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Pore size dependence of DNA translocation through polymer nanopores fabricated by nanoimprint lithography 21

Pore size dependence of DNA translocation through polymer na...

引用

21st International Conference on Miniaturized systems for Chemistry and Life Sciences, MicroTAS 2017

作者： Choi, Junseo Park, Sunggook Mechanical and Industrial Engineering Department Louisiana State University United States Center of BioModular Multi-Scale Systems for Precision Medicine United States

ISBN: (纸本)9780692941836

We report, for the first time, fabrication of sub-10 nm pores in freestanding polymer membranes via a simple, cost effective, high throughput but still deterministic fabrication method. Nanoimprint lithography (NIL) was combined with a polymer reflow process to build the polymer nanopore membranes in SU-8. The fabricated nanopore membranes were successfully employed for transient current measurements during translocation of DNA molecules through the nanopores. The DNA capture rate increased proportional to the square of the nanopore diameter. © 17CBMS-0001.

关键词： Nanoimprint lithography

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Microfluidic affinity purification of cancer-specific extracellular vesicles 21

Microfluidic affinity purification of cancer-specific extrac...

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21st International Conference on Miniaturized systems for Chemistry and Life Sciences, MicroTAS 2017

作者： Jackson, Joshua M. Witek, Malgorzata A. Pullagurla, Swathi R. Park, Daniel S. Hupert, Mateusz L. Herrera, Kristina Murphy, Michael C. Soper, Steven A. University of Kansas United States Center of BioModular Multi-Scale Systems United States Louisiana State University United States Biofluidica Inc. United States University of North Carolina United States

ISBN: (纸本)9780692941836

Extracellular vesicles (EVs) are nanovesicles (30-500 nm) secreted by cells that protect protein/RNA biomarkers in blood and are key effectors in cancer. However, discerning biologically active subpopulations of cancer-related EVs has proved difficult due to lagging technological developments for EV purification that supersede the "gold standard" differential ultracentrifugation. Herein, we report technologies for the continuous-flow Microfluidic Affinity Purification of EVs (EV-MAP) and EV release for downstream enumeration and molecular profiling. © 17CBMS-0001.

关键词： Diseases

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High flow rate capture of circulating tumor cells using a small footprint polymer device

High flow rate capture of circulating tumor cells using a sm...

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15th International Conference on Miniaturized systems for Chemistry and Life Sciences 2011, MicroTAS 2011

作者： Park, Taehyun Park, Daniel Sang-Won Murphy, Michael C. Department of Mechanical Engineering Center for BioModular Multi-Scale Systems Louisiana State University United States

ISBN: (纸本)9781618395955

High flow rate capture of circulating tumor cells (CTCs) was performed with a high recovery rate. A new concept for rare target cell capture using a micro device was investigated parametrically using numerical simulations. High flow rate device (HFRD) prototypes were fabricated based on the simulation results. The devices were coated with antibodies (anti-EpCAM) after UV modification and amine functionalization. The simulated CTCs (MCF-7 cells) were collected and spiked in ~40% hematocrit solutions of human red blood cells. The polymer high flow rate device captured the CTCs with an 80% average recovery rate at a flow rate of 750 μL/min. Copyright © (2011) by the Chemical and Biological Microsystems Society.

关键词： Tumors

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High flow rate circulating tumor cell capture device

High flow rate circulating tumor cell capture device

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ASME 2011 Summer Bioengineering Conference, SBC 2011

作者： Park, Taehyun Park, Daniel Sangwon Murphy, Michael C. Department of Mechanical Engineering Louisiana State University Baton Rouge LA 70803 United States Center for Biomodular Multi-Scale Systems Louisiana State University Baton Rouge LA 70803 United States

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MODULAR AND POLYMER-BASED FLUIDIC systems FOR DNA ANALYSIS:TRAVERSING THROUGH THE MACRO-TO NANO-DOMAIN INTERFACE

MODULAR AND POLYMER-BASED FLUIDIC SYSTEMS FOR DNA ANALYSIS:T...

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第九届亚太国际微分离分析学术会议暨第一届亚太国际芯片实验室学术会议

作者： Steven A.Soper Michael C.Murphy Dimitris Nikitopoulos Sunggook Park Robin L.McCarley Mateusz Hupert Rattikan Chantiwas Hong Wang Hui-Wei Chen Center for BioModular Multi-Scale Systems Louisiana State University Baton Rouge LA 70803 USA

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A polymer, microfluidic analyzer for mutation detection

A polymer, microfluidic analyzer for mutation detection

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13th International Conference on Miniaturized systems for Chemistry and Life Sciences, MicroTAS 2009

作者： Lee, Tae Yoon Han, Kyudong Nikitopoulos, Dimitris E. Soper, Steven S. Batzer, Mark A. Murphy, Michael C. Center for BioModular Multi-Scale Systems Louisiana State University United States Department of Mechanical Engineering Louisiana State University United States Department of Biological Sciences Louisiana State University United States Department of Chemistry Louisiana State University United States

ISBN: (纸本)9780979806421

Recognition of point mutations in codon 12 of the K-ras gene is useful for the early diagnosis of several types of cancer in humans, including colorectal cancer. A microfluidic analyzer, that can detect low-abundance point mutations in human genomic DNA, was developed using a modular approach. Each functional device, including a microfluidic polymerase chain reaction (PCR) reactor, a passive diffusional micromixer reactor, and a microfluidic ligase detection reaction (LDR) reactor, was designed and fabricated in polycarbonate. Interconnects were developed for the passage of analyte between reactors, to ensure adequate spacing for thermal isolation and to facilitate assembly of these reactors using adhesive bonding to form the PCR/LDR processing system. Using the polymer microfluidic system, LDR products were produced from a mixture of mutant DNA to wild type of 1:200 in about 1 h, about half the time needed to execute the same protocol using conventional instrumentation. © 2009 CBMS.

关键词： Microfluidics

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A multi-function, microfluidic module for mutation detection

A multi-function, microfluidic module for mutation detection

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TRANSDUCERS 2009 - 15th International Conference on Solid-State Sensors, Actuators and Microsystems

作者： Tae, Yoon Lee Han, Kyudong Park, Daniel S. Soper, Steven S. Batzer, Mark A. Nikitopoulos, Dimitris E. Murphy, Michael C. Center for BioModular Multi-Scale Systems Louisiana State University Baton Rouge LA United States Department of Mechanical Engineering Louisiana State University Baton Rouge LA United States Department of Biological Sciences Louisiana State University Baton Rouge LA United States Department of Chemistry Louisiana State University Baton Rouge LA United States

ISBN: (纸本)9781424441938

A multi-function, microfluidic module was designed, fabricated, and used for mutation detection using the ligase detection reaction (LDR) following amplification by the polymerase chain reaction (PCR). The module carried out four processing steps: (1) the PCR to amplify a target region in the K-ras gene of the human genome, which can lead to colorectal cancer;(2) the purification of the PCR products;(3) the mixing of the purified PCR products with an LDR reagent using a passive micromixer;and (4) the LDR to make florescent oligonucleotide products detectable by gel electrophoresis. Single nucleotide polymorphisms in the K-ras gene were detected from a concentration ratio of mutant to normal genomic DNA of 1:40 using the multi-function, microfluidic module. ©2009 IEEE.

关键词： Microfluidics

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A multi-function, microfluidic module for mutation detection

A multi-function, microfluidic module for mutation detection

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International Conference on Solid State Sensors and Actuators (TRANSDUCERS)

作者： Tae Yoon Lee Kyudong Han Daniel S. Park Steven S. Soper Mark A. Batzer Dimitris E. Nikitopoulos Michael C. Murphy Department of Mechanical Engineering Louisiana State University Baton Rouge LA USA Louisiana State University Paul M Hebert Law Center Baton Rouge LA US Center of BioModular Multi-Scale Systems Louisiana State University Baton Rouge LA USA

A multi-function, microfluidic module was designed, fabricated, and used for mutation detection using the ligase detection reaction (LDR) following amplification by the polymerase chain reaction (PCR). The module carried out four processing steps: (1) the PCR to amplify a target region in the K-ras gene of the human genome, which can lead to colorectal cancer; (2) the purification of the PCR products; (3) the mixing of the purified PCR products with an LDR reagent using a passive micromixer; and (4) the LDR to make florescent oligonucleotide products detectable by gel electrophoresis. Single nucleotide polymorphisms in the K-ras gene were detected from a concentration ratio of mutant to normal genomic DNA of 1:40 using the multi-function, microfluidic module.

关键词： Microfluidics Genetic mutations Genomics Bioinformatics Polymers Humans Purification Cancer detection Electrokinetics DNA

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Electroless deposition of metals on nanopatterns of organosilane sams

Electroless deposition of metals on nanopatterns of organosi...

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Electrodeposition for Energy Applications - 213th Meeting of The Electrochemical Society

作者： Li, J.-R. Gamo, J.C. Department of Chemistry Center for BioModular Multi-Scale Systems Louisiana State University Baton Rouge LA 70803 United States

ISBN: (纸本)9781566776677

Hybrid metal-organic architectures can be fabricated at the nanoscale using guided self-assembly on well-defined organic scaffolds to produce layered nanostructures. We have developed a new chemical approach to fabricate nanopatterns of metal nanostructures via electroless metal deposition of nanopatterned silane thin films. First, a surface with different functionalities is prepared with engineered nanopatterns of adhesive and resistive silanes. Next, the organosilane nanopatterns are used as a foundation for site-selective electroless deposition of metals such as iron oxide. © The Electrochemical Society.

关键词： Silanes

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A compact microfluidic system with an integrated optical system for singlemolecule detection via fluorescence resonance energy transfer for realtime molecular analyses

A compact microfluidic system with an integrated optical sys...

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12th International Conference on Miniaturized systems for Chemistry and Life Sciences, MicroTAS 2008

作者： Emory, Jason M. Peng, Zhiyong Crawford-Drake, Frederick Chen, Pin-Chuan Murphy, Michael C. Soper, Steven A. Department of Chemistry Louisiana State University Baton Rouge LA United States Center for BioModular Multi-Scale Systems Louisiana State University Baton Rouge LA United States Department of Mechanical Engineering Louisiana State University Baton Rouge LA United States

We have designed and built for the first time, a field deployable instrument for the near real-time reporting of molecular signatures using microfluidics for thermal processing of genomic DNA with integrated optics fo... 详细信息

关键词： Microfluidics

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