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Phasor-FSTM:a new paradigm for multicolor super-resolution imaging of living cells based on fluorescence modulation and lifetime multiplexing

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Light(Science & Applications) 2025年第1期14卷 258-270页

作者： Luwei Wang Yue Chen Jiaqing Guo Xiaoyu Weng Wei Yan Jun Song Tong Ye Junle Qu Center for Biomedical Optics and Photonics&College of Physics and Optoelectronic Engineering Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong ProvinceShenzhen UniversityShenzhen518060China The Photonics Center of Shenzhen University Shenzhen UniversityShenzhen518060China Department of Bioengineering CU-MUSC Bioengineering ProgramClemson UniversityCharlestonSouth Carolina29634USA

Multicolor microscopy and super-resolution optical microscopy are two widely used techniques that greatly enhance the ability to distinguish and resolve structures in cellular *** methods have individually transformed cellular imaging by allowing detailed visualization of cellular and subcellular structures,as well as organelle ***,integrating multicolor and super-resolution microscopy into a single method remains challenging due to issues like spectral overlap,crosstalk,photobleaching,phototoxicity,and technical *** challenges arise from the conflicting requirements of using different fluorophores for multicolor labeling and fluorophores with specific properties for super-resolution *** propose a novel multicolor super-resolution imaging method called phasor-based fluorescence spatiotemporal modulation(Phasor-FSTM).This method uses time-resolved detection to acquire spatiotemporal data from encoded photons,employs phasor analysis to simultaneously separate multiple components,and applies fluorescence modulation to create super-resolution ***-FSTM enables the identification of multiple structural components with greater spatial accuracy on an enhanced laser scanning confocal microscope using a single-wavelength *** demonstrate the capabilities of Phasor-FSTM,we performed two-color to four-color super-resolution imaging at a resolution of~λ/5 and observed the interactions of organelles in live cells during continuous imaging for a duration of over 20 *** method stands out for its simplicity and adaptability,seamlessly fitting into existing laser scanning microscopes without requiring multiple laser lines for excitation,which also provides a new avenue for other super-resolution imaging technologies based on different principles to build multi-color imaging systems with the requirement of a lower budget.

关键词： resolution lifetime simplicity

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Effective stabilization of photobleaching of BAC-Si in bismuth-and erbium-codoped optical fibers by 830 nm laser repeated pumping

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Chinese optics Letters 2025年第1期23卷 19-24页

作者： Qi Yang Boyang Hu Jie Gao Hongxin Ding Jialang Huang Yushi Chu Jianzhong Zhang Gang-Ding Peng Key Laboratory of In-fiber Integrated Optics of Ministry of Education College of Physics and Optoelectronic EngineeringHarbin Engineering UniversityHarbin 150001China Key Laboratory of Photonic Materials and Devices Physics for Oceanic Applications Ministry of Industry and Information TechnologyCollege of Physics and Optoelectronic EngineeringHarbin Engineering UniversityHarbin 150001China Fiber Optical Sensing Center for Excellence Yantai Research InstituteHarbin Engineering UniversityYantai 264006China Photonics and Optical Communications School of Electrical Engineering and TelecommunicationsUniversity of New South WalesSydney NSW 2052Australia

The photobleaching(PB)effect of bismuth active center(BAC)-related silicon(BAC-Si)in the bismuth-and erbium-codoped optical fiber(BEDF)under repeated pumping was *** results demonstrated that the irreversible PB could be eliminated after repeated pumping,while the rest of the PB could be decreased and remained stable in the subsequent *** mechanism was also discussed using electron transferring theory and the distribution function concept of activation ***,the results also demonstrated that BAC-Si could further become stable,reversible,and irreversible after PB,and new standards can be established for the BEDF to evaluate its quality.

关键词： bismuth-and erbium-codoped optical fiber photobleaching fiber laser fiber amplifier

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Lensless efficient snapshot hyperspectral imaging using dynamic phase modulation

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photonics Research 2025年第2期13卷 511-526页

作者： CHONG ZHANG XIANGLEI LIU LIZHI WANG SHINING MA YUANJIN ZHENG YUE LIU HUA HUANG YONGTIAN WANG WEITAO SONG Beijing Engineering Research Center of Mixed Reality and Advanced Display School of Optics and Photonics Beijing Institute of Technology Zhengzhou Research Institute Beijing Institute of Technology State Key Laboratory of Radio Frequency Heterogeneous Integration College of Physics and Optoelectronic Engineering Shenzhen University School of Computer Science and Technology Beijing Institute of Technology School of Electrical and Electronic Engineering Nanyang Technological University School of Artificial Intelligence Beijing Normal University

Snapshot hyperspectral imaging based on a diffractive optical element(DOE) is increasingly featured in recent progress in deep optics. Despite remarkable advances in spatial and spectral resolutions, the limitations of current photolithography technology have prevented the fabricated DOE from being designed at ideal heights and with high diffraction efficiency, diminishing the effectiveness of coded imaging and reconstruction accuracy in some bands. Here, we propose, to our knowledge, a new lensless efficient snapshot hyperspectral imaging(LESHI)system that utilizes a liquid-crystal-on-silicon spatial light modulator(LCoS-SLM) to replace the traditionally fabricated DOE, resulting in high modulation levels and reconstruction accuracy. Beyond the single-lens imaging model, the system can leverage the switch ability of LCoS-SLM to implement distributed diffractive optics(DDO)imaging and enhance diffraction efficiency across the full visible spectrum. Using the proposed method, we develop a proof-of-concept prototype with an image resolution of 1920 × 1080 pixels, an effective spatial resolution of 41.74 μm, and a spectral resolution of 10 nm, while improving the average diffraction efficiency from 0.75 to0.91 over the visible wavelength range(400–700 nm). Additionally, LESHI allows the focal length to be adjusted from 50 mm to 100 mm without the need for additional optical components, providing a cost-effective and timesaving solution for real-time on-site debugging. LESHI is the first imaging modality, to the best of our knowledge,to use dynamic diffractive optics and snapshot hyperspectral imaging, offering a completely new approach to computational spectral imaging and deep optics.

关键词： Diffraction efficiency Diffractive optical elements Imaging systems Phase modulation Spatial light modulators Spatial resolution

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Low-power STED nanoscopy based on temporal and spatial modulation

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Nano Research 2022年第4期15卷 3479-3486页

作者： Luwei Wang Yue Chen Yong Guo Weixin Xie Zhigang Yang Xiaoyu Weng Wei Yan JunleQu Center for Biomedical Photonics&College of Physics and Optoelectronic Engineering Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong ProvinceShenzhen 518060China College of Electronics and Information Engineering Shenzhen UniversityShenzhen 518060China

Stimulated emission depletion(STED)nanoscopy enables the visualization of subcellular organelles in unprecedented ***,reducing the power dependency remains one of the greatest challenges for STED imaging in living ***,we propose a new method,called modulated STED,to reduce the demand for depletion power in STED imaging by modulating the information from the temporal and spatial *** this approach,an excitation pulse is followed by a depletion pulse with a longer delay;therefore,the fluorescence decay curve contains both confocal and STED photons in a laser pulse *** time-resolved detection,we can remove residual diffraction-limited signals pixel by pixel from STED photons by taking the weighted difference of the depleted ***,fluorescence emission in the periphery of an excitation spot is further inhibited through spatial modulation of fluorescent signals,which replaced the increase of the depletion power in conventional *** demonstrate that the modulated STED method can achieve a resolution of<100 nm in both fixed and living cells with a depletion power that is dozens of times lower than that of conventional STED,therefore,it is very suitable for long-term superresolution imaging of living ***,the idea of the method could open up a new avenue to the implementation of other experiments,such as light-sheet imaging,multicolor and three-demensional(3D)super-resolution imaging.

关键词： fluorescence microscopy super-resolution imaging stimulated emission depletion fluorescence lifetime signal modulation

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High-speed readout for direct light orbital angular momentum photodetector via photoelastic modulation

arXiv

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arXiv 2025年

作者： Yang, Dehong Xu, Chang Lai, Jiawei Fan, Zipu Liang, Delang Wang, Shiyu Cheng, Jinluo Sun, Dong International Center for Quantum Materials School of Physics Peking University Beijing China Ministry of Education Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Matter Shaanxi Province Key Laboratory of Quantum Information and Quantum Optoelectronic Devices School of Physics Xi'an Jiaotong University Xi’an China Key Laboratory for Micro-Nano Physics and Technology of Hunan Province Hunan Institute of Optoelectronic Integration College of Materials Science and Engineering Hunan University Changsha China GPL Photonics Laboratory State Key Laboratory of Luminescence Science and Technology Changchun Institute of Optics Fine Mechanics and Physics Chinese Academy of Sciences Changchun China Collaborative Innovation Center of Quantum Matter Beijing China Frontiers Science Center for Nano-optoelectronics School of Physics Beijing China

Recent progress in direct photodetection of light orbital angular momentum (OAM) based on the orbital photogalvanic effect (OPGE) provides an effective way for on-chip direct electric readout of orbital angular momentum, as well as large-scale integration focal-plane array devices. However, the recognition of OAM order from photocurrent response requires the extraction of circular polarization-dependent response. To date, the operation speed of such detector is currently at the minute level and is limited by slow mechanical polarization modulation and low OAM recognition capability. In this work, we demonstrate that the operation speed can be greatly improved via electrical polarization modulation strategy with photoelasitc modulator accompanied by phase-locked readout approach with lock-in amplifier. We demonstrate an operation speed of up to 1 kHz with this new technology in the mid-infrared region (4 μm) on an OAM detector using multilayer graphene (MLG) as photosensitive material. In principle, with new modulation and readout scheme, we can potentially increase the operation speed to 50.14 kHz with a PEM that operates at a state-of-the-art speed. Our work paves the way toward high-speed operation of direct OAM detection devices based on OPGE effect and pushes such technology to a more practical stage for focal plane array applications. Copyright © 2025, The Authors. All rights reserved.

关键词： Photodetectors

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Observations of intracellular second-harmonic generation imaging in black phosphorus nanosheets

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Journal of Innovative Optical Health Sciences 2021年第2期4卷 11-18页

作者： Xiao Peng Yiwan Song Zheng Peng Kaixuan Nie Hao Liu Yingxin Zhou Feifan Zhou Yufeng Yuan Jun Song Junle Qu Center for Biomedical Optics and Photonics(CBOP)&College of Physics and Optoelectronic Engineering Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province Shenzhen University Shenzhen 518060P.R.China

Functionalized black phosphorus(BP)nanosheets have been considered as promising nanoagents in cancer therapy due to their excellent photothermal conversion ***,it is still difficult to visually monitor the dynamic localization of BP nanoagents in cancer *** this paper,we systematically studied the second-harmonic generation(SHG)signals originating from exfoliated BP ***,under the excitation of a high frequency pulsed laser at 950 nm,the SHG signals of BP nanosheets in vitro are almost undetectable because of their poor ***,the intracellular SHG signals from BP nanosheets could be measured by in vivo optical imaging due to the efficient enrichment of living HeLa ***,the SHG signal intensity from BP nanosheets increases with the prolonged incubation *** can be expected that the BP nanosheets could be a promising intracellular SHG nanoprobe employed for visually in vivo biomedical imaging in practical cancer photothermal therapy(PIT).

关键词： Second harmonic generation nanoprobe black phosphorus nanosheets in vivo im-aging HeLa cells visual monitoring

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Intermolecular hydrogen-bonding as a robust tool toward significantly improving the photothermal conversion efficiency of a NIR-II squaraine dye

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Chinese Chemical Letters 2024年第4期35卷 310-315页

作者： Songtao Cai Liuying Wu Yuan Li Soham Samanta Jinying Wang Bing Liu Feihu Wu Kaitao Lai Yingchao Liu Junle Qu Zhigang Yang Center for Biomedical Photonics&Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province College of Physics and Optoelectronic EngineeringShenzhen UniversityShenzhen 518060China

The photothermal therapy(PTT) has come across as a promising noninvasive therapeutic strategy for tumor treatment. However, low photothermal conversion efficiency(PCE) and hydrophobicity may impede the therapeutic efficacy of organic photothermal agents and an efficient PTT-agent must overcome these two major challenges. In this work, we developed a new strategy to promote higher PCE wherein the intermolecular hydrogen-bonding interaction between the single dye molecule and water facilitated the transformation of the absorbed energy into the heat. A hydrophilic squaraine dye(SCy1) with the second near-infrared region(NIR-II) absorption and extremely low emission were designed to exhibit much higher PCE than that of the analogues of pentamethine-dyes(PCy1, PCy2). The presence of the ‘–O-' at middle of squaric cycle enabled the intermolecular H-bonding formation between the SCy1 and water to promote the energy dissipation channel. Moreover, the introduction of long-chain phenylsulfonate groups helped in to improve the water solubility apart from serving as an additional means of further enhancing PCE through fluorescence quenching. Therefore, SCy1 with a squaraine backbone and long-chain sulfonate moieties revealed outstanding photothermal stability and anti-aggregation activity apart from showing exceptionally high PCE(74%) in water. SCy1 demonstrated excellent therapeutic efficacy when applied in the PTT treatment of tumor-bearing mice under a laser irradiation of 915 nm.

关键词： Squaraine-based cyanine Hydrogen bonds High PCE Anti-aggregation Photothermal therapy

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Shedding light on biology and healthcare--preface to the special issue on biomedical optics

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Light(Science & Applications) 2022年第7期11卷 1315-1317页

作者： Peng Xi Xunbin Wei Junle Qu Valery V.Tuchin Department of Biomedical Engineering College of Future TechnologyPeking University100871BeijingChina Department of Biomedical Engineering Peking University100081BeijingChina Center for Biomedical Optics and Photonics(CBOP)&College of Physics and Optoelectronic Engineering Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province and Ministry of EducationShenzhen University518060ShenzhenChina Saratov State University 83 Astrakhanskaya str.Saratov410012Russia

During the past decade,there has been an explosion in the development of novel optical techniques in biomedical research and clinical *** progress has been made in optical imaging and detection to provide necessary biological information for disease diagnosis and in-depth understanding of biological ***,recent advances integrating laser technology with biomarkers have enabled novel diagnostic and treatment approaches for different types of diseases.

关键词： optical integrating diagnosis

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Luminescent probes for luminescence lifetime sensing and imaging in live cells:a narrative review

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Journal of Bio-X Research 2020年第4期3卷 174-182页

作者： Kaixuan Nie Xiao Peng Wei Yan Jun Song Junle Qu Center for Biomedical Optics and Photonics&College of Physics and Optoelectronic Engineering Key Laboratory of Optoelectronic Devices and SystemsShenzhen UniversityShenzhenGuangdong ProvinceChina

Luminescence(mostly fluorescence and phosphorescence)probes are a powerful tool widely used in the life sciences *** can be used,for example,in the quantitative analysis of physiological parameters,visualization of different cell organelles,and measurement of drug *** luminescence intensity and lifetime of these probes are among the main signals recorded and evaluated in these *** reviews have discussed optical probes from the perspective of their luminescence ***,the luminescence lifetime,which depends on the molecular microenvironment but not the probe concentration,is another promising metric for biological sensing and imaging *** this review,we aim to introduce the basic strategies of FLIM probe *** also present applications of these probes,including sensing of intracellular pH,cation/anion concentrations,oxygen levels,biomolecule contents,and physiological parameters,as well as live-cell imaging based on luminescence *** based on FLIM imaging of cells or tissues with endogenous organic molecules are not included in this mini *** the rapid development of microscopy technology for fluorescence lifetime imaging,fluorescence lifetime-based probes have shown great potential in a variety of biological applications.

关键词： biological application fluorescence lifetime imaging microscopy luminescence lifetime luminescence probe sensing and imaging

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Efficient coupling of topological photonic crystal waveguides based on transverse spin matching mechanism

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Nature Communications 2025年第1期16卷 1-7页

作者： Shi, Bojian Jia, Qi Li, Xiaoxin Zhang, Yanxia Li, Hang Gao, Yanyu Gao, Wenya Li, Xiaoyu Tang, Donghua Zhu, Tongtong Gao, Shan Yang, Jing Feng, Rui Sun, Fangkui Guan, Chunying Cao, Yongyin Qiu, Cheng-Wei Ding, Weiqiang Institute of Advanced Photonics School of Physics Harbin Institute of Technology Harbin China Department of Physics School of Science Northeast Forestry University Harbin China School of Physics Dalian University of Technology Dalian China College of Physics and Optoelectronic Engineering Harbin Engineering University Harbin China Department of Electrical and Computer Engineering National University of Singapore Singapore Singapore Collaborative Innovation Center of Extreme Optics Shanxi University Shanxi Taiyuan China

Topological photonic structures have great potential to revolutionize on-chip optical integration due to the merit of robust propagation. However, an equally important issue, i.e., the efficient input and output coupling of topological structure with other elements, has not been systematically addressed. Here, we reveal that the coupling of topological photonic structures does not follow the mode matching principle widely used before, but follows the principle of transverse spin matching (TSM). According to this mechanism, efficient coupling between a topological waveguide (TWG) and a strip waveguide (SWG) is designed theoretically and demonstrated experimentally. Theoretical and experimental transmission efficiencies of 96.3% and 94.2% are respectively obtained, which are much larger than those obtained before. The TSM enabled efficient topological structure coupling guarantees a high overall energy efficiency, and paves the way for compact topological photonic chips. © The Author(s) 2025.

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