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A Through-Intact-Skull(TIS)chronic window technique for cortical structure and function observation in mice

eLight 2022年第1期2卷 185-202页

作者： Dongyu Li Zhengwu Hu Hequn Zhang Qihang Yang Liang Zhu Yin Liu Tingting Yu Jingtan Zhu Jiamin Wu Jing He Peng Fei Wang Xi Jun Qian Dan Zhu Britton Chance Center for Biomedical Photonics‑MoE Key Laboratory for Biomedical Photonics Wuhan National Laboratory for Optoelectronics‑Advanced Biomedical Imaging FacilityHuazhong University of Science and TechnologyWuhan 430074HubeiChina Optics Valley Laboratory Hubei 430074China State Key Laboratory of Modern Optical Instrumentations Centre for Optical and Electromagnetic ResearchCollege of Optical Science and EngineeringInternational Research Center for Advanced PhotonicsZhejiang UniversityHangzhou 310058China Interdisciplinary Institute of Neuroscience and Technology Department of AnesthesiologyThe Second Affiliated HospitalSchool of MedicineZhejiang UniversityKaixuan Road 258thHangzhou 310020China Department of Automation Tsinghua UniversityBeijing 100084China School of Optical Electronic Information‑Wuhan National Laboratory for Optoelectronics‑Advanced Biomedical Imaging Facility Huazhong University of Science and TechnologyWuhan 430074HubeiChina

Modern optical imaging techniques provide powerful tools for observing cortical structure and functions at high *** skull windows have been established for different applications of cortical imaging,and each has its advantages and *** critical of the limitations,none of the current skull windows is suitable for observing the responses to some acute craniocerebral injuries on a large scale and at high ***,we developed a“Through-Intact-Skull(TIS)window”that enables the observation of an immune response on a bilateral cortical scale and at single-cell resolution after traumatic brain injury without affecting the pathological environment of the *** TIS window also has the advantages of craniotomy-freeness,centimeter-field of view,synaptic resolution,large imaging depth,long-term observation capability,and suitability for awake ***,the TIS window is a promising new approach for intravital cortical microscopy in basic research in neuroscience.

关键词： Skull optical clearing window Long-term neurovascular imaging Traumatic brain injury Awake animal

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ProxiMO: Proximal Multi-operator Networks for Quantitative Susceptibility Mapping 7th

ProxiMO: Proximal Multi-operator Networks for Quantitative ...

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7th International Workshop on Machine Learning in Clinical Neuroimaging, MLCN 2024, Held in Conjunction with 27th International Conference on Medical Image Computing and Computer-Assisted Intervention, MICCAI 2024

作者： Orenstein, Shmuel Fang, Zhenghan Shin, Hyeong-Geol van Zijl, Peter Li, Xu Sulam, Jeremias Department of Biomedical Engineering Johns Hopkins University BaltimoreMD21218 United States F.M. Kirby Research Center for Functional Brain Imaging Kennedy Krieger Institute BaltimoreMD21205 United States Department of Radiology and Radiological Sciences Johns Hopkins University BaltimoreMD21205 United States Johns Hopkins Kavli Neuroscience Discovery Institute BaltimoreMD21218 United States

ISBN: (纸本)9783031787607

Quantitative Susceptibility Mapping (QSM) is a technique that derives tissue magnetic susceptibility distributions from phase measurements obtained through Magnetic Resonance (MR) imaging. This involves solving an ill-posed dipole inversion problem, however, and thus time-consuming and cumbersome data acquisition from several distinct head orientations becomes necessary to obtain an accurate solution. Most recent (supervised) deep learning methods for single-phase QSM require training data obtained via multiple orientations. In this work, we present an alternative unsupervised learning approach that can efficiently train on single-orientation measurement data alone, named ProxiMO (Proximal Multi-Operator), combining Learned Proximal Convolutional Neural Networks (LP-CNN) with multi-operator imaging (MOI). This integration enables LP-CNN training for QSM on single-phase data without ground truth reconstructions. We further introduce a semi-supervised variant, which further boosts the reconstruction performance, compared to the traditional supervised fashions. Extensive experiments on multi-center datasets illustrate the advantage of unsupervised training and the superiority of the proposed approach for QSM reconstruction. Code is available at https://***/shmuelor/ProxiMO. © The Author(s), under exclusive license to Springer Nature Switzerland AG 2025.

关键词： Mapping

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Functional Connectome-Based Predictive Modeling in Autism

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BIOLOGICAL PSYCHIATRY 2022年第8期92卷 626-642页

作者： Horien, Corey Floris, Dorothea L. Greene, Abigail S. Noble, Stephanie Rolison, Max Tejavibulya, Link O'Connor, David McPartland, James C. Scheinost, Dustin Chawarska, Katarzyna Lake, Evelyn M. R. Constable, R. Todd Interdepartmental Neuroscience Program Yale School of Medicine New Haven Connecticut MD-PhD Program Yale School of Medicine New Haven Connecticut Methods of Plasticity Research Department of Psychology University of Zürich Zurich Switzerland Donders Center for Brain Cognition and Behavior Radboud University Nijmegen Nijmegen The Netherlands Department of Radiology and Biomedical Imaging Yale School of Medicine New Haven Connecticut Yale Child Study Center New Haven Connecticut Department of Biomedical Engineering Yale University New Haven Connecticut Department of Psychology Yale University New Haven Connecticut Department of Statistics and Data Science Yale University New Haven Connecticut Department of Pediatrics Yale School of Medicine New Haven Connecticut Department of Neurosurgery Yale School of Medicine New Haven Connecticut

Autism is a heterogeneous neurodevelopmental condition, and functional magnetic resonance imaging-based studies have helped advance our understanding of its effects on brain network activity. We review how predictive modeling, using measures of functional connectivity and symptoms, has helped reveal key insights into this condition. We discuss how different prediction frameworks can further our understanding of the brain-based features that underlie complex autism symptomatology and consider how predictive models may be used in clinical settings. Throughout, we highlight aspects of study interpretation, such as data decay and sampling biases, that require consideration within the context of this condition. We close by suggesting exciting future directions for predictive modeling in autism.

关键词： Clinical translation Development Fingerprinting Individual differences Machine learning Resting-state fMRI

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Wearable EEG electronics for a Brain–AI Closed-Loop System to enhance autonomous machine decision-making

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npj Flexible Electronics 2022年第1期6卷 296-307页

作者： Joo Hwan Shin Junmo Kwon Jong Uk Kim Hyewon Ryu Jehyung Ok SJoon Kwon Hyunjin Park Tae-il Kim School of Chemical Engineering Sungkyunkwan University(SKKU)Suwon 16419Republic of Korea Department of Electrical and Computer Engineering Sungkyunkwan University(SKKU)Suwon 16419Korea SKKU Institute of Energy Science and Technology(SIEST) Sungkyunkwan University(SKKU)Suwon 16419Korea Center for Neuroscience Imaging Research Institute for Basic ScienceSuwon 16419Korea Biomedical Institue for Convergence(BICS)SKKU Suwon 16419Korea

Human nonverbal communication tools are very ambiguous and difficult to transfer to machines or artificial intelligence(AI).If the AI understands the mental state behind a user’s decision,it can learn more appropriate decisions even in unclear *** introduce the Brain-AI Closed-Loop System(BACLoS),a wireless interaction platform that enables human brain wave analysis and transfers results to AI to verify and enhance AI *** developed a wireless earbud-like electroencephalography(EEG)measurement device,combined with tattoo-like electrodes and connectors,which enables continuous recording of high-quality EEG signals,especially the error-related potential(ErrP).The sensor measures the ErrP signals,which reflects the human cognitive consequences of an unpredicted machine *** AI corrects or reinforces decisions depending on the presence or absence of the ErrP signals,which is determined by deep learning classification of the received EEG *** demonstrate the BACLoS for AIbased machines,including autonomous driving vehicles,maze solvers,and assistant interfaces.

关键词： autonomous EEG enable

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Modifying BiVO4 as a photocatalyst for water oxidation using constant-duration alkaline-etched post treatments

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New Journal of Chemistry 2023年第2期48卷 569-577页

作者： Hsu, Chuan-Chih Chuang, Kai-Jie Huang, Hsiao-Wen Pan, Bo-Jin Chen, Hung-Ming Husain, Sadang Yougbaré, Sibidou Hsiao, Yu-Cheng Wu, Yung-Fu Lin, Lu-Yin Division of Cardiovascular Surgery Department of Surgery School of Medicine College of Medicine Taipei Medical University Taiwan Division of Cardiovascular Surgery Department of Surgery Taipei Medical University Hospital Taiwan Department of Chemical Engineering and Biotechnology National Taipei University of Technology Taipei Taiwan Department of Chemical Engineering Ming Chi University of Technology New Taipei City24301 Taiwan Gingen Technology Co. Ltd. Rm. 7 10F. No. 189 Sec. 2 Keelung Rd. Xinyi Dist. Taipei11054 Taiwan Department of Physics Faculty of Mathematics and Natural Science Lambung Mangkurat University Banjarmasin70124 Indonesia /Nanoro Ouagadougou03 Burkina Faso International PhD Program in Biomedical Engineering College of Biomedical Engineering Taipei Medical University Taipei11031 Taiwan Stanford Byers Center for Biodesign Stanford University CA94305-5428 United States Graduate Institute of Biomedical Optomechatronics College of Biomedical Engineering Taipei Medical University Taipei11031 Taiwan Cell Physiology and Molecular Image Research Center Wan Fang Hospital Taipei Medical University Taipei11696 Taiwan

BiVO4 with suitable band edges and a small band gap is considered an efficient photocatalyst for the oxygen evolution reaction (OER), but short charge-transfer lengths limit its photocatalytic ability. Increasing active sites can essentially enhance oxidation reactions and photocatalytic ability. The alkaline etching technique can refine the surface properties of BiVO4 by introducing oxygen vacancies and possibly establishing a heterojunction, but suitable alkaline etching methods are rarely discussed. In this study, three alkaline etching methods, including hydrothermal, soaking, and electrodeposition processes, are first applied for a constant duration to tailor the surface properties of BiVO4 as a photocatalyst for the OER. Alkaline-etched BiVO4 prepared using hydrothermal and soaking processes shows better photocatalytic abilities due to the generation of additional oxygen vacancies, while poor photocatalytic ability is found for that fabricated using electrodeposition due to the formation of less photocatalytic VO2. The highest photocurrent density of 2.38 mA cm−2 is obtained at 1.23 V versus a reversible hydrogen electrode in an electrolyte without a hole scavenger for the BiVO4 etched in the hydrothermal process, while the untreated BiVO4 presents a photocurrent density of 0.58 mA cm−2. A photocurrent retention of 92.3% is achieved after 5000 seconds of continuous illumination. The study supplies blueprints for establishing effective alkaline etching methods for enhancing the photocatalytic ability of BiVO4, with the exception of time costs. © 2024 The Royal Society of Chemistry.

关键词： Charge transfer

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Enhancing Scene Simplification and Optimization for Retinal Prosthesis Platform

Enhancing Scene Simplification and Optimization for Retinal ...

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Novel Intelligent and Leading Emerging Sciences Conference (NILES)

作者： Heidi Ahmed Holiel Sahar Ali Fawzi Walid Al-Atabany Center for Informatics Science Medical Imaging and Image Processing Research Group Nile University Sheikh Zayed City Egypt Systems and Biomedical Engineering Department Cairo University Giza Egypt Biomedical Engineering Department Helwan University Helwan Egypt

Retinal prostheses are designed to aid individuals with retinal degenerative conditions such as Retinitis Pigmentosa (RP) and Age-related Macular Degeneration (AMD). These prostheses seek to restore vision and improve the perceived scene by stimulating degenerated retinal cells using retinal stimulating electrodes. While these electrodes allow more efficient interaction with the surroundings, they offer limited *** paper presents an innovative approach to revolutionize the visual perception of retinal prosthesis users. The key idea behind the proposed approach is to fuse information extracted from advanced Meanshift and saliency detection algorithms. This proposed approach aims to improve the perceived scene for users, leading to enhanced visual perception and increased efficiency of retinal prostheses.

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The role of input imaging combination and ADC threshold on segmentation of acute ischemic stroke lesion using U-Net

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European Radiology 2023年第9期33卷 6157-6167页

作者： Li, Ya-Hui Lin, Shao-Chieh Chung, Hsiao-Wen Chang, Chia-Ching Peng, Hsu-Hsia Huang, Teng-Yi Shen, Wu-Chung Tsai, Chon-Haw Lo, Yu-Chien Lee, Tung-Yang Juan, Cheng-Hsuan Juan, Cheng-En Chang, Hing-Chiu Liu, Yi-Jui Juan, Chun-Jung Graduate Institute of Biomedical Electronics and Bioinformatics National Taiwan University Taipei Taiwan Department of Medical Imaging China Medical University Hsinchu Hospital No. 199 Sec. 1 Xinglong Rd. Zhubei City Hsinchu County 302 Hsinchu Taiwan Ph.D. Program in Electrical and Communication Engineering Feng Chia University Taichung Taiwan Department of Electrical Engineering National Taiwan University Taipei Taiwan Department of Management Science National Yang Ming Chiao Tung University Hsinchu Taiwan Department of Biomedical Engineering and Environmental Sciences National Tsing Hua University Hsinchu Taiwan Department of Electrical Engineering National Taiwan University of Science and Technology Taipei Taiwan Department of Radiology School of Medicine College of Medicine China Medical University Taichung Taiwan Department of Medical Imaging Medical University Hospital Taichung Taiwan Department of Neurology China Medical University Hospital Taichung Taiwan Cheng Ching Hospital Taichung Taiwan Master’s Program of Biomedical Informatics and Biomedical Engineering Feng Chia University Taichung Taiwan Department of Automatic Control Engineering Feng Chia University No. 100 Wenhwa Rd. Seatwen Taichung 40724 Taiwan Department of Biomedical Engineering The Chinese University of Hong Kong ERB1112 11/F William M.W. Mong Engineering Building N.T Shatin Hong Kong Multi-Scale Medical Robotics Center The Chinese University of Hong Kong N.T Shatin Hong Kong Department of Biomedical Engineering National Defense Medical Center Taipei Taiwan Department of Computer Science and Information Engineering National Taiwan University Taipei Taiwan

Background: To evaluate the effect of the weighting of input imaging combo and ADC threshold on the performance of the U-Net and to find an optimized input imaging combo and ADC threshold in segmenting acute ischemic stroke (AIS) lesion. Methods: This study retrospectively enrolled a total of 212 patients having AIS. Four combos, including ADC-ADC-ADC (AAA), DWI-ADC-ADC (DAA), DWI-DWI-ADC (DDA), and DWI-DWI-DWI (DDD), were used as input images, respectively. Three ADC thresholds including 0.6, 0.8 and 1.8 × 10^–3 mm²/s were applied. Dice similarity coefficient (DSC) was used to evaluate the segmentation performance of U-Nets. Nonparametric Kruskal–Wallis test with Tukey–Kramer post-hoc tests were used for comparison. A p <.05 was considered statistically significant. Results: The DSC significantly varied among different combos of images and different ADC thresholds. Hybrid U-Nets outperformed uniform U-Nets at ADC thresholds of 0.6 × 10^–3 mm²/s and 0.8 × 10^–3 mm²/s (p <.001). The U-Net with imaging combo of DDD had segmentation performance similar to hybrid U-Nets at an ADC threshold of 1.8 × 10^–3 mm²/s (p =.062 to 1). The U-Net using the imaging combo of DAA at the ADC threshold of 0.6 × 10^–3 mm²/s achieved the highest DSC in the segmentation of AIS lesion. Conclusions: The segmentation performance of U-Net for AIS varies among the input imaging combos and ADC thresholds. The U-Net is optimized by choosing the imaging combo of DAA at an ADC threshold of 0.6 × 10^–3 mm²/s in segmentating AIS lesion with highest DSC. Key Points: • Segmentation performance of U-Net for AIS differs among input imaging combos. • Segmentation performance of U-Net for AIS differs among ADC thresholds. • U-Net is optimized using DAA with ADC = 0.6 × 10^–3mm²/s. © 2023, The Author(s), under exclusive licence to European Society of Radiology.

关键词： Deep Learning Diffusion Magnetic Resonance imaging Ischemic Stroke Neural Networks, Computer Retrospective Study

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Advocating for the implementation of SonoVue microbubbles as pressure sensors: a call to action for clinical noninvasive pressure estimation

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Hepatobiliary Surgery and Nutrition 2024年第4期13卷 690-695页

作者： Ao Wen Lingxiao Yang Tao Lv Huayu Yang Fei Li Yilei Mao Gang Xu Jia-Yin Yang Liver Transplant Center Organ Transplant CenterWest China Hospital of Sichuan UniversityChengduChina Laboratory of Liver Transplantation Key Laboratory of Transplant Engineering and ImmunologyNational Health Commission(NHC)West China Hospital of Sichuan UniversityChengduChina West China Hospital West China School of MedicineSichuan UniversityChengduChina Department of Liver Surgery Peking Union Medical College(PUMC)HospitalPUMC and Chinese Academy of Medical SciencesBeijingChina Paul C.Lauterbur Research Center for Biomedical Imaging Shenzhen Institutes of Advanced TechnologyChinese Academy of SciencesShenzhenChina

Pressure measurement within the body is of pivotal significance in the diagnosis of vascular and organ-related diseases associated with hydrostatic *** present,the most commonly used clinical method is to insert a catheter along with a pressure sensor and then guide it to the area of interest through vessels such as central venous pressure(CVP).However,the presence of sensors within the vessel of interest will inevitably cause alterations to the circulation and thus affect blood ***,the use of invasive methods does not allow monitoring of every area inside the body.

关键词： SonoVue microbubbles pressure estimation noninvasive

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Injectable citrate-based polyurethane-urea as a tug-of-war-inspired bioactive self-expansive and planar-fixing screw augmented bone-tendon healing

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Bioactive Materials 2024年第11期41卷 108-126页

作者： Meihan Tao Zhou Fang Yuting Zhu Yan Ju Zhiguo Hou Meimei Fu Zhihui Lu Daozhang Cai Jian Yang Jinshan Guo Department of Orthopedic Surgery Guangdong Provincial Key Laboratory of Bone and Joint Degeneration DiseasesThe Third Affiliated Hospital of Southern Medical UniversityGuangzhou510630PR China Department of Histology and Embryology NMPA Key Laboratory for Safety Evaluation of CosmeticsSchool of Basic Medical SciencesSouthern Medical UniversityGuangzhou510515PR China Regenerative Medicine and Tissue Repair Material Research Center Huangpu Institute of MaterialsGuangzhou New Materials Science CenterChangchun Institute of Applied ChemistryChinese Academy of Sciences88 Yonglong Avenue of Xinlong TownGuangzhou511363PR China Research Center for Industries of the Future Biomedical Engineering ProgramSchool of EngineeringWestlake UniversityHangzhouZhejiang310030PR China CAS Key Laboratory of High-Performance Synthetic Rubber and its Composite Materials Changchun Institute of Applied ChemistryChinese Academy of Sciences5625 Renmin StreetChangchun130022PR China

Inspired by tug-of-war,a game-changing bone-tendon fixation paradigm was ***,injectable citrate-based bioactive self-expansive and planar-fixing screw(iCSP-Scr)consisting of reactive isocyanate(NCO)terminalized citrate-based polyurethane,proanthocyanidin modified hydroxyapatite(HAp)and water(with/without porogen)was developed and administrated in the bone-tendon *** of the“point to point”tendon fixation by traditional interface screws,along with the moisture-induced crosslinking and expansion of iCSP-Scr within the confined space of the irregularly shaped bone-tendon gap,the tendon graft was evenly squeezed into the bone tunnel in a“surface to surface”manner to realize strong and stable bone-tendon fixation via physical expansion,mechanical interlocking and chemical bonding(between–NCO and the–NH2,–SH groups on bone matrix).The optimized iCSP-Scr exhibited rapid crosslinking,moderate expansion rate,high porosity after crosslinking,as well as tunable elasticity and *** iCSP-Scr possessed favorable biodegradability,biocompatibility,and osteoinductivity derived from citrate,PC and HAp,it was able to promote osteogenesis and new bone growth inward of bone tunnel thus further enhanced the bone/iCSP-Scr mechanical interlock,ultimately leading to stronger tendon fixation(pull-out force 106.15±23.15 N)comparing to titanium screws(93.76±17.89 N)after 14 weeks’ACL reconstruction in a rabbit *** iCSP-Scr not only can be used as a self-expansive screw facilitating bone-tendon healing,but also can be expanded into other osteogenic application scenarios.

关键词： Polyurethane Injectable Self-expansive Planar-fixing Citrate

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Spatiotemporal multi-omics:exploring molecular landscapes in aging and regenerative medicine

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Military Medical research 2025年第4期12卷 528-566页

作者： Liu-Xi Chu Wen-Jia Wang Xin-Pei Gu Ping Wu Chen Gao Quan Zhang Jia Wu Da-Wei Jiang Jun-Qing Huang Xin-Wang Ying Jia-Men Shen Yi Jiang Li-Hua Luo Jun-Peng Xu Yi-Bo Ying Hao-Man Chen Ao Fang Zun-Yong Feng Shu-Hong An Xiao-Kun Li Zhou-Guang Wang Affiliated Cixi Hospital Wenzhou Medical UniversityNingbo 315300ZhejiangChina Oujiang Laboratory(Zhejiang Lab for Regenerative Medicine Vision and Brain Health)School of Pharmaceutical ScienceWenzhou Medical UniversityWenzhou 325035ZhejiangChina National Key Laboratory of Macromolecular Drug Development and Manufacturing School of Pharmaceutical ScienceWenzhou Medical UniversityWenzhou 325035ZhejiangChina State Key Laboratory of Bioelectronics School of Biological Science&Medical EngineeringSoutheast UniversityNanjing 210096China School of Pharmaceutical Sciences Guangdong Provincial Key Laboratory of New Drug ScreeningSouthern Medical UniversityGuangzhou 510515China Department of Human Anatomy Shandong First Medical University&Shandong Academy of Medical SciencesTaian 271000ShandongChina Integrative Muscle Biology Laboratory Division of Regenerative and Rehabilitative SciencesUniversity of Tennessee Health Science CenterMemphisTN 38163United States Key Laboratory for Laboratory Medicine Ministry of EducationZhejiang Provincial Key Laboratory of Medical GeneticsSchool of Laboratory Medicine and Life ScienceWenzhou Medical UniversityWenzhou 325035ZhejiangChina Key Laboratory of Imaging Diagnosis and Minimally Invasive Intervention Research Institute of Imaging Diagnosis and Minimally Invasive Intervention Researchthe Fifth Affiliated Hospital of Wenzhou Medical UniversityLishui Hospital of Zhejiang UniversityLishui 323000ZhejiangChina School and Hospital of Stomatology Wenzhou Medical UniversityWenzhou 324025ZhejiangChina Departments of Diagnostic Radiology SurgeryChemical and Biomolecular Engineeringand Biomedical EngineeringYong Loo Lin School of Medicine and College of Design and EngineeringNational University of SingaporeSingapore 119074Singapore Clinical Imaging Research Centre Centre for Translational MedicineYong Loo Lin School of MedicineNational University of SingaporeSingapore 117599Singapore Nanomedicine Translational Research Program NUS Center for Nanom

Aging and regeneration represent complex biological phenomena that have long captivated the scientific *** fully comprehend these processes,it is essential to investigate molecular dynamics through a lens that encompasses both spatial and temporal *** omics methodologies,such as genomics and transcriptomics,have been instrumental in identifying critical molecular facets of aging and ***,these methods are somewhat limited,constrained by their spatial resolution and their lack of capacity to dynamically represent tissue *** advent of emerging spatiotemporal multi-omics approaches,encompassing transcriptomics,proteomics,metabolomics,and epigenomics,furnishes comprehensive insights into these intricate molecular *** sophisticated techniques facilitate accurate delineation of molecular patterns across an array of cells,tissues,and organs,thereby offering an in-depth understanding of the fundamental mechanisms at *** review meticulously examines the significance of spatiotemporal multi-omics in the realms of aging and regeneration *** underscores how these methodologies augment our comprehension of molecular dynamics,cellular interactions,and signaling ***,the review delineates the foundational principles underpinning these methods,followed by an evaluation of their recent applications within the *** review ultimately concludes by addressing the prevailing challenges and projecting future advancements in the ***,spatiotemporal multi-omics are instrumental in deciphering the complexities inherent in aging and regeneration,thus charting a course toward potential therapeutic innovations.

关键词： Spatiotemporal multi-omics Aging and regeneration Cellular interactions Innovative therapeutic strategies

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