检索结果-内蒙古大学图书馆

Increased deformations are dispensable for encapsulated cell mechanoresponse in engineered bone analogs mimicking aging bone marrow

引用

Mechanobiology in Medicine 2025年第1期3卷 57-67页

作者： Alexander M.Regner Maximilien DeLeon Kalin D.Gibbons Sean Howard Derek Q.Nesbitt Seyedeh F.Darghiasi Anamaria G.Zavala Trevor J.Lujan Clare K.Fitzpatrick Mary C.Farach-Carson Danielle Wu Gunes Uzer Mechanical and Biomedical Engineering Department Boise State UniversityUSA Department of Diagnostic and Biomedical Sciences UTHealth Houston School of DentistryUSA Department of Bioengineering Rice UniversityUSA Department of Biosciences Rice UniversityUSA Biomedical Engineering Program Boise State UniversityUSA

Aged individuals and astronauts experience bone loss despite rigorous physical *** mechanoresponse is in-part regulated by mesenchymal stem cells(MSCs)that respond to mechanical *** delivery of low intensity vibration(LIV)recovers MSC proliferation in senescence and simulated microgravity models,indicating that age-related reductions in mechanical signal delivery within bone marrow may contribute to declining bone *** answer this question,we developed a 3D bone marrow analog that controls trabecular geometry,marrow mechanics and external *** finite element(FE)models were developed to quantify strain environment within hydrogels during *** marrow analogs with gyroid-based trabeculae of scaffold volume fractions(SV/TV)corresponding to adult(25%)and aged(13%)mice were printed using polylactic acid(PLA).MSCs encapsulated in migration-permissive hydrogels within printed trabeculae showed robust cell populations on both PLA surface and hydrogel within a *** 14 days of LIV treatment(1 g,100 Hz,1 h/day),cell proliferation,type-I collagen(Collagen-I)and filamentous actin(F-actin)were quantified for the cells in the hydrogel *** LIV increased all measured outcomes,FE models predicted higher von Mises strains for the 13%SV/TV groups(0.2%)when compared to the 25%SV/TV group(0.1%).While LIV increased collagen-I volume 34%more in 13%SV/TV groups when compared to 25%SV/TV groups,collagen-I and F-actin measures remained lower in the 13%SV/TV groups when compared to 25%SV/TV counterparts,indicating that both LIV-induced strains and scaffold volume fraction(*** scaffold surface)affect cell behavior in the hydrogel ***,bone marrow analogs offer a robust and repeatable platform to study bone mechanobiology.

关键词： Mechanical signals Low-intensity vibration Mesenchymal stem cells 3D printing Mechanical modeling Tissue modeling

来源：评论

学校读者我要写书评

暂无评论

Regulation of Leukaemia Associated Rho GEF (LARG/ARHGEF12)

引用

Small GTPases 2022年第1期13卷 196-204页

作者： Ghanem, Neda Z. Matter, Michelle L. Ramos, Joe W. Cancer Biology Program University of Hawaii Cancer Center University of Hawaii at Mānoa Honolulu United States Molecular Biosciences and BioEngineering Graduate Program University of Hawaii at Mānoa Honolulu United States

The Ras homologous (Rho) protein family of GTPases (RhoA, RhoB and RhoC) are the members of the Ras superfamily and regulate cellular processes such as cell migration, proliferation, polarization, adhesion, gene transcription and cytoskeletal structure. Rho GTPases function as molecular switches that cycle between GTP-bound (active state) and GDP-bound (inactive state) forms. Leukaemia-associated RhoGEF (LARG) is a guanine nucleotide exchange factor (GEF) that activates RhoA subfamily GTPases by promoting the exchange of GDP for GTP. LARG is selective for RhoA subfamily GTPases and is an essential regulator of cell migration and invasion. Here, we describe the mechanisms by which LARG is regulated to facilitate the understanding of how LARG mediates functions like cell motility and to provide insight for better therapeutic targeting of these functions. © 2021 Informa UK Limited, trading as Taylor & Francis Group.

关键词： ARHGEF12 GTPase LARG migration Rho RhoGef signal transduction

来源：评论

学校读者我要写书评

暂无评论

Synthesis of Flexible Cellulose Based Bioelectrode for Neural Sensing Applications

Synthesis of Flexible Cellulose Based Bioelectrode for Neura...

引用

2024 Advances in Science and Engineering Technology International Conferences, ASET 2024

作者： Alex, Meera Ali, Amaal Abdulraqeb Al Othman, Amani Al Nashash, Hasan Al-Sayah, Mohammed H. Biosciences and Bioengineering Program American University of Sharjah Sharjah United Arab Emirates Chemical and Biological Engineering Deapartment American University of Sharjah Sharjah United Arab Emirates American University of Sharjah Electrical Engineering Deapartment Sharjah United Arab Emirates American University of Sharjah Biology Chemistry and Environmental Sciences Deapartment Sharjah United Arab Emirates

ISBN: (纸本)9798350344134

Bioelectrodes act as pivotal interfaces between soft neural tissues and external devices for sensing or stimulating purposes. Conventionally, metals such as platinum and iridium have been utilized as bioelectrode materials. However, challenges in interfacing, design and implementation led to the exploration of alternative materials. These challenges include: biocompatibility, mechanical mismatches between tissues and electrodes, cost, reliability, durability in body fluids, and sustainability. This study proposes to synthesize and characterize natural-based cellulose bioelectrodes as flexible, viable, cost-effective, and sustainable substitute for conventional metal-based bioelectrodes, particularly in neural/muscle sensing applications. We report the synthesis of a composite cellulosebased bioelectrode using Polydimethylsiloxane (PDMS) and glycerol at varying compositions. The fabricated electrode was characterized electrochemically to determine its bulk impedance, conductivity, and charge storage density. Results show that the cellulose-based biomaterial containing 23% cellulose, 7% glycerol, and 70% PDMS exhibits a bulk impedance of 0.732 kΩ and a conductivity of 1.8×10-3 S/cm. The flexible electrode could store a charge of 0.589 mC/cm2 which is lower than the safe limit for some tissues. © 2024 IEEE.

关键词： Tissue

来源：评论

学校读者我要写书评

暂无评论

MULTIPLEXED DNA-DIRECTED PATTERNING OF ANTIBODIES FOR SINGLE-CELL SURFACE MARKER ANALYSIS 25

MULTIPLEXED DNA-DIRECTED PATTERNING OF ANTIBODIES FOR SINGLE...

引用

25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021

作者： Kozminsky, Molly Scheideler, Olivia J. Li, Brian Liu, Nathaniel K. Sohn, Lydia L. California Institute of Quantitative Biosciences Berkeley United States UC Berkeley-UCSF Graduate Program in Bioengineering Berkeley and San Francisco United States UC Berkeley Department of Mechanical Engineering Berkeley United States

ISBN: (纸本)9781733419031

Cell surface marker expression is a ubiquitously measured property of single-cell phenotypes. However, measuring several markers with exogenous labels can be costly and complex. We applied a novel bioconjugation chemistry known as DNA-directed patterning to functionalize glass substrates with great specificity and flexibility. Combined with a newly-designed node-pore sensing device, we have developed a flexible and label-free platform for multiplexed surface-marker screening of single cells. In this work, we present our design and demonstrate our platform's capability to screen two example cell types for surface-marker expression. © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.

关键词： DNA

来源：评论

学校读者我要写书评

暂无评论

Development of a tRNA-derived small RNA diagnostic and prognostic signature in liver cancer

引用

Genes & Diseases 2022年第2期9卷 393-400页

作者： Yi Zuo Shaoqiu Chen Lingling Yan Ling Hu Scott Bowler Emory Zitello Gang Huang Youping Deng Tianyou Hospital Affiliated to Wuhan University of Science and TechnologyWuhan 430064PR China Department of Quantitative Health Sciences John A.Burns School of MedicineUniversity of Hawaii at ManoaHonoluluHI 96813USA Molecular Biosciences and Bioengineering Program College of Tropical Agriculture and Human ResourcesUniversity of Hawaii at ManoaHonoluluHI 96822USA Shanghai Key Laboratory for Molecular Imaging Shanghai University of Medicine and Health SciencesShanghai 201318PR China

Liver cancer presents divergent clinical *** remain opportunities for molecular markers to improve liver cancer diagnosis and prognosis,especially since tRNA-derived small RNAs(tsRNA)have rarely been *** this study,a random forests(RF)diagnostic model was built based upon tsRNA profiling of paired tumor and adjacent normal samples and validated by independent validation(IV).A LASSO model was used to developed a seven-tsRNA-based risk score signature for liver cancer *** performance was evaluated by a receiver operating characteristic curve(ROC curve)and Precision-Recall curve(PR curve).The five-tsRNA-based RF diagnosis model had area under the receiver operating characteristic curve(AUROC)88%and area under the precision–recall curve(AUPR)87%in the discovery cohort and 87%and 86%in IV-AUROC and IV-AUPR,*** seven-tsRNA-based prognostic model predicts the overall survival of liver cancer patients(Hazard Ratio 2.02,95%CI 1.36–3.00,P<0.001),independent of standard clinicopathological prognostic ***,the model successfully categorizes patients into high-low risk *** and prognostic modeling can be reliably utilized in the diagnosis of liver cancer and high-low risk classification of patients based upon tsRNA characterization.

关键词： Diagnosis Liver cancer Prognosis Random forests tRNA-derived small RNAs

来源：评论

学校读者我要写书评

暂无评论

GranatumX:A Community-engaging, Modularized, and Flexible Webtool for Single-cell Data Analysis

引用

Genomics, Proteomics & Bioinformatics 2021年第3期19卷 452-460页

作者： David G.Garmire Xun Zhu Aravind Mantravadi Qianhui Huang Breck Yunits Yu Liu Thomas Wolfgruber Olivier Poirion Tianying Zhao Cédric Arisdakessian Stefan Stanojevic Lana X.Garmire Department of Electrical Engineering&Computer Science University of MichiganAnn ArborMI 48109USA Epidemiology Program University of Hawaii Cancer CenterHonoluluHI 96813USA Department of Biostatistics University of MichiganAnn ArborMI 48109USA Department of Computational Medicine and Bioinformatics University of MichiganAnn ArborMI 48105USA Molecular Biosciences and Bioengineering Graduate Program University of Hawaii at ManoaHonoluluHI 96822USA

We present GranatumX,a next-generation software environment for single-cell RNA sequencing(scRNA-seq)data *** is inspired by the interactive webtool *** enables biologists to access the latest scRNA-seq bioinformatics methods in a web-based graphical *** also offers software developers the opportunity to rapidly promote their own tools with others in customizable *** architecture of GranatumX allows for easy inclusion of plugin modules,named Gboxes,which wrap around bioinformatics tools written in various programming languages and on various *** can be run on the cloud or private servers and generate reproducible *** is a community-engaging,flexible,and evolving software ecosystem for scRNA-seq analysis,connecting developers with bench *** is freely accessible at http://***/granatumx/app.

关键词： Single-cell RNA sequencing Analysis Pipeline Webtool Module

来源：评论

学校读者我要写书评

暂无评论

Synthesis of Flexible Cellulose Based Bioelectrode for Neural Sensing Applications

Synthesis of Flexible Cellulose Based Bioelectrode for Neura...

引用

Advances in Science and Engineering Technology International Conferences (ASET)

作者： Meera Alex Amaal Abdulraqeb Ali Amani Al Othman Hasan Al Nashash Mohammed H. Al-Sayah Biosciences and Bioengineering Program American University of Sharjah Sharjah UAE Chemical and Biological Engineering Deapartment American University of Sharjah Sharjah UAE Electrical Engineering Deapartment American University of Sharjah Sharjah UAE Biology Chemistry and Environmental Sciences Deapartment American University of Sharjah Sharjah UAE

ISBN: (数字)9798350344134

ISBN: (纸本)9798350344141

Bioelectrodes act as pivotal interfaces between soft neural tissues and external devices for sensing or stimulating purposes. Conventionally, metals such as platinum and iridium have been utilized as bioelectrode materials. However, challenges in interfacing, design and implementation led to the exploration of alternative materials. These challenges include: biocompatibility, mechanical mismatches between tissues and electrodes, cost, reliability, durability in body fluids, and sustainability. This study proposes to synthesize and characterize natural-based cellulose bioelectrodes as flexible, viable, cost-effective, and sustainable substitute for conventional metal-based bioelectrodes, particularly in neural/muscle sensing applications. We report the synthesis of a composite cellulosebased bioelectrode using Polydimethylsiloxane (PDMS) and glycerol at varying compositions. The fabricated electrode was characterized electrochemically to determine its bulk impedance, conductivity, and charge storage density. Results show that the cellulose-based biomaterial containing 23% cellulose, 7% glycerol, and 70% PDMS exhibits a bulk impedance of 0.732 kΩ and a conductivity of 1.8×10 −3 S/cm. The flexible electrode could store a charge of 0.589 mC/cm 2 which is lower than the safe limit for some tissues.

关键词： Electrodes Costs Platinum Cellulose Conductivity Muscles Sensors Impedance Reliability Sustainable development

来源：评论

学校读者我要写书评

暂无评论

Spontaneous and Induced Oscillations in Confined Epithelia

arXiv

引用

arXiv 2024年

作者： Parmar, Toshi Dow, Liam P. Pruitt, Beth L. Cristina Marchetti, M. Department of Physics University of California Santa Barbara Santa BarbaraCA93106 United States Interdisciplinary Program in Quantitative Biosciences University of California Santa Barbara Santa BarbaraCA93106 United States Departments of Bioengineering and of Mechanical Engineering University of California Santa Barbara Santa BarbaraCA93106 United States

The feedback between mechanical and chemical signals plays a key role in controlling many biological processes and collective cell behavior. Here we focus on the emergence of spatiotemporal density waves in a one-dimensional"cell train." Combining a minimal theoretical model with in vitro experiments on MDCK epithelial cells confined to a linear pattern, we examine the spontaneous oscillations driven by feedback between myosin activation and mechanical deformations, as well as their effect on the response of the tissue to externally applied deformations. We show that the nature and frequency of spontaneous oscillations is controlled by the size of the cell train, with a transition from size-dependent standing waves to intrinsic spontaneous waves at the natural frequency of the tissue. The response to external boundary perturbations exhibit a resonance at this natural frequency, providing a possible venue for inferring the mechanochemical couplings that control the tissue behavior from rheological experiments. Copyright © 2024, The Authors. All rights reserved.

关键词： Tissue

来源：评论

学校读者我要写书评

暂无评论

Advances in liposomal nanotechnology: from concept to clinics

RSC Pharmaceutics

引用

RSC Pharmaceutics 2024年第5期1卷 928-948页

作者： Reem M. Senjab Nour AlSawaftah Waad H. Abuwatfa Ghaleb A. Husseini Biomedical Engineering Program College of Engineering American University of Sharjah Sharjah P.O. Box 26666 United Arab Emirates ghusseini@aus.edu Materials Science and Engineering Ph.D. Program College of Arts and Sciences American University of Sharjah Sharjah P.O. Box 26666 United Arab Emirates Department of Chemical and Biological Engineering College of Engineering American University of Sharjah Sharjah P.O. Box 26666 United Arab Emirates Biosciences and Bioengineering PhD Program College of Engineering American University of Sharjah Sharjah P.O. Box 26666 United Arab Emirates

Liposomes, spherical phospholipid vesicles with a unique morphology mimicking that of body cells, have emerged as versatile nanoparticles for drug delivery. Their biocompatibility, low cytotoxicity, targeted delivery, and hydrophobic and hydrophilic characteristics make them stand out over traditional drug delivery systems. Liposomes can be tailored in size, composition, lamellarity, and surface charge, offering a unique level of customization for various applications. Extensive research in liposome technology has led to the development of a wide range of liposomal formulations with enhanced functionalities, such as PEGylated liposomes, ligand-targeted liposomes, and stimuli-responsive liposomes. Beyond their crucial role in cancer treatment, liposomes play a significant role in influenza, COVID-19, cancer, and hepatitis A vaccines. They are also utilized in pain management, fungal treatment, brain targeting, and topical and ocular drug delivery. This review offers insight into the types of liposomes, their composition, preparation methods, characterization methods, and clinical applications. Additionally, it discusses challenges and highlights potential future directions in liposome-based drug delivery.

关键词：

来源：评论

学校读者我要写书评

暂无评论

Extracellular respiration is a latent energy metabolism in Escherichia coli

引用

Cell 2025年第11期188卷 2907-2924.e23页

作者： Kundu, Biki Bapi Krishnan, Jayanth Szubin, Richard Patel, Arjun Palsson, Bernhard O. Zielinski, Daniel C. Ajo-Franklin, Caroline M. PhD Program in Systems Synthetic and Physical Biology Rice University Houston 77005 TX United States Department of Bioengineering University of California San Diego La Jolla 92093 CA United States Novo Nordisk Foundation Center for Biosustainability Technical University of Denmark Kemitorvet Building 220 Kgs. Lyngby 2800 Denmark Departments of Biosciences Bioengineering Chemical and Biomolecular Engineering and The Rice Synthetic Biology Institute Rice University Houston 77005 TX United States

Diverse microbes utilize redox shuttles to exchange electrons with their environment through mediated extracellular electron transfer (EET), supporting anaerobic survival. Although mediated EET has been leveraged for bioelectrocatalysis for decades, fundamental questions remain about how these redox shuttles are reduced within cells and their role in cellular bioenergetics. Here, we integrate genome editing, electrochemistry, and systems biology to investigate the mechanism and bioenergetics of mediated EET in Escherichia coli, elusive for over two decades. In the absence of alternative electron sinks, the redox cycling of 2-hydroxy-1,4-naphthoquinone (HNQ) via the cytoplasmic nitroreductases NfsB and NfsA enables E. coli respiration on an extracellular electrode. E. coli also exhibits rapid genetic adaptation in the outer membrane porin OmpC, enhancing HNQ-mediated EET levels coupled to growth. This work demonstrates that E. coli can grow independently of classic electron transport chains and fermentation, unveiling a potentially widespread new type of anaerobic energy metabolism. © 2025 Elsevier Inc.

关键词： adaptive laboratory evolution anaerobic metabolism electron shuttles extracellular electron transfer flux balance analysis iModulon analysis nitroreductases redox homeostasis

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：