increasing concentration of cholesterol in the blood that exceeds the normal value. This condition can be prevented by consuming unsaturated fatty acids, such as linoleic acid (C18:2). Hypocolesterolemic effects possessed by rice bran oil can reduce blood cholesterol levels thereby reducing the risk of atherosclerosis and coronary heart disease. Rice bran is the most effective substrate for producing that type of fatty acids. Its fermentation using Aspergillus terreus mold is one of the ways to maintain and enrich the unsaturated fatty acid content in rice bran oil. Aspergillus terreus can produce oil that has a higher composition of unsaturated fatty acids compared to another Aspergillus species. This study examines the effect of incubation time of mold inoculum to determine the content of unsaturated fatty acids in fermented bran oil. The fermentation method used is solid fermentation using the modified Bligh-Dyer extraction method. Gas Chromatography/Mass Spectrometry (GC/MS) is an instrument which is used to determine the composition and content of unsaturated fatty acids in rice bran oil. The result showed that the incubation time for the enrichment of linoleic acid in rice bran oil using Aspergillus terreus, which is for 6 days with linoleic acid content of originally 37,758% to 39,780%.

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The effect of pH of the medium and inoculum volume variations on the enrichment of unsaturated fatty acid in rice bran oil by solid fermentation method using Aspergillus terreus

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AIP Conference Proceedings 2021年第1期2376卷

作者： Daffa Ramadhan Aridis Luthfi Meizar Pradana Rita Arbianti Tania Surya Utami Bioprocess Engineering Study Program Department of Chemical Engineering Universitas Indonesia Depok. Indonesia

The rice milling process will produce side products, one of them is rice bran which has enormous potential both in terms of economy and health if it is further processed, but until now the use of rice bran is still very limited. Rice bran contains high oil content, which is around 10-23% consisting of 80% unsaturated fatty acids and 20% saturated fatty acids. Unsaturated fatty acids are generally obtained from fish oil, but fish oil has a high price and smells fishy. The content of unsaturated fatty acids in rice bran can be enriched by using Aspergillus terreus, because these molds can produce unsaturated fatty acid higher than other types of Aspergillus. This study examines the effect of pH of the medium variations and the volume of the mold variations on increasing unsaturated fatty acid content in fermented rice bran oil using Aspergillus terreus. The fermentation method used is solid-state fermentation with modified Bligh-Dyer extraction method and Gas Chromatography/Mass Spectrometry (GC-MS) instrument is used to determine the composition and content of unsaturated fatty acids in bran oil. The result showed that the optimum pH of the medium for increasing the unsaturated fatty acid contents in rice bran oil using Aspergillus terreus, which is at pH 5 with the initial unsaturated fatty acid content of 74.835% to 76.719%. For the optimum inoculum volume to increasing the unsaturated fatty acids contents in rice bran oil is 7 mL with the acid content of unsaturated fatty acids (linoleic acid) of 37.9% to 41.394%.

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Initial Analysis of Plastic Debris Accumulation in the Estuary of Wonorejo River, Surabaya, Indonesia 6

Initial Analysis of Plastic Debris Accumulation in the Estua...

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6th Environmental Technology and Management Conference, ETMC 2019

作者： Kurniawan, Setyo Budi Imron, Muhammad Fauzul Department of Chemical and Bioprocess Engineering Faculty of Engineering and Built Environment Universiti Kebangsaan Malaysia Bangi Selangor43600 Malaysia Department of Environmental Engineering Faculty of Civil Environmental and Geo Engineering Institut Teknologi Sepuluh Nopember Kampus ITS Keputih Sukolilo Surabaya60111 Indonesia Study Program of Environmental Engineering Department of Biology Faculty of Science and Technology Universitas Airlangga Kampus C UNAIR Jalan Mulyorejo Surabaya60115 Indonesia

The purpose of this research was to investigate the accumulation of plastic debris in the Wonorejo River Estuary, Surabaya, Indonesia. Visible plastic debris were collected from three (3) sampling points along the intertidal area of Wonorejo River Estuary. The correlation between sampling points (SPs) and the amount of the collected plastic debris (CPD) was analysed using one-way ANOVA. Result of one-way ANOVA showed that the sampling point was significantly affect the amount of the collected plastic debris (p2 from SP1, 375.97±16.72 g dry weight/m2 from SP2, and 291.13±36.28 g dry weight/m2 from SP3. The highest percentage of collected debris item was plastic bags (up to 57.90%), followed by bottle caps (up to 16.65%). The most identified plastic types were Low-Density Polyethylene (LDPE) (up to 73.13%), followed by Polypropylene (PP) (up to 17.22%). Understanding the accumulation of plastic debris in estuary is a fundamental requirement to conduct an advance research related to the marine plastic pollution and to determine further actions to solve this problem. © 2020 The Authors, published by EDP Sciences.

关键词： Analysis of variance (ANOVA)

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The optimization of ultrasonic-assisted extraction of antioxidant compounds from butterfly pea flower (Clitoria ternatea L.) by using response surface methodology

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IOP Conference Series: Earth and Environmental Science 2021年第1期743卷

作者： N Izza D Tristantini Bioprocess Engineering Study Program Faculty of Agricultural Technology Universitas Brawijaya Department of Chemical Engineering Faculty of Engineering Universitas Indonesia

Butterfly pea flower (Clitoria ternatea L) contains many phytochemical compounds that are beneficial to human health, one of which is incorporated in the group of phenolic compounds. Generally, the extraction process of an organic compound requires heat and takes a relatively long time. Therefore, the application of the ultrasonic method for the extraction of butterfly pea flower is studied. This study aims to obtain the best-operating conditions in the ultrasonic-assisted extraction of the phenolic compound of the butterfly pea flower. The optimization of operating conditions during the extraction process was performed by using Response Surface Methodology (RSM) with the application of Design Expert 10 software. The results of the optimum operating conditions are 62,063 ml/g of solvent and flower ratio, 60% ultrasonic power output, and 15 minutes of extraction time. These operating conditions result in 55.834 ml of extract, 0.874 mg/ml of total phenolic content (TPC), and 10.42 mg/l of total anthocyanins (TA).

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Isolation and optimization of polyhydroxybutyrate from Chlorella vulgaris using NaClO and ultrasonication aided chemical pretreatment methods

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AIP Conference Proceedings 2021年第1期2344卷

作者： Ajeng Inas Setyorini Dianursanti Bioprocess Engineering Study Program Department of Chemical Engineering Faculty of Engineering Universitas Indonesia Kampus UI Depok West Java 16424 Indonesia

The increasing use of food packaging makes plastic waste the biggest environmental issue. Since the use of conventional plastic comes from fossil polymers, so it is difficult to be decomposed by bacteria. The right solution is to replace it with bioplastics. This study uses Chlorella vulgaris as a producer of Poly-β-hydroxybutyrate (PHB). PHB has characteristics such as biodegradability, thermoplasticity, hydrophobicity, biocompatibility with cells and tissues, and physical properties similar to polypropylene, which can potentially apply in food, pharmaceutical, and medical packaging. PHB is obtained by isolating biomass from Chlorella vulgaris. This research studied PHB isolation using chemical and mechanical cell disruption methods, namely NaClO and sonication, with variations in solvent concentration. The simplest and most economical method of isolation of C. vulgaris is the general stages of cell disruption, PHB precipitation, and PHB purification. The quantifying PHB method is by calculating the yield of PHB, then identifying PHB by FTIR, and testing the quality of the best PHB compounds by GCMS. The parameters used in this study are yield, PHB physical results, and PHB test results. In the condition of 0.6% w/v sample concentration and 1.37% w/v solvent concentration using ultrasonication, the best PHB isolation yield was 37.2%. GCMS sample results showed the presence of n-hexadecanoic acid (palmitic acid), 9-Octadecanoic acid (oleic acid), 2-Palmitoylglycerol, and Octadecanoic acid (stearic acid) as the main compounds that could potentially be bioplastic materials.

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The enhancement of phycocyanin yield and purity from Spirulina platensis using freeze-thawing method on various solvents

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AIP Conference Proceedings 2021年第1期2344卷

作者： Dianursanti Maulina Aini Hafidzah Bioprocess Engineering Study Program Department of Chemical Engineering Faculty of Engineering Universitas Indonesia. Kampus UI Depok West Java 16424 Indonesia

Phycocyanin is a pigment compound that can be found from Spirulina platensis microalgae. It has been used for food colorant, cosmetic dye and fluorescence reagent for clinical diagnosis. Phycocyanin can be extracted using various procedure that was affected by many factors, one of them is the type of solvent that was used in the extraction process. This research will study the usage of different solvents, using sodium phosphate buffer and calcium chloride. The extraction method used in this research is freeze-thawing. The parameters of this extraction are phycocyanin yield and purity. The obtained result shows a higher yield of phycocyanin from extraction using phosphate buffer, with the value of 35.31 mg/g, and a higher phycocyanin purity from extraction using CaCl2 with the ratio of 2.6.

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Aerobic-pond Palm Oil Mill Effluent (POME) utilization as growth medium of Scenedesmus obliquus for lipid production

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IOP Conference Series: Earth and Environmental Science 2021年第1期737卷

作者： C A Situmeang R Sianturi Y Lestari M M Martgrita Bioprocess Engineering Study Program Faculty of Biotechnology Institut Teknologi Del Laguboti Toba Samosir 22381 North Sumatera Indonesia

The purpose of this research is to know the growth rate and biomass production of Scenedesmus obliquus in palm oil mill effluent (POME) medium taken from aerobic pond and to know the lipid concentration produced by S. obliquus. In this research, S. obliquus was cultured in various POME concentration, those were 20, 60, 80, 90, 95, and 100% v/v, while the concentration of inoculum was 30% v/v, and fatty acid content was analysed by gas chromatography. The results revealed that growth rate was decrease with the increasing concentration of POME (0.3545, 0.1792, 0.1566, 0.1268, 0.1158, and 0.1008/day), dry biomass weight was increase with the increasing concentration of POME (0.40, 1.20, 1.30, 1.77, 2.28, and 2.45 g/L), and lipid content also tended to increase with the increasing concentration of POME (13.10%, 36.59%, 36.65%, 50.37%, 60.40%, and 64.98%). Oleic acid and behenic acid were the dominant fatty acid content in S. obliquus lipid cultured in 100% concentration POME media. It is concluded that aerobic-pond POME can be utilized as growth medium for S. obliquus with a high lipid content.

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Utilization of Spirulina platensis microalgae as edible coating to maintain quality of fresh strawberry (Fragaria sp.)

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AIP Conference Proceedings 2021年第1期2344卷

作者： Prayoga Byantara Dianursanti 1Bioprocess Engineering Study Program Department of Chemical Engineering Faculty of Engineering Universitas Indonesia Kampus UI Depok West Java 16424 Indonesia

The fruit is an important element of daily food but undergoes rapid deterioration due to their high metabolic activity. The edible coating on fruit is one alternative that can be used to improve quality and prolong shelf life. The edible coating can come from microalgae with high protein content, which is Spirulina platensis. Other materials needed are glycerol as a plasticizer to increase flexibility and elasticity and surfactant, which is carboxymethyl cellulose (CMC) as a thickener, stabilizer, and emulsifier. Strawberry (Fragaria sp.) was a sample fruit in this study. The condition of storage temperature is at the fridge temperature of about ± 4-7 °C. This study aims to obtain the appropriate microalgae concentration as the main ingredient in making edible coating to prolong strawberry’s shelf life. In this study, what varied are the concentration of S. platensis microalgae, which are 0,5%, 0,75%, and 1% (w/v). There are three tests carried out: protein quantification on edible coating solution, physical properties (weight loss), and chemical properties (pH and vitamin C) on fruit. Based on this study, an edible coating that has the best effect on strawberries is 1% S. platensis with lower weight loss, pH level, and higher vitamin C content up to seven days of storage. With higher protein content, the use of 1% S. platensis with the temperature of ± 4-7 °C achieved the lowest weight loss at 5.21 ± 0.09%, the lowest pH value at 3.29 ± 0.06, and the highest vitamin C content at 37.51 ± 0.78 mg/100g in strawberries in the seventh day of storage.

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