Recent work has shown that the vaginal microbiome exerts a strong impact on women's gynecological ***,collection of vaginal specimens is invasive and requires previous clinical training or the involvement of a tra...
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Recent work has shown that the vaginal microbiome exerts a strong impact on women's gynecological ***,collection of vaginal specimens is invasive and requires previous clinical training or the involvement of a trained *** contrast,urine sample collection is routine and noninvasive and does not require involvement of a *** sought to compare the vaginal and urogenital microbiomes to assess the utility of voided urine samples as a proxy for the vaginal *** urogenital and vaginal samples were collected from pregnant women and characterized by 16S rRNA taxonomic *** examined diversities and compositions of paired urogenital and vaginal microbiomes using five discrete strategies to explore the similarity between the vaginal and urogenital microbiomes.A strategy comparing the paired urogenital and vaginal microbiomes in which taxa were assigned using the STIRRUPS database and urine-specific taxa were removed showed no significant difference in diversity and composition between the paired urogenital and vaginal ***,the relative abundances of common vaginal taxa were linearly correlated with those in the paired *** similarities suggest that voided urine samples could represent a noninvasive protocol for accurate profiling of the vaginalmicrobiome with likely clinical ***,a machine learning model was established in which the voided urine microbiome was compared favorably to the vaginal microbiome in predicting bacterial vaginosis.
OBJECTIVE:The objective of this study was to assess alterations in vaginal microbiota and immune markers over the first three months following initiation of copper intrauterine device (copper IUD), levonorgestrel (LNG...
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OBJECTIVE:The objective of this study was to assess alterations in vaginal microbiota and immune markers over the first three months following initiation of copper intrauterine device (copper IUD), levonorgestrel (LNG) implant, and intramuscular depot medroxyprogestone acetate (DMPA-IM).
STUDY DESIGN:We included 162 participants from the Evidence for Contraceptive Options and HIV Outcomes (ECHO) trial, which enrolled healthy, HIV-negative women seeking contraception and randomized them to a copper IUD, LNG implant, or DMPA-IM. microbiome and immune profiles in vaginal swab samples collected at enrollment, 1 month and 3 months were analyzed. We categorized microbiome profiles as 'optimal', 'intermediate', or 'non-optimal' based on established criteria [1]. We compared microbiome and immune markers across contraceptive groups and evaluated changes to 1 and 3 months.
RESULTS:Copper IUD users had a more diverse vaginal microbiome and generally increased inflammatory cytokines and antimicrobial peptides over the 3-month follow-up, compared to LNG-implant and DMPA-IM users [2]. LNG-implant users had less complex vaginal microbiomes with reduced inflammation, while DMPA-IM showed little change in either microbiome composition or inflammatory markers. Copper IUD users exhibited lower microbiome stability and a higher likelihood of transitioning to less optimal profiles. In contrast, LNG-implant users showed greater stability and a higher probability of transition to optimal microbiome and immune marker profiles.
CONCLUSIONS:Contraceptive methods affect the vaginal microbiome differently. Copper IUD use may lead to less favorable profiles and increased levels of some immune markers, indicating potential adverse health effects. Conversely, LNG-implant usage promotes a more favorable microbiome and immune marker balance.
IMPLICATIONS:Our findings suggest that copper IUDs are associated with decreased prevalence of Lactobacillus-dominated microbiomes, higher transition rates to
The vaginal microbiome likely influences host signaling compounds within the reproductive tract,including pro-inflammatory signals,which may play an important role during *** lactobacilli are associated with positive ...
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The vaginal microbiome likely influences host signaling compounds within the reproductive tract,including pro-inflammatory signals,which may play an important role during *** lactobacilli are associated with positive pregnancy outcome,whereas bacterial vaginosis,a dysbiosis of the vaginal microbiome,is associated with an increased risk of adverse pregnancy outcomes including preterm *** the host response could be predicted based on the taxonomic composition of the vaginal microbiome,particularly early in pregnancy,then those predictions could potentially be used to personalize intervention methods to reduce preterm birth and other adverse *** this proof of principle study,we apply multivariate strategies to analyze 16S rRNA-based taxonomic surveys in conjunction with targeted immuno-proteomic and lipidomic data from vaginal samples from 58 women enrolled in the Multi-Omic microbiome Study-Pregnancy Initiative during early *** between the vaginal microbiome and the vaginal lipidome have not been previously *** from this study reveal significant multiple pairwise associations between microbial taxa,specific eicosanoids and sphingomyelins,and *** the biologic significance of these associations is not yet known,these results support the utility of such multi-omic approaches as a means to predict the impact of the microbiome on the host.
Litter decomposition plays a key role in nutrient cycling across ecosystems. The presence of Epichloë endophytes in grass plants affects litter decomposition. The overall effects of leaf litter containing Epichlo...
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Litter decomposition plays a key role in nutrient cycling across ecosystems. The presence of Epichloë endophytes in grass plants affects litter decomposition. The overall effects of leaf litter containing Epichloë endophytes on the chemical and biological properties in non-rhizosphere soils have not previously been examined. The current study is aimed to understand the effect of direct incorporation of leaf litter from Epichloë endophyte-infected perennial ryegrass ( Lolium perenne ) on soil chemical characteristics, and fungal and bacterial biodiversity. Firstly, fresh leaf litter of the endophyte Epichloë festucae var. lolii -infected (E+) and endophyte-free (E−) L. perenne were incorporated into the soil, and then samples were collected from E+ and E− litter incorporated soil at T 0 , T 1 , T 2 and T 3 (0, 120, 240 and 360 days) for chemical and sequencing analyses. The Epichloë -containing leaf litter incorporation changed the soil microbial environment by enhancing the soil organic carbon (SOC), total nitrogen (TN), total phosphorus, NH 4 + –N and NO 3 − –N contents, and acidifying soil pH. The endophyte had stronger effects on soil bacteria than soil fungi when the litter was determined with and without the endophyte. Litter containing the Epichloë endophyte significantly increased the absolute abundance of the 16S rRNA gene and relative abundances of the Actinobacteria, Nitrospirae and Gemmatimonadetes, while significantly decreasing the relative abundances of the Planctomycetes and Rokubacteria and the diversity in soil bacteria community. There was no significant effect of endophyte-containing leaf litter incorporation on the absolute abundance and diversity of the soil fungal community except the increment in the relative abundance of Rozellomycota. Soil pH and the SOC-to-TN ratio (C:N) were the main factors influencing the soil bacterial community, and the presence of E. festucae var. lolii was directly and positively related to the soil bacterial diversi
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