检索结果-内蒙古大学图书馆

Ginsenoside Rg5 alleviates radiation-induced acute lung vascular endothelium injury by reducing mitochondrial apoptosis via Sirt1

引用

Journal of Ginseng Research 2025年第3期49卷 260-270页

作者： Li, Churong Zhao, Biao Xiong, Jing Li, Linjie Pang, Dalong Unger, Keith Jung, Mira Lyu, Jiahua Kuang, Hao Liang, Long Li, Tao Chen, Long Bai, Hansong Radiotherapy Center Radiation Oncology Key Laboratory of Sichuan Province Sichuan Clinical Research Center for Cancer Sichuan Cancer Hospital & Institute Sichuan Cancer Center University of Electronic Science and Technology of China Chengdu China Non-Coding RNA and Drug Discovery Key Laboratory of Sichuan Province School of Basic Medical Sciences Chengdu Medical College Chengdu China Department of Integrative Chinese and Western Medicine Sichuan Cancer Hospital & Institute Sichuan Cancer Center Affiliated Cancer Hospital of the University of Electronic Science and Technology of China Chengdu China Department of Immunology School of Basic Medical Sciences Chengdu Medical College Chengdu China Department of Radiation Medicine Lombardi Comprehensive Cancer Center Georgetown University Medical Center Washington DC United States Department of Oncology The Affiliated Hospital of Southwest Medical University Luzhou China School of Medicine University of Electronic Science and Technology of China Chengdu China

Background: Ginsenoside Rg5 possesses potent anti-oxidative, anti-inflammatory, and cytoprotective properties. This study explored the protective effects of ginsenoside Rg5 on radiation-induced pulmonary microvascular endothelial cells (PMECs) injury and the associated molecular mechanisms. Materials and methods: C57BL/6 mice were used for in vivo studies and primary human PMECs (PPMECs) were utilized as in vitro models. Mice with or without ginsenoside Rg5 pretreatment were irradiated by varying doses. Lung tissues were analyzed for histopathological changes and the expression of endothelial markers. In vitro, PPMECs were irradiated with or without ginsenoside Rg5 pretreatment and analyzed for apoptosis, oxidative stress, mitochondrial function, and endothelial barrier integrity. Results: Ginsenoside Rg5 pretreatment attenuated radiation-induced acute lung damage, preserved endothelial cell junction integrity, and maintained endothelial barrier function in vivo. In vitro, ginsenoside Rg5 significantly reduced IR-induced oxidative stress, apoptosis, and mitochondrial dysfunction in PPMECs. Ginsenoside Rg5 suppressed radiation-induced Mfn2 acetylation and proteasomal degradation via Sirt1-mediated deacetylation, thereby preserving mitochondrial dynamics and integrity. The protective effects of ginsenoside Rg5 on the integrity of mitochondrial and endothelial tight junction proteins and barrier function were also Sirt1-dependent. Conclusions: Ginsenoside Rg5 exerts a protective effect against radiation-induced endothelial injury by modulating mitochondrial dynamics and function, as well as maintaining endothelial barrier integrity, in a Sirt1-dependent manner. © 2025

关键词： Endothelial cell injury Ginsenoside Rg5 Mitochondrial dynamics Radiation Sirt1

来源：评论

学校读者我要写书评

暂无评论

Transposon–host arms race: a saga of genome evolution

引用

Trends in Genetics 2025年第5期41卷 369-389页

作者： Iwasaki, Yuka W. Shoji, Keisuke Nakagwa, Shinichi Miyoshi, Tomoichiro Tomari, Yukihide Laboratory for Functional Non-coding Genomics RIKEN Center for Integrative Medical Sciences 1-7-22 Suehiro-cho Tsurumi-ku Kanagawa Yokohama 230-0045 Japan of Bio-Applications and Systems Engineering Tokyo University of Agriculture and Technology Tokyo Koganei-shi 184-8588 Japan Laboratory of RNA Function Institute for Quantitative Biosciences The University of Tokyo 1-1-1 Yayoi Tokyo Bunkyo-ku 113-0032 Japan RNA Biology Laboratory Faculty of Pharmaceutical Sciences Hokkaido University Kita 12-jo Nishi 6-chome Kita-ku Sapporo 060-0812 Japan Laboratory for Retrotransposon Dynamics RIKEN Center for Integrative Medical Sciences 1-7-22 Suehiro-cho Tsurumi-ku Kanagawa Yokohama 230-0045 Japan Department of Computational Biology and Medical Sciences of Frontier Sciences The University of Tokyo Tokyo Bunkyo-ku 113-0032 Japan

Once considered ‘junk DNA,’ transposons or transposable elements (TEs) are now recognized as key drivers of genome evolution, contributing to genetic diversity, gene regulation, and species diversification. However, their ability to move within the genome poses a potential threat to genome integrity, promoting the evolution of robust host defense systems such as Krüppel-associated box (KRAB) domain-containing zinc finger proteins (KRAB-ZFPs), the human silencing hub (HUSH) complex, 4.5SH rnas, and PIWI-interacting rnas (pirnas). This ongoing evolutionary arms race between TEs and host defenses continuously reshapes genome architecture and function. This review outlines various host defense mechanisms and explores the dynamic coevolution of TEs and host defenses in animals, highlighting how the defense mechanisms not only safeguard the host genomes but also drive genetic innovation through the arms race. © 2025 Elsevier Ltd

关键词： evolutionary arms race genetic conflicts genome evolution host defense mechanisms transposable elements (TEs)

来源：评论

学校读者我要写书评

暂无评论

Inhibition of KDM6B prevents osteoarthritis by blocking growth plate-like H3K27me3 loss in bivalent genes

引用

Science China Life Sciences 2025年第5期68卷 1423-1436页

作者： Hao Du Yao Zhang Xi Yu Xuanhe You Diwei Wu Ze Du Yongrui Cai Zhenyu Luo Hanpeng Lu Zhixin Liao Bi-Sen Ding Ya Zhao Yan Wang Ke Xiao Fan Yang Fangji Gan Ning Ning Jiancheng Zeng Peiliang Shi Zongke Zhou Shishu Huang Department of Orthopedic Surgery and Orthopedic Research Institute West China HospitalSichuan University Rehabilitation Medicine Center West China HospitalSichuan University Key Laboratory of Birth Defects and Related Diseases of Women and Children of MOE State Key Laboratory of BiotherapyWest China Second University HospitalSichuan University Institute of Translational Medicine Medical CollegeYangzhou University Jiangsu Key Laboratory of Experimental & Translational Non-coding RNA Research College of Animal Science and Technology Sichuan Agricultural University Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province Sichuan Agricultural University Department of Lower Limb Surgery of Sichuan Province Orthopedic Hospital Central Laboratory of Sichuan Province Orthopedic Hospital Brain Cognition and Brain Disease Institute (BCBDI) Shenzhen Institute of Advanced TechnologyChinese Academy of Sciences (CAS)Shenzhen-Hong Kong Institute of Brain Science-Shenzhen Fundamental Research Institutions School of Mechanical Engineering Sichuan University Gem Pharmatech (Chengdu) Co. Ltd.

Osteoarthritis(OA) is the most prevalent joint disorder occurring with articular cartilage degradation. It includes a switch from an articular to a growth plate chondrocyte phenotype. Here, we investigated the histone modification profiles and found significant H3K27me3 loss in OA, which led to disease-associated gene expression. Surprisingly, these genes were occupied by both H3K27me3 and H3K4me3 in normal chondrocytes, showing a poised bivalent state. Furthermore, we observed the derepression of similar bivalent genes in growth plate chondrocytes. Finally, a KDM6B inhibitor GSK-J4 prevented the H3K27me3 loss and cartilage damage in the rat OA model. Our results reveal an inherited bivalent epigenetic signature on developmental genes that makes articular chondrocytes prone to hypertrophy and contributes to a promising epigenetic therapy for OA.

关键词： osteoarthritis histone modifications H3K27me3 bivalency growth plate

来源：评论

学校读者我要写书评

暂无评论

Improved immunocompatibility of active targeting liposomes by attenuating nucleophilic attack of cyclic RGD peptides on complement 3

引用

Biomaterials 2025年 321卷 123350-123350页

作者： Meng, Nana Lu, Jiasheng Zhou, Jianfen Yang, Shengmin Zhang, Chen Jia, Ruiyi Ding, Yuan Bao, Yanning Wang, Jun Ma, Xiaopei Chen, Ruohan Jiang, Zhixuan Xie, Cao Lu, Linwei Lu, Weiyue Department of Pharmaceutics School of Pharmacy Fudan University & National Key Laboratory of Advanced Drug Formulations for Overcoming Delivery Barriers Shanghai201203 China Department of Integrative Medicine Huashan Hospital Fudan University Shanghai200040 China Institutes of Integrative Medicine Fudan University Shanghai200040 China Shanghai Engineering Technology Research Center for Pharmaceutical Intelligent Equipment and Shanghai Frontiers Science Center for Druggability of Cardiovascular Non-coding RNA Institute for Frontier Medical Technology Shanghai University of Engineering Science Shanghai201620 China

One of the challenges for the clinical translation of active targeting nanomedicines is the adverse interactions between targeting ligands and blood components. Herein, a novel regularity, which reveals the interactions between cyclic RGD (Arg-Gly-Asp) peptide-modified liposomes and complement components in blood, is reported. As the nucleophilicity of arginine guanidine group within the cyclic RGD-like peptide increases, targeting liposomes potentiate complement cascade via the amplification loop of complement 3 (C3), ultimately leading to accelerated blood clearance, increased deposition in the reticuloendothelial system (RES) organs, enhanced immune responses, and potential side effects. By appropriately reducing the nucleophilicity of guanidine group, cyclic R2 peptide is designed for modification of liposomes to target integrin αvβ3. Compared to the widely used targeting molecule c(RGDyK), R2 eliminates the negative effects of C3 opsonization and specific antibody production, significantly improves the in vivo immunocompatibility of targeting liposomes, and demonstrates superior anti-tumor efficacy in mouse models of orthotopic breast cancer and glioma. Thus, the proposed regularity of interactions between guanidine nucleophilicity and C3, along with the successful application of the low complement activation capacity targeting ligand R2, provides new insights for addressing challenges related to complement activation in the clinical translation of active targeting nanomedicines. © 2025 Elsevier Ltd

关键词： Ligands

来源：评论

学校读者我要写书评

暂无评论

Correction: Ribosomal modification protein rimK-like family member A activates betaine-homocysteine S-methyltransferase 1 to ameliorate hepatic steatosis

引用

Signal Transduction and Targeted Therapy 2025年第1期10卷 471-472页

作者： Han Yan Wenjun Liu Rui Xiang Xin Li Song Hou Luzheng Xu Lin Wang Dong Zhao Xingkai Liu Guoqing Wang Yujing Chi Jichun Yang Department of Physiology and Pathophysiology School of Basic Medical Sciences State Key Laboratory of Vascular Homeostasis and Remodeling Center for Non-coding RNA Medicine Peking University Health Science Center Beijing China Department of Endocrinology The Second Affiliated Hospital School of Medicine Zhejiang University Hangzhou China Department of Cardiology Peking University Third Hospital Beijing China Medical and Health Analysis Center Peking University Beijing China Department of Hepatobiliary Surgery Xi-Jing Hospital Fourth Military Medical University Xi’an China Department of Endocrinology Beijing Luhe Hospital Capital Medical University Beijing China Department of Hepatobiliary and Pancreatic Surgery General Surgery Centre First Hospital of Jilin University Changchun China Key Laboratory of Pathobiology Ministry of Education College of Basic Medical Sciences Jilin University Changchun China Department of Central Laboratory and Institute of Clinical Molecular Biology Department of Gastroenterology Peking University People’s Hospital Beijing China

Correction to:Signal Transduction and Targeted Therapy https://***/10.1038/s41392-024-01914-0,published online 08 August 2024 In the process of collating the raw data,the authors noticed one inadvertent mistake in supplementaryfigure 9f after online publication of the ***β-actin in supplementaryfigure 9d was repeatedly inserted asβ-actin(upper)in supplementaryfigure *** correct data and corresponding original image are provided as *** correction does not affect the keyfindings(the existing published results or the discussion).The original article has been corrected.

关键词： hepatic correction repeatedly

来源：评论

学校读者我要写书评

暂无评论

Quality Assessment of Domesticated Animal Genome Assemblies

引用

Bioinformatics and Biology Insights 2015年第Suppl 4期9S4卷 49-58页

作者： Seemann, Stefan E. Anthon, Christian Palasca, Oana Gorodkin, Jan Center for non-coding RNA in Technology and Health Department of Veterinary Clinical and Animal Sciences University of Copenhagen København Denmark

The era of high-throughput sequencing has made it relatively simple to sequence genomes and transcriptomes of individuals from many species. In order to analyze the resulting sequencing data, high-quality reference genome assemblies are required. However, this is still a major challenge, and many domesticated animal genomes still need to be sequenced deeper in order to produce high-quality assemblies. In the meanwhile, ironically, the extent to which rna seq and other next-generation data is produced frequently far exceeds that of the genomic sequence. Furthermore, basic comparative analysis is often affected by the lack of genomic sequence. Herein, we quantify the quality of the genome assemblies of 20 domesticated animals and related species by assessing a range of measurable parameters, and we show that there is a positive correlation between the fraction of mappable reads from rnaseq data and genome assembly quality. We rank the genomes by their assembly quality and discuss the implications for genotype analyses. © 2015, © 2015 SAGE Publications.

关键词： assembly quality domesticated animals genome assembly

来源：评论

学校读者我要写书评

暂无评论

Computational strategies to search structured rnas in genomic sequence(and roads towards further analysis)

Computational strategies to search structured RNAs in genomi...

引用

2011国际基因组学大会

作者： Jan Gorodkin Center for non-coding RNA in Technology and Health IBHVUniversity of CopenhagenDenmark

A key application of early methods for computational rna folding was to aid interpretation of structure probing *** the years computational rna biology has developed in which improved energy models and the availability of multiple genomes have contributed substantially to the development of the *** methods that search for common rna structures in a set of co-regulated rnas have

关键词： rna and roads towards further analysis Computational strategies to search structured rnas in genomic sequence

来源：评论

学校读者我要写书评

暂无评论

PTMD: A Database of Human Disease-associated Post-translational Modifications

引用

Genomics, Proteomics & Bioinformatics 2018年第4期16卷 244-251页

作者： Haodong Xu Yongbo Wang Shaofeng Lin Wankun Deng Di Peng Qinghua Cui Yu Xue Department of Bioinformatics & Systems Biology MOE Key Laboratory of Molecular Biophysics College of Life Science and Technology and the Collaborative Innovation Center for Biomedical Engineering Huazhong University of Science and Technology Department of Biomedical Informatics School of Basic Medical Sciences MOE Key Laboratory of Molecular Cardiovascular Sciences Center for Non-coding RNA Medicine Peking University

Various posttranslational modifications （PTMs） participate in nearly all aspects of biological processes by regulating protein functions, and aberrant states of PTMs are frequently implicated in human diseases. Therefore, an integral resource of PTM–disease associations （PDAs）would be a great help for both academic research and clinical use. In this work, we reported PTMD,a well-curated database containing PTMs that are associated with human diseases. We manually collected 1950 known PDAs in 749 proteins for 23 types of PTMs and 275 types of diseases from the literature. Database analyses show that phosphorylation has the largest number of disease associations, whereas neurologic diseases have the largest number of PTM associations. We classified all known PDAs into six classes according to the PTM status in diseases and demonstrated that the upregulation and presence of PTM events account for a predominant proportion of diseaseassociated PTM events. By reconstructing a disease–gene network, we observed that breast cancers have the largest number of associated PTMs and AKT1 has the largest number of PTMs connected to diseases. Finally, the PTMD database was developed with detailed annotations and can be a useful resource for further analyzing the relations between PTMs and human diseases. PTMD is freely accessible at http：//***.

关键词： Posttranslational modification Phosphorylation PTM-disease association Disease-gene network AKT1

来源：评论

学校读者我要写书评

暂无评论

Using GPU to accelerate the pairwise structural rna alignment with base pair probabilities

Using GPU to accelerate the pairwise structural RNA alignmen...

引用

作者： Sundfeld, Daniel Teodoro, George Havgaard, Jakob H. Gorodkin, Jan Melo, Alba C. M. A. Information and Communication Technology Brasília Federal Institute Brasília Brazil Department of Computer Science University of Brasília Brasília Brazil Center for Non-Coding RNA in Technology and Health Department of Veterinary and Animal Sciences University of Copenhagen Copenhagen Denmark

Structural alignments of Ribonucleic acid (rna) sequences solved by the Sankoff algorithm are computationally expensive and often require constraints to be used in practice. Modern Graphics Processing Units (GPUs) contain more than 1000 cores, which compute in parallel to speed up applications. Here, we present a GPU-based solution to the rna structural alignment problem that makes use of precalculated base pair probabilities on the individual sequences. We designed and developed an unconstrained version of the Sankoff algorithm, obtaining the optimal result and calculating the entire four-dimension dynamic programming matrix (4D DP). Our approach uses a two-level wavefront strategy to exploit parallelism. The 4D DP matrix is divided in one external matrix (EM) and several internal matrices (IM). We applied wavefront strategies on the EM and IMs in a two-level hierarchical way. At the first level, the wavefront is applied to the EM, calculating the cells that belong to the same diagonal in parallel. In the second level, since each cell in the EM is itself an IM matrix, the cells that belong to the same IM diagonal are calculated in parallel. The results obtained with real rna sequences show that our GPU version is capable of outperforming a multicore CPU version of the unconstrained version of the Sankoff algorithm. Compared with the CPU-based version running on 32 cores, our approach is able to achieve a speedup of 7.81x on the NVidia Tesla P100. In this case, the execution time was reduced from 6 hours and 18 minutes (32 cores) to 48 minutes and 20 seconds (GPU). © 2019 John Wiley & Sons, Ltd.

关键词： Graphics processing unit

来源：评论

学校读者我要写书评

暂无评论

SPARSE: Quadratic time simultaneous alignment and folding of rnas without sequence-based heuristics

SPARSE: Quadratic time simultaneous alignment and folding of...

引用

17th Annual International Conference on Research in Computational Molecular Biology, RECOMB 2013

作者： Will, Sebastian Schmiedl, Christina Miladi, Milad Möhl, Mathias Backofen, Rolf Bioinformatics Department of Computer Science University of Freiburg Germany Bioinformatics Department of Computer Science University of Leipzig Germany University of Freiburg Germany University of Freiburg Germany Centre for Non-coding RNA in Technology and Health Bagsvaerd Denmark

ISBN: (纸本)9783642371943

Motivation: There is increasing evidence of pervasive transcription, resulting in hundreds of thousands of ncrnas of unknown function. Standard computational analysis tasks for inferring functional annotations like clustering require fast and accurate rna comparisons based on sequence and structure similarity. The gold standard for the latter is Sankoff's algorithm [3], which simultaneously aligns and folds rnas. Because of its extreme time complexity of O(n6), numerous faster "Sankoff-style" approaches have been suggested. Several such approaches introduce heuristics based on sequence alignment, which compromises the alignment quality for rnas with sequence identities below 60% [1]. Avoiding such heuristics, as e.g. in LocArna [4], has been assumed to prohibit time complexities better than O(n 4), which strongly limits large-scale applications. © 2013 Springer-Verlag.

关键词： Alignment

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：