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The association between the genetic polymorphisms of LMP2/LMP7 and the outcomes of HCV infection among drug users

The Journal of Biomedical Research 2010年第5期24卷 374-380页

作者： Qian Cui Yongxiang Zhang Jing Su Chao Shi Na Lei Keqin Ding Jun Li Rongbin Yu L u Wang Ning Wang Department of Epidemiology and Biostatistics School of Public HealthNanjing Medical UniversityNanjing 210029JiangsuP rovinceChina Department of Infectious Diseases the First Affiliated Hospital of Nanjing Medical UniversityNanjing 210029Jiangsu Provi nceChina Department of Epidemiology National Center for AIDS/STD Control and ProventionChinese Center for Disease Control and PreventionBeijing 100050China

Objective：To investigate a possible association of LMP2/LMP7 genes with chronic hepatitis C virus（HCV） infection,and to assess whether LMP2/LMP7 genes could influence the outcomes of HCV infection among drug ***：Genomic DNAs of 362 anti-HCV sero-positive drug users and 225 control drug users were extracted from the peripheral blood *** sero-positive patients were divided into those who had persistent infection and those who had spontaneously cleared the *** of LMP genes were determined by PCR combined with restriction fragment length polymorphism（RFLP）.Results：The distribution of LMP2 genotypes among the control,persistent infection and spontaneous clearance groups were not ***,the LMP7 codon 145 Gln/Lys,Lys/Lys,and Gln/Lys＋Lys/Lys genotypes were found significantly more frequent in the persistent infection group than in control group（OR=1.75,95%CI=1.06～2.90;OR=3.16,95%CI=1.23-8.12;OR=1.94,95%CI=1.21-3.12,respectively）.Similarly,the frequencies of the codon 145 Gln/Lys,Lys/Lys,and Gln/Lys＋Lys/Lys genotypes were found significantly more frequent in the persistent infection group than in the spontaneous clearance group（OR=1.64,95%CI=1.04-2.57;OR=2.40,95%CI=1.09-5.28;OR=1.76,95%CI=1.152.69,respectively）.Stratified analysis indicated that combined genotype Gln/Lys＋Lys/Lys of the LMP7 gene was related to an increasing susceptibility to HCV infection（OR=1.91,95%CI=1.02-3.55;OR=2.19,95%CI=1.243.89;OR=1.91,95%CI=1.05-3.48,OR=2.86,95%CI=1.41-5.78,respectively）and the risk of persistent HCV infection（OR=1.94,95%CI=1.12-3.34;OR=2.02,95%CI=1.21-3.38;OR=1.78,95%CI=1.12-2.85,OR=2.23,95%CI=1.09-4.58,respectively）among30-year-old,males,the injection drug user（IDU）subjects and/or the shorter duration drug users（≤5 y）.Conclusion：These results suggest that polymorphism of the LMP7 gene may have an influence on the outcomes of HCV infection,and is one of the factors accounting for the genetic susceptibility to HCV infection among d

关键词： hepatitis C virus LMP gene polymorphism infection outcome

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Improving China＇s antiretroviral treatment program： assessing current and future performance using the principals of ethics

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Chinese Medical Journal 2009年第11期122卷 1346-1351页

作者： YIN Wen-yuan ZHANG Fu-jiet Naomi Juniper WU Zun-you Division of Health Education and Behavioral Intervention National Centre for AIDS/STD Control and Prevention Chinese Center for Disease Control and Prevention Beijing 100050 China Division of Treatment and Care National Centre for AIDS/STD Control and Prevention Chinese Center for Disease Control and Prevention Beijing 100050 China

The global commitment to providing antiretroviral therapy （ART） to people living with human immunodeficiency virus/acquired immunodeficiency syndrome （HIV/aids） in low-income countries has raised hope that the increasing momentum in the fight against the worldwide HIV/aids pandemic will be sufficient to control it. However, improved availability of subsidized antiretroviral （ARV） treatments in low-income .countries raises complex ethical issues. In many resource-constrained countries the number of individuals infected with HIV in need of treatment far exceeds the supply of ARV medication. Resource allocation decisions can be made on the basis of many epidemiological, ethical, or preferential treatment priority criteria. Healthcare systems and funding in low-income countries are limited, requiring a step-by-step approach to scaling- up programs to reach their stated aims.

关键词： acquired immunodeficiency syndrome antiretroviral treatment program ethics

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Construction and characterization of a new simian/human immunodeficiency viruses clone carrying an env gene derived from a CRF07_BC strain

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Chinese Medical Journal 2009年第23期122卷 2874-2879页

作者： LI Yue YANG Gui-bo CHEN Qi-min LIU Qiang MENG Zhe-feng GENG Yun-qi QIAO Wen-tao SHAO Yi-ming College of Lifesciences Nankai University Tianjin 300071 China National Center for AIDS/STD Control and Prevention Chinese Center for Disease Control and Prevention Beijing 100050 China

Background The CRF07_BC recombinant strain has been one of the most predominantly circulated HIV-1 strains in China, it is therefore necessary and urgent to develop a relevant animal model to evaluate candidate vaccines targeting HIV-1 CRF07 BC. A highly replication-competent simian/human immunodeficiency viruses （SHIV） construct containing the Chinese CRF07_BC HIV-1 env gene with the ability to infect Chinese rhesus monkeys would serve as an important tool in the development of HIV vaccines. The aim of this study was to examine whether SHIV XJDC6431 with the env fragment from a Chinese HIV-1 isolate virus could infect the human and monkey peripheral blood mononuclear cell （PBMC）, establish infection in Chinese rhesus macaque. Methods A SHIV strain was constructed by replacing the rev/env genes of SHIV KB9 with the corresponding fragment derived from the HIV-1 CRF07_BC strain. The infectious activity of the SHIV clones was determined in vitro in PBMCs from both non-human primate animals and humans. Finally, one Chinese rhesus macaques （Macaca mulatta） was infected with one SHIV via intravenous infusion. Results One SHIV clone designated as SHIV XJDC6431, was generated that could infect macaque and human PBMC. The virus produced from this clone also efficiently infected the CCR5-expressing GHOST cell lines, indicating that it uses CCR5 as its coreceptor. Finally, the virus was intravenously inoculated into one Chinese rhesus macaque. Eventually, the animal became infected as shown by the occurrence of viremia within 3 of infection. The viral load reached 105 copies of viral RNA per ml of plasma during the acute phase of infection and lasted for 10 weeks post infection. Conclusions We conclude that SHIV XJDC6431 is an R5-tropic chimeric virus, which can establish infection not only in vitro but also in vivo in the Chinese rhesus macaque. Although the animal inoculated with SHIV XJDC6431 became infected without developing a pathologic phenotype, the virus efficiently r

关键词： simian/human immunodeficiency viruses human immunodeficiency virus type 1 CRFO7_BC vaccine

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Commentary: Socio-economic determinants of tuberculosis in Recife, Brazil

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INTERNATIONAL JOURNAL OF EPIDEMIOLOGY 2009年第5期38卷 1295-1296页

作者： Marks, Suzanne Clinical and Health Systems Research Branch Division of Tuberculosis Elimination National Center for HIV/AIDS Viral Hepatitis STD and TB Prevention US Centers for Disease Control and Prevention. smarks@cdc.gov

The article examines the study 'Is it better to be rich in a poor area or poor in a rich area? A multilevel analysis of a case-control study of social determinants of tuberculosis,' by X. Ricardo Arraes de Alencar, V. S. Wayner and colleagues, published in the 2009 issue. It notes the model used by the authors to identify least ownership of goods or little personal wealth as being most linked tuberculosis (TB) in Recife, Brazil. The limitations of the study such as selection bias are also discussed.

关键词： TUBERCULOSIS POOR people SOCIAL status WEALTH PERSONAL property

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Complete Human Immunodeficiency Virus-1 Specific T Lymphocyte Response to Chinese Human Immunodeficiency Virus-1 B/C Chronic Infectors

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Biomedical and Environmental Sciences 2009年第6期22卷 522-528页

作者： ZHE WANG HONG-WEI LIU KUN-XUE HONG ZU-JIANG YU JIAN -PING CHEN YU-HUA RUAN QUAN-CHENG KAN YI-MING SHAO The First Affiliated Hospital of Zhengzhou University Zhengzhou 450052 Henan China Center for AIDS/STD Control and Prevention Henan Center for Disease Control and Prevention Zhengzhou 450016 Henan China Division of Virology and Immunology National Center for Disease Control and Prevention Chinese Center for Disease Control and Prevention Beijing 100052 China

Objective To characterize the human immunodeficiency virus （HIV） -specific T lymphocyte responses and identify the immunodominant regions in Chinese HIV-1 recombinant subtype B/C chronic infectors at complete genome level. Methods Twenty-five HIV-I B/C recombinant chronic infectors were screened for their specific T lymphocyte responses to a panel of peptides corresponding to the complete HIV-1 subtype B genome by gamma interferon ELISPOT assay. Kruskal-Wallis nonparametric analysis of variance was used to test significant differences across gene regions, and Tukey pairwise analysis was used to identify differences between gene regions. Spearman rank correlation was used to assess the relation between responses. Results The order of recognized frequencies of specific T lymphocyte responses to HIV proteins was Nef〉Vpr〉Gag〉Pol〉Vpu〉Env〉Rev〉Vif〉Tat. When adjusted for protein length, Nef, Vpr, Gag, and Pol were the most intensely targeted proteins and the central region of Nef, Gag p24, Pol RT, and Vpr was most frequently recognized. No significant correlation was observed between the magnitude of IFN-γ production of HIV-l-specific T lymphocyte responses and plasma viremia, breadth of response and CD4 counts. Conclusion The central region of Nef, Gag p24, Pol RT, and Vpr is most frequently targeted in HIV-1 B/C recombinants chronic infectors. HIV-1-specific T lymphocyte responses and plasma viremia or CD4 counts play no protective role at complete genome level in these infectors.

关键词： Human immunodeficiency virus （HIV） Specific T lymphocyte response Elispot IFN-γ

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A mouse model based on replication-competent Tiantan vaccinia expressing luciferase/HIV-1 Gag fusion protein for the evaluation of protective efficacy of HIV vaccine

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Chinese Medical Journal 2009年第14期122卷 1655-1659页

作者： HUANG Yang QIU Chao LIU Lian-xing FENG Yan-meng ZHU Ting XU Jian-qing State Key Laboratory for Infectious Diseases Prevention and Control National Center for AIDS/STD Control and Prevention Chinese Center of Disease Control and Prevention Beijing 100050 China Shanghai Public Health Clinical Center Institutes of Biomedical Sciences Fudan University Shanghai 201508 China

Background Developing an effective vaccine against human immunodeficiency virus type 1 （HIV-1） remains a grand challenge after more than two decades of intensive effort. It is partially due to the lack of suitable animal models for screening and prioritizing vaccine candidates. In this study, we aim to develop a mice model to test HIV-1 vaccine efficacy. Methods We constructed a recombinant vaccinia expressing firefly luciferase and HIV-1 Gag fusion protein based on Tiantan strain, an attenuated but replication-competent poxvirus （rTTV-lucgag）. By quantifying the luciferase activity as its read out, we defined the biodistribution of Tiantan strain poxvirus in mice inoculated intraperitoneally and attempted to apply this model to evaluate the HIV-1 vaccine efficacy. Results Our data demonstrated that the rTTV-lucgag was able to express high level of luciferase （〈106 relative luciferase units （RLU）/mg protein） and HIV-1 Gag （〉3 folds increase comparing to the control）. After intraperitoneal inoculation, this virus had dominant replication in the ovary, uterus, and cervix of mice and the luciferase activities in those organs are significantly correlated with viral titers （r^2=0.71, P 〈0.01）. Pre-immunization with an HIV gag DNA vaccine reduced the luciferase activity in ovary from （6006±3141） RLU/mg protein in control group to （1538±463） RLU/mg protein in vaccine group （P=0.1969）. Conclusions The luciferase activity in ovary could represent viral replication in vivo; this rTTV-lucgag/mice model may be suitable to assess the protective efficacy of cytotoxic T-cell responses to HIV Gag with less tedious work and high through-put.

关键词： human immunodeficiency virus type 1 vaccine mice model luciferase

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Ps-02001 The treatment outcome of 106 HIV-positive patients with tuberculosis

Ps-02001 The treatment outcome of 106 HIV-positive patients ...

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第二届国际防痨和肺部疾病联合会亚太区学术大会暨2009年中国防痨协会学术会议

作者： Y J Lai N Wang S M Cheng L Zhou X J Wang National Center for TB Control and Prevention China CDC Beijing National Center for AIDS/STD Control and Prevention China CDC Beijing

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OP-S061005 TB surveillance in HIV/aids

OP-S061005 TB surveillance in HIV/AIDS

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第二届国际防痨和肺部疾病联合会亚太区学术大会暨2009年中国防痨协会学术会议

作者： Y J Lai N Wang S M Cheng L Zhou X J Wang National Center for AIDS/STD Control and Prevention China CDC Beijing National Center for TB Control and Prevention China CDC Beijing

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Human immunodeficiency virus and hepatitis C virus co-infection： epidemiology, natural history and the situation in China

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Chinese Medical Journal 2009年第1期122卷 93-97页

作者： LU Ying Matthew Robinson ZHANG Fu-jie Department of Public Health Lanzhou University Lanzhou Gansu 730000 China School of Medicine University of North Carolina Chapel Hill 27599 USA National Center for AIDS/STD Control and Prevention Chinese Center for Disease Control and Prevention Beijing 100050 China

Worldwide, the hepatitis C vires （HCV） and human immunodeficiency virus （HIV） pandemics poseserious threats to global health, currently infecting 130 million and 40 million people respectively. Of those infected with HW, an estimated 4-12 million are co-infected with HCV. Due to shared risk factors for transmission, co-infection with HIV and HCV is common in China. Liver disease was the second leading cause of death behind acquired imunodeficiency syndrome （aids） in co-infected patients. Several studies have confirmed that HIV co-infection accelerates the clinical course of chronic HCV infection and leads to an increased risk of cirrhosis, hepatocellular carcinoma, and decompensated liver disease.

关键词： human immunodeficiency virus hepatitis C virus co-infection

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Potential protective role of HLA-A*03 in HIV-1 Infection and disease progression in a Chinese Former Blood Donor cohort

Potential protective role of HLA-A*03 in HIV-1 Infection and...

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21st IUBMB and 12th FAOBMB International Congress of Biochemistry and Molecular Biology

作者： Weilun Zhang Peng Zhang Li Wang Kunxue Hong Yanxin Liu Bing Su Cheng Xu Jianqin Xu Yuhua Ruan Yimin Shao Dexian Zheng Institute of Basic Medical Sciences Peking Union Medical College National Center for AIDS/STD Control and Prevention Anhui Center for Disease Control and Prevention Fuyang Center for Disease Control and Prevention

To assess the influence of human leukocyte antigen(HLA) on HIV-1 infection and the in vivo control of virus,294 HIV-positive and 59 HIV-negative former blood donors(FBDs) in Fuyang City of Anhui province were enrolled to perform HLA genotyping and the association of HIV-1 infection and the allele distribution were *** viral load and CD4/CD8 T cell counts were assessed in all *** observed that the frequency of HLA-A*03

关键词： HLA HIV Potential protective role of HLA-A in HIV-1 Infection and disease progression in a Chinese Former Blood Donor cohort

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