检索结果-内蒙古大学图书馆

Validation of methods for enriching and detecting SARS-CoV-2 RNA in wastewater

environmental Chemistry Letters 2025年 1-6页

作者： Li, Ruixue Wang, Nan Guo, Jingjing Xue, Boyuan Liu, Chun Guo, Yong Zhou, Xiaohong School of Environmental Science and Engineering Hebei University of Science and Technology Shijiazhuang050000 China Center for Sensor Technology of Environment and Health School of Environment Tsinghua University Beijing100084 China Department of Biomedical Engineering Tsinghua University Beijing100084 China Tianjin Ecological and Environmental Monitoring Center Tianjin300191 China

Monitoring virus concentrations in wastewater is crucial for tracking community viral spread, yet reliable virus enrichment and detection methods are lacking. We compared four preconcentration-detection methods for tracing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in wastewater: ultrafiltration and covalent affinity resin separation, paired with either reverse transcription-quantitative polymerase chain reaction (PCR) or reverse transcription-digital PCR. Tests were conducted on raw sewage from a campus dormitory septic tank in May 2023. Results show that the concentrations of coronavirus RNA in wastewater enriched by ultrafiltration were higher than those enriched by covalent affinity resin separation, regardless of the detection methods used. Digital PCR exhibited detection rates of 45.4% using covalent affinity resin separation, and 77.3% using ultrafiltration. These detection rates were 36.4% and 72.7% higher than those using quantitative PCR. A correlation coefficient of 0.70 between concentrations measured by quantitative and digital PCR was only observed for samples enriched by covalent affinity resin separation. © The Author(s), under exclusive licence to Springer Nature Switzerland AG 2025.

关键词： Ultrafiltration

来源：评论

学校读者我要写书评

暂无评论

Nanoplastics drive toxicity under co-exposure with perfluorooctanesulfonic acid in human intestinal cells

引用

environmental Chemistry Letters 2025年 1-9页

作者： Alijagic, Andi Särndahl, Eva Kotlyar, Oleksandr Karlsson, Patrik Duberg, Daniel Scherbak, Nikolai Pinsino, Annalisa Engwall, Magnus Hyötyläinen, Tuulia Man-Technology-Environment Research Center Örebro University Örebro701 82 Sweden Inflammatory Response and Infection Susceptibility Centre Örebro University Örebro701 82 Sweden School of Medical Sciences Faculty of Medicine and Health Örebro University ÖrebroSE-701 82 Sweden Centre for Applied Autonomous Sensor Systems Robot Navigation & Perception Lab Örebro University Örebro701 82 Sweden Department of Mechanical Engineering Örebro University Örebro701 82 Sweden Institute of Translational Pharmacology National Research Council Via Ugo La Malfa 153 Palermo90146 Italy

Per- and polyfluoroalkyl substances and nanoplastics frequently co-occur in environmental matrices, yet the effects of co-exposure on cellular responses upon ingestion are poorly understood. Here, we exposed human intestinal Caco-2 cells to perfluorooctanesulfonic acid, nanoplastics, and their combination. Cell painting-based phenomics was used to map phenotypic alterations across subcellular structures, and untargeted metabolomics using ultra-high-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry was employed to assess metabolic changes. Results show that perfluorooctanesulfonic acid predominantly affected the actin cytoskeleton, Golgi apparatus, and plasma membrane, while nanoplastics primarily targeted mitochondria. Combined exposure disrupted the endoplasmic reticulum, RNA, and mitochondria. Perfluorooctanesulfonic acid reduced levels of carnitines, free fatty acids, nucleotides, and sugars, whereas nanoplastics inhibited ceramides, triglycerides, sphingomyelins, and additional free fatty acids. Combined exposure produced a metabolic profile resembling that of nanoplastics, with specific differences attributed to perfluorooctanesulfonic acid. Overall, nanoplastics appear as the main drivers of the co-exposure effects. © The Author(s) 2025.

关键词： Nucleotides

来源：评论

学校读者我要写书评

暂无评论

Optimization of transesterification of sewage sludge for biodiesel production using monosaccharide-, disaccharide-, and polysaccharide-based sulfonic acid solid catalysts

引用

Journal of environmental Chemical Engineering 2025年第3期13卷

作者： Yiyu Wang Renhua Chen Fenfen Zhu Huan Wang Yuqun Qiu Boyuan Xue Bing Zhao Tiantian Zhou School of Chemistry and Life Resources Renmin University of China Beijing 100872 China State Key Joint Laboratory of ESPC Center for Sensor Technology of Environment and Health School of Environment Tsinghua University Beijing 100084 China

Sewage sludge is rich in lipids and organic matter; hence, it is a potential resource for biodiesel production. However, an efficient, cost-effective, and environment-friendly catalyst is required for the esterification or transesterification of lipids present in sewage sludge into biodiesel. In this study, we prepared monosaccharide-, disaccharide-, polysaccharide-based solid acid catalysts by a new method which is to mix saccharides directly with concentrated sulfuric acid to accomplish the carbonization and sulfonation process simultaneously. We also compared their catalytic efficiencies in terms of biodiesel yield. The results revealed that the monosaccharide-based solid acid catalyst exhibited the best catalytic efficiency, with a 93.7 % lipid transformation rate, which is due to its smaller particle size and larger surface area. Moreover, we determined that the optimal conditions for biodiesel production were as follows: 130 ℃ temperature, 4-h reaction time; catalyst-to-lipid mass ratio of 1, and methanol-to-lipid mass ratio of 25. Furthermore, the solid catalysts could be simply recovered by rinsing with minor loss of catalytic activity within five cycles. These findings prove that the monosaccharide-based solid acid catalyst can effectively catalyze the transesterification of the lipids in sewage sludge into biodiesel.

关键词： Saccharides based solid acid catalyst Sewage sludge Biodiesel Direct carbonization and sulfonation

来源：评论

学校读者我要写书评

暂无评论

Label-free colorimetric nanosensor with improved sensitivity for Pb^2+ in water by using a truncated 8-17 DNAzyme

引用

Frontiers of environmental Science & Engineering 2019年第1期13卷 129-134页

作者： Abdul Ghaffar Memon Xiaohong Zhou Yunpeng Xing Ruoyu Wang Lanhua Liu Mohsin Khan Miao He Center for Sensor Tech no logy of Environment and Health State Key Joint Laboratory of ESPC School of Environment Tsinghua University Beijing 100084 China Department of Environmental Engineering NED University of Engineering and Technology Karachi 75270. Pakistan

Water pollution accidents, such as the Flint water crisis in the United States, caused by lead contamination have raised concern on the safety of drinking water distribution systems. Thus, the routine monitoring of lead in water is highly required and demands efficient, sensitive, cost-effective, and reliable lead detection methods. This study reports a label-free colorimetric nanosensor that uses unmodified gold nanoparticles (AuNPs) as indicators to enable rapid and ultra-sensitive detection of lead in environmental water. The 8-17 DNAzyme was truncated in this study to facilitate the detachment of single-stranded DNA fragments after substrate cleavage in the presence of Pb「. The detached fragments were adsorbed over AuNPs and protected against salt concentration-induced aggregation. Accordingly, high Pb^2+ would result in rapid color change from blue to pink. The established sensing principle achieved a sensitive limit of detection of 0.2×10^-9mol/L Pb^2+. with a linear working range of two orders of magnitude from 0.5×10^-9mol/L to 5×10^-9mol/L. The selectivity of the nanosensor was demonstrated by evaluating the interfering metal ions. The developed nanosensor can serve as a substitute for the rapid analysis and monitoring of trace lead levels under the drinking water distribution system and even other environmental water samples.

关键词： Colorimetric nanosensor Truncated 8-17 DNAzyme Pb^2+ detection Unmodified AuNPs

来源：评论

学校读者我要写书评

暂无评论

A cellphone-based colorimetric multi-channel sensor for water environmental monitoring

引用

Frontiers of environmental Science & Engineering 2022年第12期16卷 67-78页

作者： Yunpeng Xing Boyuan Xue Yongshu Lin Xueqi Wu Fang Fang Peishi Qi Jinsong Guo Xiaohong Zhou State Key Laboratory of Urban Water Resource and Environment School of EnvironmentHarbin Institute of TechnologyHarbin150090China State Key Joint Laboratory of ESPC Center for Sensor Technology of Environment and HealthSchool of EnvironmentTsinghua UniversityBeijing100084China Key Laboratory of the Three Gorges Reservoir Region’s Eco-Environments of MOE Chongqing UniversityChongqing400030China

The development of colorimetric analysis technologies for the commercial cellphone platform has attracted great attention in environmental monitoring due to the low cost,high versatility,easy miniaturization,and widespread ownership of *** work demonstrates a cellphone-based colorimetric multi-channel sensor for quantifying multiple environmental contaminants simultaneously with high sensitivity and *** improve the sensitivity of the sensor,a delicate optical path system was created by using a diffraction grating to split six white beams transmitting through the multiple colored samples,which allows the cellphone CMOS camera to capture the diffracted light for image *** proposed sensor is a universal colorimetric detection platform for a variety of environmental contaminants with the colorimetry assay in the range of 400–700 *** introducing the diffraction grating for splitting light,the sensitivity was improved by over six folds compared with a system that directly photographed transmitted *** a successful proof-of-concept,the sensor was used to detect turbidity,orthophosphate,ammonia nitrogen and three heavy metals simultaneously with high sensitivity(turbidity:detection limit of 1.3 NTU,linear range of 5–400 NTU;ammonia nitrogen:0.014 mg/L,0.05–5 mg/L;orthophosphate:0.028 mg/L,0.1–10 mg/L;Cr(VI):0.0069 mg/L,0.01–0.5 mg/L;Fe:0.025 mg/L,0.1–2 mg/L;Zn:0.032 mg/L,0.05–2 mg/L)and reliability(relative standard deviations of six parallel measurements of 0.37%–1.60%and recoveries of 95.5%–106.0%in surface water).The miniature sensor demonstrated in-field sensing ability in environmental monitoring,which can be extended to point-of-care diagnosis and food safety control.

关键词： Colorimetric analysis Multi-channel sensor Cellphone Water quality indexes environmental monitoring

来源：评论

学校读者我要写书评

暂无评论

MODIFIED FLEXIBLE MICRONEEDLE ELECTRODE ARRAY UTILIZING MULTI-LAYERED NANOSTRUCTURE FOR WIDE LINEAR RANGE NONENZYMATIC GLUCOSE MONITORING 25

MODIFIED FLEXIBLE MICRONEEDLE ELECTRODE ARRAY UTILIZING MULT...

引用

25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021

作者： Xia, Kaidong Li, Junshi Huang, Dong Zhou, Xiaohong Li, Zhihong National Key Laboratory of Science and Technology on Micro/Nano Fabrication Institute of Microelectronics Peking University Beijing100871 China State Key Joint Laboratory of ESPC Center for Sensor Technology of Environment and Health School of Environment Tsinghua University Beijing100084 China

ISBN: (纸本)9781733419031

In this work, we proposed a flexible patch based on microneedle electrode array, which is used to nonenzymatically monitor blood glucose with minimal invasiveness. Biocompatible Nafion/Pt black/ZnO nanosheet composites were constructed on the surface of microneedle arrays. Wide range monitoring of human blood glucose in interstitial fluid was achieved through in vitro mimetic experiments with the synergistic effects between selected components with different physical and chemical properties. The fabrication process was inexpensive and effective. The integration of the sensing device was realized with a simplified two-electrode system, which has the clinical application potential for continuous monitoring of human biochemical parameters. © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.

关键词： II-VI semiconductors

来源：评论

学校读者我要写书评

暂无评论

De novo design of covalent bondingpeptides for target protein

引用

hLife 2024年第12期2卷 642-652页

作者： Xiaohong Zhou Qian Zhu Anqi Zheng Boyuan Xue Qihui Wang Lip Ket Chin Jingkun Jiang Miao He Center for Sensor Technology of Environment and Health School of EnvironmentTsinghua UniversityBeijingChina CAS Key Laboratory of Pathogen Microbiology and Immunology Institute of MicrobiologyChinese Academy of SciencesBeijingChina Department of Electrical Engineering City University of Hong KongHong KongChina

To rapidly develop hyper-stable inhibitors that bind specifically and covalently to functional proteins is critical for diagnostics and therapeutics. Taking targeting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants asan example, we report a fast and low-cost de novo design strategy on covalent bonding peptides toward the SARS-CoV2 spike protein receptor-binding domain (RBD), hence blocking its interaction with the human angiotensin-convertingenzyme 2 (hACE2). As a proof-of-concept, peptide scaffolds built by ligating the hotspot residues at the hACE2-OmicronRBD interface were docked against RBD, and then the chemically modified peptides were designed by predicting theirreactivity against RBD using a modified Amber ff14SB force field. Two peptides (15- and 16-mer peptides) were equipped with sulfonyl fluoride warheads bound with the conserved Y449 residue of RBD via the sulfur (VI) fluoride exchange(SuFEx) click chemistry. With permanent bonding and without dissociation, the two peptides blocked Omicron BA.2pseudovirus infection with 50% inhibitory concentration (IC50) values of 1.07 mM and 1.56 mM, respectively. Our designapproach greatly promotes the discovery of hyper-stable inhibitors against SARS-CoV-2 variants and other rapidlyevolving viruses, potentially applicable to combat future viral outbreaks efficiently.

关键词： peptides covalent bonding de novo design severe acute respiratory syndrome coronavirus 2(SARSCoV-2)

来源：评论

学校读者我要写书评

暂无评论

De novo design of covalent bonding peptides for target protein

微生物与宿主健康（英文）

引用

微生物与宿主健康（英文） 2024年第12期2卷 641-652页

To rapidly develop hyper-stable inhibitors that bind specifically and covalently to functional proteins is critical for diag-nostics and *** targeting severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)variants as an example,we report a fast and low-cost de novo design strategy on covalent bonding peptides toward the SARS-CoV-2 spike protein receptor-binding domain(RBD),hence blocking its interaction with the human angiotensin-converting enzyme 2(hACE2).As a proof-of-concept,peptide scaffolds built by ligating the hotspot residues at the hACE2-Omicron RBD interface were docked against RBD,and then the chemically modified peptides were designed by predicting their reactivity against RBD using a modified Amber ff14SB force *** peptides(15-and 16-mer peptides)were equip-ped with sulfonyl fluoride warheads bound with the conserved Y449 residue of RBD via the sulfur(VI)fluoride exchange(SuFEx)click *** permanent bonding and without dissociation,the two peptides blocked Omicron BA.2 pseudovirus infection with 50％inhibitory concentration(IC50)values of 1.07 μM and 1.56 μM,*** design approach greatly promotes the discovery of hyper-stable inhibitors against SARS-CoV-2 variants and other rapidly evolving viruses,potentially applicable to combat future viral outbreaks efficiently.

关键词： peptides covalent bonding de novo design severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)

来源：评论

学校读者我要写书评

暂无评论

OS03-08 Comprehensive chemical and toxicological screening of e-waste plastic chemicals

引用

Toxicology Letters 2024年 399卷 S66-S66页

作者： A. Alijagic F. Södergren Seilitz A. Bredberg A. Hakonen M. Larsson V. Sjöberg O. Kotlyar A. Persson N. Scherbak D. Repsilber A. Kärrman T. Wang E. Särndahl M. Engwall Örebro University Man-Technology-Environment Research Center Örebro Sweden Örebro University Inflammatory Response and Infection Susceptibility Centre Örebro Sweden Örebro University School of Medical Sciences Faculty of Medicine and Health Örebro Sweden Research Institutes of Sweden Gothenburg Sweden Sensor Visions AB Gothenburg Sweden Örebro University Centre for Applied Autonomous Sensor Systems Örebro Sweden Linköping University Department of Physics Chemistry and Biology Linköping Sweden

来源：评论

学校读者我要写书评

暂无评论

Correction: The role of electrochemical biosensors in SARS-CoV-2 detection: a bibliometrics-based analysis and review

引用

RSC advances 2022年第48期12卷 31441页

作者： Shuduan Mao Li Fu Chengliang Yin Xiaozhu Liu Hassan Karimi-Maleh Key Laboratory of Pollution Exposure and Health Intervention of Zhejiang Province Interdisciplinary Research Academy (IRA) Zhejiang Shuren University Hangzhou 310015 China. Key Laboratory of Novel Materials for Sensor of Zhejiang Province College of Materials and Environmental Engineering Hangzhou Dianzi University Hangzhou 310018 China fuli@***. National Engineering Laboratory for Medical Big Data Application Technology Chinese PLA General Hospital Beijing China. Medical Big Data Research Center Medical Innovation Research Division of PLA General Hospital Beijing China. Department of Cardiology The Second Affiliated Hospital of Chongqing Medical University Chongqing 400010 China. School of Resources and Environment University of Electronic Science and Technology of China Xiyuan Ave 611731 Chengdu China. Department of Chemical Engineering Quchan University of Technology Quchan *** Iran. Department of Chemical Sciences University of Johannesburg Doornfontein Campus 2028 Johannesburg 17011 South Africa.

[This corrects the article DOI: 10.1039/D2RA04162F.].

关键词：

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：